Increased anxiety in corticotropin-releasing factor type 2 receptor-null mice requires recent acute stress exposure and is associated with dysregulated serotonergic activity in limbic brain areas

BACKGROUND: Corticotropin-releasing factor type 2 receptors (CRFR2) are suggested to facilitate successful recovery from stress to maintain mental health. They are abundant in the midbrain raphe nuclei, where they regulate serotonergic neuronal activity and have been demonstrated to mediate behavioural consequences of stress. Here, we describe behavioural and serotonergic responses consistent with maladaptive recovery from stressful challenge in CRFR2-null mice. RESULTS: CRFR2-null mice showed similar anxiety levels to control mice before and immediately after acute restraint stress, and also after cessation of chronic stress. However, they showed increased anxiety by 24 hours after restraint, whether or not they had been chronically stressed. Serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) contents were quantified and the level of 5-HIAA in the caudal dorsal raphe nucleus (DRN) was increased under basal conditions in CRFR2-null mice, indicating increased 5-HT turnover. Twenty-four hours following restraint, 5-HIAA was decreased only in CRFR2-null mice, suggesting that they had not fully recovered from the challenge. In efferent limbic structures, CRFR2-null mice showed lower levels of basal 5-HT in the lateral septum and subiculum, and again showed a differential response to restraint stress from controls. Local cerebral glucose utilization (LCMRglu) revealed decreased neuronal activity in the DRN of CRFR2-null mice under basal conditions. Following 5-HT receptor agonist challenge, LCMRglu responses indicated that 5-HT(1A) receptor responses in the DRN were attenuated in CRFR2-null mice. However, postsynaptic 5-HT receptor responses in forebrain regions were intact. CONCLUSIONS: These results suggest that CRFR2 are required for proper functionality of 5-HT(1A) receptors in the raphe nuclei, and are key to successful recovery from stress. This disrupted serotonergic function in CRFR2-null mice likely contributes to their stress-sensitive phenotype. The 5-HT content in lateral septum and subiculum was notably altered. These areas are important for anxiety, and are also implicated in reward and the pathophysiology of addiction. The role of CRFR2 in stress-related psychopathologies deserves further consideration

Chronic Activation of Corticotropin-Releasing Factor Type 2 Receptors Reveals a Key Role for 5-HT1A Receptor Responsiveness in Mediating Behavioral and Serotonergic Responses to Stressful Challenge

BackgroundThe corticotropin-releasing factor type 2 receptor (CRFR2) is suggested to play an important role in aiding recovery from acute stress, but any chronic effects of CRFR2 activation are unknown. CRFR2 in the midbrain raphé nuclei modulate serotonergic activity of this key source of serotonin (5-HT) forebrain innervation.MethodsTransgenic mice overexpressing the highly specific CRFR2 ligand urocortin 3 (UCN3OE) were analyzed for stress-related behaviors and hypothalamic-pituitary-adrenal axis responses. Responses to 5-HT receptor agonist challenge were assessed by local cerebral glucose utilization, while 5-HT and 5-hydroxyindoleacetic acid content were quantified in limbic brain regions.ResultsMice overexpressing urocortin 3 exhibited increased stress-related behaviors under basal conditions and impaired retention of spatial memory compared with control mice. Following acute stress, unlike control mice, they exhibited no further increase in these stress-related behaviors and showed an attenuated adrenocorticotropic hormone response. 5-HT and 5-hydroxyindoleacetic acid content of limbic nuclei were differentially regulated by stress in UCN3OE mice as compared with control mice. Responses to 5-HT type 1A receptor challenge were significantly and specifically reduced in UCN3OE mice. The distribution pattern of local cerebral glucose utilization and 5-HT type 1A receptor messenger RNA expression levels suggested this effect was mediated in the raphé nuclei.ConclusionsChronic activation of CRFR2 promotes an anxiety-like state, yet with attenuated behavioral and hypothalamic-pituitary-adrenal axis responses to stress. This is reminiscent of stress-related atypical psychiatric syndromes such as posttraumatic stress disorder, chronic fatigue, and chronic pain states. This new understanding indicates CRFR2 antagonism as a potential novel therapeutic target for such disorders

Analytical Derivation of Electrical-Side Maximum Power Line for Wind Generators

In order to enhance the maximum power point tracking (MPPT) speed of solar generators, offline calculated maximum power line (MPL) is often used as a feed-forward signal added to the output of MPPT controller. MPL is nonlinear static electrical characteristic of renewable energy generators connecting all the maximum power points for given temperature. In this letter, electrical side MPL is derived for a typical wind turbine generator (WTG). It is shown that MPLs of solar and wind generators possess similar structure, supporting the similarity between the two energy conversion processes

Quadrature Demodulator-Assisted Estimation of Load Voltage and Resistance Based on Primary-Side Information of a Wireless Power Transfer Link

This paper proposes an algorithm for the extraction of primary-side first harmonic voltage and current components for inductive wireless power transfer (WPT) links by employing quadrature demodulation. Such information allows for the accurate estimation of corresponding receiver-side components and hence permits the monitoring of the output voltage and resistance necessary for protection and/or control without using either sensors or feedback communication. It is shown that precision estimation is held as long as the parameter values of the system are known and the phasor-domain equivalent circuit is valid (i.e., in continuous conduction mode). On the other hand, upon light load operation (i.e., in discontinuous conduction mode), the proposed technique may still be employed if suitable nonlinear correction is employed. The methodology is applied to a 400 V, 1 kW inductive WPT link operating at a load-independent-voltage-output frequency and is well-verified both by simulations and experiments

