Causes and management of nutritional rickets among paediatric age group in Rajasthan: a randomised control trial

Background: Rickets disease frequently caused by insufficient of vitamin D. Nevertheless, subsequent researchers have attributed it to a calcium insufficiency throughout the eating plan. There is very little data on the relative efficacy of calcium, vitamin D, or both in the treatment of rickets. The goal of this study was to see if calcium, vitamin D, or a combination of the two may help young infants with nutritional rickets.Methods: 100 patients of nutritional rickets aged 6 months to 5 years were randomly assigned to receive vitamin D (6,00,000 IU single intramuscular injection), calcium (75 mg/kg/day elemental calcium orally) or a combination. All of the participants' demographics, nutritional status, dietary calcium, and phytate consumption were evaluated.Results: More than 80 percent of the patients in the study had 25OHD levels below 20 ng/ml. Rickets was discovered as an afterthought throughout the remaining 60. Lower respiratory illness (40), upper respiratory tract illness (10) and acute gastroenteritis have been the presenting conditions.Conclusions: Children experiencing rickets exhibited low vitamin D levels in their blood as well as a low calcium intake from their nutrition. When compared to either vitamin D or calcium alone, the combination showed the best therapeutic effect.

Differential protein expression analysis of activated E2F2-/-T lymphocytes by means of label-free quantification method

Comunicaciones a congreso

Análisis del efecto de la sobreexpresión o silenciamiento de ALY mediante Proteómica diferencial

Comunicaciones a congreso

Aly: a possible E2F interacting protein

Comunicaciones a congreso

Gel-free/label-free analysis to study the proteome of botrytis cinerea

Comunicaciones a congreso

Evaluating the functional outcomes of tibia and ipsilateral femur fracture, which are floating knee injuries, in adults

Background: High velocity trauma leads to ipsilateral femur and tibia fractures. Despite being very uncommon, they have a significant morbidity rate. Combinations of diaphyseal, metaphyseal, and complicated intra-articular fractures may be present in this kind of injury. Objectives were to assess the functional success of adult patients with ipsilateral femur and tibia fractures treated with various methods. Methods: The 30 adult patients with floating knee injuries underwent surgical treatment as part of the prospective research at the department of orthopedics, govt. medical college, and affiliated group of institutions in Kota. A combination of implants, such as an intra-medullary nail, locking plates, screws, or external fixators, were used to treat both femur and tibia fractures. Results: Karlstrom-Olerud criteria were used for the final evaluation. In our study, five patients (16.6%) had excellent outcomes, ten (33.3%) had good results, seven (23.3%), had acceptable results, and eight (26.6%) had poor results. Conclusions: When the fracture is diaphysis and it is treated with intra-medullary nails for both the femur and the tibia, the results are excellent. In this way, the age of the patient, the kind of fracture, the methods of fracture fixation, and the functional result were all taken into account

Label-free quantification based on data independent acquisition mass spectrometry

Comunicaciones a congreso

Validación de la técnica de análisis masivo ("shotgun") con péptidos no marcados para el estudio del proteoma de Botrytis cinerea

Comunicaciones a congreso

The intrinsically disordered distal face of nucleoplasmin recognizes distinct oligomerization states of histones

The role of Nucleoplasmin (NP) as a H2A-H2B histone chaperone has been extensively characterized. To understand its putative interaction with other histone ligands, we have characterized its ability to bind H3-H4 and histone octamers. We find that the chaperone forms distinct complexes with histones, which differ in the number of molecules that build the assembly and in their spatial distribution. When complexed with H3-H4 tetramers or histone octamers, two NP pentamers form an ellipsoidal particle with the histones located at the center of the assembly, in stark contrast with the NP/H2A-H2B complex that contains up to five histone dimers bound to one chaperone pentamer. This particular assembly relies on the ability of H3-H4 to form tetramers either in solution or as part of the octamer, and it is not observed when a variant of H3 (H3C110E), unable to form stable tetramers, is used instead of the wild-type protein. Our data also suggest that the distal face of the chaperone is involved in the interaction with distinct types of histones, as supported by electron microscopy analysis of the different NP/histone complexes. The use of the same structural region to accommodate all type of histones could favor histone exchange and nucleosome dynamics