Potential factors influencing the condition of demersal sharks in the Mediterranean deep sea ecosystems

The Alboran Sea and the Balearic Islands are two contrasting areas in terms of primary productivity. The former is among the most productive areas of the whole Mediterranean Sea, whereas the Balearic Islands is among the less productive ones in the western Mediterranean. The two areas also show different patterns in the bottom trawl fishing footprint. In the Alboran Sea, the fishing effort on the upper slope is much higher than in Balearic Islands. However, the opposite applies to the middle slope, the vast majority of it remaining unexploited in Alboran Sea, whereas a widespread fishery targeting blue and red shrimp Aristeus antennatus develops in Balearic Islands. Galeus melastomus is a small demersal shark species inhabiting slope bottoms, where adults and juveniles are segregated and concentrate in the middle and upper slope, respectively. It feeds intensively on mesopelagic preys which, in turn, feed on low trophic level organisms close to primary producers. In this work we aimed to study the effect of surface primary production on the condition of deep sea demersal communities. We compared the condition, diet and potential intra- and inter-species competition for feeding resources in the juveniles of G. melastomus between Alboran Sea and Balearic Islands. The comparisons of Le Cren’s relative condition factor, and hepatosomatic and digestivosomatic indices evidenced an opposite pattern to that a priori expectable: better condition in the less productive area. The analysis of stomach contents showed similar diets in both areas, but lower food ingestion in Alboran Sea than in Balearic Islands. Different abundances of potential intra- and inter-specific competitors for feeding resources in the two areas, apparently linked to the different fishing footprints, seems the most likely explanation for the better nourished individuals in Balearic Islands. Whereas juveniles of G. melastomus dominate the shark community in the Balearic Islands upper slope, where the presence of adults of this species is almost negligible, in Alboran Sea this stratum is dominated by adults and potential competitors of other small demersal shark species. All shark species together, including G. melastomus, showed abundances 38% higher in Alboran than in Balearic Islands. Our results show that condition of deep sea demersal fish is ruled by complex inter-relationships among different factors, needing the combination of biological, ecological and fisheries knowledge to understand its variability.En prensa2,42

Superficie y morfologia de los implantes de zirconio sinterizados

Investigaciones realizadas sobre pacientes portadores de implantes dentales de titanio, demostraron que la incidencia de alteraciones en los tejidos de soporte se vio influenciada por las características de su estructura superficial. El zirconio fue introducido como un material de reemplazo del titanio, para la elaboración de implantes dentarios, debido a sus buenas propiedades físicas. También la forma macroscópica de los implantes y sus características diferentes en función del diseño de los mismos (roscados, sin rosca, con sinterizado, etc), como así también las características de superficie de los mismos, pueden influir en los resultados posoperatorios.Facultad de Odontologí

Implantes de zirconio : Actualizacion y caracterización

Este Póster deviene de una mesa redonda y debate sobre la búsqueda de materiales estéticos y biocompatibles para implantes dentales que posean adecuada resistencia mecánica. Así surge el zirconio a fines de 1960 como material con aplicación odontológica, en 1970 comienza a desarrollarse el sistema CADCAM. El objetivo es demostrar la mejoría tanto en la estética como la biocompatibilidad que ofrecen estos tipos de implantes dentales.Facultad de Odontologí

Applying genomic approaches to delineate conservation strategies using the freshwater mussel Margaritifera margaritifera in the Iberian Peninsula as a model

