Endodontia: uma ou múltiplas sessões?

O tratamento endodôntico é um procedimento comum em medicina dentária, tradicionalmente é realizado em múltiplas sessões, com medicação intracanalar entre sessões, para reduzir ou eliminar os microrganismos e os seus produtos antes da obturação, mas o conceito de tratamento numa sessão não é novo e nos últimos anos tem sido mais incorporado na prática clínica. O uso de técnicas endodônticas e equipamentos contemporâneos têm revolucionado os procedimentos endodônticos de modo a que seja possível a realização do tratamento endodôntico em uma única sessão, não só por aumentarem a taxa de sucesso do tratamento endodôntico, mas também por reduzirem o tempo necessário para o tratamento. A realização do tratamento numa única sessão tem vindo a ganhar aceitação como sendo o melhor tratamento na maioria dos casos, sendo que alguns endodontistas acreditam que existem poucos casos que não possam ser tratados com sucesso em uma única sessão. Dada a tendência para uma sociedade cada vez com um ritmo mais acelerado, este tipo de tratamento tem-se tornado o tratamento de eleição e habitualmente o tipo de tratamento preferido pelos pacientes Este trabalho tem como objetivo fazer uma revisão sobre o debate da realização do tratamento endodôntico em uma ou múltiplas sessões, avaliando todas as vantagens e desvantagens da realização do tratamento endodôntico numa sessão, comparativamente ao tratamento endodôntico em múltiplas sessões, bem como as suas indicações e contraindicações, de modo a proporcionar ao médico dentista uma informação atualizada desta abordagem clínica.Root canal therapy is a common procedure in dentistry, and traditionally it is done in multiple sessions with medication within the root canal between sessions, to reduce or eliminate microorganisms and their products before the obturation. However, the concept of treatment in a single session is not new and in the past few years, it has been incorporated into the clinical practice. The use of endodontic techniques and contemporary equipment have revolutionized the endodontic procedures in order to make possible the realization of endodontic treatment in a single session, not only by increasing the success rate of endodontic treatment, but also reduce the time needed for treatment. The realization of a single treatment session has gained acceptance as the treatment of choice in most cases, and some endodontists believe that there are few cases that cannot be successfully treated in a single session. Given the trend towards ever faster pace with a society, this treatment has become the treatment of choice and normally the preferred type of treatment by patients The main purpose of this work if to make a review on the debate of endodontic treatment in one or multiple sessions. It will be evaluated the advantages and disadvantages of endodontic treatment in a single session and compare it to the endodontic treatment in multiple sessions, as well as their indications and contraindications. This way, it will be possible to give the dentist an updated information in this clinical approach

Endodontia: uma ou múltiplas sessões?

