Validación de Child and Adolescent Mindfulness Measure en escolares de Lima, Perú

The aim of this study was to translate and study the psychometric properties of the Child and Adolescent Mindfulness Measure - CAMM (Greco, Baer, & Smith, 2011). The CAMM was administered to a sample of 2120 students in Lima Metropolitana, boys and girls aged between 10 and 17 years old. After an exploratory and confirmatory factor analysis a single component structure showed good fit with an explained variance of 74%. The CAMM showed an adequate internal consistency (αord = .78). Moreover, differences between the scores obtained by gender and age were analyzed. The new Spanish version of the CAMM with 8 items showed adequate psychometric properties, so it can be used with children and teenagers from Lima  Metropolitana.El presente estudio tuvo como objetivo adaptar y analizar las propiedades psicométricas de la escala Child and Adolescent Mindfulness Measure - CAMM de (Greco, Baer, & Smith, 2011) en una muestra de 2120 estudiantes de ambos sexos, entre 10 y 17 años, ubicados en colegios públicos y privados de Lima Metropolitana. Se realizaron los análisis factoriales, exploratorio y confirmatorio a la escala adaptada lingüísticamente y se evidenció una estructura unifactorial para 8 ítems de los 10 de la escala original, con una varianza explicada del 74%. Con el análisis de confiabilidad se obtuvo como resultado una adecuada consistencia interna, siendo el alfa ordinal de .78. Adicionalmente, se analizaron diferencias entre los puntajes obtenidos segúnsexo y edad. Se concluyó que la versión con 8 ítems de la escala CAMM adaptada posee evidencias de validez y de confiabilidad para evaluar mindfulness/atención plena en niños y adolescentes limeños

C-ferroptosis is an iron-dependent form of regulated cell death in cyanobacteria

Ferroptosis is an oxidative and iron-dependent form of regulated cell death (RCD) recently described in eukaryotic organisms like animals, plants, and parasites. Here, we report that a similar process takes place in the photosynthetic prokaryote Synechocystis sp. PCC 6803 in response to heat stress. After a heat shock, Synechocystis sp. PCC 6803 cells undergo a cell death pathway that can be suppressed by the canonical ferroptosis inhibitors, CPX, vitamin E, Fer-1, liproxstatin-1, glutathione (GSH), or ascorbic acid (AsA). Moreover, as described for eukaryotic ferroptosis, this pathway is characterized by an early depletion of the antioxidants GSH and AsA, and by lipid peroxidation. These results indicate that all of the hallmarks described for eukaryotic ferroptosis are conserved in photosynthetic prokaryotes and suggest that ferroptosis might be an ancient cell death program.Fil: Aguilera, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Berdun, Federico Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; ArgentinaFil: Bartoli, Carlos Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Steelheart Molina, Maria Charlotte. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Alegre, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Bayir, Hülya. University of Pittsburgh; Estados UnidosFil: Tyurina, Yulia Y.. University of Pittsburgh; Estados UnidosFil: Kagan, Valerian E.. University of Pittsburgh; Estados UnidosFil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Pagnussat, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Martin, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentin

Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803

Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803.Instituto de Fisiología Vegeta

Inhibitory Receptors Are Expressed by Trypanosoma cruzi-Specific Effector T Cells and in Hearts of Subjects with Chronic Chagas Disease