Effective conservation actions to counteract the current decline of populations and species require a deep knowledge on their genetic structure. We used Single Nucleotide Polymorphisms (SNPs) to infer the population structure of the highly threatened freshwater pearl mussel Margaritifera margaritifera in the Iberian Peninsula. A total of 130 individuals were collected from 26 locations belonging to 16 basins. We obtained 31,692 SNPs through Genotyping by Sequencing (GBS) and used this dataset to infer population structure. Genetic diversity given as observed heterozygosity was low. Pairwise FST comparisons revealed low levels of genetic differentiation among geographically close populations. Up to 3 major genetic lineages were determined: Atlantic, Cantabrian and Douro. This structure suggests a close co-evolutionary process with brown trout (Salmo trutta), the primordial fish host of this mussel in the studied area. Some sub-basins showed some genetic structuring, whereas in others no intrapopulation differentiation was found. Our results confirm that genetic conservation units do not match individual basins, and that knowledge about the genetic structure is necessary before planning recovery plans that may involve relocation or restocking. The same reasoning should be applied to strictly freshwater species that are sessile or have restricted dispersal abilities and are currently imperiled worldwide.This work was supported by national funds of the Portuguese Foundation for Science and Technology (FCT) through the project MUSSELFLOW (contract PTDC/BIA- EVL/29199/2017) and under the strategic project LA/P/0069/2020 granted to the Associate Laboratory ARNET; and the grant awarded to C.S. Lima (MARE-ISPA/BI/004/2015).Peer reviewe

Adhesión de la microbiota oral en los procesos periimplantarios en presencia de implantes de zirconio

El rol de las bacterias en el desarrollo de la periodontitis, ha sido extensamente investigada por diferentes científicos, observando a la Porphyromona gingivalis y el Aggrigatebacter actinomycentenconmitans, como los microorganismos más patológicos y resistentes presentes en la periodontitis y en la periimplantitis.Facultad de Odontologí

Rugosidad superficial del zirconio para implantes dentarios y la adhesión de biofilm

El zirconio fue bien reconocido como un biomaterial estético y resistente para la fabricación de implantes dentarios. La biocompatibilidad que presenta en relación a los tejidos subyacentes a las piezas dentarias, también arrojaron excelentes resultados. En estudios de investigación en los que se comparó la adhesión del biofilm en el titanio y zirconio, la formación y adhesión del mismo fue escasa en el segundo material. El objetivo de este trabajo fue analizar la adhesión de biofilm en relación a la rugosidad superficial del óxido de zirconio. Para analizar la rugosidad superficial se utilizó el sistema EZEIMAGE a través Microscopía Electrónica de Barrido. Para la observación de las Unidades Formadoras de Colonias por mililitro (UFC/ml) adheridas a los cortes de zirconio se realizaron siembras con cepas activadas de cocos y bacilos orales, en medio de cultivo líquido agar sangre al 5 %, incubadas a 37 ºC en condiciones de anaerobiosis. Los resultados obtenidos indicaron que la rugosidad superficial en los cortes analizados no era significativa. Los datos obtenidos en relación al desarrollo del biofilm sobre las muestras del biomaterial, dieron un número estadísticamente no significativo de p>0,05. Por lo tanto, la fase experimental de este trabajo indica que las irregularidades que presenta el zirconio sobre su superficie no favorecen la adhesión del biofilm como otros materiales, pudiendo ser considerado para la confección de futuros implantes dentarios.The zirconium was recognized like sthetitc and resistent material to the manufacture of dental implants. The biocompatibility that presents in relation to the underlying tissue of teeth, also yielded excellent results. In research studies where adhesion of biofilm on titanium and zirconium were compared, its formation and addition was low in the second material. The aim of this proyect was analyze the addition of biofilm in relation to the superficial rugosity of zirconium oxide. To analyze the surface rugosity EZEIMAGE system was used sistem through Scanning Electron Microscopy. For observation of Colony Forming Units per milliliter (CFU/ml) attached to zirconium cuts was realized plantings strains of oral coccus and bacillus activated, in liquid medium crop blood agar at 5% incubated to 37°C in anaeorbic conditions. The obtained results indicated that the surface rugosity in the analyzed curts was not significant. The obtained data in relation to the development of biofilm on biomaterial samples, showed a statistically insignificant number of p> 0.05. Therefore, the experimental phase of this proyect indicates that the irregularities that presents zirconium on its surface do not favor the addition of biofilm like other materials, it could be considered to the confection of futures dental implants.Facultad de Odontologí

