A “Drug-Dependent” Immune System Can Compromise Protection against Infection: The Relationships between Psychostimulants and HIV

Psychostimulant use is a major comorbidity in people living with HIV, which was initially explained by them adopting risky behaviors that facilitate HIV transmission. However, the effects of drug use on the immune system might also influence this phenomenon. Psychostimulants act on peripheral immune cells even before they reach the central nervous system (CNS) and their effects on immunity are likely to influence HIV infection. Beyond their canonical activities, classic neurotransmitters and neuromodulators are expressed by peripheral immune cells (e.g., dopamine and enkephalins), which display immunomodulatory properties and could be influenced by psychostimulants. Immune receptors, like Toll-like receptors (TLRs) on microglia, are modulated by cocaine and amphetamine exposure. Since peripheral immunocytes also express TLRs, they may be similarly affected by psychostimulants. In this review, we will summarize how psychostimulants are currently thought to influence peripheral immunity, mainly focusing on catecholamines, enkephalins and TLR4, and shed light on how these drugs might affect HIV infection. We will try to shift from the classic CNS perspective and adopt a more holistic view, addressing the potential impact of psychostimulants on the peripheral immune system and how their systemic effects could influence HIV infection.Fil: Assis, Maria Amparo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnologia y Desarrollo.; Argentina. Universidad Nacional de Educación a Distancia; España. Universidad Nacional de Santiago del Estero. Facultad de Ciencias Medicas.; ArgentinaFil: Carranza, Pedro Gabriel. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina. Universidad Nacional de Santiago del Estero. Facultad de Ciencias Medicas.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo. - Universidad Nacional de Santiago del Estero. Instituto Multidisciplinario de Salud, Tecnología y Desarrollo; ArgentinaFil: Ambrosio, Emilio. Universidad Nacional de Educación a Distancia; Españ

H-RADIC: una solución de tolerancia a fallos para clústeres virtuales en ambientes multi-nube

Even though the cloud platform promises to be reliable, several availability incidents prove that it is not. How can we be sure that a parallel application finishes it´s execution even if a site is affected by a failure? This paper presents H-RADIC, an approach based on RADIC architecture, that executes parallel applications protected by RADIC in at least 3 different virtual clusters or sites. The execution state of each site is saved periodically in another site and it is recovered in case of failure. The paper details the configuration of the architecture and the experiment´s results using 3 clusters running NAS parallel applications protected with DMTCP, a very well-known distributed multi-threaded checkpoint tool. Our experiments show that by adding a cluster protector it will be possible to implement the next level in the hierarchy, where the first level in the RADIC hierarchy works as an observer at a site level. In adition, the experiments showed that the protection implementation is out of the critical path of the application and it depends on the utilized resources.Aunque las plataformas en la nube parecen ser muy confiables, varios incidentes de disponibilidad han podemos asegurarnos que una aplicación paralela termina su ejecución cuando el sitio en la nube ha sido afectado por una falla? Este articulo presenta HRADIC, un enfoque basado en la arquitectura RADIC, esta ejecuta aplicaciones paralelas en al menos 3 diferentes sitios o clústeres virtuales, todos protegidos por RADIC, donde el estado de la ejecución de cada sitio es guardado periódicamente en otro de los sitios y de ahí es recuperado en el caso de una falla. El articulo detalla la configuración de la arquitectura y los resultados de los experimentos usando 3 clústeres ejecutando aplicaciones NAS en paralelo, protegidas con DMTCP (una herramienta para realizar múltiples checkpoints). Nuestros experimentos muestran que al agregar un protector del clúster es posible implementar un nivel más en la jerarquía de RADIC, donde el primer nivel funciona como observador. Los experimentos muestran que la implementación de este protector esta fuera del camino critico de la ampliación y depende solamente de la utilización de recursos.Facultad de Informátic

H-RADIC: The Fault Tolerance Framework for Virtual Clusters on Multi-Cloud Environments

