Effect of the incorporation of salted additives on probiotic whey cheeses

The research effort described here has focused on incorporation of Lactobacillus casei, in whey protein matrices, in the presence of selected salty additives. Those matrices were produced via thermal processing of a combination of either ovine or bovine whey (or a mixture thereof) with ovine milk, and were inoculated (at 10%) with L. casei strain LAFTI®L26; salt, salt and herbs, or salt and xanthan were further added to such matrices, which were then homogenized and stored at 7 °C for up to 21 d. In general, viable cell numbers maintained or even increased throughout the storage period, irrespective of the type of salty additive considered. Partial depletion of lactose was detected, and concomitant production of lactic acid throughout the 21 d-period of storage; lower lactic acid concentrations were found in matrices containing salty additives. In matrices with xanthan (SX), the probiotic strain exhibited the lowest metabolic activity. Matrices SX were less soft and firmer than the others, by the end of storage, and were similar to matrices with herbs (SH). The incorporation of salty additives affected bacterial metabolism, in terms of glycolysis and proteolysis, which in turn had a significant impact on the development of textural propertiesinfo:eu-repo/semantics/acceptedVersio

Niche differences may reduce susceptibility to competition between native and non‐native birds in oceanic islands

Aim Few bird extinctions on oceanic island have been attributed to competition with non-native species, even though it might be an overlooked driver of biodiversity loss. We evaluate the potential competition between native and non-native island bird species, identifying species and island characteristics that enhance it and may promote future extinctions. Location Seventy-three (>100 km2) oceanic islands worldwide. Methods We compiled a species list for each island and used single-trait meta-analyses to assess differences between native and non-native species. Then, we used single-trait beta regression models to identify species traits linked to potential competition. Finally, we used a trait-based approach to calculate the potential competition between native and non-native species on each island and identify island characteristics linked to potential competition. Results Native bird species tended to be smaller forest dwellers, that were either carnivore, frugivore or insectivore, and that foraged in flight, in the canopy or at mid-height. In contrast, non-native birds tended to be open habitat granivores, that were either ground or unspecialized foragers. Potential competition tended to be higher for native species with typical non-native traits and forest-dwelling unspecialized non-native species. Potential competition between native and non-native birds was consistently higher in islands that were larger, had more non-native birds or were drier. Main conclusions Niche differentiation of native and non-native species may explain the scarcity of reported competition-driven extinctions since non-natives clearly tend to favour and are better adapted to anthropogenic environments. However, the few non-native birds that occur in native ecosystems may be problematic. The loss of native ecosystems coupled with the introduction of species that might outcompete native species may enhance the relevance of competition in future island extinctions.info:eu-repo/semantics/publishedVersio

Angolan cymbopogon citratus used for therapeutic benefits: nutritional composition and influence of solvents in phytochemicals content and antioxidant activity of leaf extracts

Folk medicine is a relevant and effective part of indigenous healthcare systems which are, in practice, totally dependent on traditional healers. An outstanding coincidence between indigenous medicinal plant uses and scientifically proved pharmacological properties of several phytochemicals has been observed along the years. This work focused on the leaves of a medicinal plant traditionally used for therapeutic benefits (Angolan Cymbopogon citratus), in order to evaluate their nutritional value. The bioactive phytochemical composition and antioxidant activity of leaf extracts prepared with different solvents (water, methanol and ethanol) were also evaluated. The plant leaves contained ~60% of carbohydrates, protein (~20%), fat (~5%), ash (~4%) and moisture (~9%). The phytochemicals screening revealed the presence of tannins, flavonoids, and terpenoids in all extracts. Methanolic extracts also contained alkaloids and steroids. Several methods were used to evaluate total antioxidant capacity of the different extracts (DPPH; NO; and H2O2 scavenging assays, reducing power, and FRAP). Ethanolic extracts presented a significantly higher antioxidant activity (p < 0.05) except for FRAP, in which the best results were achieved by the aqueous extracts. Methanolic extracts showed the lowest radical scavenging activities for both DPPH; and NO; radicals

Sweet whey cheese matrices inoculated with the probiotic strain Lactobacillus paracasei LAFTI® L26

