Distal nucleotides affect the rate of stop codon read-through

Background: A key step in gene expression is the recognition of the stop codon to terminate translation at the correct position. However, it has been observed that ribosomes can misinterpret the stop codon and continue the translation in the 3 ′UTR region. This phenomenon is called stop codon read-through (SCR). It has been suggested that these events would occur on a programmed basis, but the underlying mechanisms are still not well understood. Methods: Here, we present a strategy for the comprehensive identification of SCR events in the Drosophila melanogaster transcriptome by evaluating the ribosomal density profiles. The associated ribosomal leak rate was estimated for every event identified. A statistical characterization of the frequency of nucleotide use in the proximal region to the stop codon in the sequences associated to SCR events was performed. Results: The results show that the nucleotide usage pattern in transcripts with the UGA codon is different from the pattern for those transcripts ending in the UAA codon, suggesting the existence of at least two mechanisms that could alter the translational termination process. Furthermore, a linear regression models for each of the three stop codons was developed, and we show that the models using the nucleotides at informative positions outperforms those models that consider the entire sequence context to the stop codon. Conclusions: We report that distal nucleotides can affect the SCR rate in a stop-codon dependent manner.Fil: Escobar, Luciana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; ArgentinaFil: Alonso, Andrés Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Ronderos, Jorge Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Diambra, Luis Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentin

Diseño del reforzamiento del pavimento flexible empleando la viga benkelman en la avenida Mansiche, tramo Mall Aventura Plaza – colegio Ramón Castilla.

El presente trabajo de investigación tiene como finalidad diseñar el reforzamiento estructural del pavimento flexible en la zona de estudio que es la Avenida Mansiche desde el Mall Aventura Plaza hasta el Colegio Ramon Castilla, para una proyección de tránsito vehicular de 20 años, evaluando el estado actual del pavimento mediante dos metodologías. La primera es la metodología del Índice de Condición del Pavimento PCI, este tipo de metodología es visual, la cual consiste en la inspección de las fallas presentes en la zona de estudio y categorizarlas de acuerdo al grado que presentan, obteniendo así un resultado de 53.15, indicando de tal manera que el estado del pavimento es REGULAR. La segunda metodología que se aplicó en el presente trabajo, es conocer el estado del pavimento por medio de las deflexiones obtenidas a través de la Viga Benkelman aplicando la metodología CONREVIAL, para así obtener el estado del resto de capas en este caso la subrasante que presenta la vía de estudio teniendo como resultado que el estado de la vía era MALO TIPO IV (MAL PAVIMENTO Y DEFICIENTE SUBRASANTE). Una vez obtenidos estos resultados, procedimos a realizar el diseño de reforzamiento estructural para un ESAL de diseño de 9,000,000.00, recomendando tener como espesores de reforzamiento estructural para el carril derecho un espesor de refuerzo de hormigón asfaltico de 80.12mm y para el carril izquierdo de 128.07mmThe purpose of this research work is to design the structural reinforcement of the flexible pavement in the study area, which is Mansiche Avenue from the Aventura Plaza Mall to the Ramon Castilla School, for a 20-year vehicular traffic projection, evaluating the current state of the pavement using two methodologies. The first is the Pavement Condition Index (PCI) methodology. This is a visual methodology, which consists of inspecting the faults present in the study area and categorising them according to their degree, thus obtaining a result of 53.15, indicating that the condition of the pavement is fair. The second methodology applied in this work is to determine the condition of the pavement by means of the deflections obtained through the Benkelman Beam applying the CONREVIAL methodology, in order to obtain the condition of the rest of the layers, in this case the subgrade of the road under study, with the result that the condition of the road was BAD TYPE IV (BAD PAVEMENT AND DEFICIENT SUBGRADE). Once these results were obtained, we proceeded to design the structural reinforcement for a design ESAL of 9,009,385.38, recommending a thickness of asphalt concrete reinforcement of 80.12mm for the right lane and 128.07mm for the left lane.Tesi

First-Principles Study of the Ferroelectric Properties of SrTaO2N/SrTiO3 Interfaces

