REFERENCING, GAINS-LOSSES ASYMMETRY AND NON-LINEAR SENSITIVITIES IN COMMUTER DECISIONS: ONE SIZE DOES NOT FIT ALL!

In contrast with expected utility theory, empirical findings indicate that decisionmakers are sensitive to departures from reference points rather than states. Several tests of the reference-dependent preference framework have been carried out in experimental economics, and to a smaller extent in a choice modelling setting, to date. However, these empirical applications have generally focussed on a single behavioural phenomenon using uniform modelling approaches. This paper aims to broaden existing work by presenting a multi-attribute framework, allowing contemporarily for gain-loss asymmetry, non-linearity and testing for several possible reference points. The framework is tested in the context of commuter choices and reveals important gains in model fit and further insights into behaviour compared to standard modelling approaches, including substantial impacts on implied welfare measures.Choice modeling, discrete choice experiment, reference effects, non-linearity, gain/loss deviations, commuting

Beyond Floodplain Analysis: A Modeler’s Experience Using HEC-RAS 2D for Spillway Assessments and Designs

HEC-RAS 5.0 (2D) has been increasingly used by the dam safety community for performing dam breach and other hydraulic analyses since its debut in 2015. While this two-dimensional hydraulic modeling software has wide applications in dam breach analysis and urban flood simulation, its ability to analyze complex multidirectional flow problems can also be used as a design tool for spillways, overtopping protection, and other hydraulic structures. In this manuscript, the authors discussed their experience using HEC-RAS and other two-dimensional hydraulic models to design and assess various hydraulic structures. This includes: 1) sizing spillway outlet channels and assessing the hydraulic adequacy of training dikes, especially where non-linear or super-elevated flow conditions are anticipated; 2) using depth, velocity, and shear stress outputs to design erosion/overtopping protection for vegetated spillways, lined channels, and earthen embankments; 3) designing temporary diversions to facilitate construction within rivers, reservoirs, or other waterways; and 4) identifying and assessing potential failure modes (e.g. erosion and headcutting of vegetated spillways). Insights are shared to help the audience understand when a two-dimensional modeling approach is effective and appropriate

Use of nanoencapsulated curcumin against vegetative cells and spores of Alicyclobacillus spp. in industrialized orange juice

Pathogenic and deteriorating bacteria are a great concern to food safety. In this sense, the present study evaluated the fight against microbial contamination through the use of nanoparticles containing curcumin, in addition to analyzing the physical properties of these nanoparticles. Efficient curcumin encapsulation was determined by Fourier transform infrared spectra evaluation and differential scanning calorimetry. Transmission electron microscopy images showed irregular shaped nanoparticles with broad size distribution (20–250 nm). The antibacterial activity was considered satisfactory, since curcumin in the form of nanoparticles demonstrated antimicrobial and antibacterial activity superior to curcumin in its free form, against both pathogenic bacteria, such as Staphylococcus aureus (MIC 125 μg/mL), and deteriorates, such as Alicyclobacillus acidoterrestris (MIC 62.5 μg/mL). Since curcumin nanoparticles may be consumed as a food additive, the bioactive properties of the nanoencapsulated curcumin were also evaluated in relation to antioxidant capacity (Thiobarbituric acid reactive substances (TBARS) and oxidative hemolysis inhibition assays) and cytotoxicity against four carcinoma cell lines, as well as two non-tumor cells. As a proof of concept, nanoparticles were incorporated in orange juice, with the juice maintaining satisfactory pH, °Brix, and color stability, during three days of storage (8 °C).This study was financed in part by the Coordenaç˜ao de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The authors thank the “Central Analítica Multiusu´ario da UTFPR Campo Mourão” (CAMulti-CM) for the analyses. Fernanda V. Leimann (process 039/2019) would like to thank Fundação Araucária (CP 15/2017- Programa de Bolsas de Produtividade em Pesquisa e Desenvolvimento Tecnológico) and CNPq (process number 421541/2018-0, Chamada Universal MCTIC/CNPq n◦ 28/2018). The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to CIMO (UIDB/00690/ 2020); L. Barros and C. Pereira also thank FCT, P.I., through the institutional scientific employment program-contract.info:eu-repo/semantics/publishedVersio

