Two-phase stretching of molecular chains

While stretching of most polymer chains leads to rather featureless force-extension diagrams, some, notably DNA, exhibit non-trivial behavior with a distinct plateau region. Here we propose a unified theory that connects force-extension characteristics of the polymer chain with the convexity properties of the extension energy profile of its individual monomer subunits. Namely, if the effective monomer deformation energy as a function of its extension has a non-convex (concave up) region, the stretched polymer chain separates into two phases: the weakly and strongly stretched monomers. Simplified planar and 3D polymer models are used to illustrate the basic principles of the proposed model. Specifically, we show rigorously that when the secondary structure of a polymer is mostly due to weak non-covalent interactions, the stretching is two-phase, and the force-stretching diagram has the characteristic plateau. We then use realistic coarse-grained models to confirm the main findings and make direct connection to the microscopic structure of the monomers. We demostrate in detail how the two-phase scenario is realized in the \alpha-helix, and in DNA double helix. The predicted plateau parameters are consistent with single molecules experiments. Detailed analysis of DNA stretching demonstrates that breaking of Watson-Crick bonds is not necessary for the existence of the plateau, although some of the bonds do break as the double-helix extends at room temperature. The main strengths of the proposed theory are its generality and direct microscopic connection.Comment: 16 pges, 22 figure

Decoding the origins of vertical land motions observed today at coasts

In recent decades, geodetic techniques have allowed detecting vertical land motions and sea-level changes of a few millimetres per year, based on measurements taken at the coast (tide gauges), on board of satellite platforms (satellite altimetry) or both (Global Navigation Satellite System). Here, contemporary vertical land motions are analysed from January 1993 to July 2013 at 849 globally distributed coastal sites. The vertical displacement of the coastal platform due to surface mass changes is modelled using elastic and viscoelastic Green's functions. Special attention is paid to the effects of glacial isostatic adjustment induced by past and present-day ice melting. Various rheological and loading parameters are explored to provide a set of scenarios that could explain the coastal observations of vertical land motions globally. In well-instrumented regions, predicted vertical land motions explain more than 80 per cent of the variance observed at scales larger than a few hundred kilometres. Residual vertical land motions show a strong local variability, especially in the vicinity of plate boundaries due to the earthquake cycle. Significant residual signals are also observed at scales of a few hundred kilometres over nine well-instrumented regions forming observation windows on unmodelled geophysical processes. This study highlights the potential of our multitechnique database to detect geodynamical processes, driven by anthropogenic influence, surface mass changes (surface loading and glacial isostatic adjustment) and tectonic activity (including the earthquake cycle, sediment and volcanic loading, as well as regional tectonic constraints). Future improvements should be aimed at densifying the instrumental network and at investigating more thoroughly the uncertainties associated with glacial isostatic adjustment models.This research benefited from financial support from the CNES (Centre National d’Etudes Spatiales, France) through the TOSCA committee fellowship and from the European Research Council within the framework of the SP2-Ideas Program ERC-2013-CoG, under ERC Grant agreement number 617588. GS is supported by a DiSPeA research grant (CUP H32I160000000005) and by Programma Nazionale di Ricerche in Antartide (PNRA 2013/B2.06, CUP D32I14000230005). AM was supported by an Australian Research Council Super Science Fellowship (FS110200045)

Control of Pre-mRNA Splicing by the General Splicing Factors PUF60 and U2AF65

Pre-mRNA splicing is a crucial step in gene expression, and accurate recognition of splice sites is an essential part of this process. Splice sites with weak matches to the consensus sequences are common, though it is not clear how such sites are efficiently utilized. Using an in vitro splicing-complementation approach, we identified PUF60 as a factor that promotes splicing of an intron with a weak 3' splice-site. PUF60 has homology to U2AF(65), a general splicing factor that facilitates 3' splice-site recognition at the early stages of spliceosome assembly. We demonstrate that PUF60 can functionally substitute for U2AF(65)in vitro, but splicing is strongly stimulated by the presence of both proteins. Reduction of either PUF60 or U2AF(65) in cells alters the splicing pattern of endogenous transcripts, consistent with the idea that regulation of PUF60 and U2AF(65) levels can dictate alternative splicing patterns. Our results indicate that recognition of 3' splice sites involves different U2AF-like molecules, and that modulation of these general splicing factors can have profound effects on splicing

Induced Magnetic Ordering by Proton Irradiation in Graphite

We provide evidence that proton irradiation of energy 2.25 MeV on highly-oriented pyrolytic graphite samples triggers ferro- or ferrimagnetism. Measurements performed with a superconducting quantum interferometer device (SQUID) and magnetic force microscopy (MFM) reveal that the magnetic ordering is stable at room temperature.Comment: 3 Figure

Towards improved socio-economic assessments of ocean acidification’s impacts

Ocean acidification is increasingly recognized as a component of global change that could have a wide range of impacts on marine organisms, the ecosystems they live in, and the goods and services they provide humankind. Assessment of these potential socio-economic impacts requires integrated efforts between biologists, chemists, oceanographers, economists and social scientists. But because ocean acidification is a new research area, significant knowledge gaps are preventing economists from estimating its welfare impacts. For instance, economic data on the impact of ocean acidification on significant markets such as fisheries, aquaculture and tourism are very limited (if not non-existent), and non-market valuation studies on this topic are not yet available. Our paper summarizes the current understanding of future OA impacts and sets out what further information is required for economists to assess socio-economic impacts of ocean acidification. Our aim is to provide clear directions for multidisciplinary collaborative research

