Integrated stress response of Escherichia coli to methylglyoxal : transcriptional readthrough from the nemRA operon enhances protection through increased expression of glyoxalase I

© 2013 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.Peer reviewedPublisher PD

T-Cell-Replete Haploidentical Stem Cell Transplantation with Post-Transplant Cyclophosphamide for Patients with X-Linked Adrenoleukodystrophy: An Immediate Choice for an Urgent Situation

Inst Crianca HC FMUSP, Oncohematol Unit, Sao Paulo, BrazilHosp Israelita Albert Einstein, Sao Paulo, BrazilUniv Fed Parana, BR-80060000 Curitiba, Parana, BrazilUNIFESP Univ Fed Sao Paulo, Hematol & Bone Marrow Transplantat Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Hematol & Bone Marrow Transplantat Dept, Sao Paulo, BrazilHosp Israelita Albert Einstein, Dept Bone Marrow Transplantat, Sao Paulo, BrazilHosp Clin FMUSP, Sao Paulo, BrazilUNIFESP Univ Fed Sao Paulo, Hematol & Bone Marrow Transplantat Dept, Sao Paulo, BrazilWeb of Scienc

DESENVOLVIMENTO E VALIDAÇÃO DE UM DISPOSITIVO PARA MENSURAÇÃO DA FLEXIBILIDADE POR MEIO DO ACELERÔMETRO EM CRIANÇAS

The objectives of this study were to develop and validate a computerized device to measure joint flexibility; to measure flexibility in children in elementary school aged 8 to 10 years using the Wells bank; to compare the data obtained by the Wells bank and the computerized device developed in the present study and evaluate the usability of the device. 215 volunteers participated in this research, aged between 8 and 10 years, 63.72% (137) were female and 36.28% (78) were male, duly enrolled in an educational institution in the Alto Tietê region. For data collection, the volunteers performed the flexibility test using the Wells bank and the computerized device developed in this research to assess flexibility. The computerized device it was made on a 3D printing technology printer. A usability evaluation was carried out with 15 Physical Education teachers for the product developed through a usability questionnaire based on the System Usability Scale (SUS) form, which contains 10 multiple-choice questions. The data obtained by the objective measures determined were evaluated in terms of frequency, mean and standard deviation. The arithmetic means of the three measurements performed at each stage were calculated. Mann-Whitney and Kruskal-Wallis tests and Spearman's correlation were performed for the investigation. For all analyses, significant differences were considered p<0.05. It is concluded that the computerized device to measure joint flexibility is capable of evaluating the range of motion in degrees, and was qualified as easy to handle by professionals in the field of physical education.Los objetivos de este estudio fueron desarrollar y validar un dispositivo computarizado para medir la flexibilidad articular; medir la flexibilidad en niños en la escuela primaria de 8 a 10 años a través del banco Wells; comparar los datos obtenidos por la base de datos Wells y el dispositivo computarizado desarrollado en el presente estudio y evaluar la usabilidad del dispositivo. Un total de 215 voluntarios participaron en esta investigación, con edades de 8 a 10 años, 63,72% (137) mujeres y 36,28% (78) hombres, debidamente matriculados en una institución educativa de la región del Alto Tietê. Para la recolección de datos, los voluntarios realizaron la prueba de flexibilidad utilizando la base de datos Wells y el dispositivo computarizado desarrollado en esta investigación para evaluar la flexibilidad. El dispositivo fue hecho de una impresora de tecnología de impresión 3D. Se realizó una evaluación de usabilidad con 15 profesores de Educación Física para el producto desarrollado a través del cuestionario de usabilidad basado en el formulario System Usability Scale (SUS) que contiene 10 preguntas de opción múltiple. Los datos obtenidos por las mediciones objetivas determinadas fueron evaluados para la frecuencia, media y desviación estándar. Se calcularon las medias aritméticas de las tres mediciones realizadas en cada etapa. Las pruebas de Mann-Whitney y Kruskal-Wallis y la correlación de Spearman se realizaron para la investigación. Para todos los análisis, se consideraron diferencias significativas p<0,05. Se concluye que el dispositivo computarizado para medir la flexibilidad articular es capaz de evaluar el rango de movimiento en grados, y fue calificado fácil de manejar por profesionales de la educación física.Os objetivos deste estudo foram desenvolver e validar um dispositivo computadorizado para mensurar a flexibilidade articular; mensurar a flexibilidade em crianças no ensino fundamental de 8 a 10 anos por meio do banco de Wells; comparar os dados alcançados pelo banco de Wells e o dispositivo computadorizado desenvolvido no presente estudo e avaliar a usabilidade do dispositivo. Participaram desta pesquisa 215 voluntários, com idade de 8 a 10 anos, sendo 63,72% (137) do sexo feminino e 36,28% (78) do sexo masculino, devidamente matriculados em uma instituição de ensino na região do Alto Tietê. Para coleta de dados, os voluntários realizaram o teste de flexibilidade utilizando o banco de Wells e o dispositivo computadorizado desenvolvido nessa pesquisa para avaliar a flexibilidade.O dispositivo foi confeccionado numa impressora de tecnologia de impressão 3D. Foi realizada uma avaliação da usabilidade com 15 professores de Educação Física para o produto desenvolvido através da interação de usabilidade baseada no formulário System Sustainability Scale (SUS) que contém 10 questões de múltipla-escolha. Os dados obtidos por medidas objetivas determinadas foram avaliadas quanto à frequência, média e desvio padrão. Foram seguidas como médias aritméticas das três medidas realizadas em cada etapa. Para investigar realizou-se os testes de Mann-Whitney e de Kruskal-Wallis e a tradução de Spearman. Para todas as análises foram consideradas diferenças significativas p<0,05.Conclui-se que o dispositivo computadorizado para medir a flexibilidade articular é capaz de avaliar em graus a amplitude de movimento