First-principles calculations based on density-functional theory in the pseudo-potential approach have been performed for the total energy, crystal structure and cell polarization for SrTaO2N/SrTiO3 heterostructures. Different heterojunctions were analyzed in terms of the termination atoms at the interface plane, and periodic or non-periodic stacking in the perpendicular direction. The calculations show that the SrTaO2N layer is compressed along the ab-plane, while the SrTiO3 is elongated, thus favoring the formation of P4mm local environment on both sides of the interface, leading to net macroscopic polarization. The analysis of the local polarization as a function of the distance to the interface, for each individual unit cell was found to depend on the presence of a N or an O atom at the interface, and also on the asymmetric and not uniform c-axis deformation due to the induced strain in the ab-plane. The resulting total polarization in the periodic array was ≈0.54 C/m2, which makes this type of arrangement suitable for microelectronic applications.Fil: Bastidas Briceño, Ruby Carolina. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ciencias Básicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; ArgentinaFil: Fernandez, Victoria Ines. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; ArgentinaFil: Peltzer y Blanca, Eitel Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso, Roberto Emilio. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ciencias Básicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; Argentina. Universidad Nacional Arturo Jauretche; Argentin

Honeys from the Argentine Phytogeographic Provinces Chaqueña and Monte in Catamarca and La Rioja

The characterization of Argentinean honeys from the vegetation units (VUs) of the phytogeographical provinces located in Catamarca and La Rioja was performed with the analysis used for honey quality control: sugar profile, free acidity, pH, electrical conductivity (EC), color and contents of moisture, ash, total soluble solids, and hydroxymethylfurfural (HMF). Honeys were authentic and traceable, and complied with the specifications of national and/or international standards, verifying their blossom origin, high quality, good maturity, and freshness. Honeys from VU-9 and VU-23 presented significantly distinctive physicochemical parameters, evidencing the influence of the flora and pedoclimatic conditions of these VUs. Honeys from VU-23 presented significantly higher contents of glucose and total reducing sugars (F + G), and lower EC, ash contents, fructose/glucose ratio, and concentrations of turanose and maltose than those from VU-9. Honeys from VU-23 in La Rioja also exhibited characteristic lighter colors and lower pH and ºBrix values and turanose amounts; and honeys from VU-9 in La Rioja exhibited highest pH values. Catamarca honeys were characterized by high free acidity and high amounts of turanose, regardless of the VUs. The contents of sugars and total soluble solids, moisture, pH, EC, and color of honeys from Catamarca and La Rioja are published here for the first time. This study contributes to the typification of honeys from these provinces, which will provide them an added value and allow them to access newer markets.Fil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química. Grupo de Investigación en Ingeniería en Alimentos; ArgentinaFil: Aubone, Ines. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Amadei Enghelmayer, Marisa. Nexco, S.a.; ArgentinaFil: Rosso, Valeria Soledad. Nexco, S.a.; ArgentinaFil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones en Sanidad Producción y Ambiente. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Instituto de Investigaciones en Sanidad Producción y Ambiente; ArgentinaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentin

Characterization of Argentinian Honeys Based on their Sugar Profiles and Quality Parameters

The honeys from the Argentinian provinces of Buenos Aires, La Rioja,Catamarca and Misiones were characterised by the sugar profile (fructose, glucose,sucrose, turanose, maltose and erlose) and the physicochemical parameters (freeacidity, pH, electrical conductivity (EC), colour and contents of moisture, ash,total soluble solids and hydroxymethylfurfural (HMF)) used for honey qualitycontrol. Authentic and traceable honey samples (n = 572) collected along fiveharvests were analysed using the official analytical methods. All honeys met thespecifications of the national and/or international standards for the evaluatedparameters, which denoted the blossom origin of most honeys, and confirmedtheir high quality, good maturity and freshness. The influence of the flora andthe pedoclimatic conditions of each phytogeographical region on the physicaland chemical properties of honey allowed its characterization. Thus, BuenosAires produced typical lighter honeys, and Misiones and Catamarca, darker ones.Buenos Aires honeys presented particular lower values of pH, EC, ash, HMF andmaltose, and higher concentrations of erlose. Misiones honeys exhibited highervalues of moisture, free acidity, EC and turanose amounts, and lower contentsof fructose, glucose and total soluble solids. La Rioja honeys showed higherpH and ºBrix values; and Catamarca honeys, higher sucrose contents. To theauthors? knowledge, the sugar profiles and several quality parameters of honeysfrom Misiones and Catamarca are here reported for the first time, as well as anyphysical and chemical data on La Rioja honeys.Fil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Aubone, Ines. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Amadei Enghelmayer, Marisa. No especifíca;Fil: Rosso, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Müller, Pablo Fernando. Instituto Nuestra Señora de Itatí. Profesorado en Ciencias Agrarias y Protección Ambiental; ArgentinaFil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; Argentin

