The Influence of Two Different Breeding Systems on Quality and Clotting Properties of Milk from Dairy Buffaloes Reared in Sicily (Italy)

This study focuses on nine buffalo farms (a total of 692 buffaloes), five intensive herds (161 lactating buffalo cows) and four semiextensive herds (111 lactating buffalo cows), which are the most common in Sicily. The average milk and cheese yield, fat, protein and lactose contents were very similar between the two breeding systems. Milk casein was higher (P=0.04) in intensive herds than in semiextensive herds (4.07% vs 3.51%), whereas the urea content was higher in semi-extensive herds (40.68 mg/dL vs 37.50 mg/dL), yet within the normal range for buffalo milk. As regards milk pH and titration acidity (°SH), no difference was observed between the two breeding systems. Milk produced by the intensive herds scored the best clotting ability with shorter curd firming time (k20 3.08 min) and greater curd firmness (a30 32.69 mm). Milk produced by the semi-extensive herds, instead, was characterized by a longer curd firming time (k20 5.26 min) and lower curd firmness (a30 27.33 mm). This milk from buffaloes bred in Sicily (Italy) also showed a good chemical composition and clotting ability in particular when produced by the intensive herds

Godišnje promjene nekih pokazatelja mijene tvari u mliječnih krava na području Sredozemlja.

The seasonal rhythms reflect the ability of the endogenous adaptive mechanism to react in advance to the regular environmental changes associated with the seasons. It seems that these biochemical parameters are often influenced by a change in physiological status. The aim of this study was to assess the trend of annual variations of some biochemical parameters in dairy cows. The experimental subjects were ten dairy cows and the start of the experimental period coincided with the last month of pregnancy. These animals were monitored for one year and blood samples were collected every month. From the obtained sera, total bilirubin, blood urea nitrogen (BUN), creatinine, total cholesterol, non-esterified fatty acids (NEFA), tryglicerides, β-hydroxybutyrate, total proteins, calcium and phosphorus were assessed by means of an automated spectrophotometer. All the results were expressed as mean ± standard deviation (SD). Data were normally distributed (P<0.05, Kolmogorov-Smirnov test). One method of repeated measurement, the Analysis of Variance (ANOVA), was applied to determine the effect of time during the experimental period (12 months) and P values of <0.05 were considered statistically significant. Using cosinor-rhythmometry, three rhythmic parameters were determined: mesor (mean level), amplitude (half the range of oscillation) and acrophase (Φ, time of peak). A significant effect of time (P<0.0001) was shown for all the parameters, except for NEFA. After application of cosinor rhythmometry, only total bilirubin, creatinine, triglycerides and β-hydroxybutyrate showed a seasonal rhythm. In conclusion we can affirm that our results supplement the current information available about the circannual changes in the metabolic activity of these animals and help us to evaluate the adaptation of these animals to environmental changes, together with the metabolic effect of calving and lactation.Ritam promjene godišnjih doba utječe na endogeni adaptacijski mehanizam kako bi on unaprijed reagirao na sezonske promjene u okolišu. Čini se da promjene u fiziološkom stanju često utječu na biokemijske pokazatelje vezane uz taj mehanizam. Svrha je ovog istraživanja procijeniti utjecaj godišnjih promjena na neke biokemijske pokazatelje u mliječnih krava. U pokus je bilo uzeto 10 mliječnih krava, a sam je pokus započeo kad su one bile u posljednjem mjesecu bređosti. Krave su bile promatrane tijekom jedne godine, a krv im je bila uzimana svaki mjesec. Uzeti uzorci seruma bili su automatskim spekrofotometrom pretraženi na ukupni bilirubin, dušik iz ureje u krvi (BUN), kreatinin, ukupni kolesterol, neesterificirane masne kiseline, trigliceride, β-hidroksibutirat, ukupne bjelančevine te kalcij i fosfor. Rezultati su bili izraženi kao srednja vrijednost ± standardna devijacija (SD). Podatci su bili normalno distribuirani (P<0,05, Kolmogorov-Smirnov test). Za ponovljeno mjerenje bila je primijenjena analiza varijance (ANOVA) kako bi se odredio učinak vremena tijekom pokusnog razdoblja (12 mjeseci) te su P vrijednosti <0,05 smatrane statistički značajnima. Uporabom Cosinor ritmometrije određena su četiri ritmička pokazatelja: mesor (srednja vrijednost), amplituda (polovica područja oscilacije) i akrofaza (Φ, vršno vrijeme). Značajan učinak vremena (P<0,0001) dokazan je za sve pokazatelje, osim za neesterificirane masne kiseline. Nakon primjene cosinor ritmometrije samo su ukupni bilirubin, kreatinin, trigliceridi i β-hidroksibutirat pokazivali sezonski ritam. Zaključno se može potvrditi da rezulati predstavljaju doprinos aktualnim informacijama o godišnjim promjenama metaboličke aktivnosti u krava i pomažu vrednovanju njihove prilagodbe okolišnim promjenama kod teljenja i laktacije

Ripening-induced changes in microbial groups of artisanal Sicilian goats' milk cheese.

