Impacts of Co-Solvent Flushing on Microbial Populations Capable of Degrading Trichloroethylene

With increased application of co-solvent flushing technologies for removal of nonaqueous phase liquids from groundwater aquifers, concern over the effects of the solvent on native microorganisms and their ability to degrade residual contaminant has also arisen. This study assessed the impact of ethanol flushing on the numbers and activity potentials of trichloroethylene (TCE)-degrading microbial populations present in aquifer soils taken immediately after and 2 years after ethanol flushing of a former dry cleaners site. Polymerase chain reaction analysis revealed soluble methane monooxygenase genes in methanotrophic enrichments, and 16S rRNA analysis identified Methylocystis parvus with 98% similarity, further indicating the presence of a type II methanotroph. Dissimilatory sulfite reductase genes in sulfate-reducing enrichments prepared were also observed. Ethanol flushing was simulated in columns packed with uncontaminated soils from the dry cleaners site that were dosed with TCE at concentrations observed in the field; after flushing, the columns were subjected to a continuous flow of 500 pore volumes of groundwater per week. Total acridine orange direct cell counts of the flushed and nonflushed soils decreased over the 15-week testing period, but after 5 weeks, the flushed soils maintained higher cell counts than the nonflushed soils. Inhibition of methanogenesis by sulfate reduction was observed in all column soils, as was increasing removal of total methane by soils incubated under methanotrophic conditions. These results showed that impacts of ethanol were not as severe as anticipated and imply that ethanol may mitigate the toxicity of TCE to the microorganisms

Moderate-vigorous physical activity and health-related quality of life among Hispanic/Latino adults in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL).

BackgroundPhysical activity is a modifiable healthy behavior that has been shown to positively influence health-related quality of life. However, research examining the link between physical activity and health-related quality of life among Hispanic/Latino adults is limited and inconsistent. The purpose of this study is to assess whether accelerometer-measured moderate-vigorous physical activity (MVPA) is associated with self-reported (a) mental health-related quality of life, and (b) physical health-related quality of life among diverse Hispanic/Latino adults in the US.MethodsCross-sectional data from 12,379 adults ages 18-74 years in 2008-2011, who participated in HCHS/SOL and had complete data were analyzed using complex survey design methods. Accelerometer data were categorized into no MVPA, low, moderate, and high MVPA. Health-related quality of life was assessed with the Short-Form 12 and we used the mental and physical component subscales where higher scores indicate better health-related quality of life. Multivariate linear regression models were used to derive adjusted means with 95% confidence intervals and linear trends.ResultsWe observed no significant linear trend between accelerometer-measured MVPA and mental health-related quality of life (ptrend = 0.73). There was a significant positive association between MVPA and physical health-related quality of life (ptrend < 0.001) where higher MVPA corresponded with higher scores in physical health-related quality of life. The adjusted means were 46.67 (44.85-48.48) for no MVPA, 49.33 (49.03-49.63) for low MVPA, 50.61 (50.09-51.13) for moderate MVPA, and 51.36 (50.86-51.86) for high MVPA.ConclusionsAmong diverse Hispanic/Latino adults in the US, accelerometer-measured MVPA was associated with physical health-related quality of life, but not mental health-related quality of life. Future interventions should evaluate if increases in MVPA lead to improvements in health-related quality of life

Análisis del régimen bomberil nacional aplicado en el municipio de Popayán

Existe una responsabilidad compartida en donde las entidades públicas y las instituciones; en este caso los cuerpos de bomberos que es el de servir y prevenir los riesgos de incendios, atención de rescates e incidentes de una manera integral, pero es también compromiso del Estado o entes territoriales brindar iniciativas para promover los recursos necesarios para el sostenimiento de esta actividad y lograr así la prestación de un buen servicio. Los cuerpos de bomberos en Colombia son entidades sin ánimo de lucro regidos actualmente por la Ley General de Bomberos en Colombia (Presidencia de la República de Colombia, 2012) ley 1575 de 2012 la cual deroga la Ley 322 del 4 de octubre de 1996

Enhancing Digital Twins of Semi-Automatic Production Lines by Digitizing Operator Skills