Genomic differentiation among varieties of Iberian pig

[EN] Aim of study: The objective of this study was to identify the autosomal genomic regions associated with genetic differentiation between three commercial strains of Iberian pig. Area of study: Extremadura (Spain). Material and methods: We used the Porcine v2 BeadChip to genotype 349 individuals from three varieties of Iberian pig (EE, Entrepelado; RR, Retinto; and TT, Torbiscal) and their crosses. After standard filtering of the Single Nucleotide Polymorphism (SNP) markers, 47, 67, and 123 haplotypic phases from EE, RR, and TT origins were identified. The allelic frequencies of 31,180 SNP markers were used to calculate the fixation index (FST) that were averaged in sliding windows of 2Mb. Main results: The results confirmed the greater genetic closeness of the EE and RR varieties, and we were able to identify several genomic regions with a divergence greater than expected. The genes present in those genomic regions were used to perform an Overrepresentation Enrichment Analysis (ORA) for the Gene Ontology (GO) terms for biological process. The ORA indicated that several groups of biological processes were overrepresented: a large group involving morphogenesis and development, and others associated with neurogenesis, cellular responses, or metabolic processes. These results were reinforced by the presence of some genes within the genomic regions that had the highest genomic differentiation. Research highlights: The genomic differentiation among varieties of the Iberian pig is heterogeneous along the genome. The genomic regions with the highest differentiation contain an overrepresentation of genes related with morphogenesis and development, neurogenesis, cellular responses and metabolic processes.Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA), Spain RTA2012-00054-C02-01 Ministry of Science, Innovation and Universities, Spain CGL2016-80155-R; IDI-20170304 (CDTI)Alonso, I.; Ibáñez-Escriche, N.; Noguera, JL.; Casellas, J.; Martin De Hijas-Villalba, M.; Gracia-Santana, MJ.; Varona, L. (2020). Genomic differentiation among varieties of Iberian pig. Spanish Journal of Agricultural Research (Online). 18(1):1-20. https://doi.org/10.5424/sjar/2020181-15411120181Alexander DH, Novembre J, Lange K, 2009. Fast model-based estimation of ancestry in unrelated individuals. Genome Res 19: 1655-1664.Audetat KA, Galbraith MD, Odell AT, Lee T, Pandey A, Espinosa JM, Dowell RD, Taatjes D J, 2017. A kinase-independent role for cyclin-dependent kinase 19 in p53 response. Mol Cell Biol 37: e00626-16.Cepica S, Ovilo, C, Masopust M, Knoll A, Fernández A, López A, Rohrer GA, Nonneman D, 2012. Four genes located on a SSC2 meat quality QTL region are associated with different meat quality traits in Landrace x Chinese-European crossbred population. Anim Genet 43: 333-336.Conaway RC, Conaway JW, 2009. The INO80 chromatin remodeling complex in transcription, replication and repair. Trends Biochem Sci 34: 71-77.Correa RG, Krajewska M, Ware CF, Gerlic M, Reed JC, 2014. The NLR-related protein NWD1 is associated with prostate cancer and modulates androgen receptor signaling. Oncotarget 30: 1666-1682.Fabuel EC, Barragán C, Silio L, Rodríguez MC, Toro MA, 2004. Analysis of genetic diversity and conservation priorities in Iberian pigs based on microsatellite markers. Heredity 93: 104-113.Fontanesi L, Schiavo