Genetic differentiation and hybridization in two naturally occurring sympatric trout Salmo spp. forms from a small karstic lake.

In this study, multiple molecular markers [genotyping of 12 nuclear microsatellite loci and the protein-coding gene ldh-c1 * plus sequencing of the mitochondrial DNA (mtDNA) control region] were employed to investigate the genetic structure of the two trout forms, Salmo cettii and Salmo fibreni , inhabiting Lake Posta Fibreno, central Italy. The two forms were found to share a unique mtDNA haplotype, belonging to a widespread Mediterranean haplogroup (AD). Bayesian clustering analyses showed that these two forms correspond to well-defined autochthonous gene pools. Genetic introgression between the two gene pools, however, was observed, whose frequency appears to correlate with the environmental features of the spawning sites. The interplay of selection for the spawning sites, philopatry and natural selection can be argued to maintain genetic differentiation despite the lack of complete reproductive isolation

A taxonomic revision of western Eupholidoptera bush crickets (Orthoptera: Tettigoniidae): testing the discrimination power of DNA barcode.

The genus Eupholidoptera includes 46 Mediterranean species distributed from Turkey to Greece, Italy and southern France. In the eastern part of its range, Eupholidoptera has been considered to consist of several distinct species, while in the Balkans and Italian peninsula only E. chabrieri has been recognized. However, the status of some Italian populations, confined to particular geographic areas, remains uncertain. To investigate the delimitation of the Italian taxa of Eupholidoptera, we performed both morphological and molecular analyses. Morphological analysis was carried out by considering diagnostic characters usually used to distinguish different taxa, such as the shape of titillators in males and the subgenital plate in females. Molecular analysis was performed by sequencing three mitochondrial genes: 12S rRNA, 16S rRNA, partially sequenced and the entire gene of cox1 . Molecular markers were used to infer phylogenetic relationships among the Italian Eupholidoptera species and to reconstruct the historical processes that shaped their current geographic distribution. Results from both morphological and molecular analyses were used to revise the taxonomic arrangement of species. On the whole we were able to distinguish nine lineages of Italian Eupholidoptera, of which E. tyrrhenica sp.n. from Corsica is described as a new species

Crack of a helicopter main rotor actuator attachment: failure analysis and lessons learned