FAO/WHO GIFT (Global Individual Food consumption data Tool): a global repository for harmonised individual quantitative food consumption studies

Knowing who eats what, understanding the various eating habits of different population groups, according to the geographical area, is critical to develop evidence-based policies for nutrition and food safety. The FAO/WHO Global Individual Food consumption data Tool (FAO/WHO GIFT) is a novel open-access online platform, hosted by FAO and supported by WHO, providing access to harmonised individual quantitative food consumption (IQFC) data, especially in low- and middle-income countries (LMIC). FAO/WHO GIFT is a growing repository, which will serve as the global FAO/WHO hub to disseminate IQFC microdata. Currently five datasets from LMIC are available for dissemination, and an additional fifty datasets will be made available by 2022. To facilitate the use of these data by policy makers, ready-to-use food-based indicators are provided for an overview of key data according to population segments and food groups. FAO/WHO GIFT also provides an inventory of existing IQFC data worldwide, which currently contains detailed information on 188 surveys conducted in seventy-two countries. In order for end-users to be able to aggregate the available data, all datasets are harmonised with the European Food Safety Authority's food classification and description system FoodEx2 (modified for global use). This harmonisation is aimed at enhancing the consistency and reliability of nutrient intake and dietary exposure assessments. FAO/WHO GIFT is developed in synergy with other global initiatives aimed at increasing the quality, availability and use of IQFC data in LMIC to enable evidence-based decision-making and policy development for better nutrition and food safety

Optimal Operation of the Multireservoir System in the Seine River Basin Using Deterministic and Ensemble Forecasts

International audienceThis article investigates the improvement of the operation of a four-reservoir system in the Seine River basin, France, by use of deterministic and ensemble weather forecasts and real-time control. In the current management, each reservoir is operated independently from the others and following prescribed rule-curves, designed to reduce floods and sustain low flows under the historical hydrological conditions. However, this management system is inefficient when inflows are significantly different from their seasonal average and may become even more inadequate to cope with the predicted increase in extreme events induced by climate change. In this work, a centralized real-time control system is developed to improve reservoirs operation by exploiting numerical weather forecasts that are becoming increasingly available. The proposed management system implements a well-established optimization technique, model predictive control (MPC), and its recently modified version that can incorporate uncertainties, tree-based model predictive control (TB-MPC), to account for deterministic and ensemble forecasts respectively. The management system is assessed by simulation over historical events and compared to the no-forecasts strategy based on rule-curves. Simulation results show that the proposed real-time control system largely outperforms the no-forecasts management strategy, and that explicitly considering forecast uncertainty through ensembles can compensate for the loss in performance due to forecast inaccuracy

Biological status and dietary intakes ofiIron, zinc and vitamin A among women and preschool children in rural Burkina Faso

Background Food-based approaches such as biofortification are meant to sustainably address micronutrient deficiencies in poor settings. Knowing more about micronutrient intakes and deficiencies is a prerequisite to designing and evaluating interventions. Objective The objectives of the study were to assess biological status and dietary intakes of iron, zinc and vitamin A among women and children aged 36-59 months in rural Burkina Faso and to study relationships between intake and status to better inform future food-based interventions. Design A cross-sectional survey was carried out in two rural provinces of Burkina Faso on a random cluster sample of 480 mother-child pairs. Dietary data was obtained by 24-hour recalls repeated on a random sub-selection of 37.5% of subjects to allow calculation of nutrient's probability of adequacy (PA). Biomarkers were measured on a sub-sample of 180 mother-child pairs. Blood samples were analyzed for hemoglobin, serum ferritin, soluble transferrin receptors (sTfR), C-reactive protein, alpha-1-glycoprotein, serum zinc concentration (SZnC) and retinol. For each micronutrient the relationship between biomarker and dietary intake was investigated by multiple linear regression models accounting for inflammatory biomarkers. Results Mean PA for iron, zinc and vitamin A was 0.49, 0.87 and 0.21 among women and 0.61, 0.95 and 0.33 among children, respectively. Prevalence of anemia, corrected low serum ferritin and high sTfR was 37.6%, 4.0% and 77.5% among women and 72.1%, 1.5% and 87.6% among children, respectively. Prevalence of low SZnC and corrected low serum retinol was 39.4% and 12.0% among women and 63.7% and 24.8% among children, respectively. There was a tendency for a positive relationship between vitamin A intakes and serum retinol among women (beta = 0.0003, P = 0.06). Otherwise, no link was found between micronutrients biomarkers and intakes. Conclusion Our study depicted different images of micronutrient deficiencies when based on dietary intakes or biomarkers results, thus highlighting the need for more suitable biomarkers and more precise measures of absorbable micronutrient intakes at the individual level. It thus points to challenges in the design and evaluation of future biofortification or other food-based interventions in rural areas of Burkina Faso