A comprehensive review on role of phytochemicals in human health

Phytochemicals are plant extracts that are widely used due to their ability to provide health benefits and their therapeutic activity against several disorders. They are also called phytonutrients as they are not really the part of our diet but have several positive effects on ourhealth. They are found to decrease the risk factor of cardiovascular disorders and coronary heart disorders. It has also been found that phytochemicals diminish the risk ratio of several types of cancers including breast, colon and skin cancers. This article revolves around taking carotenoids, chlorophyll and fibers as examples of phytonutrients to evaluate their beneficial role in several diseases. These phytochemicals have been found to be associated with antioxidant activity, retinol activity, cell signaling, improvement of glycemic control, protection against high blood pressure, facilitation in defecation and anti-cancerous activity

Diagnostic Reliability of Paediatric Appendicitis Score & Ultrasound Scan in Children with Suspected Acute Appendicitis

Objective:  To evaluate the efficacy of Paediatric Appendicitis Score and ultrasound scan in the paediatric population. This study evaluates the best predictive measure for diagnosing acute appendicitis.Methodology: We conducted a prospective observational study on 120 children (02-13 years) in the department of Neonatal and Paediatric Surgery at The Children’s Hospital PIMS, Islamabad, with suspected appendicitis. Each patient was evaluated based on their Paediatric Appendicitis Score and ultrasound findings. We performed open appendectomy under general anaesthesia in patients with Paediatric Appendicitis Score ≥06 or who presented with severe tenderness on cough, TLC more than 10,000/mm3, or positive ultrasound findings.Results: In our study, the sensitivity and specificity of the Paediatric Appendicitis Score were 92.6% and 72.0%, respectively. Its sensitivity and specificity were 84.09% and 85.71%, respectively, with cutoff ≥6 combined with ultrasound findings. The combined sensitivity and specificity of Paediatric Appendicitis Score <6 and ultrasound were 85.71% and 94.44%, respectively.Conclusion: Paediatric Appendicitis Score is a useful diagnostic tool for suspected appendicitis in pediatric population. Its efficacy is significantly increased when combined with ultrasound. Combined application of the Paediatric Appendicitis Score with ultrasonography helps to rule out negative appendectomies and reduces complications associated with delayed diagnosis

Microbial Contamination in Drinking Water of Saggiyan-Lahore, Pakistan

The present study was carried to evaluate the physico-chemical parameters and microbial contamination of drinking water in the area of Saggiyan, Lahore. The tested water samples (S1-S30) were found to contain 199.6-402 mg/L TDS, 196-260 ppm EC, 196-260 ppm Hardness, 0.8-17 NTU turbidity, 169-290 mg/L alkalinity, 0-0.1 ppb arsenic and 0-0.1 ppm fluoride contents. 95% of the water samples were found contaminated with fecal coliforms while 27% of the samples had shown the presence of E. coli. Though the physico-chemical parameters of most water samples were found in the safe limits of WHO, however, the presence of microbial contamination rendered it non-suitable for drinking purposes

Effect of community-based kangaroo mother care package on neonatal mortality among preterm and low birthweight infants in rural Pakistan: Protocol for a cluster randomized controlled trial

