On the presence of the Ponto-Caspian hydrozoan Cordylophora caspia (Pallas, 1771) in an Iberian estuary: highlights on the introduction vectors and invasion routes

Several non-native invertebrate and vertebrate species have been detected in the Guadiana Estuary (SW-Iberian Peninsula, Europe) during the 21st century. In June 2015, the non-native hydroid Cordylophora caspia (Pallas, 1771) was detected for the first time in this estuary, which motivated an assessment of its distribution during late Spring and Summer 2016. The main goals of this paper were to: i) report the presence of Cordylophora caspia and its distribution in the Guadiana Estuary, ii) record the substrates colonized, salinity, and water temperatures at locations where the species was detected, iii) evaluate possible introduction vectors and invasion routes; and iv) discuss the potential impacts and management options. Cordylophora caspia occupied a 25-km stretch of the estuary with salinities between 0.2 and 13.8 and occupied a variety of human-made substrates. Shipping was the most likely introduction vector of C. caspia, which might have originated from populations in the Atlantic Ocean or the Mediterranean Sea. Currently, the potential ecological impacts are likely low since the population size is small due to an apparent shortage of suitable habitat. Economic effects are minimal at present because there are no major industries along the basin extracting water from the estuary. An integrated ecohydrological approach-i.e. freshets released from dams to control the populations of Cnidaria-was proposed to minimize or mitigate the potential negative effects of this species in the Guadiana Estuary.Delta Stewardship Council; Delta Science Program [1167]; Foundation for Science and Technology (FCT, Portugal) through the Jellyfisheries project [PTDC/MAR-BIO/0440/2014]; European Regional Development Fund (COMPETE program-Operational Competitiveness Programme); FCT [SFRH/BPD/108949/2015]; [UID/Multi/04326/2013]info:eu-repo/semantics/publishedVersio

The effect of freshwater pulse on Aurelia aurita (Linnaeus 1758) (Cnidaria: Scyphozoa): an ecohydrological solution for controlling jellyfish blooms

Dissertação de mestrado, Ecohidrologia, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2015Jellyfish blooms are increasingly being reported in coastal areas and have been related to both climate changes and anthropogenic impacts. However, several questions underlying such blooms remain understudied, particularly during their benthic phase (polyps). Salinity is one of the environmental variables that has been linked with blooms occurrence. Reports in Guadiana estuary have correlated high precipitation and high freshwater input to low medusa occurrence. Therefore, we hypothesize that using freshwater pulses from a hydrotechnical structure (Dam) could be a promising ecohydrological approach for controlling jellyfish blooms. To explore this idea, the present study aimed to assess different short term salinity concentrations effects on benthic stages and ephyrae larvae of Aurelia aurita. It also explored the feeding ecology of such stages. It had two set of experiment designs. The first set of experiments focuses on responses of scyphistomae (Experiment I, II ) and ephyrae larvae (Experiment III) under low salinity treatments (3,10,17 and 35(control)). The analyzed variables were survival, asexual reproduction and ecophysiological response (feeding rate and number of swimming pulses). It was found significant difference on scyphistomae survival between control and salinity 3 treatment (p-value < 0,05); on budding reproduction, number of scyphistoma actively budding and feeding response between control and salinity 10; on ephyrae survival between control and salinities 3 and 10 ; and ephyrae swimming pulses between control and salinity 17. It did not present significant difference on the number of strobilating scyphistoma and produced medusa. The second set of experiments investigated diet and feeding strategy of scyphistomae (Experiment IV) and ephyrae (Experiment V). It was used Costello plot method. The present study concluded that short term freshwater pulse may control jellyfish blooms by affecting jellyfish early phases survival, their ecophysiological response, and scyphistomae budding reproduction. It was also concluded that ephyrae and scyphistomae feeding strategy are generalists

Gelificação dos oceanos – e a culpa não é da alforreca

Ocean jellification is associated with increasing occurrence of jellyfishes. Jellyfish are gelatinous organisms, with a life cycle composed by sessile stage (polyp) and free living stage (medusa). Blooms jellies, which may have negative impact on fisheries and tourism, are strongly driven by the availability of food, since predators are relatively reduce. The diet of jellies includes small crustacean, egg and fish larvae. Yet, recent UAlg studies showed a much more diverse diet (ex: drifting microbes, terrestrial detritus). Major contributors for ocean jellification are closed connect to men activities and include: (1) excessive input of nutrients (ex: sewage agriculture fertilizers), (2) overfishing of fishes and sea turtles; (3) introduction of artificial structures and (4) changes in hydrological cycle (ex: river dams).info:eu-repo/semantics/publishedVersio

Effectiveness of a coordinated support system linking public hospitals to a health coaching service compared with usual care at discharge for patients with chronic low back pain: protocol for a randomised controlled trial