Rugosidad superficial del zirconio para implantes dentarios y la adhesión de biofilm

El zirconio fue bien reconocido como un biomaterial estético y resistente para la fabricación de implantes dentarios. La biocompatibilidad que presenta en relación a los tejidos subyacentes a las piezas dentarias, también arrojaron excelentes resultados. En estudios de investigación en los que se comparó la adhesión del biofilm en el titanio y zirconio, la formación y adhesión del mismo fue escasa en el segundo material. El objetivo de este trabajo fue analizar la adhesión de biofilm en relación a la rugosidad superficial del óxido de zirconio. Para analizar la rugosidad superficial se utilizó el sistema EZEIMAGE a través Microscopía Electrónica de Barrido. Para la observación de las Unidades Formadoras de Colonias por mililitro (UFC/ml) adheridas a los cortes de zirconio se realizaron siembras con cepas activadas de cocos y bacilos orales, en medio de cultivo líquido agar sangre al 5 %, incubadas a 37 ºC en condiciones de anaerobiosis. Los resultados obtenidos indicaron que la rugosidad superficial en los cortes analizados no era significativa. Los datos obtenidos en relación al desarrollo del biofilm sobre las muestras del biomaterial, dieron un número estadísticamente no significativo de p>0,05. Por lo tanto, la fase experimental de este trabajo indica que las irregularidades que presenta el zirconio sobre su superficie no favorecen la adhesión del biofilm como otros materiales, pudiendo ser considerado para la confección de futuros implantes dentarios.The zirconium was recognized like sthetitc and resistent material to the manufacture of dental implants. The biocompatibility that presents in relation to the underlying tissue of teeth, also yielded excellent results. In research studies where adhesion of biofilm on titanium and zirconium were compared, its formation and addition was low in the second material. The aim of this proyect was analyze the addition of biofilm in relation to the superficial rugosity of zirconium oxide. To analyze the surface rugosity EZEIMAGE system was used sistem through Scanning Electron Microscopy. For observation of Colony Forming Units per milliliter (CFU/ml) attached to zirconium cuts was realized plantings strains of oral coccus and bacillus activated, in liquid medium crop blood agar at 5% incubated to 37°C in anaeorbic conditions. The obtained results indicated that the surface rugosity in the analyzed curts was not significant. The obtained data in relation to the development of biofilm on biomaterial samples, showed a statistically insignificant number of p> 0.05. Therefore, the experimental phase of this proyect indicates that the irregularities that presents zirconium on its surface do not favor the addition of biofilm like other materials, it could be considered to the confection of futures dental implants.Facultad de Odontologí

Rugosidad superficial del zirconio para implantes dentarios y la adhesión de biofilm