Background: Neonatal mortality due to preterm birth and low birthweight remains a significant challenge in Pakistan. Kangaroo mother care (KMC) is a unique, low-cost intervention proven to reduce neonatal mortality and morbidity and increase exclusive breastfeeding rates. However, KMC has not been attempted in community settings in Pakistan. We aim to implement and evaluate the effectiveness of a community-based KMC package to reduce neonatal morbidity and mortality among preterm and low birthweight (LBW) infants, which will provide evidence for policy development and the large-scale implementation of KMC across the country.Objective: The primary objective of this trial is to reduce neonatal mortality among preterm and LBW infants. The secondary objectives are growth (measured as weight gain), reduced incidence of possible serious bacterial infection, and increased exclusive breastfeeding and continued breastfeeding practices.Methods: We designed a community-based cluster randomized controlled trial in one rural district of Pakistan. Stable, LBW babies (weighing 1200 grams to 2500 grams) are included in the study. The community KMC package, consisting of the KMC kit, information and counseling material, and community mobilization through KMC champions (village volunteers), was designed after preliminary research in the same geographical location and implemented in intervention clusters. The standard essential newborn care is offered in the control clusters. Infants are recruited and followed up by independent teams of data collectors. Data are collected on the duration of skin-to-skin contact, growth, breastfeeding practices, morbidities, neonatal mortality, and neurodevelopment status. Data analysis will be conducted based on the intention to treat principle. The Cox regression model will be used to assess the primary outcome of neonatal mortality. The secondary outcomes will be evaluated using linear or logistic regression.Results: The Ethics Review Committee of Aga Khan University, Pakistan, approved the study protocol in February 2017. Data collection began in August 2019 and will be completed in December 2021. Data analyses are yet to be completed.Conclusions: This intervention may be effective in preventing sepsis and subsequently improve survival in LBW newborns in Pakistan and other low-income and middle-income countries worldwide.Trial registration: clinicaltrials.gov NCT03545204; https://clinicaltrials.gov/ct2/show/NCT03545204.International registered report identifier (irrid): DERR1-10.2196/28156

Effect of bovine lactoferrin on seroconversion following polio vaccine administration in children: Protocol for a double-blinded randomised controlled trial

Introduction: The oral polio vaccine (OPV) has substantial results in eliminating wild poliovirus and the vaccine of choice in polio eradication. However, the mucosal immunity induced by the OPV is still uncertain. Literature has shown that bovine lactoferrin (BLF) is a safe and useful protein found in cow\u27s milk with extraordinary antimicrobial, antiviral, antiinflammatory and immune-modulatory functions that help children\u27s gut to fight against micro-organisms like poliovirus. However, limited data exist regarding the effect of BLF on polio vaccine immune response. The primary objective is to evaluate the effect of BLF in enhancing mucosal and humoral immunity in children following the administration of oral and inactivated polio vaccines.Methods and analysis: This is a two-arm double-blinded randomised controlled trial comparing 462 neonates (231 in both groups) receiving either BLF or placebo with breast milk. The intervention is administered from day 1 till 6 weeks of age to a full-term healthy singleton newborn born at the Aga Khan University Hospitals, Karachi, Pakistan. The primary outcome is the seroconversion, 1 month after the receipt of two doses of OPV (at 10 weeks). For descriptive statistical analysis, Stata will be used, the frequency with percentages will be reported to describe baseline characteristics of the participants. A χ2 test will be used to compare categorical variables and a simple t test to compare continuous variables. The proportion of seroconversion and shedding will be compared using χ2 test or Fisher\u27s exact test.Ethics and dissemination: The Ethics approval has been granted by the Ethics Review Committee (ERC) of Aga Khan University for the proposed trial (ID: 2019-1955-5013). Furthermore, the National Bioethics Committee (NBC) of Pakistan has also approved the study for human subject research (ID: 4-87/NBC-443/19/669). Study findings will be disseminated through presentations at scientific conferences and educational practice workshops and will be published in an international peer-reviewed scientific journal

Mulberry based zinc nano-particles mitigate salinity induced toxic effects and improve the grain yield and zinc bio-fortification of wheat by improving antioxidant activities, photosynthetic performance, and accumulation of osmolytes and hormones