O tratamento endodôntico é um procedimento comum em medicina dentária, tradicionalmente é realizado em múltiplas sessões, com medicação intracanalar entre sessões, para reduzir ou eliminar os microrganismos e os seus produtos antes da obturação, mas o conceito de tratamento numa sessão não é novo e nos últimos anos tem sido mais incorporado na prática clínica. O uso de técnicas endodônticas e equipamentos contemporâneos têm revolucionado os procedimentos endodônticos de modo a que seja possível a realização do tratamento endodôntico em uma única sessão, não só por aumentarem a taxa de sucesso do tratamento endodôntico, mas também por reduzirem o tempo necessário para o tratamento. A realização do tratamento numa única sessão tem vindo a ganhar aceitação como sendo o melhor tratamento na maioria dos casos, sendo que alguns endodontistas acreditam que existem poucos casos que não possam ser tratados com sucesso em uma única sessão. Dada a tendência para uma sociedade cada vez com um ritmo mais acelerado, este tipo de tratamento tem-se tornado o tratamento de eleição e habitualmente o tipo de tratamento preferido pelos pacientes Este trabalho tem como objetivo fazer uma revisão sobre o debate da realização do tratamento endodôntico em uma ou múltiplas sessões, avaliando todas as vantagens e desvantagens da realização do tratamento endodôntico numa sessão, comparativamente ao tratamento endodôntico em múltiplas sessões, bem como as suas indicações e contraindicações, de modo a proporcionar ao médico dentista uma informação atualizada desta abordagem clínica.Root canal therapy is a common procedure in dentistry, and traditionally it is done in multiple sessions with medication within the root canal between sessions, to reduce or eliminate microorganisms and their products before the obturation. However, the concept of treatment in a single session is not new and in the past few years, it has been incorporated into the clinical practice. The use of endodontic techniques and contemporary equipment have revolutionized the endodontic procedures in order to make possible the realization of endodontic treatment in a single session, not only by increasing the success rate of endodontic treatment, but also reduce the time needed for treatment. The realization of a single treatment session has gained acceptance as the treatment of choice in most cases, and some endodontists believe that there are few cases that cannot be successfully treated in a single session. Given the trend towards ever faster pace with a society, this treatment has become the treatment of choice and normally the preferred type of treatment by patients The main purpose of this work if to make a review on the debate of endodontic treatment in one or multiple sessions. It will be evaluated the advantages and disadvantages of endodontic treatment in a single session and compare it to the endodontic treatment in multiple sessions, as well as their indications and contraindications. This way, it will be possible to give the dentist an updated information in this clinical approach

Intermittent ethanol binge exposure impairs object recognition but spares contextual and tone fear memory in adolescent rats

Adolescent brain development seems to be important for the maturation of brain structures and behavior. Intermittent binge ethanol drinking is common among adolescents, and this type of drinking can induce brain damage and cognitive deficits. In addition, emotional changes are frequently seen in alcoholics and rodents treated with ethanol. Considering the close relation between emotional arousal and cognitive responses, the present work investigates if intermittent ethanol binge exposure could differentially alter the performance of adolescent rats in aversive and non-aversive motivated tests. Male adolescent rats were submitted to ethanol treatment (2.5 or 5.0 g/Kg, o.a.) at 48-h intervals over postnatal day (PND) 30 to 60. Control animals were exposed to a similar administration protocol with saline administration. At PND61-PND63 animals were submitted to one-trial object recognition or contextual and tone fear conditioning paradigms. Binge ethanol drinking (at both 2.5 and 5.0 g/Kg) did not change freezing response in the contextual and tone fear conditioning. However, all doses impaired recognition rates 24h after training in object recognition test. In addition, despite a diminution of horizontal locomotion in the open field (only for the 5.0 g/Kg dose), no difference was detected regarding time in immobility, time in grooming and number of rearing in this paradigm. The present results show that the cognitive impairment resulting from intermittent binge ethanol exposure has a negative correlation with learning-associated emotional arousal.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Santa Casa de São Paulo Faculdade de Ciências MédicasUniversidade Federal de São Paulo (UNIFESP)UNIFESPSciEL

Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum

A kDNA PCR enzyme-linked immunosorbent assay (kDNA PCR-ELISA) for the diagnosis of human visceral leishmaniasis (HVL) was developed. The detection limit of the reaction, precision measurements, and cut-off of the kDNA PCR-ELISA were defined in a proof-of-concept phase. A reference strain of Leishmania (Leishmania) infantum and a bank of 14 peripheral blood samples from immunocompetent patients with VL were characterized using techniques considered gold standards, and 11 blood samples obtained from healthy individuals of an endemic area were also assessed. Phase II evaluation determined the performance of the assay in peripheral blood samples from 105 patients with VL (adults and children), 25 patients with Leishmania/HIV coinfection, 40 healthy individuals, and 33 asymptomatic individuals living in endemic areas. The kDNA PCR-ELISA exhibited satisfactory precision, with a detection limit of 0.07 fg of DNA from L. (L.) infantum and 1 parasite/mL blood. The overall sensitivity of the assay for all groups studied was 100% (95% confidence interval [CI]: 97.1–100%), and the specificity was 95% (95% CI: 83.5–98.6%). The kDNA PCR-ELISA was shown to be a useful tool for VL symptomatic and asymptomatic individuals diagnosis and its use in endemic countries may help monitor control interventions