El zirconio fue bien reconocido como un biomaterial estético y resistente para la fabricación de implantes dentarios. La biocompatibilidad que presenta en relación a los tejidos subyacentes a las piezas dentarias, también arrojaron excelentes resultados. En estudios de investigación en los que se comparó la adhesión del biofilm en el titanio y zirconio, la formación y adhesión del mismo fue escasa en el segundo material. El objetivo de este trabajo fue analizar la adhesión de biofilm en relación a la rugosidad superficial del óxido de zirconio. Para analizar la rugosidad superficial se utilizó el sistema EZEIMAGE a través Microscopía Electrónica de Barrido. Para la observación de las Unidades Formadoras de Colonias por mililitro (UFC/ml) adheridas a los cortes de zirconio se realizaron siembras con cepas activadas de cocos y bacilos orales, en medio de cultivo líquido agar sangre al 5 %, incubadas a 37 ºC en condiciones de anaerobiosis. Los resultados obtenidos indicaron que la rugosidad superficial en los cortes analizados no era significativa. Los datos obtenidos en relación al desarrollo del biofilm sobre las muestras del biomaterial, dieron un número estadísticamente no significativo de p>0,05. Por lo tanto, la fase experimental de este trabajo indica que las irregularidades que presenta el zirconio sobre su superficie no favorecen la adhesión del biofilm como otros materiales, pudiendo ser considerado para la confección de futuros implantes dentarios.The zirconium was recognized like sthetitc and resistent material to the manufacture of dental implants. The biocompatibility that presents in relation to the underlying tissue of teeth, also yielded excellent results. In research studies where adhesion of biofilm on titanium and zirconium were compared, its formation and addition was low in the second material. The aim of this proyect was analyze the addition of biofilm in relation to the superficial rugosity of zirconium oxide. To analyze the surface rugosity EZEIMAGE system was used sistem through Scanning Electron Microscopy. For observation of Colony Forming Units per milliliter (CFU/ml) attached to zirconium cuts was realized plantings strains of oral coccus and bacillus activated, in liquid medium crop blood agar at 5% incubated to 37°C in anaeorbic conditions. The obtained results indicated that the surface rugosity in the analyzed curts was not significant. The obtained data in relation to the development of biofilm on biomaterial samples, showed a statistically insignificant number of p> 0.05. Therefore, the experimental phase of this proyect indicates that the irregularities that presents zirconium on its surface do not favor the addition of biofilm like other materials, it could be considered to the confection of futures dental implants.Facultad de Odontologí

Omentin: a biomarker of cardiovascular risk in individuals with axial spondyloarthritis

ABSTRACT: Cardiovascular (CV) disease is the main cause of mortality in axial spondyloarthritis (axSpA). CV risk is enhanced by dysregulation of adipokines. Low omentin levels were associated with metabolic dysfunction and CV disease in conditions different from axSpA. Accordingly, we evaluated the genetic and functional implication of omentin in CV risk and subclinical atherosclerosis in a cohort of 385 axSpA patients. Subclinical atherosclerosis was evaluated by carotid ultrasound. Omentin rs12409609, in linkage disequilibrium with a polymorphism associated with CV risk, was genotyped in 385 patients and 84 controls. Serum omentin levels were also determined. omentin mRNA expression was assessed in a subgroup of individuals. Serum and mRNA omentin levels were lower in axSpA compared to controls. Low serum omentin levels were related to male sex, obesity, inflammatory bowel disease (IBD) and high atherogenic index. rs12409609 minor allele was associated with low omentin mRNA expression in axSpA. No association was observed with subclinical atherosclerosis at the genetic or functional level. In conclusion, in our study low omentin serum levels were associated with CV risk factors in axSpA. Furthermore, rs12409609 minor allele may be downregulating the expression of omentin. These data support a role of omentin as a CV risk biomarker in axSpA.We wish to thank all the patients and controls that participated in this study. This work was supported by funds of a NEXT-VAL grant (NVAL17/10) (Instituto de Investigación Sanitaria IDIVAL) awarded to FG. SR-M is supported by funds of the RETICS Program (RD16/0012/0009) from the ‘Instituto de Salud Carlos III´ (ISCIII), co-funded by the European Regional Development Fund (ERDF). VP-C is supported by a pre-doctoral grant from IDIVAL (PREVAL 18/01). VM is supported by funds of a Miguel Servet type I programme (grant CP16/00033) (ISCIII, co-funded by the European Social Fund (ESF)). LL-G is supported by funds of PI18/00042 (ISCIII, co-funded by ERDF). RL-M is a recipient of a Miguel Servet type I programme fellowship from the ISCIII, co-funded by the ESF (grant CP16/00033)

Implantes de zirconio : Actualización y caracterización

Este póster deviene de una mesa redonda y debate sobre la búsqueda de materiales estéticos y biocompatibles para  implantes dentales que posean adecuada resistencia mecánica. Así surge el zirconio a fines de 1960 como material con aplicación odontológica, en 1970 comienza a desarrollarse el sistema CAD CAM. Su objetivo es demostrar la mejoría tanto en la estética como la biocompatibilidad que ofrecen estos tipos de implantes dentales.Facultad de Odontologí