Salinity stress (SS) is a challenging abiotic stress that limits crop growth and productivity. Sustainable and cost effective methods are needed to improve crop production and decrease the deleterious impacts of SS. Zinc (Zn) nanoparticles (NPs) have emerged as an important approach to regulating plant tolerance against SS. However, the mechanisms of SS tolerance mediated by Zn-NPs are not fully explained. Thus, this study was performed to explore the role of Zn-NPs (seed priming and foliar spray) in reducing the deleterious impacts of SS on wheat plants. The study comprised different SS levels: control, 6 and 12 dS m−1, and different Zn-NPs treatments: control, seed priming (40 ppm), foliar spray (20 ppm), and their combination. Salinity stress markedly reduced plant growth, biomass, and grain yield. This was associated with enhanced electrolyte leakage (EL), malondialdehyde (MDA), hydrogen peroxide (H2O2), sodium (Na), chloride (Cl) accumulation, reduced photosynthetic pigments, relative water contents (RWC), photosyntetic rate (Pn), transpiration rate (Tr), stomata conductance (Gs), water use efficiency (WUE), free amino acids (FAA), total soluble protein (TSP), indole acetic acid (IAA), gibberellic acid (GA), and nutrients (Ca, Mg, K, N, and P). However, the application of Zn-NPs significantly improved the yield of the wheat crop, which was associated with reduced abscisic acid (ABA), MDA, H2O2 concentration, and EL, owing to improved antioxidant activities, and an increase in RWC, Pn, Tr, WUE, and the accumulation of osmoregulating compounds (proline, soluble sugars, TSP, and FAA) and hormones (GA and IAA). Furthermore, Zn-NPs contrasted the salinity-induced uptake of toxic ions (Na and Cl) and increased the uptake of Ca, K, Mg, N, and P. Additionally, Zn-NPs application substantially increased the wheat grain Zn bio-fortification. Our results support previous findings on the role of Zn-NPs in wheat growth, yield, and grain Zn bio-fortification, demonstrating that beneficial effects are obtained under normal as well as adverse conditions, thanks to improved physiological activity and the accumulation of useful compounds. This sets the premise for general use of Zn-NPs in wheat, to which aim more experimental evidence is intensively being sought. Further studies are needed at the genomic, transcriptomic, proteomic, and metabolomic level to better acknowledge the mechanisms of general physiological enhancement observed with Zn-NPs application

Recent Research Trends in Genetic Algorithm Based Flexible Job Shop Scheduling Problems

Flexible Job Shop Scheduling Problem (FJSSP) is an extension of the classical Job Shop Scheduling Problem (JSSP). The FJSSP is known to be NP-hard problem with regard to optimization and it is very difficult to find reasonably accurate solutions of the problem instances in a rational time. Extensive research has been carried out in this area especially over the span of the last 20 years in which the hybrid approaches involving Genetic Algorithm (GA) have gained the most popularity. Keeping in view this aspect, this article presents a comprehensive literature review of the FJSSPs solved using the GA. The survey is further extended by the inclusion of the hybrid GA (hGA) techniques used in the solution of the problem. This review will give readers an insight into use of certain parameters in their future research along with future research directions

Efficient Congestion Minimisation by Successive Load Shifting in Multilayer Wireless Networks

Congestion in wireless networks is one of the major causes of system inefficiency, and with router load being the main contributor to overall network traffic flow, congestion is very dependent on the level of router load and how it can be effectively managed. This paper presents a novel low-complexity Successive Load Shifting (SLS) technique for intelligently shifting router load between network routers by predicting the probability of congestion occurrences in the network and exploiting the topology to reassign load to minimise congestion. Crucially, SLS does not compromise the data rate in avoiding congestion and is able to be seamlessly embedded into existing protocols with only a small increase incurred in system overheads. The performance of SLS has been extensively tested and critically evaluated using the widely adopted TCP and UDP protocols, with results confirming both significant throughput gains and superior packet loss performance

Quantitative ethnoveterinary study on plant resource utilization by indigenous communities in high-altitude regions