In recent years, Industry 4.0 has provided many tools to replicate, monitor, and control physical systems. The purpose is to connect production assets to build cyber-physical systems that ensure the safety, quality, and efficiency of production processes. Particularly, the concept of digital twins has been introduced to create the virtual representation of physical systems where both elements are connected to exchange information. This general definition encompasses a series of major challenges for the developers of those functionalities. Among them is how to introduce the human perspective into the virtual replica. Therefore, this paper presents an approach for incorporating human factors in digital twins. This approach introduces a methodology to offer suggestions about employee rotations based on their previous performance during a shift. Afterward, this method is integrated into a digital twin to perform human performance assessments to manage workers’ jobs. Furthermore, the presented approach is mainly comprised of a human skills modelling engine and a human scheduling engine. Finally, for demonstrating the approach, a simulated serial single-product manufacturing assembly line has been introduced.publishedVersionPeer reviewe

Dataset of characteristic remanent magnetization and magnetic properties of early Pliocene sediments from IODP Site U1467 (Maldives platform)

This data article describes data of magnetic stratigraphy and anisotropy of isothermal remanent magnetization (AIRM) from "Magnetic properties of early Pliocene sediments from IODP Site U1467 (Maldives platform) reveal changes in the monsoon system" [1]. Acquisition of isothermal magnetization on pilot samples and anisotropy of isothermal remanent magnetization are reported as raw data; magnetostratigraphic data are reported as characteristic magnetization (ChRM).info:eu-repo/semantics/publishedVersio

GRAVIDEZ NA ADOLESCÊNCIA: SIGNIFICADOS PARA A FAMÍLIA DE HOJE

This article presents the results of the study carried through with the objective to develop a theoretical model, whose central subjectis: the love and its components, and as sub subjects: the sensitivity, the perception, the interaction and the communication, among others, with the purpose to contribute for the strength of auto-esteem of a group of adolescents, with the support of its familiar groups and the health service team. It was carried through the implantation of workshops with the adolescents and the accompaniment of its familiar and agents of health. Forthe development of the work the information related to the demographic aspects had been collected and identified the risk and security factors of the group of adolescents, applied the self-esteem scale of Janda1 before and after application of the theoretical model. During the development of the theoretical model the participants had been interviewed. The results demonstrate the risks and security factors that live the adolescents in general, and had presented an addition to the values on self-esteem scale comparative with the initial measurement, the interviews revealed five categories related with the process of pregnancy lived by the adolescents: in search of identity; the familiar and social expectations interrupted; love and lovelessness in the interpersonal relations, influence of the feminine figure in the decisions, and the loving challenge as rescue of auto-esteem and expression of the feelings. The development of the workshops allowed the strengthening of the health agents with the pregnant adolescents through the sensitization and the reflection about the theme discussed, the concepts of the theoretical model of the loving challenge.El embarazo en la adolescente tiende a ser desaprobado en los contextos familiar y social, considerándose una conducta que se aleja de las expectativas familiares y sociales, lo que genera en la adolescente diferentes tipos de sentimientos. El presente artículo aborda la problemática de la familia frente a la adolescente embarazada, la cual se ha determinado a partir de la aplicación de un Modelo fundamentado en el amor 1. Estudio realizado con adolescentes grávidas de condición soioeconómica media baja de la ciudad de Cali. El estudio se realizó desde el enfoque cualitativoy de investigación acción y las adolescentes participantes estuvieron presentes desde del cuarto mes de gestación con edades entre 14 y 19 años, recolectando información relacionada con aspectos sociodemográficos; factores de riesgo y protectores; y entrevista en profundidad. Se aplicó el modelo a través de la implementación de talleres en los que tomaron parte además de las adolescentes los agentes de salud del área de influencia.Los hallazgos confirman los factores de riesgo y protectores que viven las adolescentes en general y las entrevistas en profundidad revelaron cinco categorías relacionadas con el proceso de embarazo vivido por las adolescentes y su familia: búsqueda de identidad, expectativas familiares y sociale interrumpidas, amor y desamor en las relaciones interpersonales, influencia de la figura femenina en las decisiones, y el desafío amoroso como rescate de autoestima y expresión de sentimientos. En este artículo nos referiremos a tres de ellas por considerarlas que son las que nos revelan con mayor detalle el significado de la gravidez en las adolescentes para la familia de hoy .Este artigo apresenta os resultados do estudo realizado com o objetivo de desenvolver um modelo teórico, cujo tema central é: o amor e seus componentes, e como sub temas: a sensibilidade, a percepção, a interação e a comunicação, entre outros, com a finalidade de contribuir para o fortalecimento da auto-estima de um grupo de adolescentes, com o apoio de seus grupos familiares e da equipe de saúde. Foi realizado através da implantação de oficinas com as adolescentes e do acompanhamento de seus familiares e de agentes de saúde. Para o desenvolvimentodo trabalho foram coletadas as informações relacionadas aos aspectos demográficos e se identificaram os fatores de risco e segurança do grupo de adolescentes, aplicou-se a escala de auto-estima de Janda 1 antes e após a aplicação do modelo. Durante o desenvolvimento do modelo foram realizadas entrevistas diretas com as participantes. Os achados demonstram os fatores de riscos e segurança que vivenciam as adolescentes em geral, e apresentaram um acréscimo aos valores da escala de auto-estima comparado com a medição inicial. As entrevistas diretas revelaram cinco categorias relacionadas com o processo de gravidez vivenciado pelas adolescentes: busca da identidade, EXPECTATIVAS FAMILIARES E SOCIAIS INTERROMPIDA, amor e desamor nas relações interpessoais, INFLUÊNCIA DA FIGURA FEMININA NAS DECISÕES, e o desafio amoroso como resgate da auto-estima e expressão dos sentimentos. O desenvolvimento das oficinas permitiu a interação dos agentes de saúde com as adolescentes grávidas através da sensibilização e a reflexão das temáticas abordadas, dos conceitos do modelo do desafio amoroso