We had formerly demonstrated that subjects chronically infected with Trypanosoma cruzi show impaired T cell responses closely linked with a process of T cell exhaustion. Recently, the expression of several inhibitory receptors has been associated with T cell dysfunction and exhaustion. In this study, we have examined the expression of the cytotoxic T lymphocyte antigen 4 (CTLA-4) and the leukocyte immunoglobulin like receptor 1 (LIR-1) by peripheral T. cruzi antigen-responsive IFN-gamma (IFN-γ)-producing and total T cells from chronically T. cruzi-infected subjects with different clinical forms of the disease. CTAL-4 expression was also evaluated in heart tissue sections from subjects with severe myocarditis. The majority of IFN-γ-producing CD4+ T cells responsive to a parasite lysate preparation were found to express CTLA-4 but considerably lower frequencies express LIR-1, irrespective of the clinical status of the donor. Conversely, few IFN-γ-producing T cells responsive to tetanus and diphtheria toxoids expressed CTLA-4 and LIR-1. Polyclonal stimulation with anti-CD3 antibodies induced higher frequencies of CD4+CTAL-4+ T cells in patients with severe heart disease than in asymptomatic subjects. Ligation of CTLA-4 and LIR-1 with their agonistic antibodies, in vitro, reduces IFN-γ production. Conversely, CTLA-4 blockade did not improved IFN-γ production in response to T. cruzi antigens. Subjects with chronic T. cruzi infection had increased numbers of CD4+LIR-1+ among total peripheral blood mononuclear cells, relative to uninfected individuals and these numbers decreased after treatment with benznidazole. CTLA-4 was also expressed by CD3+ T lymphocytes infiltrating heart tissues from chronically infected subjects with severe myocarditis. These findings support the conclusion that persistent infection with T. cruzi leads to the upregulation of inhibitory receptors which could alter parasite specific T cell responses in the chronic phase of Chagas disease

Validación de Child and Adolescent Mindfulness Measure en escolares de Lima, Perú

The aim of this study was to translate and study the psychometric properties of the Child and Adolescent Mindfulness Measure - CAMM (Greco, Baer, & Smith, 2011). The CAMM was administered to a sample of 2120 students in Lima Metropolitana, boys and girls aged between 10 and 17 years old. After an exploratory and confirmatory factor analysis a single component structure showed good fit with an explained variance of 74%. The CAMM showed an adequate internal consistency (αord = .78). Moreover, differences between the scores obtained by gender and age were analyzed. The new Spanish version of the CAMM with 8 items showed adequate psychometric properties, so it can be used with children and teenagers from Lima  Metropolitana.El presente estudio tuvo como objetivo adaptar y analizar las propiedades psicométricas de la escala Child and Adolescent Mindfulness Measure - CAMM de (Greco, Baer, & Smith, 2011) en una muestra de 2120 estudiantes de ambos sexos, entre 10 y 17 años, ubicados en colegios públicos y privados de Lima Metropolitana. Se realizaron los análisis factoriales, exploratorio y confirmatorio a la escala adaptada lingüísticamente y se evidenció una estructura unifactorial para 8 ítems de los 10 de la escala original, con una varianza explicada del 74%. Con el análisis de confiabilidad se obtuvo como resultado una adecuada consistencia interna, siendo el alfa ordinal de .78. Adicionalmente, se analizaron diferencias entre los puntajes obtenidos segúnsexo y edad. Se concluyó que la versión con 8 ítems de la escala CAMM adaptada posee evidencias de validez y de confiabilidad para evaluar mindfulness/atención plena en niños y adolescentes limeños

The roadmap from allergic rhinitis to asthma

Upper and lower airways display some common characteristic described as a holistic model. Because rhinitis may proceed asthma, it is imperative to understand the mechanisms and risk factors responsible for the progression from rhinitis to asthma.Recent findingsSubclinical lower airway abnormalities have been observed in patients with rhinitis without asthma. Recent evidence indicates that these patients may have an increased risk of developing asthma. Environmental and genetic factors can contribute to enhance this possibility. The treatments of rhinitis have also been shown to be beneficial to the lower airway, and therefore potentially modify the natural evolution of the atopic march.SummaryLower airway disease is a good predictor for the roadmap from rhinitis to asthma. The shared genetic link and environmental factors could be essential for this progression. Albeit treatment of rhinitis has a beneficial effect on lower airway defects, their role to prevent the progression to asthma remains yet to be confirmed.Fil: Saranz, Ricard. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Lozano, Alejandro. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Lozano, Natalia Andrea. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Alegre, Graciela. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Ponzio, Marina Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentin

Relationship between exhaled nitric oxide and biomarkers of atopy in children and adolescents with allergic rhinitis[Relación entre el óxido nítrico exhalado y los biomarcadores de atopia en niños y adolescentes con rinitis alérgica]