For millennia, ethnic knowledge has been intricately tied to local biodiversity and woven into the fabric of rural communities. Growing scientific evidence suggests that merging ethnic knowledge with new scientific findings can lead to socially acceptable and environmentally friendly approaches essential for the long-term prosperity of local communities. In the high-altitude region, where livestock raising is a key income source, and plant-based utilization for ethno-veterinary practices is widely practiced. In this context, this study was conducted with the aim of documenting the ethno-veterinary use of plant resources in different bio-geographical regions of Jammu and Kashmir's Himalayas (J & KH). Semi-structured interviews and group discussions were used to collect information. Principal component analysis (PCA) and Pearson correlation were conducted to analyze the data. We documented 148 species from 53 families that locals used for various purposes: medicine, fodder, tonic, antidote, magic, and also used to protect themselves from ectoparasite such as Pediculus humanus capitis by the local inhabitants. There were significant differences in the relative usage of plant resources across the three biogeographic regions. Comparatively, the highest number (41%) of plant species were used for ethnoveterinary in the Jammu region, while the lowest number (28%) of species were used in Kashmir. Across the regions, Kashmir and Jammu had the highest level of species similarity (17%), while Jammu and Ladakh had the lowest (1%). A cross-regional assessment of plant resources revealed that 18% of plants were shared among the regions. The reported use of Amaranthus blitum, Morus alba, Ficus palmata, Vitex negundo, Juniperus semiglobosa, Ulmus wallichiana, and Rumex nepalensis are novel for the ethno-veterinary uses of this part of the Himalayan region. The various dry unique traditional fodder preparations (gaaslov, gass khor, pan baath, kaandbaath, Lovgooad, Karb, and Phungma) from plant resources are reported for the first time from the Himalayan region and can be ascribed to the novelty of this study. Plant resources were not only a source of fodder and medicine but also presented themselves as an opportunity for livelihood generation. Therefore, our findings bridge the knowledge gap by documenting key ethnoveterinary applications of native plant species from the study region that are used to cure livestock diseases and disorders by the mountain inhabitants.This research was funded by project IGYMERA, under grant agreement 101030604. AM-d is a recipient of a postdoctoral Marie Curie fellowship under grant agreement 101030604 (IGYMERA).Peer reviewe

The possible 'calming effect' of subchronic supplementation of a standardised phospholipids carrier-based Melissa officinalis L. extract in healthy adults with emotional distress and poor sleep conditions: results from a prospective, randomized, double-blinded, and placebo-controlled clinical trial

Background: Emotional distress conditions such as depression, anxiety, stress, and poor sleep are widespread health problems that have a significant impact on people’s lives. Conventional drugs are commonly prescribed to treat emotional distress and poor sleep conditions; however, these medications have several limitations and have shown multiple side effects. Over recent years botanicals-based pharmacological agents have gained increasing research and clinical interest in the management of emotional distress and sleep disorder. Of note, Melissa officinalis L. (MO) leaf extract has demonstrated considerable neuropharmacological properties both in animal and human studies and has emerged as a promising natural “calming agent.” However, research in this area is limited, and more studies are needed to validate its efficacy in amelioration of emotional distress and poor sleep conditions. Objectives: We aimed to assess the pharmacological effects of subchronic supplementation of an innovative standardised phospholipid carrier-based MO aqueous extract on emotional distress and poor sleep conditions. Design: A 3-week prospective, randomised, placebo-controlled, parallel-group, double-blinded clinical trial was conducted in 100 healthy adults complaining of a moderate degree of depression, anxiety, or stress, with scores of ≥14, ≥10, and ≥19, respectively, in the self-report Depression, Anxiety, and Stress Scale (DASS-42) or poor sleep, as indicated by the score of >5 in the Pittsburgh Sleep Quality Index (PSQI) scale. In addition, the impact of emotional distress and/or poor sleep on participants’ mental wellbeing, emotional feelings, and quality of life was also assessed using the self-reported Warwick–Edinburgh Mental Wellbeing Scale (WEMWBS), Positive and Negative Affect Schedule (PANAS) scale, and quality of life (WHO-QoL-BREF) scale, respectively. Results: Oral supplementation of 200 mg of phospholipid-based MO aqueous extract (Relissa™) tablets twice a day (i.e., 400 mg/day) for 3 weeks led to significant improvements in the depressive mood, anxiety, stress, positive and negative affect (emotional feelings), overall mental wellbeing, and quality-of-life scores (all p values <0.001). Supplementation of MO extract was well tolerated, and no treatment-emergent effects or serious adverse events were reported. Conclusion: According to the results of this study, the phospholipid carrier-based MO aqueous extract possesses considerable neuropharmacological properties, and its supplementation may provide a promising therapeutic option for the management of moderate emotional distress and/or poor sleep conditions