Genomic Exclusion in Tetrahymena: Genetic Basis *

Genomic exclusion is an aberration that occurs during conjugation in variety 1 of Tetrahymena pyriformis. Instead of containing markers from both parents, the out cross pairs are either homozygous for all the genes of one parent (unilateral genomic exclusion); or, some of the pairs are homozygous for the genes of one parent and other pairs are homozygous for the genes of the other parent (bilateral genomic exclusion). This phenomenon was first demonstrated in the C strain: some stocks evoke unilateral genomic exclusion; others, bilateral genomic exclusion. C*, inbred for 5 generations, was used to explore this phenomenon in some detail since unilateral genomic exclusion of C genes occurs in almost all pairs in outcrosses of C*. In a mating of C*, both exconjugants are recovered, both are dipioid and similar in phenotype. Using morphological markers, C* can be shown to participate in the mating; therefore, C* does not induce illegitimate matings of the normal mate. When the normal mate is heterozygous for alleles ( H A / H D ) not present in C*, 3 classes of offspring ( H A / H A , H A / H D and H D / H D ) are produced in a 1:2:1 ratio. These observations indicate that 2 meiotic products of the normal mate unite to form the syn carya. The genetic ratios obtained in 1 and 2 factor crosses limit the possible cytogenetic bases for genomic exclusion. They suggest that 1 of the 4 haploid nuclei replicates and the replica fuses randomly with any 1 of the 4 nuclei. The 2 schemes of nuclear behavior (single fertilization, double fertilization) that would satisfy these requirements have not yet been resolved.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73138/1/j.1550-7408.1963.tb01699.x.pd

GENETIC CONTROL OF THE ESTERASES IN THE PROTOZOAN TETRAHYMENA PYRIFORMIS *

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72830/1/j.1749-6632.1961.tb35571.x.pd

Documentation of the current fault detection, isolation and reconfiguration software of the AIPS fault-tolerant processor

Documentation is presented of the December 1986 version of the ADA code for the fault detection, isolation, and reconfiguration (FDIR) functions of the Advanced Information processing System (AIPS) Fault-Tolerant Processor (FTP). Because the FTP is still under development and the software is constantly undergoing changes, this should not be considered final documentation of the FDIR software of the FTP

Women coaches' perceptions of their sport organizations' social environment: Supporting coaches' psychological needs?

Researchers have argued that coaches are performers in their own right and that their psychological needs should be considered (Giges, Petitpas, & Vernacchia, 2004; Gould, Greenleaf, Guinan, & Chung, 2002). The purpose of this research was to examine high performance women coaches’ perceptions of their sport organizations’ social context, with specific attention to psychological need support. Self-Determination Theory (Deci & Ryan, 1985; Ryan & Deci, 2002) was employed to frame the examination of the coaches’ experiences. Eight high performance women coaches from two sport organizations participated in semistructured interviews. All reported autonomy and competence development opportunities. Organizational relatedness was critical to the experience of a supportive environment. The findings provide insight into the "world of coaching" from the coaches’ perspective

An Interdisciplinary Approach to Examining the Working Conditions of Women Coaches

An interdisciplinary framework allowed psychological and sociological approaches to be combined to examine coaches' working conditions, specifically to examine the structural aspects and social values within the sport organisations as well as the implications for individual aspects. Ten participants from two sport organisations took part in the study. They were eight women performance coaches and two coach managers. Participants were interviewed to explore their perceptions of the working environment in their sport organisation including involvement, support, opportunities, and relationships. Four themes were developed characterising coaches' perceptions of their working conditions. These were relationships with key organisational personnel, coach as a person, learning and development opportunities, and relationships among coaches. The themes were examined both within and between the two sport organisations. The structures and values of one organisation fostered working conditions conducive to the need satisfaction of the coaches. The working conditions within the second organisation were less effective, but reflected its organisational values. The findings are discussed in relation to coaching research and provide an alternative approach to examining the social context of coaching

What Couples Say Works in Domestic Violence Therapy

This ethnographic study adds to our professional knowledge about the effectiveness of domestic violence counseling. We learned from couples who had been court referred for domestic violence counseling what they found to be most effective in their therapy. The study was conducted by a student practitioner-researcher. The inquiry is written in an open manner and details the procedural steps, discusses trustworthiness specific to the inquiry, and includes reflections from the authors

Effect of Acetate on Esterase C Activity During the Growth Cycle of Paramecium *

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/100130/1/j.1550-7408.1978.tb04415.x.pd

Macronuclear persistence of sequences normally eliminated during development in Tetrahymena thermophila

During conjugation in the ciliated protozoan, Tetrahymena thermophila , a somatic MAC-ronucleus develops from the germinal MICronucleus. Ten to 20 percent of the MIC genome is eliminated during this process. Three repetitive families have been identified which have different levels of repetition in the MIC and are eliminated to different degrees in the MAC. Some members of two of these families persist in the MAC. In this study, we have looked at these persistent sequences in the MAC of cell lines from a variety of sources including several inbed strains, two sets of caryonides, caryonidal subclones, and vegetatively aged cell clones. The results suggest that the sequences that remain in the MAC have a genetic predisposition to persist. However, epigenetic variations occur as the MAC develops so that only some of the persistent sequences are actually observed in a particular MAC. Polymorphisms may be generated if alternative processing of a single MIC segment occurs. These polymorphisms can later be resolved by phenotypic assortment during vegetative growth. These facultatively persistent sequences appear to differ from sequences previously described in this organism.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50176/1/1020060205_ftp.pd

S6E10: How has UMaine impacted Allen and Sally Fernald and vice versa?

Allen and Sally Fernald of Camden, Maine have long been advocates for the University of Maine. Since graduating in 1954 and 1955, the owners and publishers of Down East Magazine have donated to various capital campaigns, sponsored art galleries and poetry readings, and led alumni groups and fundraising drives. In this week’s episode of “The Maine Question,” the Fernalds discuss their lifelong relationship with their alma mater, and how the state and UMaine have evolved overtime, including the university’s development into a top-tier, nationally recognized research institution