Changes in the microbial flora of "Caprino dei Nebrodi", a raw goat's milk cheese produced in Sicily, were studied during ripening. From 2 batches of cheese, 4 samples were taken at day 0, 2, 15, and 30 of ripening. Also, samples of curd and milk used in the manufacturing process were analyzed. By the end of the ripening process (day 30), high log10 cfu/g were found for Lactobacilli (7.20), Lattococci (7.10), and Enterococci (7.00), whereas counts of Enterobacteriaceae (3.91), Escherichia coli (3.30), and Staphylococcus (3.89) were found to be lower. The study provides useful information on the microbiological properties of "Caprino dei Nebrodi" cheese, and the results obtained suggest that in order to increase the quality of this artisanal product, it is necessary to improve the sanitary conditions of milking and cheese-making. The study was intended as a preliminary step towards the isolation and identification of bacterial species found in this type of goat's cheese

Effect of Molasses and Dried Orange Pulp as Sheep Dietary Supplementation on Physico-Chemical, Microbiological and Fatty Acid Profile of Comisana Ewe's Milk and Cheese

The use of agro-industrial by-products for ruminant feed represents both an economical and environmental convenient way for reducing waste discharge and waste management costs for food industries. Large amounts of waste from citrus processing industries are available in Sicily, Italy. In the present study, the effect of dried citrus pulp as sheep dietary supplementation was evaluated on physico-chemical, microbiological and fatty acid composition of resulting milk and cheese. Pelleted feed integrated with molasses and blond orange pulp, replacing cane molasses, beet pulp and part of the maize and sunflower in ration, were administrated to ewes as an experimental treatment The experiment involved sixty Comisana breed sheep divided into two groups and two feeding trials (experimental and control). Ewe's milk and cheese samples were collected from January to April and analyzed for physico-chemical, microbiological and fatty acid profile composition. Results suggested that both the experimental milk and cheese were different from the controls. In particular, an increase of experimental milk yield and fat content were registered whilst the cheese samples exhibited a significant decrease of pH values and an increase in fat and protein contents. In addition, an increase of conjugated linoleic acids as well as of the oxidative stability were observed indicating the beneficial effect of dietary supplementation. Furthermore, among the main microbial groups, the experimental and control samples, no differences were detected. However, with the exception of streptococci, which was found higher in experimental cheeses, and staphylococci, which was significantly reduced by experimental feed. Moreover, the application of culture-independent methods highlighted the dominance of Lactobacillus rhamnosus/casei group in the experimental cheese, suggesting a driving role of the dietary supplementation in the cheese microbiota composition. The present study demonstrated that the inclusion of citrus by-products in the diet of small dairy ruminants is a promising feeding, which could positively affect milk composition and cheese manufacture

LIPID AND PROTEIN CONTENT IN RAINBOW TROUT IN RELATION TO GONADAL GROWTH

Svrha ovog istraživanja bila je istražiti različite tipove lipida kao i sadržaj proteina u jajnicima kalifornijske pastrve. Ukupno 80 uzoraka Oncorhynchus mykiss (Walbaum 1792) prikupljeno je iz mrijestilišta Kokernag i Verinag (Jammu i Kashmir, Indija) između 2017. i 2019. godine. Zreli stadij gonada kod ženki imao je najviši gonadosomatski indeks (GSI) i minimalni GSI u potrošenom stadiju. Veći sadržaj lipida u jajniku zabilježen je tijekom zrele faze (36%) u usporedbi s drugim fazama prije parenja. Slično, klase lipida također su pokazale fluktuacije tijekom razvoja jajnika. Klase lipida, tj. glikolipidi, slobodne masne kiseline, kolesterol i trigliceridi, imale su veći sadržaj u zrelom stadiju, dok je sadržaj fosfolipida bio veći u zrelom stadiju. Sadržaj proteina također je bio veći u zrelom jajniku, nego u drugim razvojnim fazama. Studija daje referentne vrijednosti za različite biokemijske parametre kalifornijske pastrve koji bi mogli biti korisni za programe praćenja populacije i za razvoj prehrane i metoda upravljanja u kontroliranim uvjetima.The purpose of this study was to examine various lipid classes as well as the protein content in rainbow trout ovaries. A total of 80 samples of Oncorhynchus mykiss (Walbaum 1792) were collected from Kokernag and Verinag hatcheries (Jammu and Kashmir, India) between 2017 and 2019. The mature stage in female fish had the highest gonadosomatic index (GSI) and minimal GSI in the spent stage. Higher lipid content of the ovary was reported during the mature stage (36%), as compared to other pre-breeding stages. Similarly, lipid classes also showed fluctuation during the development of the ovary. The lipid classes, i.e. glycolipids, free fatty acids, cholesterol and triglycerides, had a higher content in the mature stage, while the phospholipid content was higher in the mature stage. The protein content was also higher in the mature ovary than in the other developmental stages. The study provides reference values for various biochemical parameters in rainbow trout that could be useful for population monitoring programs and for the development of diets and management methods for fish production under controlled conditions