Consumption of dairy products containing viable probiotic strains has increased dramatically in recent years, owing to general health claims associated therewith. This trend has boosted diversification of the portfolio of said products, including whey cheese matrices. However, taking into account the relatively poor organoleptic and textural features of these matrices, improvement is in order via incorporation of selected additives, provided that viability of the strains is duly assayed. Lactobacillus paracasei LAFTI® L26 was accordingly incorporated into whey protein solid matrices, in the presence of several additives aimed at enhancing their organoleptic appeal and textural performance. These matrices were produced from a combination of either ovine or bovine whey (or a mixture thereof) with ovine milk, and were inoculated at 10% (v/v) with the probiotic strain. Sugar, sugar and aloe vera, sugar and chocolate, and sugar and jam were further added, and the resulting products were then stored at 7 ◦C for 21 d. In general, viable cell numbers remained high in all experimental matrices throughout storage. Despite the observed low extents of breakdown, proteolytic activities by the end of storage were higher in matrices containing jam. Furthermore, L. paracasei partially converted lactose into lactic acid in these matrices. Additives enhanced the organoleptic features of whey cheeses, and produced different textural patterns. The higher sensory scores were attained by matrices containing sugar: sugar and aloe vera received the best scores by 3 d of storage, but these scores decreased as storage time elapsed

Bird extinctions and introductions are causing taxonomic and functional homogenization in oceanic islands

Humans are quickly reshaping species assemblages through the loss and gain of species at multiple scales. Extinctions and introductions are non-random events known to be contributing to taxonomic homogenization. However, it is not yet clear if they also promote functional homogenization. Here, we assess whether extinctions and introductions are leading to taxonomic and functional homogenization of 64 oceanic island bird assemblages, belonging to 11 archipelagos. Based on island lists of extinct and extant, native and introduced species and on species traits, we use probabilistic hypervolumes in trait space to calculate functional beta-diversity before and after extinctions and introductions. Bird extinctions and introductions promoted taxonomic and functional homogenization on most oceanic islands. These results follow our expectations, considering previous studies on taxonomic homogenization, the predictable link between taxonomic and functional diversity, and the trait similarity of many introduced species, often adapted to anthropogenic habitats, linked to the non-randomness of bird introductions on islands. Taxonomic homogenization was more common across than within archipelagos, also corroborating previous studies describing stronger homogenization on islands that are further apart and thus had distinctive native assemblages. Surprisingly, the widespread loss of species with similar traits, namely large flightless birds, often led to functional differentiation across archipelagos. However, this differentiation effect tended to be offset by the homogenizing effect of introductions. Functional homogenization increases the vulnerability to global changes, by reducing the variability of responses to disturbance and thus the resilience of ecosystem services, posing a threat to human societies on islands. Our results highlight subtle variations in taxonomic and functional beta-diversity of bird assemblages in oceanic islands, providing important insights to allow a better assessment of how anthropogenic changes might alter ecosystem functioning, which is vital to develop effective long-term conservation strategies. Read the free Plain Language Summary for this article on the Journal blog.Peer reviewe

Antibacterial potential of northeastern Portugal wild plant extracts and respective phenolic compounds

The present work aims to assess the antibacterial potential of phenolic extracts, recovered from plants obtained on the North East of Portugal, and of their phenolic compounds (ellagic, caffeic, and gallic acids, quercetin, kaempferol, and rutin), against bacteria commonly found on skin infections. The disk diffusion and the susceptibility assays were used to identify the most active extracts and phenolic compounds. The effect of selected phenolic compounds on animal cells was assessed by determination of cellular metabolic activity. Gallic acid had a higher activity, against gram-positive (S. epidermidis and S. aureus) and gram-negative bacteria (K. pneumoniae) at lower concentrations, than the other compounds. The caffeic acid, also, showed good antibacterial activity against the 3 bacteria used. The gallic acid was effective against the 3 bacteria without causing harm to the animal cells. Gallic and caffeic acid showed a promising applicability as antibacterial agents for the treatment of infected wounds.The authors thank the Foundation for Science and Technology (Portugal) for financial support by the E. Pinho grant (SFRH/BD/62665/2009) and L. Barros grant (SFRH/BPD/4609/2000), and by the projects PEst-OE/EQB/LA0023/2013, PEst-C/CTM/UI0264/2011, and PEst-OE/EQB/LA0023/2013. Also, the authors acknowledge the financial support from FEDER through Programa Operacional Factores de Competitividade-COMPETE and to COMPETE/QREN/EU (CIMO strategic project 19 PEst-OE/AGR/UI0690/2011), and through the Project "BioHealth-Biotechnology and Bioengineering approaches to improve health quality", Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2-O Novo Norte), QREN, FEDER. The authors also acknowledge the project "Consolidating Research Expertise and Resources on Cellular and Molecular Biotechnology at CEB/IBB", Ref. FCOMP-01-0124-FEDER-027462

