Virtualization of everyday life in isolation time: impact of digital technologies on parents and adolescent children

Desde el Proyecto EL IMPACTO DE LAS TECNOLOGÍAS DIGITALES EN EL VÍNCULO ENTRE PADRES E HIJOS ADOLESCENTES, DE LA PLATA Y GRAN LA PLATA, presentamos una línea de investigación reciente y su reformulación de preguntas e hipótesis, con resultados preliminares, resituados en contexto de pandemia. Con el propósito de poner en diálogo, problematizar y compartir el desarrollo de nuestro quehacer como investigadores en una coyuntura tan compleja como novedosa que estamos atravesando, abrimos nuevos interrogantes a la vez que diseñamos estrategias en situación. Compartimos las reformulaciones de instrumento y muestra en un marco de flexibilidad metodológica. Y nos referimos al efecto y modo de virtualización de lo cotidiano en familias con hijos adolescentes en relación a tres ejes: usos y novedades de la tecnología digital en pandemia, en adolescentes y adultos; establecimiento de acuerdos y ordenamientos intergeneracionales; efectos y simbolizaciones posibles de lo que se presenta: espacio-tiempo y cuerpo.This paper aims to present a recent line of research and its reformulation of questions and hypotheses, on the impact that new information and communication technologies (ICT) and digital environments have on the relationship between parents and adolescent children, in families of La Plata and Gran La Plata. (2020-2022), traversed by the context of the COVID-19 pandemic. The use of technology, within the framework of the sanitary measures of isolation and social distancing, slowly acquired a character of imposition in the way of bonding with the other, questioning its use by choice. Faced with the current health crisis and its effects on daily life, it was necessary to make place for new explorations from a first survey. Initial reformulation of aspects and times of the methodological strategy resulting in the flexibility of the qualitative design.Facultad de Psicologí

SIK1/SOS2 networks: decoding sodium signals via calcium-responsive protein kinase pathways

Changes in cellular ion levels can modulate distinct signaling networks aimed at correcting major disruptions in ion balances that might otherwise threaten cell growth and development. Salt-inducible kinase 1 (SIK1) and salt overly sensitive 2 (SOS2) are key protein kinases within such networks in mammalian and plant cells, respectively. In animals, SIK1 expression and activity are regulated in response to the salt content of the diet, and in plants SOS2 activity is controlled by the salinity of the soil. The specific ionic stress (elevated intracellular sodium) is followed by changes in intracellular calcium; the calcium signals are sensed by calcium-binding proteins and lead to activation of SIK1 or SOS2. These kinases target major plasma membrane transporters such as the Na+,K+-ATPase in mammalian cells, and Na+/H+ exchangers in the plasma membrane and membranes of intracellular vacuoles of plant cells. Activation of these networks prevents abnormal increases in intracellular sodium concentration

Involvement of SIK3 in Glucose and Lipid Homeostasis in Mice

Salt-inducible kinase 3 (SIK3), an AMP-activated protein kinase-related kinase, is induced in the murine liver after the consumption of a diet rich in fat, sucrose, and cholesterol. To examine whether SIK3 can modulate glucose and lipid metabolism in the liver, we analyzed phenotypes of SIK3-deficent mice. Sik3−/− mice have a malnourished the phenotype (i.e., lipodystrophy, hypolipidemia, hypoglycemia, and hyper-insulin sensitivity) accompanied by cholestasis and cholelithiasis. The hypoglycemic and hyper-insulin-sensitive phenotypes may be due to reduced energy storage, which is represented by the low expression levels of mRNA for components of the fatty acid synthesis pathways in the liver. The biliary disorders in Sik3−/− mice are associated with the dysregulation of gene expression programs that respond to nutritional stresses and are probably regulated by nuclear receptors. Retinoic acid plays a role in cholesterol and bile acid homeostasis, wheras ALDH1a which produces retinoic acid, is expressed at low levels in Sik3−/− mice. Lipid metabolism disorders in Sik3−/− mice are ameliorated by the treatment with 9-cis-retinoic acid. In conclusion, SIK3 is a novel energy regulator that modulates cholesterol and bile acid metabolism by coupling with retinoid metabolism, and may alter the size of energy storage in mice

Multiple Polymorphisms Affect Expression and Function of the Neuropeptide S Receptor (NPSR1)