Maximum perturbation step size in MPP-Tracking control for ensuring predicted PV power settling behavior

The heuristic perturb-and-observe-based maximum-power-point tracking (MPPT) algorithm of photovoltaic (PV) generator is still the most popular technique in use, despite the broad spectrum of developed other MPPT algorithms. The correct direction of the next perturbation step requires that the previous perturbation is settled down properly and the applied perturbation step size is large enough to overcome the PV-power changes induced by the varying irradiation level and/or the power-grid-originated PV-voltage ripple. The requirements for the minimum perturbation step size are well defined in the available literature. The design equations to predict the PV-power settling time are derived by assuming that the PV-interfacing converter operates in continuous conduction mode (CCM). A large perturbation step size may drive the interfacing converter to enter into discontinuous conduction mode (DCM), which will delay the PV-power settling process and destroy the validity of the predicted settling times. In order to avoid confusing the MPPT process, the maximum perturbation step size has to be limited as well. This paper provides theoretical foundations for the proper design of the maximum step size based on the DC-DC interfacing-converter dynamic behavior. The theoretical findings are validated with experiments as well as by simulations by means of a boost-type DC-DC converter and real PV panel.publishedVersionPeer reviewe

Battery-ultracapacitor hybrids for pulsed current loads: A review

Battery versus hybrid power sources performance is examined in the manuscript. Passive, semi-active and fully active battery-ultracapacitor hybrids show obvious superiority over battery only powered pulsed current loads. Passive hybrid is the most simple and cheap arrangement, however its uncontrolled nature results in several drawbacks during the operation. On the other hand, the fully active hybrids achieve superior performance at the expense of two DC-DC converters and the corresponding control circuitry. The trade off between the topologies is the semi-active hybrid, employing only one DC-DC converter and attaining a compromising performance. The thorough characterization of each topology and sub-topology is presented in the manuscript and design methodology is derived for a particular case of pulsed current load.Hybrid sources Power electronics Battery Ultracapacitor

Direct Fixed-Step Maximum Power Point Tracking Algorithms with Adaptive Perturbation Frequency

Owing to the good trade-off between implementation and performance, fixed-step direct maximum power point tracking techniques (e.g., perturb and observe and incremental conductance algorithms) have gained popularity over the years. In order to optimize their performance, perturbation frequency and perturbation step size are usually determined a priori. While the first mentioned design parameter is typically dictated by the worst-case settling time of the combined energy conversion system, the latter must be high enough to both differentiate the system response from that caused by irradiation variation and match the finite resolution of the analog-to-digital converter in case of digital implementation. Well-established design guidelines, however, aim to optimize steady-state algorithm performance while leaving transients nearly untreated. To improve transient behavior while keeping the steady-state operation unaltered, variable step direct maximum power point tracking algorithms based on adaptive perturbation step size were proposed. This paper proposes a concept of utilizing adaptive perturbation frequency rather than variable step size, based on recently revised guidelines for designing fixed-step direct maximum power point tracking techniques. Preliminary results demonstrate the superiority of the proposed method over adaptive perturbation step size operation during transients, without compromising the steady state performance

Comprehensive modeling and formulation of split DC link capacitors balancing problem in Three-Phase Three-Level bidirectional AC/DC converters operating with arbitrary power factor

Three-phase three-level AC/DC and DC/AC converters have demonstrated several advantages over their classical two-level counterparts such as better efficiency, enhanced total harmonic distortion, lower dv/dt and lesser overall chip area. T-type and neutral point clamped (NPC) topologies dominate the family of three-phase three-level converters, widely used in 10kVA – 100kVA power range. However, control of three-level converters is more complicated than of their classical two-level counterparts due to necessity of adopting an additional voltage loop, aimed to balance partial split DC link voltages. Moreover, the latter contain multiples of triple-base-frequency harmonic ripple even under balanced AC-side operation (this does not happen in two-level converters), which should be minimized by the balancing loop mentioned above. The nature of partial DC link ripple formation was analyzed in-depth for unity power factor operation, yet hardly extended to non-unity power factor cases. Moreover, inaccurate conclusions were drawn in some of the related studies. Consequently, this paper focuses on comprehensive modeling and formulation of split DC link capacitors balancing problem in three-phase three-level bidirectional AC/DC converters operating with arbitrary power factor. Analytical expression for dynamics of partial DC link voltages difference is derived and studied in depth. The relation between zero-sequence component of modulation signals and neutral point current, tending to unbalance partial DC link voltages is explicitly established for arbitrary power factor values. Practical cases of restricted and unrestricted zero-sequence component of modulation signals are presented. Analytical predictions are shown to be fully supported by simulations based on both switching-cycle-averaged and full switched converter models. Experimental results obtained employing a 10kVA T-type converter prototype operating with restricted zero-sequence component of modulation signals under different power factor values are also given to further validate the presented methodology