RESGATE E PROPAGAÇÃO VEGETATIVA DE Sequoia sempervirens

Objetivou-se com o estudo verificar resgate vegetativo, enraizamento de estacas e adaptação das mudas enraizadas em sistema de minijardim clonal de Sequoia sempervirens. Realizou-se a decepa de cinco indivíduos com 20 anos de idade e após foi contabilizada a capacidade de emissão de brotações. Com as brotações, foram confeccionadas estacas, as quais foram submetidas a diferentes concentrações de ácido indolacético (AIA) (0, 2000, 4000 e 6000 mg.L-1). Foram avaliadas a porcentagem de enraizamento e o número e comprimento de raízes 160 dias após o estaqueamento. O experimento foi instalado em DIC, com 5 repetições de 10 estacas. Após a formação das mudas com as estacas enraizadas, elas foram transplantadas para vasos, para a formação do minijardim clonal. O resgate vegetativo através da decepa de árvores apresentou potencialidade para produção de estacas de sequoia e revigoramento do material. O uso entre 2000 e 4000 mg.L-1 de AIA aumentou a porcentagem de enraizamento e também o número e comprimento de raízes formadas. O minijardim clonal de sequoia mostrou ser uma boa opção para a multiplicação clonal da espécie. A clonagem da sequoia pode auxiliar tanto no melhoramento convencional como em plantios comerciais da espécie, principalmente no Sul do Brasil, local onde encontra condições adequadas para seu crescimento.Palavras-chave: Silvicultura clonal; coníferas; rejuvenescimento; minijardim clonal. AbstractRescue and vegetative propagation of Sequoia sempervirens. The objective of the study to verify the vegetative rescue, rooting cuttings and rooted plants adapt in mini-clonal hedge of Sequoia sempervirens system. Was held coppicing five individuals aged 20 years old and was recorded after the ability to shoot emission. Shoots with different concentrations of indole acetic acid were applied (IAA) (0, 2000, 4000 and 6000 mg.L-1) in cuttings made by evaluating the percentage of rooting, number and length of roots 160 days after striking. The experiment was a completely randomized with five replicates of 10 cuttings. After the formation of seedlings with rooted cuttings, these were transplanted to pots for the formation of mini-clonal hedge. The vegetative rescue through coppicing trees showed potential for the production of cuttings sequoia and reinvigoration of the material. The use from 2000 to 4000 mg.L-1 IAA increased rooting percentage and the number and length of roots formed. The mini-clonal hedge of sequoia proved to be a good option for clonal multiplication of the species. Cloning of sequoia can help both conventional breeding and for commercial plantations of the species, especially in southern Brazil, where finds suitable conditions for their growth.Keywords: Clonal forestry; conifers; rejuvenation; mini-clonal hedge.The objective of the study to verify the vegetative rescue, rooting cuttings and rooted plants adapt in mini-clonal hedge of Sequoia sempervirens system. Was held coppicing five individuals aged 20 years old and was recorded after the ability to shoot emission. Shoots with different concentrations of indole acetic acid were applied (IAA) (0, 2000, 4000 and 6000 mg.L-1) in cuttings made by evaluating the percentage of rooting, number and length of roots 160 days after striking. The experiment was a completely randomized with five replicates of 10 cuttings. After the formation of seedlings with rooted cuttings, these were transplanted to pots for the formation of mini-clonal hedge. The vegetative rescue through coppicing trees showed potential for the production of cuttings sequoia and reinvigoration of the material. The use from 2000 to 4000 mg.L-1 IAA increased rooting percentage and the number and length of roots formed. The mini-clonal hedge of sequoia proved to be a good option for clonal multiplication of the species. Cloning of sequoia can help both conventional breeding and for commercial plantations of the species, especially in southern Brazil, where finds suitable conditions for their growth.Keywords: Clonal forestry; conifers; rejuvenation; mini-clonal hedge

Chemical characterization of marine fish of low‑commercial value and development of fish burgers

O objetivo deste trabalho foi caracterizar quimicamente o pescado marinho ubarana (Albula vulpes) e desenvolver hambúrguer de peixe. Foram preparadas três formulações de hambúrguer de peixe, com 5, 8 e 10% de fécula de mandioca e ingredientes funcionais (cebola, alho e pimenta-do-reino). Foram realizadas análises centesimais, microbiológicas e do pH da matéria-prima, assim como análises sensoriais dos hambúrgueres de peixe. O pescado ubarana apresentou rendimento e valor nutricional comparáveis aos de espécies de valor comercial, com elevado teor proteico e baixo teor de lipídios. Os teores de proteínas dos hambúrgueres de peixe variaram entre 17,52 e 19,40 g 100 g-1 (crus) e 20,74 e 24,25 g 100 g-1 (grelhados), e os de lipídios, entre 0,20 e 0,73 g 100 g-1 (crus) e 0,36 e 0,77 g 100 g-1 (grelhados). Nos testes sensoriais, as formulações receberam notas entre seis e sete na escala hedônica de nove pontos. Os índices de aceitação foram superiores a 70% para todos os atributos avaliados. Assim, o aproveitamento desta espécie subutilizada mostra-se viávelThe objective of this work was to perform the chemical characterization of marine bonefish (Albula vulpes) and the development of fish burgers. Three formulations of fish burgers were prepared, containing 5, 8, and 10% cassava starch and functional ingredients (onion, garlic, and ground white pepper). Proximate composition, microbiological and pH analyses of the raw material, and fish burgers were performed, as well as the sensory analyses of the fish burgers. The yield and nutritional value of bonefish was found to be comparable with those of species of commercial value, with high-protein and low-lipid contents. The protein content of the fish burgers varied from 17.52 to 19.40 g 100 g-1 (raw), and from 20.74 to 24.25 g 100 g-1 (grilled); and the lipid contents ranged from 0.20 to 0.73 g 100 g-1 (raw), and from 0.36 to 0.77 g 100 g-1 (grilled). In the sensory tests, the formulations received scores between six and seven on the nine-point hedonic scale. Acceptance indexes were higher than 70% for all the assessed attributes. Therefore, the use of this underutilized species proves to be feasible

Performance/outcomes data and physician process challenges for practical big data efforts in radiation oncology

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/146290/1/mp13136.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/146290/2/mp13136_am.pd

Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site

Maize ribosome-inactivating protein is classified as a class III or an atypical RNA N-glycosidase. It is synthesized as an inactive precursor with a 25-amino acid internal inactivation region, which is removed in the active form. As the first structural example of this class of proteins, crystals of the precursor and the active form were diffracted to 2.4 and 2.5 Å, respectively. The two proteins are similar, with main chain root mean square deviation (RMSD) of 0.519. In the precursor, the inactivation region is found on the protein surface and consists of a flexible loop followed by a long α-helix. This region diminished both the interaction with ribosome and cytotoxicity, but not cellular uptake. Like bacterial ribosome-inactivating proteins, maize ribosome-inactivating protein does not have a back-up glutamate in the active site, which helps the protein to retain some activity if the catalytic glutamate is mutated. The structure reveals that the active site is too small to accommodate two glutamate residues. Our structure suggests that maize ribosome-inactivating protein may represent an intermediate product in the evolution of ribosome-inactivating proteins. © 2007 The Author(s).published_or_final_versio