Even though the cloud platform promises to be reliable, several availability incidents prove that they are not. How can we be sure that a parallel application finishes the execution even if a site is affected by a failure? This paper presents H-RADIC, an approach based on RADIC architecture, that executes a parallel application in at least 3 different virtual clusters or sites. The execution state of each site is saved periodically in another site and it is recovered in case of failure. The paper details the configuration of the architecture and the experiments results using 3 virtual clusters running NAS parallel applications protected with DMTCP, a very well-known distributed multi-threaded checkpoint tool. Our experiments show that the execution time was increased between a 5% to 36% without failures and 27% to 66% in case of failures.Facultad de Informátic

H-RADIC: una solución de tolerancia a fallos para clústeres virtuales en ambientes multi-nube

Even though the cloud platform promises to be reliable, several availability incidents prove that it is not. How can we be sure that a parallel application finishes it´s execution even if a site is affected by a failure? This paper presents H-RADIC, an approach based on RADIC architecture, that executes parallel applications protected by RADIC in at least 3 different virtual clusters or sites. The execution state of each site is saved periodically in another site and it is recovered in case of failure. The paper details the configuration of the architecture and the experiment´s results using 3 clusters running NAS parallel applications protected with DMTCP, a very well-known distributed multi-threaded checkpoint tool. Our experiments show that by adding a cluster protector it will be possible to implement the next level in the hierarchy, where the first level in the RADIC hierarchy works as an observer at a site level. In adition, the experiments showed that the protection implementation is out of the critical path of the application and it depends on the utilized resources.Aunque las plataformas en la nube parecen ser muy confiables, varios incidentes de disponibilidad han podemos asegurarnos que una aplicación paralela termina su ejecución cuando el sitio en la nube ha sido afectado por una falla? Este articulo presenta HRADIC, un enfoque basado en la arquitectura RADIC, esta ejecuta aplicaciones paralelas en al menos 3 diferentes sitios o clústeres virtuales, todos protegidos por RADIC, donde el estado de la ejecución de cada sitio es guardado periódicamente en otro de los sitios y de ahí es recuperado en el caso de una falla. El articulo detalla la configuración de la arquitectura y los resultados de los experimentos usando 3 clústeres ejecutando aplicaciones NAS en paralelo, protegidas con DMTCP (una herramienta para realizar múltiples checkpoints). Nuestros experimentos muestran que al agregar un protector del clúster es posible implementar un nivel más en la jerarquía de RADIC, donde el primer nivel funciona como observador. Los experimentos muestran que la implementación de este protector esta fuera del camino critico de la ampliación y depende solamente de la utilización de recursos.Facultad de Informátic

H-RADIC: The Fault Tolerance Framework for Virtual Clusters on Multi-Cloud Environments

Even though the cloud platform promises to be reliable, several availability incidents prove that they are not. How can we be sure that a parallel application finishes the execution even if a site is affected by a failure? This paper presents H-RADIC, an approach based on RADIC architecture, that executes a parallel application in at least 3 different virtual clusters or sites. The execution state of each site is saved periodically in another site and it is recovered in case of failure. The paper details the configuration of the architecture and the experiments results using 3 virtual clusters running NAS parallel applications protected with DMTCP, a very well-known distributed multi-threaded checkpoint tool. Our experiments show that the execution time was increased between a 5% to 36% without failures and 27% to 66% in case of failures.Facultad de Informátic

H-RADIC: una solución de tolerancia a fallos para clústeres virtuales en ambientes multi-nube

Even though the cloud platform promises to be reliable, several availability incidents prove that it is not. How can we be sure that a parallel application finishes it´s execution even if a site is affected by a failure? This paper presents H-RADIC, an approach based on RADIC architecture, that executes parallel applications protected by RADIC in at least 3 different virtual clusters or sites. The execution state of each site is saved periodically in another site and it is recovered in case of failure. The paper details the configuration of the architecture and the experiment´s results using 3 clusters running NAS parallel applications protected with DMTCP, a very well-known distributed multi-threaded checkpoint tool. Our experiments show that by adding a cluster protector it will be possible to implement the next level in the hierarchy, where the first level in the RADIC hierarchy works as an observer at a site level. In adition, the experiments showed that the protection implementation is out of the critical path of the application and it depends on the utilized resources.Aunque las plataformas en la nube parecen ser muy confiables, varios incidentes de disponibilidad han podemos asegurarnos que una aplicación paralela termina su ejecución cuando el sitio en la nube ha sido afectado por una falla? Este articulo presenta HRADIC, un enfoque basado en la arquitectura RADIC, esta ejecuta aplicaciones paralelas en al menos 3 diferentes sitios o clústeres virtuales, todos protegidos por RADIC, donde el estado de la ejecución de cada sitio es guardado periódicamente en otro de los sitios y de ahí es recuperado en el caso de una falla. El articulo detalla la configuración de la arquitectura y los resultados de los experimentos usando 3 clústeres ejecutando aplicaciones NAS en paralelo, protegidas con DMTCP (una herramienta para realizar múltiples checkpoints). Nuestros experimentos muestran que al agregar un protector del clúster es posible implementar un nivel más en la jerarquía de RADIC, donde el primer nivel funciona como observador. Los experimentos muestran que la implementación de este protector esta fuera del camino critico de la ampliación y depende solamente de la utilización de recursos.Facultad de Informátic

Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry

Catecholamines dopamine, norepinephrine, and epinephrine are well-known neurotransmitters playing different roles in the nervous and endocrine system. These compounds are biologically synthesized in the phenylalanine-tyrosine pathway which consists on the successive conversion of L-phenylalanine into L-tyrosine, L-3,4-dihydroxyphenylalanine (L-DOPA), dopamine, norepinephrine, and epinephrine. This work describes the development of an enantioselective CE-ESI-MS2 methodology enabling, for the first time, the simultaneous enantioseparation of all the constituents involved in the Phe-Tyr metabolic pathway, since all these compounds except dopamine are chiral. The developed method was based on the use of a dual CDs system formed by 180 mM of methyl-beta-CD and 40 mM of 2-hydroxypropy1-beta-CD dissolved in 2 M formic acid (pH 1.2) and presented the advantage of avoiding the use of any time-consuming labelling procedure. LODs ranged from 40 to 150 nM and the unequivocal identification of the compounds investigated was achieved through their MS2 spectra. The applicability of this methodology to the analysis of biological samples (rat plasma) was also demonstrated. (C) 2016 Elsevier B.V. All rights reserved

Automatización y puesta en marcha de una línea "Tunnel Liner”

107 p.En este trabajo se desarrolla una memoria de aplicación en la industria, para este caso se automatizó y posteriormente se puso en marcha una línea de producción llamada “Tunnel Liner”, esta memoria se realizó en Cintac S.A.I.C. en donde procesan flejes de acero, la línea de producción es robusta en su parte mecánica pero era débil en la parte de control, debido a lo anterior se tomó la decisión de realizar un Update a la línea, para lo cual solicitaron a un estudiante de Ingeniería En Mecatrónica el cual realiza la automatización de la línea. En este trabajo se da a conocer la línea de producción antes y después de la automatización, a su vez existen tópicos importantes a la hora de poner en marcha cualquier tipo de maquina o línea de producción, es por esto, que se agregó la puesta en marcha en esta memoria, para poder así implementar lo aprendido en “Aplicación De Métodos De Mantenimiento”, complementado con “Diseño De Planes De Mantenimiento”. Tunnel Liner es un producto que se construye con planchas metálicas perforadas tanto lateralmente como en la punta y cola, esta plancha posee un largo determinado y dependiendo del diámetro requerido poseerá un ángulo de curvatura presente en cada plancha que al momento de armar generarán el tubo con el diámetro requerido por el cliente de Cintac. Para finalizar este resumen se debe dejar en claro que para entender claramente esta memoria se debe poseer un nivel mínimo de conocimiento en el área de electricidad, automatización y mantenimiento, ya que se utiliza en gran parte lenguaje técnico para dar a entender lo que se realizó en la memoria, debido a la complejidad del tema. /ABSTRACT: In this work develops a memory for application in industry, in this case was automated and then launched a product line called "Tunnel Liner" herein was made in Cintac SAIC where processed steel strip, the production line is robust in its mechanical but was weak in the control part, due to the above decision was made to perform an Update to the line, for which a student asked in engineering Mechatronics which performs line automation.In this work discloses the production line before and after automation in turn are important issues when starting any type of machine or production line, which is why, added commissioning motion herein, and to implement what they learned in "Maintenance Enforcement Methods", complete with "Design Maintenance Plans". Tunnel Liner is a product that is constructed with perforated metal plates both laterally and at the tip and tail, this plate has a length determined depending on the required diameter and possess a curvature angle present on each plate which generate when assembling the tube the diameter required by the client Cintac. To end this summary should make clear that to fully understand this specification must have a minimum level of knowledge in the field of electricity, automation and maintenance, as it is used largely technical language to mean what was done in memory, due to the complexity

Neuroimaging reveals distinct brain glucose metabolism patterns associated with morphine consumption in Lewis and Fischer 344 rat strains.

