Correlated responses in lines of Drosophila melanogaster selected for different oviposition behaviours.

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Probing DNA conformational changes with high temporal resolution by Tethered Particle Motion

The Tethered Particle Motion (TPM) technique informs about conformational changes of DNA molecules, e.g. upon looping or interaction with proteins, by tracking the Brownian motion of a particle probe tethered to a surface by a single DNA molecule and detecting changes of its amplitude of movement. We discuss in this context the time resolution of TPM, which strongly depends on the particle-DNA complex relaxation time, i.e. the characteristic time it takes to explore its configuration space by diffusion. By comparing theory, simulations and experiments, we propose a calibration of TPM at the dynamical level: we analyze how the relaxation time grows with both DNA contour length (from 401 to 2080 base pairs) and particle radius (from 20 to 150~nm). Notably we demonstrate that, for a particle of radius 20~nm or less, the hydrodynamic friction induced by the particle and the surface does not significantly slow down the DNA. This enables us to determine the optimal time resolution of TPM in distinct experimental contexts which can be as short as 20~ms.Comment: Improved version, to appear in Physical Biology. 10 pages + 10 pages of supporting materia

Entrainment and deposition of boulders in a gravel bed river

Bedload transport, entrainment of coarse sediment by a river, is inherently a stochastic and intermittent process whose monitoring remains challenging. Here, we propose a new method to characterize bedload transport in the field. Using an uncrewed aerial vehicle (UAV) equipped with a high-resolution camera, we recorded yearly images of a bar of the Grande Rivière des Vieux-Habitants, a gravel bed river located on Basse-Terre Island (Guadeloupe, French West Indies). These images, combined with high-frequency measurements of the river discharge, allow us to monitor the evolution of the population of sediments of a diameter between 0.5 and 0.75 m on the riverbed. Based on this dataset, we estimate the smallest discharge that can move these boulders and calculate the duration of effective transport. We find that the transport of boulders occurs for approximately 10 h yr−1. When plotted as a function of the effective transport time, a given population of boulders decreases exponentially with an effective residence time of approximately 17 h. This exponential decay suggests that the probability of dislodging a grain from the bed is proportional to the number of grains at repose on the bed, an observation consistent with laboratory experiments. Finally, the residence time of bedload particles on a riverbed can be used to evaluate bedload discharge.</p

Genetic properties of North African Drosophila melanogaster and comparison with European and Afrotropical populations

International audienc

Decoding the origins of vertical land motions observed today at coasts

In recent decades, geodetic techniques have allowed detecting vertical land motions and sea-level changes of a few millimetres per year, based on measurements taken at the coast (tide gauges), on board of satellite platforms (satellite altimetry) or both (Global Navigation Satellite System). Here, contemporary vertical land motions are analysed from January 1993 to July 2013 at 849 globally distributed coastal sites. The vertical displacement of the coastal platform due to surface mass changes is modelled using elastic and viscoelastic Green's functions. Special attention is paid to the effects of glacial isostatic adjustment induced by past and present-day ice melting. Various rheological and loading parameters are explored to provide a set of scenarios that could explain the coastal observations of vertical land motions globally. In well-instrumented regions, predicted vertical land motions explain more than 80 per cent of the variance observed at scales larger than a few hundred kilometres. Residual vertical land motions show a strong local variability, especially in the vicinity of plate boundaries due to the earthquake cycle. Significant residual signals are also observed at scales of a few hundred kilometres over nine well-instrumented regions forming observation windows on unmodelled geophysical processes. This study highlights the potential of our multitechnique database to detect geodynamical processes, driven by anthropogenic influence, surface mass changes (surface loading and glacial isostatic adjustment) and tectonic activity (including the earthquake cycle, sediment and volcanic loading, as well as regional tectonic constraints). Future improvements should be aimed at densifying the instrumental network and at investigating more thoroughly the uncertainties associated with glacial isostatic adjustment models.This research benefited from financial support from the CNES (Centre National d’Etudes Spatiales, France) through the TOSCA committee fellowship and from the European Research Council within the framework of the SP2-Ideas Program ERC-2013-CoG, under ERC Grant agreement number 617588. GS is supported by a DiSPeA research grant (CUP H32I160000000005) and by Programma Nazionale di Ricerche in Antartide (PNRA 2013/B2.06, CUP D32I14000230005). AM was supported by an Australian Research Council Super Science Fellowship (FS110200045)

High speed videometric monitoring of rock breakage

Estimation of rock breakage characteristics plays an important role in optimising various industrial and mining processes used for rock comminution. Although little research has been undertaken into 3D photogrammetric measurement of the progeny kinematics, there is promising potential to improve the efficacy of rock breakage characterisation. In this study, the observation of progeny kinematics was conducted using a high speed, stereo videometric system based on laboratory experiments with a drop weight impact testing system. By manually tracking individual progeny through the captured video sequences, observed progeny coordinates can be used to determine 3D trajectories and velocities, supporting the idea that high speed video can be used for rock breakage characterisation purposes. An analysis of the results showed that the high speed videometric system successfully observed progeny trajectories and showed clear projection of the progeny away from the impact location. Velocities of the progeny could also be determined based on the trajectories and the video frame rate. These results were obtained despite the limitations of the photogrammetric system and experiment processes observed in this study. Accordingly there is sufficient evidence to conclude that high speed videometric systems are capable of observing progeny kinematics from drop weight impact tests. With further optimisation of the systems and processes used, there is potential for improving the efficacy of rock breakage characterisation from measurements with high speed videometric systems

Towards improved socio-economic assessments of ocean acidification’s impacts

Ocean acidification is increasingly recognized as a component of global change that could have a wide range of impacts on marine organisms, the ecosystems they live in, and the goods and services they provide humankind. Assessment of these potential socio-economic impacts requires integrated efforts between biologists, chemists, oceanographers, economists and social scientists. But because ocean acidification is a new research area, significant knowledge gaps are preventing economists from estimating its welfare impacts. For instance, economic data on the impact of ocean acidification on significant markets such as fisheries, aquaculture and tourism are very limited (if not non-existent), and non-market valuation studies on this topic are not yet available. Our paper summarizes the current understanding of future OA impacts and sets out what further information is required for economists to assess socio-economic impacts of ocean acidification. Our aim is to provide clear directions for multidisciplinary collaborative research

Mechanism of strand displacement synthesis by DNA replicative polymerases

Replicative holoenzymes exhibit rapid and processive primer extension DNA synthesis, but inefficient strand displacement DNA synthesis. We investigated the bacteriophage T4 and T7 holoenzymes primer extension activity and strand displacement activity on a DNA hairpin substrate manipulated by a magnetic trap. Holoenzyme primer extension activity is moderately hindered by the applied force. In contrast, the strand displacement activity is strongly stimulated by the applied force; DNA polymerization is favoured at high force, while a processive exonuclease activity is triggered at low force. We propose that the DNA fork upstream of the holoenzyme generates a regression pressure which inhibits the polymerization-driven forward motion of the holoenzyme. The inhibition is generated by the distortion of the template strand within the polymerization active site thereby shifting the equilibrium to a DNA-protein exonuclease conformation. We conclude that stalling of the holoenzyme induced by the fork regression pressure is the basis for the inefficient strand displacement synthesis characteristic of replicative polymerases. The resulting processive exonuclease activity may be relevant in replisome disassembly to reset a stalled replication fork to a symmetrical situation. Our findings offer interesting applications for single-molecule DNA sequencing