Delegação de funções ao pessoal auxiliar odontológico pelos cirurgiões-dentistas da cidade de Campina Grande - PB

This study analyses the use of auxiliary personnel by dentists, with the private activity in Campina Grande city. The sample was made by 67 professional, selected between those who are registered in Regional Dental Council - PB. The dates are collect by questionnaire with questions about activities developed by the staff. Observed that 71.7% (48) of dentists employ auxiliary personnel. However, as only 39.6% of those people are registered in the Regional Dental Council. The most delegated activities registered were to schedule appointments (95.8%) and organization of archive and file (89.5%). Results show that exists few use of the auxiliary personnel by dentists and efficiency of dental practice could be greatly improved if they are used appropriatel.Este estudo avaliou a utilização do pessoal auxiliar odontológico pelos cirurgiões-dentistas com atuação em consultórios odontológicos privados no município de Campina Grande-PB. A amostra foi constituída por 67 profissionais selecionados dentre aqueles com registro no Conselho Regional de Odontologia - PB. Para a coleta dos dados, foi elaborado um questionário específico que envolvia as atividades desenvolvidas pelos auxiliares. Dentre os principais resultados, verificou-se que 71,7% (48) dos profissionais utilizam pessoal auxiliar. Contudo, apenas 39,6% (19) dos auxiliares possuem registro no Conselho Regional. Dentre as tarefas realizadas, a marcação de consultas foi a mais delegada (95,8%), seguida da organização dos arquivos e fichários (89,5%). Os resultados permitem concluir que há uma subutilização do pessoal auxiliar pelos dentistas e a eficiência da prática odontológica poderia ser maior se esses auxiliares fossem utilizados adequadamente

Knowledge and interest in medical ethics and bioethics in medical graduation

OBJECTIVES: To evaluate the interest and knowledge about Medical Ethics and Bioethics in medical graduation. METHODS: Transversal and descriptive study. Two different questionnaires were applied with questions about the interest in Medical Ethics and Bioethics and the knowledge about the Brazilian Code of Medical Ethics (CME), one to professors and the other to students. RESULTS: One hundred and one professors and 331 students answered the questionnaires. The Brazilian CME had been read by 86.2% of the teachers and by 100% of the students. The importance given to the discipline Medical Ethics, on a scale from 1 to 5, was similar among teachers and students (4.7 ± 0.7 vs. 4.5 ± 0.8; p = 0.086); however the self-evaluation on knowledge about this subject was higher in the first group (3.4 ± 0.9 vs. 3.2 ± 0.7; p = 0.017). In a block with 9 questions, the right answer was given by 5,0 ± 1.9 of teachers and 5.9 ± 1.5 of students (p < 0,001); the mean of correct answers were related to the reading of the CME. CONCLUSIONS: The present study presents unpublished data about the perception of medical teachers and students about medical ethics and bioethics and can be useful for improving the teaching of these disciplines in our medical schools.OBJETIVOS: Avaliar o interesse e o conhecimento sobre ética médica e bioética na graduação médica. MÉTODOS: Estudo transversal e descritivo. Foram utilizados dois questionários auto-aplicáveis, um para docentes e outro para discentes, com questões sobre o interesse em ética médica e bioética e conhecimento sobre o Código de Ética Médica (CEM). RESULTADOS: Foram avaliados 101 professores e 331 estudantes. O CEM brasileiro foi lido por 86,2% dos professores e 100% dos alunos. A importância dada à disciplina de ética médica, numa escala de 1 a 5, foi semelhante entre professores e estudantes (4,7 ± 0,7 vs. 4,5 ± 0,8; p = 0,086), enquanto o autoconhecimento sobre o tema foi maior no primeiro grupo (3,4 ± 0,9 vs. 3,2 ± 0,7; p = 0,017). De um total de nove questões avaliadas sobre o conhecimento do CEM, a média de acertos foi de 5,0 ± 1,9 questões para os professores e de 5,9 ± 1,5 para os acadêmicos (p < 0,001), sendo os acertos correlacionados positivamente com a leitura do código. CONCLUSÕES: Este estudo fornece um panorama inédito sobre a percepção de professores e estudantes de Medicina sobre a ética médica e bioética, podendo servir para fundamentar a melhora do ensino dessa disciplina em nossas faculdades.Universidade Federal da BahiaEscola Bahiana de Medicina e Saúde PúblicaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Hospital São PauloInstituto do coração da Bahia Hospital Ana NeryUniversidade Federal da Bahia Hospital Universitário Professor Edgar SantosUNIFESP, EPM, Hospital São PauloSciEL