Earth-based render of tabique walls – an experimental work contribution

A research work focused on studying earth render for tabique application purposes is presented. Initially, a brief description of the tabique building technique is provided. The relevance of the application of this traditional building technique is also highlighted. Different compositions of earth render are experimentally analysed and the respective performance is evaluated. Flexural and compressive strengths, workability, drying shrinkage cracking and water resistance are the material properties assessed. A simple earth render is selected as being adequate for tabique building applications and it is applied on the manufacturing of a tabique wall sample. This wall sample is monitored in terms of thermal insulation ability and its thermal transmission coefficient is estimated. Taking into account that there is still a lack of published technical information related to this topic, this paper may contribute to solve this limitation and to give some guidance in future repairing processes of tabique construction. The technological benefit of adding lime or cement with earth is researched. Real tabique timber structure samples are applied in order to validate the obtained experimental results.info:eu-repo/semantics/publishedVersio

Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina

The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Neto, Emidio B.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Francisco, Aleff F.. Instituto de Biociencias; BrasilFil: Alfonso, Jorge. Fundación Oswaldo Cruz; BrasilFil: Soares, Andreimar. Fundación Oswaldo Cruz; BrasilFil: Pimenta, Daniel C.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Leiva, Laura Cristina Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentin

A Leishmania-specific hypothetical protein expressed in both promastigote and amastigote stages of Leishmania infantum employed for the serodiagnosis of, and as a vaccine candidate against, visceral leishmaniasis

Background: LiHyV is an antigenic hypothetical protein present in both promastigote and amastigote stages of Leishmania infantum, which was recently identified by an immunoproteomic approach. A recombinant version of this protein (rLiHyV) was evaluated as a diagnostic marker for canine VL (CVL). In addition, the prophylactic efficacy of the rLiHyV protein, and two of its CD8+ T cell epitopes, has been analyzed in a murine model of visceral leishmaniasis (VL). Methods: Initially, the rLiHyV protein was evaluated by an ELISA technique for the serodiagnosis of CVL. Secondly, vaccines composed of the recombinant protein and both chemically synthesized peptides, combined with saponin as an adjuvant; were administered subcutaneously into BALB/c mice. The cellular and humoral responses generated by vaccination were evaluated. In addition, the parasite burden and immune response were studied 10 weeks after L. infantum infection. Results: The rLiHyV protein was recognized by antibodies of VL dogs. No cross-reactivity was obtained with sera from dogs vaccinated with a Brazilian commercial vaccine, with sera from animals infected with Trypanosoma cruzi, Babesia canis and Ehrlichia canis, or those from non-infected animals living in an endemic area for leishmaniasis. After challenge with L. infantum, spleen cells of BALB/c mice vaccinated with rLiHyV/saponin stimulated with parasite antigens showed a higher production of IFN-γ, IL-12 and GM-CSF, than the same cells obtained from mice vaccinated with the individual peptides, or mice from control (inoculated with saline or saponin) groups. This Th1-type cellular response observed in rLiHyV/saponin vaccinated mice was accompanied by the induction of parasite-specific IgG2a isotype antibodies. Animals immunized with rLiHyV/saponin showed significant reductions in the parasite burden in the liver, spleen, bone marrow and in the lymph nodes draining the paws relative to control mice. Conclusions: The present study showed for the first time that the L. infantum LiHyV protein could be considered as a vaccine candidate against L. infantum infection, as well as a diagnostic marker for CVL.This work was supported by grants from Instituto Nacional de Ciência e Tecnologia em Nanobiofarmacêutica (INCT-Nanobiofar), FAPEMIG (CBB-APQ-00819-12), and CNPq (APQ-472090/2011-9, RHAE-456287/2012-4, APQ-482976/2012-8, and APQ-488237/2013-0). MACF is a grant recipient of FAPEMIG/CAPES. EAFC and APF are grant recipient of CNPq.Peer Reviewe