The Starch Is (Not) Just Another Brick in the Wall: The Primary Metabolism of Sugars During Banana Ripening

The monocot banana fruit is one of the most important crops worldwide. As a typical climacteric fruit, the harvest of commercial bananas usually occurs when the fruit is physiologically mature but unripe. The universal treatment of green bananas with ethylene or ethylene-releasing compounds in order to accelerate and standardize the ripening of a bunch of bananas mimics natural maturation after increasing the exogenous production of ethylene. The trigger of autocatalytic ethylene production regulated by a dual positive feedback loop circuit derived from a NAC gene and three MADS genes results in metabolic processes that induce changes in the primary metabolism of bananas. These changes include pulp softening and sweetening which are sensorial attributes that determine banana postharvest quality. During fruit development, bananas accumulate large amounts of starch (between 15 and 35% w/w of their fresh weight, depending on the cultivar). Pulp softening and sweetening during banana ripening are attributed not only to changes in the activities of cell wall hydrolases but also to starch-to-sugar metabolism. Therefore, starch granule erosion and disassembling are key events that lead bananas to reach their optimal postharvest quality. The knowledge of the mechanisms that regulate sugar primary metabolism during banana ripening is fundamental to reduce postharvest losses and improve final product quality, though. Recent studies have shown that ethylene-mediated regulation of starch-degrading enzymes at transcriptional and translational levels is crucial for sugar metabolism in banana ripening. Furthermore, the crosstalk between ethylene and other hormones including indole-3-acetic acid and abscisic acid also influences primary sugar metabolism. In this review, we will describe the state-of-the-art sugar primary metabolism in bananas and discuss the recent findings that shed light on the understanding of the molecular mechanisms involved in the regulation of this metabolism during fruit ripening

PERFIL EPIDEMIOLÓGICO DE DOADORES DE SANGUE SOROPOSITIVOS PARA DOENÇA DE CHAGAS NA REGIÃO SUL

http://dx.doi.org/10.5902/2236583410789O presente estudo teve como objetivo conhecer o perfil epidemiológico dos doadores soropositivos para Doença de Chagas. Foram analisados os resultados da triagem sorológica de 25.207 doações no período de janeiro de 2004 a dezembro de 2007 no Serviço de Hemoterapia (SHT) do Hospital Universitário de Santa Maria (HUSM). Daqueles indivíduos que apresentaram resultado sorológico para Doença de Chagas, reagente ou indeterminado , foram avaliados retrospectivamente através dos dados obtidos através da entrevista prévia à doação. Entre os candidatos a doação com sorologia reagente para Trypanosoma cruzi (T.cruzi), 72,76% eram do sexo masculino com idade superior a 30 anos. Considerando o aspecto referente ao nascimento e residência, 36,99% das doações foram realizadas por indivíduos com suspeita sorológica para Doença de Chagas, nascidos e residentes em municípios da região considerados fora de risco para esta infecção. A prevalência de sorologia reagente ou com resultado indeterminado para Doença de Chagas foi de 0,98%.  A frequencia de descarte de hemocomponentes ocorreu devido sorologia reagente (63,01%) ou resultado indeterminado (36,99%). O desenvolvimento de testes mais específicos poderá vir a evitar a exclusão de um doador de sangue em potencial

Antifungal Activity of Decyl Gallate against Several Species of Pathogenic Fungi

This work aims to demonstrate that the gallic acid structure modification to the decyl gallate (G14) compound contributed to increase the antifungal activity against several species of pathogenic fungi, mainly, Candida spp., Cryptococcus spp., Paracoccidioides spp., and Histoplasma capsulatum, according to standardized microdilution method described by Clinical Laboratory Standard Institute (CLSI) documents. Moreover this compound has a particularly good selectivity index value, which makes it an excellent candidate for broad-spectrum antifungal prototype and encourages the continuation of subsequent studies for the discovery of its mechanism of action