Comparison of the acute effects of traditional versus high velocity resistance training on metabolic, cardiovascular, and psychophysiological responses in elderly hypertensive women

Objectives: The aim of the present study was to compare the acute effects of traditional resistance training (RT) versus high velocity RT (HVRT) on metabolic, cardiovascular, and psychophysiological responses in elderly hypertensive women. Methods: Fifteen elderly women (mean age ± standard deviation, 67.1±6.9 years) classified as having hypertension stage 1 or 2 were randomly allocated to complete traditional RT or HVRT; 1 week later, subjects allocated to RT completed the HVRT session and vice-versa. Heart rate, blood pressure, affective response, perceived effort, and blood samples analyzing lactate, nitrate, nitrite, oxidative damage (thiobarbituric acid reactive substances [TBARS]), and 6-hydroxy-2,5,7,8- tetramethylchroman-2-carboxylic acid equivalent antioxidant capacity (TEAC) collected before and after training sessions were assessed. Nutritional counseling was provided regarding nutrients that could affect cardiovascular and nitrate/nitrite analysis. Results: Systolic blood pressure was not statistically different (p.0.05) between conditions at the beginning and during 30 minutes after sessions. Diastolic blood pressure, rate pressure product, and heart rate were not statistically different (p.0.05) between conditions at the begin- ning and during 45 minutes after sessions. Nitric oxide was significantly higher (p,0.0005) for HVRT compared to RT after 30 minutes of exercise. TBARS and TEAC were significantly higher (p,0.05) for HVRT compared with RT only immediately after exercise. There were no differences for psychophysiological variables between protocols. Conclusion: The acute cardiovascular and metabolic responses, including oxidative stress, are transient and within normal values. Taken together with the positive affective responses, both HVRT and RT with this intensity and volume seem to be safe for elderly hypertensive women under medication

Studies of the mass composition of cosmic rays and proton-proton interaction cross-sections at ultra-high energies with the Pierre Auger Observatory

In this work, we present an estimate of the cosmic-ray mass composition from the distributions of the depth of the shower maximum (Xmax) measured by the fluorescence detector of the Pierre Auger Observatory. We discuss the sensitivity of the mass composition measurements to the uncertainties in the properties of the hadronic interactions, particularly in the predictions of the particle interaction cross-sections. For this purpose, we adjust the fractions of cosmic-ray mass groups to fit the data with Xmax distributions from air shower simulations. We modify the proton-proton cross-sections at ultra-high energies, and the corresponding air shower simulations with rescaled nucleus-air cross-sections are obtained via Glauber theory. We compare the energy-dependent composition of ultra-high-energy cosmic rays obtained for the different extrapolations of the proton-proton cross-sections from low-energy accelerator data

Study of downward Terrestrial Gamma-ray Flashes with the surface detector of the Pierre Auger Observatory

The surface detector (SD) of the Pierre Auger Observatory, consisting of 1660 water-Cherenkov detectors (WCDs), covers 3000 km2 in the Argentinian pampa. Thanks to the high efficiency of WCDs in detecting gamma rays, it represents a unique instrument for studying downward Terrestrial Gamma-ray Flashes (TGFs) over a large area. Peculiar events, likely related to downward TGFs, were detected at the Auger Observatory. Their experimental signature and time evolution are very different from those of a shower produced by an ultrahigh-energy cosmic ray. They happen in coincidence with low thunderclouds and lightning, and their large deposited energy at the ground is compatible with that of a standard downward TGF with the source a few kilometers above the ground. A new trigger algorithm to increase the TGF-like event statistics was installed in the whole array. The study of the performance of the new trigger system during the lightning season is ongoing and will provide a handle to develop improved algorithms to implement in the Auger upgraded electronic boards. The available data sample, even if small, can give important clues about the TGF production models, in particular, the shape of WCD signals. Moreover, the SD allows us to observe more than one point in the TGF beam, providing information on the emission angle