G, Galimberti G, Bovo S, Russo V, Gallo M, Buttazzoni L, 2017. A genome-wide association study for a proxy of intermuscular fat level in the Italian Large White breed identifies genomic regions affecting an important quality parameter for dry-cured hams. Anim Genet 48: 459-465.Hérault Y, Hraba-Renevey S, van der Hoeven F, Duboule D, 1997. Function of the Evx-2 gene in the morphogenesis of vertebrate limbs. EMBO J 15: 6727-6738.Herrero-Medrano JM, Megens HJ, Groenen MAM, Ramis G, Bosse M, Pérez-Enciso M, Crooijmans RPMA, 2013. Conservation genomic analysis of domestic and wild pig populations from the Iberian Peninsula. BMC Genet 14: 106.Izu Y, Sun M, Zwolanek D, Veit G, Williams V, Cha B, Jepsen KJ, Koch M, Birk DE, 2011. Type XII collagen regulates osteoblast polarity and communication during bone formation. J Cell Biol 193: 1115-1130.Jeyabal PVS, Rubio V, Chen H, Zhang J, Shi ZZ, 2014. Regulation of cell-matrix adhesion by OLA1, the Obg-like ATPase 1. Biochem Biophys Res Commun 444: 568-574.Kawakami Y, Rodríguez-Esteban C, Matsui T, Rodríguez-León J, Kato S, Izpisúa-Belmonte JC, 2004. Sp8 and Sp9, two closely related buttonhead-like transcription factors, regulate Fgf8 expression and limb outgrowth in vertebrate embryos. Development 131: 4763-4774.Laval G, Iannucelli N, Legault C, Milan D, Groenen MAM, Giuffra E, Andersson L, Nissen PH, Jorgensen CB, Beeckmann P et al., 2000. Genetic diversity of eleven European pig breeds. Genet Sel Evol 32: 187-203.Lim HH, Michael GJ, Smith P, Lim L, Hall C, 1992. Developmental regulation and neuronal expression of the mRNA of rat n-chimaerin, a p21rac GAP:cDNA sequence. Biochem J 287: 415-422.Marchand M, Schroeder IS, Markossian S, Skoudy A, Nègre D, Cosset FL, Real P, Kaiser C, Wobus AM, Savarier P, 2009. Mouse ES cells over-expressing the transcription factor NeuroD1 show increased differentiation towards endocrine lineages and insulin-expressing cells. Int J Dev Biol 53: 569-578.Martínez AM, Delgado JV, Rodero A, Vega-Pla JL, 2000. Genetic structure of the Iberian pig breed using microsatellites. Anim Genet 31: 295-301.Myers P, 2008. Hox genes in development: the HOX code. Nature Education 1: 2.Onteru SK, Fan B, Nikkilä MT, Garrick DJ, Stalder KJ, Rothschild MF, 2011. Whole-genome association analyses for lifetime reproductive traits in pig. J Anim Sci 89: 988-995.Onteru SK, Fan B, Du ZQ, Garrick DJ. Stalder KJ, Rothschild MF, 2012. A whole-genome association study for pig reproductive traits. Anim Genet 43: 18-26.Pallares LF, Carbonetto P, Gopalakrishnan S, Parker CC, Ackert-Bicknell CL, Palmer AA, Tautz D, 2015. Mapping of craniofacial traits in outbred mice identifies major developmental genes involved in shape determination. Plos Genet 11: e1005607.Ponsuksili S, Zebunke M, Murani E, Trakooljul N, Krieter J, Puppe B, Schwerin M, Wimmers K, 2015. Integrated genome-wide association and hypothalamus eQTL studies indicate a link between the circadian rhythm-related gene PER1 and coping behavior. Sci Rep 5: 16264.Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MAR, Bender D, Maller J, Sklar P, de Bakker PIW, Daly MJ, Sham PC, 2007. PLINK: a tool set for whole-genome association and population-based linkage analysis. Am J Human Genet 81: 559-575.Qanbari S, Simianer H, 2014. Mapping signatures of positive selection in the genome of livestock. Livest Sci 166: 133-143.R Core Team, 2019. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https: //www. R-project.org/.Rohrer GA, Nonneman DJ, Wiedmann RT, Schneider JF, 2015. A study of vertebra number in pigs confirms the association of vertnin and reveals additional QTL. BMC Genet 16: 129.Sargolzaei M, Chesnais JP, Schenkel FS, 2014. A new approach for efficient genotype imputation using information from relatives. BMC Genom 15: 478.Schneider JF, Miles JR, Brown-Brandl TM, Nienaber JA, Rohrer GA, Vallet JL, 2015. Genomewide association analysis for average birth interval and stillbirth in swine. J Anim Sci 93: 529-540.Sherwood NM, Krueckl SL, McRory JE, 2000. The origin and function of the pituitary adenylate cyclase-activating polypeptide (PACAP)/glucagon superfamily. Endocr Rev 21: 619-670.Silió L, Barragan C, Fernández AI, García-Casco J, Rodríguez MC, 2016. Assessing effective population size, coancestry and inbreeding effects on litter size using the pedigree and SNP data in closed lines of the Iberian pig breed. J Anim Breed Genet 133: 145-154.Smedley D, Haider S, Durinck S, Pandini L, Provero P, Allen J, Arnaiz O, Awedh MH, Baldock R, Barbiera G, et al., 2015. The BioMart community portal: an innovative alternative to large, centralized data repositories. Nucl Acids Res 43: W589-W598.Soilleux EJ, Morris LS, Leslie G, Chehimi J, Luo Q, Levroney E, Trowsdale J, Montaner LJ, Doms RW, Weissman D, Coleman N, Lee B., 2002. Constitutive and induced expression of DC-SIGN on dendritic cell and macrophage subpopulations in situ and in vitro. J Leukoc Biol 71: 445-457.Sterky FH, Trotter JH, Lee S, Recktenwald CV, Du X, Zhou B, Zhou P, Schwenk J, Fakler B, Südhof TC, 2017. Carbonic anhydrase-related protein CA10 is an evolutionary conserved pan-neurexin ligand. Proc Nac Acad Sci 114: E1253-E1262.Vale-Cruz DS, Ma Q, Syme J, LuValle PA, 2008. Activating transcription factor-2 affects skeletal growth by modulating pRb gene expression. Mech Dev 125: 843-856.Velardo LL, Silva FF, Lopes MS, Madsen O, Bastiaansen JW, Knol EF, Kelly M, Varona L, Lopes PS, Guimaräes SEF. 2016. Revealing new candidate genes for reproductive traits in pigs: combining Bayesian GWAS and functional pathways. Genet Sel Evol 48: 9.Ventanas S, Ventanas J, Ruiz J, Estévez M, 2005. Iberian pigs for the development of high-quality cured products. In: Recent Res Devel Agricultural & Food Chem; SG Pandalai (Ed.) 6: 27-53.Wang J, Vasaikar S, Shi Z, Greer M, Zhang B, 2017. WebGestalt 2017: a more comprehensive, powerful, flexible and interactive gene set enrichment analysis toolkit. Nucl Acids Res 45: W130-W137.Weir WS, Cockerham CC, 1984. Estimating F-Statistics for the analysis of population structure. Evolution 38: 1358-1370.Wright S, 1951. The genetical structure of populations. Ann Eugenics 15: 323-354.Wu B, Gong J, Yuan S, Zhang Y, Wei T, 2013. Patterns of evolutionary selection pressure in the immune signaling protein TRAF3IP2 in mammals. Gene 531: 403-410.Yagi T, Shigetani Y, Furuta Y, Nada S, Okado N, Ikawa Y, Aizawa S, 1994. Fyn expression during early neurogenesis in mouse embryos. Oncogene 9: 2433-2440.Yong Y, Meng Y, Ding H, Fan Z, Tang Y, Zhou C, Luo J, Ke ZJ, 2015. PACT/RAX regulates the migration of cerebellar granule neurons in the developing cerebellum. Sci Rep 5: 7961.Zhang F, Zhang Z, Yan X, Chen H, Zhang W, Hong Y, Huang L, 2014. Genome-wide association studies for hematological traits in Chinese Sutai pigs. BMC Genet 15:41