A Light Utility Helicopter (LUH), in the course of a training flight, leaving the ground during thetaxi to take off, went into an uncontrolled rolling to the right; consequently the helicopter gradually laid downon the right side. The impact with the runway destroyed the rotating blades up to the hubs rotor. The accidentinvestigation focused on main rotor oscillatory plate servo actuators . These components, directly linked to thecloche movements, regulate main rotor blades plane tilt and pitch. Following the preliminary examination, onlyfront servo actuator attachment was found to be broken in two parts. In detail, the present paper deals with thefracture analysis results. The servo actuator attachment material is a 2014 Aluminum alloy extrudate, undergoneto T651 heat treatment. Fracture surfaces were examined by optical and electronic microscopy in order todetermine the main morphological features and consequently to trace the origin of failure mechanism andcauses. The accordance with the specification requirements about alloy composition was verified by quantitativeelementary analysis through inductive coupled plasma spectroscopy (ICP); furthermore, semi-quantitativeelementary analysis was locally verified by Energy dispersion spectroscopy X ray (EDS_RX). Finally, thehydrogen content of the material was evaluated by the total hydrogen analysis. Microstructural andtechnological alloy characteristics were verified as well by using metallographic microscopy and hardness testingof the material.Macroscopic fracture surfaces evidences were characterized by the lack of any significant plastic deformationsand by the presence of symmetry compared to the servo actuator axis. Microscopic fracture features of both theinvestigated surfaces were not coherent to the hypothesis of an impact of the main rotor to the soil. Furtherachieved evidences, such as grain boundary fracture propagation, the presence of corrosion products, were all inaccordance with a Stress Corrosion Cracking (SCC) progressive fracture mechanism.Finite Element Analysis (FEA) located the highest tensile stress value, when the servo actuator is in its nominalworking condition, at the same points where the corrosion products were more concentrated (i.e. in the part ofthe fracture exposed to oxidative air effect for the longest time). The good agreement between FEA andmorphological evidences allowed to determine the progressive fracture origin area, though it was not possible toindividuate the crack initiation point. In fact, in correspondence to the initiation area of both the fracturesurfaces, shining and flat morphology was found;. then there were evidence of plastic deformations, due to thedetachment of a servo actuator part.The ICP analysis and hardness testing results were in accordance with the material specification requirements.However, the hydrogen content was one order of magnitude greater than the required value and many andunexpected globular formations were observed on the fracture surface. Part of these were dendritic formations,while the others looked smooth and shining. Further, FESEM boundary grain observation gave evidences of ahigh presence of precipitates on the investigated surfaces. Hence, observed microstructural characteristics,boundary grain precipitates and globular formations allowed to hypothesize possible overheating/eutecticmelting phenomena, occurred during manufacturing processes.As widely reported in literature, the AA 2014 alloy is one of the aluminum-copper-magnesium-silicon type,employing copper aluminide (CuAl2 ) as the primary precipitation-hardening agent. The need for a maximumCu phase dispersion in solid solution requires a heat treatment range with an upper limit (507°C) that is near tothe melting of the eutectics (510°C). Moreover, since the 1960s, AA2014 has been defined as sensitive to SCC.This condition is mainly related to the presence of coarse-grained and aligned CuAl2 precipitates. Thisarrangement is due to an overheating (more than 507°C) or to a cooling process carried out too slowly.Microstructural analysis was carried out on three items: 1) a large portion of the broken actuator attachment; 2)on a servoactuator coming from the same production batch; 3) on a servo actuator coming from a differentproduction batch.The microstructure from the broken actuator attachment showed a great amount of precipitates (secondphases) lengthwise aligned to the boundary grain, pores, and also cavities and dendritic globular formations.Analysis results, morphology evidences and reference images available on scientific literature were found to bein excellent agreement and validated the embrittlement and subsequent SCC mechanism hypotesis(intergranular failure propagation).In conclusion, flight accident causes are attributable to main rotor actuator attachment failure.Failure mechanism is classifiable as SCC supported by microstructural anomalies of the material. Theinvestigation of the manufacturing process highlighted how one of the servo actuator batches was not properlyproduced due to poor control and accuracy of heat treatment temperature and/or cooling time. This led tohydrogen embrittlement and to a microstructural problem (globular formations and boundary grainprecipitates). The combination of those phenomena caused an increase of the SCC sensitivity and were thebasic progressive failure driving forces.Nevertheless, as above mentioned, alloy composition was found compliant with the material specificationrequirements and this just because none of the scheduled quality control tests is able to determine the peculiarmicrostructural anomalies reported

The diet of Weddell seals (Leptonychotes weddellii) in Terra Nova Bay using stable isotope analysis

Stable isotope analyses were used to investigate the diet of Weddell seals in Terra Nova Bay (Ross Sea) and the potential variation of their foraging behaviour with age, sex and body mass. For this purpose, skin samples were collected from adult breeding seals and pups, together with muscle samples of their potential prey. Our results showed variation in foraging behavior between age classes, with pups reporting lower δ13C values than adults, while no significant differences in δ15N were recorded. In addition, contrary to expectations, a mixing model analysis showed that adult seals foraged mainly on shallow benthic prey, such as Trematomus spp. (34.1%) and Dissostichus mawsoni (21.1%), rather than on pelagic fish, such as Pleuragramma antarcticum (9.8%). Overall, with this paper we provide novel diet information on a seal colony not previously sampled, adding new insight into the feeding ecology of a top Antarctic predator

Overcoming Drug Resistance and Treating Advanced Prostate Cancer.