Brazilian network for HIV Drug Resistance Surveillance (HIV-BresNet): a survey of treatment-naive individuals

Introduction: In Brazil, more than 487,450 individuals are currently undergoing antiretroviral treatment. In order to monitor the transmission of drug-resistant strains and HIV subtype distribution in the country, this work aimed to estimate its prevalence and to characterize the nationwide pretreatment drug resistance in individuals recently diagnosed with HIV between 2013 and 2015. Methods: The HIV threshold survey methodology (HIV-THS, WHO) targeting antiretroviral-naive individuals with recent HIV diagnosis was utilized, and subjects were selected from 51 highly populated cities in all five Brazilian macroregions. The HIV pol genotypic test was performed by genomic sequencing. Results: We analysed samples from 1568 antiretroviral-naive individuals recently diagnosed with HIV, and the overall transmitted drug resistance (TDR) prevalence was 9.5% (150 sequences). The regional prevalence of resistance according to Brazilian geographical regions was 9.4% in the northeast, 11.2% in the southeast, 6.8% in the central region, 10.2% in the north and 8.8% in the south. The inhibitor-specific TDR prevalence was 3.6% for nucleoside reverse transcriptase inhibitors (NRTIs), 5.8% for non-nucleoside reverse transcriptase inhibitors (NNRTIs) and 1.6% for protease inhibitors (PIs)1.0% of individuals presented resistance to more than one class of inhibitors. Overall, subtype B was more prevalent in every region except for the southern, where subtype C prevails. Conclusions: To the best of our knowledge, this is the first TDR study conducted in Brazil with nationwide representative sampling. The TDR prevalence revealed a moderate rate in the five Brazilian geographical regions, although some cities presented higher TDR prevalence rates, reaching 14% in Sao Paulo, for example. These results further illustrate the importance of surveillance studies for designing future strategies in primary antiretroviral therapy, aiming to mitigate TDR, as well as for predicting future trends in other regions of the globe where mass antiretroviral (ARV) treatment was implemented.Brazilian Ministry of HealthUniv Fed Rio de Janeiro, Lab Virol Mol, Dept Genet IB, Rio De Janeiro, RJ, BrazilFdn Med Trop Amazonas, Manaus, Amazonas, BrazilLAPI Univ Fed Bahia, Hosp Univ Prof Edgar Santos, Lab Pesquisa, Salvador, BA, BrazilLab Cent Saude Publ Ceara Lacen CE, Fortaleza, Ceara, BrazilLab Cent Saude Publ Dist Fed, Setor Grandes Areas Norte SGAN 601, Brasilia, DF, BrazilUniv Fed Minas Gerais UFMG, Fac Med, Lab Imunol & Biol Mol DIP, Belo Horizonte, MG, BrazilLab Cent Saude Publ Mato Grosso Sul, Campo Grande, MS, BrazilLab Cent Saude Publ Pernambuco, Recife, PE, BrazilLab Municipal Curitiba, Curitiba, PR, BrazilFiocruz MS, Lab AIDS & Imunol Mol, Dept Imunol, Rio De Janeiro, RJ, BrazilUniv Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Lab Carga Viral, Rio de Janeiro, RJ, BrazilInst Biol Exercito, Rio De Janeiro, RJ, BrazilLab Cent Saude Publ Rio Grande Sul, Porto Alegre, RS, BrazilLab Hosp Nossa Senhora Conceicao, Porto Alegre, RS, BrazilLab Cent Saude Publ Santa Catarina, Florianopolis, SC, BrazilUNESP, Lab Biol Mol Hemocentro Botucatu, Fac Med, Botucatu, SP, BrazilUniv Estadual Campinas, Lab Pesquisa AIDS, Hosp Clin, Campinas, SP, BrazilInst Adolfo Lutz Sao Jose do Rio Preto, Lab Biol Mol, Sao Jose Do Rio Preto, SP, BrazilUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Lab Retrovirol, Sao Paulo, SP, BrazilInst Adolfo Lutz Cent, Lab Retrovirus, Ctr Virol, Nucleo Doencas Sanguineas & Sexuais, Sao Paulo, SP, BrazilMinist Saude, Dept Vigilancia Prevencao & Controle DST AIDS & H, Setor Adm Fed Sul SAFS 02, Secretaria Vigilancia Saude, Brasilia, DF, BrazilUniv Brasilia, Programa Pos Grad Saude Colet, Fac Med, Fac Ciencias Saude, Brasilia, DF, BrazilUniv Sao Paulo, Fac Med, Sao Paulo, SP, BrazilUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, Lab Retrovirol, Sao Paulo, SP, BrazilBMH: TC 298/12Web of Scienc