Physicochemical Characterization and Sugar Profile of Argentinian Honeys From the Phytogeographic Provinces Paranaense and Pampeana in Misiones

The characterization of honeys from the Biomes and vegetation units of the Phytogeographical Provinces (PP) located in the Province of Misiones (Argentina) was performed by analysis of sugar profile and physicochemical parameters (Free Acidity, pH, Electrical Conductivity (EC), colour and contents of moisture, ash, total soluble solids and hydroxymethylfurfural) used for quality control of honey. The honeys analysed were authentic and traceable, and met the specifications of the national and/or international standards for the evaluated parameters, denoting blossom origin and confirming high quality, maturity and freshness. The influence of biome and Vegetation Units (VU) on physical and chemical parameters of the studied honeys was defined regarding both flora and pedoclimatic conditions. Honeys from VU-27 presented significantly higher contents of fructose, ash and total soluble solids, pH and EC and lower moisture content than those from VU-3. To the authors’ best knowledge, sugar profiles, free acidity and contents of ash and total soluble solids in honeys from the different PPs, vegetation units and Biomes of Misiones are reported for the first time. This study contributes to the classification of honeys from Misiones, helping to increase added value and access new markets.Fil: Poliero, Aimará Ayelen. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Aubone, Ines. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Amadei Enghelmayer, Marisa. No especifíca;Fil: Rosso, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Müller, P. F.. No especifíca;Fil: Fuselli, Sandra Rosa. Universidad Nacional de Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Producción, Sanidad y Ambiente; ArgentinaFil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentin

Study of use of internet network for Wi-Fi in hospitality in Porto Alegre

A explosão do turismo proporcionou uma expansão espetacular das empresas hoteleiras, o que tem exigido um acompanhamento e aperfeiçoamento dos avanços tecnológicos, com vistas a uma adaptação e capacitação para atender às novas exigências dos usuários modernos. Estes têm a informatização não apenas como uma ferramenta de trabalho ou de lazer, mas sim incorporada à vida pessoal em função do amplo crescimento de novas tecnologias. Em 2014, será realizada, em Porto Alegre, a Copa do Mundo, grande evento com grande fluxo de diferentes perfis de usuários, conectados aos mais diversos tipos de tecnologiasexistentes disponíveis com alta capacidade para trocas de grandes fluxos de informações para os diferentes países. Este artigo tem o objetivo de analisarcomo os empreendimentos hoteleiros estão preparados em relação à capacidadeda rede de Wireless (WI-FI) em todas as suas instalações para atender à demandaque será necessária neste período.The tourism boom has provided a spectacular expansion of the hotel industry, which has required monitoring and improvement of technological advances, with a view to adapting and training to meet the changing needs of modern users. These people have computerization not only as a tool for work or leisure, but they have it incorporated into their personal lives due to the extensive growth of new technologies. In 2014, the World Cup will be held in Porto Alegre, a great event with great flow of different user profiles connected to most types of existing technologies available with high capacity for exchanging large flows of information with different countries . This article aims to analyze how hotels are prepared in relation to the capacity of wireless network (WI-FI) in all their facilities to meet the demand that will be required during this period

Pregnant women's perspectives about maternal immunization in Latin America

Background: Maternal immunization rates and vaccine uptake in Latin America vary from country to country. This variability stems from factors related to pregnant women, vaccine recommendations from healthcare providers and the health system. The aim of this paper is to describe women's knowledge and attitudes to maternal immunziation, and barriers to access and vaccination related decision-making processes in Latin American countries. Methods: We conducted focus group discussions (FGD) with pregnant women in five middle-income countries: Argentina, Brazil, Honduras, Mexico and Peru, between July 2016 and July 2018. The FGDs were conducted by trained qualitative researchers in diverse clinics located in the capital cities of these countries. Results: A total of 162 pregnant women participated in the FGDs. In general, participants were aware of the recommendation to receive vaccines during pregnancy but lacked knowledge regarding the diseases prevented by these vaccines. Pregnant women expressed a desire for clearer and more detailed communication on maternal vaccines by their healthcare professionals instead of relying on other sources of information such as the internet. Overall, participants had positive attitudes towards maternal immunization and were open to receiving vaccines in pregnancy based on general trust they have in recommendations made by their healthcare providers. The main obstacles pregnant women said they encounter were mainly centered around their clinical experience: long waiting times, vaccine shortages, and impolite behavior of healthcare providers or clinical staff. Conclusion: Important advances have been made in Latin America to promote maternal immunization. Results from this study show that an important aspect that remains to be addressed, and is crucial in improving vaccine uptake in pregnancy, is women's clinical experience. We recommend pregnant women to be treated as a priority population for providing immunization and related healthcare education. It is imperative to train healthcare providers in health communication so they can effectively communicate with pregnant women regarding maternal vaccines and can fill knowledge gaps that otherwise might be covered by unreliable sources dispensing inaccurate information.Fil: Fauzia Malik, A.. University of Yale. School of Medicine; Estados UnidosFil: Belizan, María. Instituto de Efectividad Clínica y Sanitaria; ArgentinaFil: Gutierrez, Mariana. University of Emory; Estados UnidosFil: Vilajeliu, Alba. Pan American Health Organization; Estados UnidosFil: Sanclemente, Lauren N.. University of Emory; Estados UnidosFil: Gonzalez Casanova, Ines. Indiana University; Estados Unidos. University of Emory; Estados UnidosFil: Jones, Daniel Eduardo. Instituto de Efectividad Clínica y Sanitaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Omer, Saad. University of Yale; Estados Unidos. University of Yale. School of Medicine; Estados UnidosFil: Maria Ropero, Alba. Pan American Health Organization; Estados UnidosFil: Alonso, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Efectividad Clínica y Sanitaria; Argentin