Most of the prostate cancers (PCa) in advanced stage will progress to castration-resistant prostate cancer (CRPC). Within CRPC group, 50-70% of the patients will develop bone metastasis in axial and other regions of the skeleton. Once PCa cells spread to the bone, currently, no treatment regimens are available to eradicate the metastasis, and cancer- related death becomes inevitable. In 2012, it is estimated that there will be 28,170 PCa deaths in the United States. Thus, PCa bone metastasis-associated clinical complications and treatment resistance pose major clinical challenges. In this review, we will present recent findings on the molecular and cellular pathways that are responsible for bone metastasis of PCa. We will address several novel mechanisms with a focus on the role of bone and bone marrow microenvironment in promoting PCa metastasis, and will further discuss why prostate cancer cells preferentially metastasize to the bone. Additionally, we will discuss novel roles of several key pathways, including angiogenesis and extracellular matrix remodeling in bone marrow and stem cell niches with their relationship to PCa bone metastasis and poor treatment response. We will evaluate how various chemotherapeutic drugs and radiation therapies may allow aggressive PCa cells to gain advantageous mutations leading to increased survival and rendering the cancer cells to become resistant to treatment. The novel concept relating several key survival and invasion signaling pathways to stem cell niches and treatment resistance will be reviewed. Lastly, we will provide an update of several recently developed novel drug candidates that target metastatic cancer microenvironments or niches, and discuss the advantages and significance provided by such therapeutic approaches in pursuit of overcoming drug resistance and treating advanced PCa

Improved performance of porphyrin-based dye sensitised solar cells by phosphinic acid surface treatment

Chemical surface treatment of porphyrin-sensitised titania films using bis-(4-methoxyphenyl) phosphinic acid after dye adsorption, results in large improvements in DSSC efficiencies which originate primarily from higher short circuit currents. The result was attributed to a positive shift in the TiO2 quasi-Fermi level with simultaneous retardation of charge recombination. High device performances have been achieved even using simplified electrolyte matrices devoid of the common additives, LiI and t-butylpyridine

Axolotl Nanog activity in mouse embryonic stem cells demonstrates that ground state pluripotency is conserved from urodele amphibians to mammals

Cells in the pluripotent ground state can give rise to somatic cells and germ cells, and the acquisition of pluripotency is dependent on the expression of Nanog. Pluripotency is conserved in the primitive ectoderm of embryos from mammals and urodele amphibians, and here we report the isolation of a Nanog ortholog from axolotls (axNanog). axNanog does not contain a tryptophan repeat domain and is expressed as a monomer in the axolotl animal cap. The monomeric form is sufficient to regulate pluripotency in mouse embryonic stem cells, but axNanog dimers are required to rescue LIF-independent self-renewal. Our results show that protein interactions mediated by Nanog dimerization promote proliferation. More importantly, they demonstrate that the mechanisms governing pluripotency are conserved from urodele amphibians to mammals. © 2010. Published by The Company of Biologists Ltd

A 3D Heterotypic Breast Cancer Model Demonstrates a Role for Mesenchymal Stem Cells in Driving a Proliferative and Invasive Phenotype

Previous indirect 2D co-culture studies have demonstrated that mesenchymal stem cells (MSCs) promote breast cancer (BC) progression through secretion of paracrine factors including growth factors, cytokines and chemokines. In order to investigate this aspect of the tumour microenvironment in a more relevant 3D co-culture model, spheroids incorporating breast cancer cells (BCCs), both cell lines and primary BCCs expanded as patient-derived xenografts, and MSCs were established. MSCs in co-cultures were shown to enhance proliferation of estrogen receptor (ER)/progesterone receptor (PR)-positive BCCs. In addition, co-culture resulted in downregulation of E-cadherin in parallel with upregulation of the epithelial-mesenchymal transition (EMT)-relation transcription factor, SNAIL. Cytoplasmic relocalization of ski-related novel protein N (SnON), a negative regulator of transforming growth factor-beta (TGF-β) signalling, and of β-catenin, involved in a number of pathways including Wnt signalling, was also observed in BCCs in co-cultures in contrast to monocultures. In addition, the β-catenin inhibitor, 3-[[(4-methylphenyl)sulfonyl]amino]-benzoic acid methyl ester (MSAB), mediated reduced growth and invasion in the co-cultures. This study highlights the potential role for SnON as a biomarker for BC invasiveness, and the importance of interactions between TGF-β and Wnt signalling, involving SnON. Such pathways may contribute towards identifying possible targets for therapeutic intervention in BC patients