Peer reviewe

Cantidad, tipo e intermitencia de los saltos en el voleibol masculino

En este trabajo se registraron la cantidad, el tipo y la intermitencia con la que saltan durante los encuentros jugadores de voleibol menores de 21 años. A partir de los datos obtenidos, se encontró que en cada set jugado el armador salta un promedio de 29 veces, seguido por el central con 21 saltos, el opuesto con 20 y el punta­ receptor con 17. La intermitencia con la que saltan los jugadores de voleibol esta comprendida en los intervalos entre los 1 y los 10 y los 21 y los 30 segundos. El conocer estas variables brinda la posibilidad de diseñar mejores programas de entrenamiento deportivo

Análisis descriptivo del básquetbol: Tiempos de juego, tiempos de pausa y distancias recorridas

El objetivo de este trabajo fue analizar una serie de variables importantes para la Preparación Física en Básquetbol como los tiempos totales de juego (tiempos de duración total de los cuartos, tiempos de juego real y los intervalos de tiempos mas repetidos) los tiempos y tipos de pausas (activas y pasivas) y el tipo y cantidad de metros recorridos por las diferentes posiciones (sprint, defensa intensa, defensa ¿ intensidad, carrera rápida, carrera ¿ intensidad, carrera atrás y caminar); también se registraron los saltos. Entiendo que, a la hora de cuantificar las cargas del entrenamiento es primordial establecer una etapa previa de estudio y análisis de las necesidades a las que se someterá el deportista en la competencia para planificar sobre datos concretos y reales

Tiempos de juego y pausa en el voleibol masculino y femenino

En este trabajo se analizan los tiempos de juego y de pausa en el voleibol masculino y femenino. Los intervalos de juego más significativos tanto para los varones como para las mujeres son los comprendidos entre 1 y los 10 segundos. En el caso de los tiempos de pausa, los intervalos que más se repiten son los comprendidos entre los 11 y los 30 segundos. Conocer los tiempos de juego y de pausa, así como la duración de cada set y del partido, permite diseñar programas de entrenamiento acordes a las necesidades de cada categoría

The Dopamine Paradox in Lung and Kidney Epithelia: Sharing the Same Target but Operating Different Signaling Networks

Stimulation of dopamine receptors in the lung or kidney epithelia has distinct and opposite effects on the function of Na,K-ATPase, which results in increased Na+ absorption across the alveolar epithelium and increased sodium excretion via the kidney epithelium. In the lung, dopamine increases Na,K-ATPase by increasing cell basolateral surface expression of Na+,K+-ATPase molecules, whereas in the kidney epithelia it decreases Na+,K+-ATPase activity by removing active units from the plasma membrane by endocytosis. The opposite effects of dopamine over the same target (the Na+,K+-ATPase) involve the activation of a distinct signaling network that it is target specific, and has a different spatial resolution. Understanding the specific signaling pathways involved in these actions of dopamine and their hierarchical organization may facilitate the drug discovery process that could lead to the design of new therapeutic approaches to clear lung edema in patients with acute lung injury and to decrease fluid overload during congestive heart failure and hypertension

Receptor-mediated inhibition of renal Na +

The mechanisms involved in receptor-mediated inhibition of Na + -K + -ATPase remain poorly understood. In this study, we evaluate whether inhibition of proximal tubule Na + -K + -ATPase activity by dopamine is linked to its removal from the plasma membrane and internalization into defined intracellular compartments. Clathrin-coated vesicles were isolated by sucrose gradient centrifugation and negative lectin selection, and early and late endosomes were separated on a flotation gradient. Inhibition of Na + -K + -ATPase activity by dopamine, in contrast to its inhibition by ouabain, was accompanied by a sequential increase in the abundance of the α-subunit in clathrin-coated vesicles (1 min), early endosomes (2.5 min), and late endosomes (5 min), suggesting its stepwise translocation between these organelles. A similar pattern was found for the β-subunit. The increased incorporation of both subunits in all compartments was blocked by calphostin C. The results demonstrate that the dopamine-induced decrease in Na + -K + -ATPase activity in proximal tubules is associated with internalization of its α- and β-subunits into early and late endosomes via a clathrin-dependent pathway and that this process is protein kinase C dependent. The presence of Na + -K + -ATPase subunits in endosomes suggests that these compartments may constitute normal traffic reservoirs during pump degradation and/or synthesis