Diagnosis, Prognosis and Treatment of Canine Cutaneous and Subcutaneous Mast Cell Tumors

Mast cell tumors (MCTs) are hematopoietic neoplasms composed of mast cells. It is highly common in dogs and is extremely important in the veterinary oncology field. It represents the third most common tumor subtype, and is the most common malignant skin tumor in dogs, corresponding to 11% of skin cancer cases. The objective of this critical review was to present the report of the 2nd Consensus meeting on the Diagnosis, Prognosis, and Treatment of Canine Cutaneous and Subcutaneous Mast Cell Tumors, which was organized by the Brazilian Association of Veterinary Oncology (ABROVET) in August 2021. The most recent information on cutaneous and subcutaneous mast cell tumors in dogs is presented and discussed

SARS-CoV-2 introductions and early dynamics of the epidemic in Portugal

Genomic surveillance of SARS-CoV-2 in Portugal was rapidly implemented by the National Institute of Health in the early stages of the COVID-19 epidemic, in collaboration with more than 50 laboratories distributed nationwide. Methods By applying recent phylodynamic models that allow integration of individual-based travel history, we reconstructed and characterized the spatio-temporal dynamics of SARSCoV-2 introductions and early dissemination in Portugal. Results We detected at least 277 independent SARS-CoV-2 introductions, mostly from European countries (namely the United Kingdom, Spain, France, Italy, and Switzerland), which were consistent with the countries with the highest connectivity with Portugal. Although most introductions were estimated to have occurred during early March 2020, it is likely that SARS-CoV-2 was silently circulating in Portugal throughout February, before the first cases were confirmed. Conclusions Here we conclude that the earlier implementation of measures could have minimized the number of introductions and subsequent virus expansion in Portugal. This study lays the foundation for genomic epidemiology of SARS-CoV-2 in Portugal, and highlights the need for systematic and geographically-representative genomic surveillance.We gratefully acknowledge to Sara Hill and Nuno Faria (University of Oxford) and Joshua Quick and Nick Loman (University of Birmingham) for kindly providing us with the initial sets of Artic Network primers for NGS; Rafael Mamede (MRamirez team, IMM, Lisbon) for developing and sharing a bioinformatics script for sequence curation (https://github.com/rfm-targa/BioinfUtils); Philippe Lemey (KU Leuven) for providing guidance on the implementation of the phylodynamic models; Joshua L. Cherry (National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health) for providing guidance with the subsampling strategies; and all authors, originating and submitting laboratories who have contributed genome data on GISAID (https://www.gisaid.org/) on which part of this research is based. The opinions expressed in this article are those of the authors and do not reflect the view of the National Institutes of Health, the Department of Health and Human Services, or the United States government. This study is co-funded by Fundação para a Ciência e Tecnologia and Agência de Investigação Clínica e Inovação Biomédica (234_596874175) on behalf of the Research 4 COVID-19 call. Some infrastructural resources used in this study come from the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT).info:eu-repo/semantics/publishedVersio