Chronic hepatitis b and chronic hepatitis C immunopathogenesis: similar but not the same

HBV and HCV are hepatotropic viruses which differ in the way they induce chronic disease. We aimed to compare the hepatic immune response in Chronic Hepatitis B (CHB) and C (CHC) infections and assess their role in liver damage. Immunostaining was done in 68 formalin-fixed and paraffin-embedded liver biopsies from 26 CHB and 42 CHC treatment-naive patients to characterize liver infiltrate: [Th (CD4+), Th1 (Tbet+), Th17 (IL-17A+), Treg (Foxp3+), and CTL (CD8+)]. Quantification: portal (P)= +/total lymphocytes or lobular= + lymphocytes in 10 fields; (400x). Hepatitis severity and fibrosis were assessed by the modified Knodell (HAI) and METAVIR. Comparing CHB and CHC lymphocyte prevalence was alike in P areas (Th>CTL>Treg>Th17>Th1). However, CHC patients showed higher frequencies of Treg, Th17 and Th1 cells (p=0.001, p=0.005 and p=0.003, respectively, U-test). In contrast, cell distribution was different in the lobular area (CHB: CTL> Treg>Th17=Th1>Th vs CHC: CTL>Th1>Treg>Th=Th17) with higher frequencies of Th, Th17 and Th1 cells in CHC (p=0.04, p=0.001 and p=0.001, respectively, U-test) compared with CHB. Regarding liver damage, patients with analogous disease stage showed similar cell frequencies but only in CHC P Th17 were associated with advanced fibrosis (p=0.03, U-test) and just in CHB P Th (p=0.04, U-test) and lobular CTLs and Th17 cells (p=0.02 and p=0.01, respectively; U-test) were increased in severe hepatitis cases.Even when all studied populations were identified in CHB and CHC, common and particular features related to liver damage were detected. Lobular CTLs prevalence in both infections implies their contribution in hepatitis pathogenesis. As for CHB, despite the presence of a regulatory microenvironment, CTLs and Th17 cells promote hepatitis severity, suggesting a Treg failure in limiting liver damage but favouring viral persistence. By contrast, CHC showed a highly inflammatory context with CTL and Th1 majority and Th17 cells enhancing liver fibrosis.Fil: Giadans, Cecilia Graciela. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Rios, Daniela Alejandra. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Cairoli, Victoria. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Ameigeiras, Beatriz. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; ArgentinaFil: Alonso, Ines. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; ArgentinaFil: Pietrantonio, Adriana M.. Gobierno de la Ciudad de Buenos Aires. Hospital General de Agudos "Ramos Mejía"; ArgentinaFil: Mullen, Eduardo. Hospital Italiano; ArgentinaFil: Heinrich, Fabiana. No especifíca;Fil: de Matteo, Elena Noemí. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Valva, Pamela. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaFil: Preciado, Maria Victoria. Gobierno de la Ciudad de Buenos Aires. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto Multidisciplinario de Investigaciones en Patologías Pediátricas; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión Anual de la Sociedad Argentina de Inmunología y Reunión Anual de la Sociedad Argentina FisiologíaArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina Fisiologí