Exome resequencing. Arrhythmogenic cardiomyopathy: a case of study

Today massively parallel DNA sequencing platforms are become widely available, reducing the costs and the time of DNA sequencing. Next Generation Sequencers (NGSs) allow to obtain large amount of data and they open new perspectives in fields like genomic and medical research. One of the most promising application in medical research and in diagnostic is the exome sequencing,a specific targeted re-sequencing of the known exons. There are two advantage in sequencing the exome: - The human exome is the 1% of the total genome (about 30Mbp) and it is so possible to obtain high coverage with low costs. - Several variations in exome cause diseases. These two features make the exome sequencing very interesting and increasingly used by scientists. There are several strategies for exome sequencing but, we considered Illumina and SOLiD approaches. In details, we analyzed 6 patients affected by arrhythomogenic cardiomyopathy. Genetic variations in these patients were already characterized with Sanger technologies so we could compare different variant detections algorithm with SOLiD reads and with Illumina reads. Results confirmed the key role of coverage in detecting variants

SIMULAÇÃO DO APROVEITAMENTO DA ENERGIA EÓLICA PARA IRRIGAÇÃO NO DISTRITO DE IRRIGADO BAIXO-ACARAÚ – CE

A geração de energia elétrica proveniente de sistemas eólicos surge como uma alternativa de grande potencial para a região Nordeste do Brasil, podendo promover o desenvolvimento econômico e social da região. Os sistemas de irrigação utilizados nos perímetros irrigados demandam quantidades significativas de energia, o que aumenta consideravelmente o custo de produção final. O trabalho objetivou a simulação da geração de energia elétrica provinda da fonte eólica como alternativa energética para o perímetro irrigado do Baixo-Acaraú, CE. Para tal foi utilizado o software EOLUSOFT – Versão Beta 1.0 NUTEMA-PUCRS desenvolvido na PUC do Rio Grande do Sul, pelo Núcleo Tecnológico de Energia e Meio Ambiente, e no bombeamento de água foi considerada uma bomba d água de 5 cv com capacidade para bombear 10.800 l/h. Como resultado da simulação o sistema eólico apresentou-se possível de atender as necessidades de alimentação elétrica rural no perímetro irrigado com as condições de velocidade de vento analisados para o local, sendo capaz de fornecer o abastecimento de água para a irrigação

Agronomic performance of corn culture under different space arrangements in northeast Brazil

O objetivo com este trabalho foi avaliar o desempenho agronômico do milho em função da população de plantas e espaçamentos entre linhas em um Argissolo Vermelho Amarelo no nordeste brasileiro. O experimento foi realizado em área pertencente à Universidade Federal do Ceará, Fortaleza - CE, com delineamento em blocos casualizados no esquema fatorial 2 x 2, com 20 repetições, sendo os tratamentos constituídos por duas populações (45.000 e 65.000 plantas ha-1) e dois espaçamentos (0,45 e 0,90 m). Avaliou-se a produção por hectare, número de espigas por hectare, plantas por hectare, altura da planta, altura de inserção da 1ª espiga, massa seca total, número de grãos por fileiras, número de fileiras, emergência e diâmetro do colmo. Observou-se diferenças significativas entre a população e o espaçamento para a produção por hectare, espigas por hectare, plantas por hectare e emergência. A população de 65.000 plantas ha-1 no espaçamento de 0,90 m proporcionou incremento na produção por hectare, espiga por hectare e emergência, quando comparadas à população de 45.000 plantas ha-1 e espaçamentos entre linhas de 0,45 m.12529762983The objective of this work was to evaluate the agronomic performance of maize as a function of the plant population and line spacings, in a Red Yellow Argisol in northeast Brazil. The experiment was carried out in an area belonging to the Federal University of Ceará, Fortaleza, Brazil, with a randomized complete block design in the 2 x 2 factorial scheme, with 20 replications, with two populations (45,000 and 65,000 plants ha-1) and two spacings (0.45 and 0.90 m). It was evaluated the yield per hectare, number of spikes per hectare, plants per hectare, height of the plant, height of insertion of the first spike, total dry mass, number of grains per row, number of rows, emergence and stem diameter. Significant differences were observed between population and spacing for yield per hectare, spikes per hectare, plants per hectare, and emergence. The population of 65,000 plants ha-1 in the 0.90 m spacing provided an increase in yield per hectare, spike per hectare and emergence, when compared to the population of 45,000 ha-1 plants and row spacings of 0.45 m