Background Although many people with chronic low back pain (LBP) improve following conservative treatment, one in five will experience worsening symptoms after discharge from treatment and seek health care again. The current LBP clinical care pathway in many health services lacks a well-integrated, systematic approach to support patients to remain physically active and self-manage their symptoms following discharge from treatment. Health coaching can support people to improve physical activity levels and may potentially reduce health care utilisation for LBP. The primary aim of this study is to evaluate the effect of introducing a coordinated support system (linking hospital outpatient physiotherapy services to a public health coaching service) at discharge from LBP treatment, on the future use of hospital, medical, and health services for LBP, compared with usual care provided at discharge. Methods Three hundred and seventy-four adults with chronic non-specific LBP will be recruited from the outpatient physiotherapy departments of public hospitals in New South Wales, Australia. Participants will be individually randomised to a support system (n = 187) or usual care group (n = 187). All participants will receive usual care provided at discharge from treatment. Participants allocated to the support system will also receive up to 10 telephone-based health coaching sessions, delivered by the Get Healthy Service®, over a 6-month period. Health coaches will monitor and support participants to improve physical activity levels and achieve personal health-related goals. The primary outcome is the total number of encounters with hospital, medical, and health services for LBP, at 12 months from baseline. A within-trial economic evaluation will quantify the incremental costs and benefits of the support system from a health system perspective, to support reimbursement decision making. Discussion This study will establish the effect of a coordinated support system, introduced at discharge from treatment, on the future use of hospital, medical, and health services for LBP and various health outcomes. Conclusion Innovative community-driven solutions to support people with chronic LBP after discharge from treatment are urgently needed. Study findings will help inform health care policy and clinical practice in Australia

Budding of filamentous and non-filamentous influenza A virus occurs via a VPS4 and VPS28-independent pathway

The mechanism of membrane scission during influenza A virus budding has been the subject of controversy. We confirm that influenza M1 binds VPS28, a subunit of the ESCRT-1 complex. However, confocal microscopy of infected cells showed no marked colocalisation between M1 and VPS28 or VPS4 ESCRT proteins, or relocalisation of the cellular proteins. Trafficking of HA and M1 appeared normal when endosomal sorting was impaired by expression of inactive VPS4. Overexpression of either isoform of VPS28 or wildtype or dominant negative VPS4 proteins did not alter production of filamentous virions. SiRNA depletion of endogenous VPS28 had no significant effect on influenza virus replication. Furthermore, cells expressing wildtype or dominant-negative VPS4 replicated filamentous and non-filamentous strains of influenza to similar titres, indicating that influenza release is VPS4-independent. Overall, we see no role for the ESCRT pathway in influenza virus budding and the significance of the M1-VPS28 interaction remains to be determined. (C) 2009 Elsevier Inc. All rights reserved

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Comprehensive Fragment Screening of the SARS-CoV-2 Proteome Explores Novel Chemical Space for Drug Development

12 pags., 4 figs., 3 tabs.SARS-CoV-2 (SCoV2) and its variants of concern pose serious challenges to the public health. The variants increased challenges to vaccines, thus necessitating for development of new intervention strategies including anti-virals. Within the international Covid19-NMR consortium, we have identified binders targeting the RNA genome of SCoV2. We established protocols for the production and NMR characterization of more than 80 % of all SCoV2 proteins. Here, we performed an NMR screening using a fragment library for binding to 25 SCoV2 proteins and identified hits also against previously unexplored SCoV2 proteins. Computational mapping was used to predict binding sites and identify functional moieties (chemotypes) of the ligands occupying these pockets. Striking consensus was observed between NMR-detected binding sites of the main protease and the computational procedure. Our investigation provides novel structural and chemical space for structure-based drug design against the SCoV2 proteome.Work at BMRZ is supported by the state of Hesse. Work in Covid19-NMR was supported by the Goethe Corona Funds, by the IWBEFRE-program 20007375 of state of Hesse, the DFG through CRC902: “Molecular Principles of RNA-based regulation.” and through infrastructure funds (project numbers: 277478796, 277479031, 392682309, 452632086, 70653611) and by European Union’s Horizon 2020 research and innovation program iNEXT-discovery under grant agreement No 871037. BY-COVID receives funding from the European Union’s Horizon Europe Research and Innovation Programme under grant agreement number 101046203. “INSPIRED” (MIS 5002550) project, implemented under the Action “Reinforcement of the Research and Innovation Infrastructure,” funded by the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014–2020) and co-financed by Greece and the EU (European Regional Development Fund) and the FP7 REGPOT CT-2011-285950—“SEE-DRUG” project (purchase of UPAT’s 700 MHz NMR equipment). The support of the CERM/CIRMMP center of Instruct-ERIC is gratefully acknowledged. This work has been funded in part by a grant of the Italian Ministry of University and Research (FISR2020IP_02112, ID-COVID) and by Fondazione CR Firenze. A.S. is supported by the Deutsche Forschungsgemeinschaft [SFB902/B16, SCHL2062/2-1] and the Johanna Quandt Young Academy at Goethe [2019/AS01]. M.H. and C.F. thank SFB902 and the Stiftung Polytechnische Gesellschaft for the Scholarship. L.L. work was supported by the French National Research Agency (ANR, NMR-SCoV2-ORF8), the Fondation de la Recherche Médicale (FRM, NMR-SCoV2-ORF8), FINOVI and the IR-RMN-THC Fr3050 CNRS. Work at UConn Health was supported by grants from the US National Institutes of Health (R01 GM135592 to B.H., P41 GM111135 and R01 GM123249 to J.C.H.) and the US National Science Foundation (DBI 2030601 to J.C.H.). Latvian Council of Science Grant No. VPP-COVID-2020/1-0014. National Science Foundation EAGER MCB-2031269. This work was supported by the grant Krebsliga KFS-4903-08-2019 and SNF-311030_192646 to J.O. P.G. (ITMP) The EOSC Future project is co-funded by the European Union Horizon Programme call INFRAEOSC-03-2020—Grant Agreement Number 101017536. Open Access funding enabled and organized by Projekt DEALPeer reviewe