A ADOLESCENTE GRÁVIDA: UM DESAFIO PARA A FAMÍLIA AO CONFRONTAR-SE COM SUA AUTO-ESTIMA

Investigación llevada a cabo con el propósito de aplicar un modelo teórico, que tiene como concepto central: el amor y sus componentes y como subconceptos: la sensibilidad, la percepción, la interacción y la comunicación, entre otros con el fin de contribuir al fortalecimiento de la autoestima de un grupo de adolescentes, con el apoyo de sus grupos familiares y del equipo de salud. Este modelo se llevó a cabo a través de la implementación de talleres con adolescentes, con acompañamiento de sus familiares y con agentes de salud. Se partió de la recolección de información relacionada con aspectos demográficos y se identificaron los factores de riesgo y protectores del grupo de adolescentes, igualmente se aplicó la escala de autoestima de Janda (1). Se realizaron entrevistas a las participantes. Los hallazgos confirman los factores de riesgo y protectores que viven las adolescentes y mostraron un incremento en los valores de la escala de autoestima comparado con la medición inicial. Las entrevistas revelaron cinco categorías relacionadas con el proceso de embarazo vivido por las adolescentes: búsqueda de identidad, expectativas familiares y sociales interrumpidas, amor y desamor en las relaciones interpersonales, influencia de la figura femenina en las decisiones, y el desafío amoroso como rescate de autoestima y expresión de sentimientos. El desarrollo de los talleres permitió fortalecer en los agentes de salud y adolescentes gravidas, a través de la sensibilización y reflexión de las temáticas abordadas, los conceptos del modelo del desafío amoroso.Results of the study which aimed to develop a theoretical model, whose central theme is: love and its components, and as sub-themes: the sensitivity, the perception, the interaction and the communication, among others, in order to contribute for selfesteem strengthening of a group of adolescents, with the support of their family members and health team.Workshops were carried out with the adolescents and their family members as well as health agents were followed up. Information related to the demographic aspects was collected and adolescents’ risk and safety factors were identified; Janda’s (1) self-esteem scale was applied. The participants were also interviewed. The results demonstrate the risks and safety factors that adolescents experience, and an increase in the values of the self-esteem scale was verified if compared to the initial measurement. The interviews disclosed five categories related to the pregnancy process lived by the adolescents: search for identity; interrupted family and social expectations; love and unconcern in the interpersonal relations, influence of the feminine figure in the decisions, and the love challenge as rescue of self-esteem and feeling expression. The workshops allowed the strengthening of health agents with the pregnant adolescents through sensitization and reflection on the discussed themes, the concepts of the theoretical model of love challenge.Resultados do estudo realizado com o objetivo de desenvolver um modelo teórico, cujo tema central é: o amor e seus componentes, e como subtemas, a sensibilidade, a percepção, a interação e a comunicação, entre outros, com a finalidade de contribuir para o fortalecimento da auto-estima de um grupo de adolescentes, com o apoio de seus grupos familiares e da equipe de saúde. Foi realizado através de oficinas com as adolescentes e do acompanhamento de seus familiares e de agentes de saúde. Foram coletadas as informações relacionadas aos aspectos demográficos e se identificaram os fatores de risco e segurança do grupo de adolescentes; aplicouse a escala de auto-estima de Janda (1). Foram realizadas também entrevistas diretas com as participantes. Os achados demonstram os fatores de risco e segurança que vivenciam as adolescentes, e apresentaram um acréscimo aos valores da escala de auto-estima, comparado com a medição inicial.As entrevistas revelaram cinco categorias relacionadas com o processo de gravidez vivenciado pelas adolescentes: busca da identidade, expectativas familiares e sociais interrompidas, amor e desamor nas relações interpessoais, influência da figura feminina nas decisões, e o desafio amoroso como resgate da auto-estima e expressão dos sentimentos. O desenvolvimento das oficinas permitiu o fortalecimento dos agentes de saúde com as adolescentes grávidas através da sensibilização e a reflexão das temáticas abordadas, dos conceitos do modelo do desafio amoroso