Vulnerability to addiction may be given by the individual's risk of developing an addiction during their lifetime. A challenge in the neurobiology of drug addiction is understanding why some people become addicted to drugs. Here, we used positron emission tomography (PET) and statistical parametric mapping (SPM) to evaluate changes in brain glucose metabolism in response to chronic morphine self-administration (MSA) in two rat strains with different vulnerability to drug abuse, Lewis (LEW) and Fischer 344 (F344). Four groups of animals were trained to self-administer morphine or saline for 15 days. 2-deoxy-2-[18F]-fluoro-D-glucose (FDG)-PET studies were performed on the last day of MSA (acquisition phase) and after 15 days of withdrawal. PET data were analyzed using SPM12. LEW-animals self-administered more morphine injections per session than F344-animals. We found significant brain metabolic differences between LEW and F344 strains in the cortex, hypothalamus, brainstem, and cerebellum. In addition, the different brain metabolic patterns observed after the MSA study between these rat strains indicate differences in the efficiency of neural substrates to translate the drug effects, which could explain the differences in predisposition to morphine abuse between one individual and another. These findings have important implications for the use of these rat strains in translational morphine and opiate research.MLS was supported by the Ministerio de Ciencia e Innovación, Instituto de Salud Carlos III (projects PI17/01766, and grant BA21/00030), co-funded by the European Regional Development Fund (ERDF), “A way to make Europe”, CIBERSAM, Delegación del Gobierno para el Plan Nacional sobre Drogas (2017/085), Fundación Mapfre, and Fundación Alicia Koplowitz. EA was supported by grants from Ministerio de Sanidad, Servicios Sociales e Igualdad and Red de Trastornos Adictivos del Instituto de Salud Carlos III (RTA-RD16/020/0022), Delegación del Gobierno para el Plan Nacional sobre Drogas (2016I073), Ministerio de Ciencia e Innovación (PSI2016-80541-P), UNED (Plan de Promoción de la Investigación 2018–2020), and the European Union´s Justice Programme – Drugs Policy Initiatives (JUST-2017-AG-DRUGS- 806996-JUSTSO). NLR was supported by Instituto de Investigación Sanitaria Gregorio Marañón, "Programa Intramural de Impulso a la I + D + I 2019″. MD work was supported by Ministerio de Ciencia e Innovación (MCIN) and Insituto de Salud Carlos III (ISCIII) (PT20/00044). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN), and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV‐2015‐0505).S

The effects of cocaine self-administration on dendritic spine density in the rat hippocampus are dependent on genetic background

Chronic exposure to cocaine induces modifications to neurons in the brain regions involved in addiction. Hence, we evaluated cocaine-induced changes in the hippocampal CA1 field in Fischer 344 (F344) and Lewis (LEW) rats, 2 strains that have been widely used to study genetic predisposition to drug addiction, by combining intracellular Lucifer yellow injection with confocal microscopy reconstruction of labeled neurons. Specifically, we examined the effects of cocaine self-administration on the structure, size, and branching complexity of the apical dendrites of CA1 pyramidal neurons. In addition, we quantified spine density in the collaterals of the apical dendritic arbors of these neurons. We found differences between these strains in several morphological parameters. For example, CA1 apical dendrites were more branched and complex in LEW than in F344 rats, while the spine density in the collateral dendrites of the apical dendritic arbors was greater in F344 rats. Interestingly, cocaine self-administration in LEW rats augmented the spine density, an effect that was not observed in the F344 strain. These results reveal significant structural differences in CA1 pyramidal cells between these strains and indicate that cocaine self-administration has a distinct effect on neuron morphology in the hippocampus of rats with different genetic backgrounds