Effect of Parity on Claw Horn Lesions in Holstein Dairy Cows: Clinical and Radiological Study

AbstractThe aim of this study was to estimate the effect of parity on claw lesions prevalence in Holstein dairy cows through clinical and radiological observations. Ninety-seven cows were examined two months after calving to record body condition score, locomotion score, feet diagnostic imaging (X-ray), hoof trimming and clinical evaluation. According to the lactation, the cows were divided into three groups: first parity (Group 1, n=40), second parity (Group 2, n= 29) and third or more parity (Group 3, n=28). Several claw lesions were found. One-way analysis of variance was applied along with Pearson's correlation coefficient. Significant effect of groups was evidenced on radiographic images. In particular, the radiographic images showed a significant increase of osteolysis from Group 1 to Group 3. The application of Pearson's correlation coefficient showed a significantly positive correlation between locomotion score and some claw lesions in all groups. In conclusion, this study provides an overview of claw lesions in dairy cow through clinical and radiological observations

Variation in Structure and Process of Care in Traumatic Brain Injury: Provider Profiles of European Neurotrauma Centers Participating in the CENTER-TBI Study.

INTRODUCTION: The strength of evidence underpinning care and treatment recommendations in traumatic brain injury (TBI) is low. Comparative effectiveness research (CER) has been proposed as a framework to provide evidence for optimal care for TBI patients. The first step in CER is to map the existing variation. The aim of current study is to quantify variation in general structural and process characteristics among centers participating in the Collaborative European NeuroTrauma Effectiveness Research in Traumatic Brain Injury (CENTER-TBI) study. METHODS: We designed a set of 11 provider profiling questionnaires with 321 questions about various aspects of TBI care, chosen based on literature and expert opinion. After pilot testing, questionnaires were disseminated to 71 centers from 20 countries participating in the CENTER-TBI study. Reliability of questionnaires was estimated by calculating a concordance rate among 5% duplicate questions. RESULTS: All 71 centers completed the questionnaires. Median concordance rate among duplicate questions was 0.85. The majority of centers were academic hospitals (n = 65, 92%), designated as a level I trauma center (n = 48, 68%) and situated in an urban location (n = 70, 99%). The availability of facilities for neuro-trauma care varied across centers; e.g. 40 (57%) had a dedicated neuro-intensive care unit (ICU), 36 (51%) had an in-hospital rehabilitation unit and the organization of the ICU was closed in 64% (n = 45) of the centers. In addition, we found wide variation in processes of care, such as the ICU admission policy and intracranial pressure monitoring policy among centers. CONCLUSION: Even among high-volume, specialized neurotrauma centers there is substantial variation in structures and processes of TBI care. This variation provides an opportunity to study effectiveness of specific aspects of TBI care and to identify best practices with CER approaches

Variation in monitoring and treatment policies for intracranial hypertension in traumatic brain injury: A survey in 66 neurotrauma centers participating in the CENTER-TBI study

The distributions of species are not only determined by where they can survive – they must also be able to reproduce. Although immigrant inviability is a well-established concept, the fact that immigrants also need to be able to effectively reproduce in foreign environments has not been fully appreciated in the study of adaptive divergence and speciation. Fertilization and reproduction are sensitive life-history stages that could be detrimentally affected for immigrants in non-native habitats. We propose that “immigrant reproductive dysfunction” is a hitherto overlooked aspect of reproductive isolation caused by natural selection on immigrants. This idea is supported by results from experiments on an externally fertilizing fish (sand goby, Pomatoschistus minutus). Growth and condition of adults were not affected by non-native salinity whereas males spawning as immigrants had lower sperm motility and hatching success than residents. We interpret these results as evidence for local adaptation or acclimation of sperm, and possibly also components of paternal care. The resulting loss in fitness, which we call “immigrant reproductive dysfunction,” has the potential to reduce gene flow between populations with locally adapted reproduction, and it may play a role in species distributions and speciation.</p