Osteomacs interact with megakaryocytes and osteoblasts to regulate murine hematopoietic stem cell function

Networking between hematopoietic stem cells (HSCs) and cells of the hematopoietic niche is critical for stem cell function and maintenance of the stem cell pool. We characterized calvariae-resident osteomacs (OMs) and their interaction with megakaryocytes to sustain HSC function and identified distinguishing properties between OMs and bone marrow (BM)–derived macrophages. OMs, identified as CD45+F4/80+ cells, were easily detectable (3%-5%) in neonatal calvarial cells. Coculture of neonatal calvarial cells with megakaryocytes for 7 days increased OM three- to sixfold, demonstrating that megakaryocytes regulate OM proliferation. OMs were required for the hematopoiesis-enhancing activity of osteoblasts, and this activity was augmented by megakaryocytes. Serial transplantation demonstrated that HSC repopulating potential was best maintained by in vitro cultures containing osteoblasts, OMs, and megakaryocytes. With or without megakaryocytes, BM-derived macrophages were unable to functionally substitute for neonatal calvarial cell–associated OMs. In addition, OMs differentiated into multinucleated, tartrate resistant acid phosphatase–positive osteoclasts capable of bone resorption. Nine-color flow cytometric analysis revealed that although BM-derived macrophages and OMs share many cell surface phenotypic similarities (CD45, F4/80, CD68, CD11b, Mac2, and Gr-1), only a subgroup of OMs coexpressed M-CSFR and CD166, thus providing a unique profile for OMs. CD169 was expressed by both OMs and BM-derived macrophages and therefore was not a distinguishing marker between these 2 cell types. These results demonstrate that OMs support HSC function and illustrate that megakaryocytes significantly augment the synergistic activity of osteoblasts and OMs. Furthermore, this report establishes for the first time that the crosstalk between OMs, osteoblasts, and megakaryocytes is a novel network supporting HSC function

Aging negatively impacts the ability of megakaryocytes to stimulate osteoblast proliferation and bone mass

Osteoblast number and activity decreases with aging, contributing to the age-associated decline of bone mass, but the mechanisms underlying changes in osteoblast activity are not well understood. Here, we show that the age-associated bone loss critically depends on impairment of the ability of megakaryocytes (MKs) to support osteoblast proliferation. Co-culture of osteoblast precursors with young MKs is known to increase osteoblast proliferation and bone formation. However, co-culture of osteoblast precursors with aged MKs resulted in significantly fewer osteoblasts compared to co-culture with young MKs, and this was associated with the downregulation of transforming growth factor beta. In addition, the ability of MKs to increase bone mass was attenuated during aging as transplantation of GATA1low/low hematopoietic donor cells (which have elevated MKs/MK precursors) from young mice resulted in an increase in bone mass of recipient mice compared to transplantation of young wild-type donor cells, whereas transplantation of GATA1low/low donor cells from old mice failed to enhance bone mass in recipient mice compared to transplantation of old wild-type donor cells. These findings suggest that the preservation or restoration of the MK-mediated induction of osteoblast proliferation during aging may hold the potential to prevent age-associated bone loss and resulting fractures