Hyperoxemia and excess oxygen use in early acute respiratory distress syndrome : Insights from the LUNG SAFE study

Publisher Copyright: © 2020 The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.Background: Concerns exist regarding the prevalence and impact of unnecessary oxygen use in patients with acute respiratory distress syndrome (ARDS). We examined this issue in patients with ARDS enrolled in the Large observational study to UNderstand the Global impact of Severe Acute respiratory FailurE (LUNG SAFE) study. Methods: In this secondary analysis of the LUNG SAFE study, we wished to determine the prevalence and the outcomes associated with hyperoxemia on day 1, sustained hyperoxemia, and excessive oxygen use in patients with early ARDS. Patients who fulfilled criteria of ARDS on day 1 and day 2 of acute hypoxemic respiratory failure were categorized based on the presence of hyperoxemia (PaO2 > 100 mmHg) on day 1, sustained (i.e., present on day 1 and day 2) hyperoxemia, or excessive oxygen use (FIO2 ≥ 0.60 during hyperoxemia). Results: Of 2005 patients that met the inclusion criteria, 131 (6.5%) were hypoxemic (PaO2 < 55 mmHg), 607 (30%) had hyperoxemia on day 1, and 250 (12%) had sustained hyperoxemia. Excess FIO2 use occurred in 400 (66%) out of 607 patients with hyperoxemia. Excess FIO2 use decreased from day 1 to day 2 of ARDS, with most hyperoxemic patients on day 2 receiving relatively low FIO2. Multivariate analyses found no independent relationship between day 1 hyperoxemia, sustained hyperoxemia, or excess FIO2 use and adverse clinical outcomes. Mortality was 42% in patients with excess FIO2 use, compared to 39% in a propensity-matched sample of normoxemic (PaO2 55-100 mmHg) patients (P = 0.47). Conclusions: Hyperoxemia and excess oxygen use are both prevalent in early ARDS but are most often non-sustained. No relationship was found between hyperoxemia or excessive oxygen use and patient outcome in this cohort. Trial registration: LUNG-SAFE is registered with ClinicalTrials.gov, NCT02010073publishersversionPeer reviewe

A different Venice

The Northern Lagoon is part of the world-famous Venetian Lagoon in the Italian region of Veneto, but, in contrast to the well-known and exploited Central Lagoon, has managed to preserve its original landscape to a high degree. The future of this tranquil landscape, however, is anything but certain. Its further existence calls for landscape design interventions that consider the balance of three dialectical pairs: land & water, human & non-human and nature & artifice. A related comprehensive design strategy has to focus on the entire lagoon as one system where many environmental, morphological, and social singularities coexist

A different Venice

The Northern Lagoon is part of the world-famous Venetian Lagoon in the Italian region of Veneto, but, in contrast to the well-known and exploited Central Lagoon, has managed to preserve its original landscape to a high degree. The future of this tranquil landscape, however, is anything but certain. Its further existence calls for landscape design interventions that consider the balance of three dialectical pairs: land &amp; water, human &amp; non-human and nature &amp; artifice. A related comprehensive design strategy has to focus on the entire lagoon as one system where many environmental, morphological, an

A different Venice

The Northern Lagoon is part of the world-famous Venetian Lagoon in the Italian region of Veneto, but, in contrast to the well-known and exploited Central Lagoon, has managed to preserve its original landscape to a high degree. The future of this tranquil landscape, however, is anything but certain. Its further existence calls for landscape design interventions that consider the balance of three dialectical pairs: land & water, human & non-human and nature & artifice. A related comprehensive design strategy has to focus on the entire lagoon as one system where many environmental, morphological, and social singularities coexist