Introduction: Measurement of the exhaled nitric oxide fraction (FeNO) has been proposed as an indirect and non-invasive method to detect eosinophilic airway inflammation. Allergic rhinitis (AR) is frequently associated with high levels of FeNO. Allergic sensitization can contribute to the recruitment of eosinophils in the airway and the consequent increase in FeNO. Objective: To correlate FeNO values with inflammatory and atopic sensitization biomarkers in patients with AR. Patients and methods: Observational, analytical, cross-sectional study. Children and adolescents with AR without asthma were included. FeNO, blood eosinophil count, total serum IgE were determined and skin tests with aeroallergens were performed by calculating the scores for PPC1 (number of positive allergens), STS2 (sum of millimeters of positive papules) and the atopy index (ratio between STS2/STS1). Spearman's correlation test was used between FeNO and variables of inflammation and atopy. Results: Twenty-eight patients between 6 and 17 years old were included. There was a significant positive correlation between FeNO and blood eosinophils (r = .38; p = .047) and between FeNO and the atopy index (r = .40; p = .03). No correlation was found between FeNO and total serum IgE (r = .24; p = .21), STS1 (r = .20; p = .32) and STS2 (r = .34; p = .08). Conclusion: In children and adolescents with AR, FeNO was correlated with the atopy index and the blood eosinophil count. These last biomarkers could be used as alternatives for FeNO as biomarkers of lower airway inflammation in patients with AR.Fil: Saranz, Ricardo J. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud. Clínica Universitaria Reina Fabiola. Allergy and Immunology Division; ArgentinaFil: Lozano, Natalia A. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud. Clínica Universitaria Reina Fabiola. Allergy and Immunology Division; ArgentinaFil: Lozano, Alejandro. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud. Clínica Universitaria Reina Fabiola. Allergy and Immunology Division; ArgentinaFil: Alegre, Graciela. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud. Clínica Universitaria Reina Fabiola. Allergy and Immunology Division; ArgentinaFil: Robredo, Paula. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud. Clínica Universitaria Reina Fabiola. Allergy and Immunology Division; ArgentinaFil: Visconti, Pilar. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud. Clínica Universitaria Reina Fabiola. Allergy and Immunology Division; ArgentinaFil: Cruz, Álvaro A. Universidade Federal da Bahia; BrasilFil: Cruz, Álvaro A. Fundação ProAR; Brasi

Análisis comparativo de la fracción exhalada de óxido nítrico en niños y en adolescentes con rinitis alérgica y no alérgica

Antecedentes: Existe inflamación bronquial subclínica en pacientes con rinitis alérgica. Son menos las evidencias de inflamación de la vía aérea inferior en rinitis no alérgica.Objetivo: Investigar inflamación de la vía aérea inferior por la fracción exhalada de óxido nítrico (FeNO) en pacientes con rinitis alérgica y rinitis no alérgica sin asma y su asociación con función pulmonar, gravedad de la rinitis y biomarcadores de atopia.Métodos: Estudio transversal de pacientes entre seis y 18 años, con rinitis alérgica o rinitis no alérgica sin asma. Se realizó espirometría, IgE sérica, recuento de eosinófilos hemáticos y FeNO. Se clasificó la rinitis según guía ARIA.Resultados: Se incluyeron 40 pacientes, 28 con rinitis alérgica y 12 con rinitis no alérgica. Los pacientes con rinitis alérgica tuvieron niveles de FeNO más elevados (mediana 36.5 ppb, rango 5-114) que aquellos con rinitis no alérgica (mediana 7 ppb, rango 5-24) (p = 0.0011). La FeNO elevada se asoció con anormalidad espirométrica (RM = 7.14 IC 95 % = 1.04-49.04, p = 0.049). En la rinitis alérgica, existió correlación entre FeNO y eosinófilos en sangre (r = 0.41, p = 0-33).Conclusiones: Los niños y adolescentes con rinitis alérgica tuvieron FeNO más elevada que los pacientes con rinitis no alérgica, que se correlacionó con eosinofilia hemática y función pulmonar alterada.Fil: Saranz, Ricardo José. Universidad Católica de Córdoba. Clínica Universitaria Reina Fabiola; ArgentinaFil: Lozano, Alejandro. Universidad Católica de Córdoba. Clínica Universitaria Reina Fabiola; ArgentinaFil: Lozano, Natalia Andrea. Universidad Católica de Córdoba. Clínica Universitaria Reina Fabiola; ArgentinaFil: Alegre, Graciela. Universidad Católica de Córdoba. Clínica Universitaria Reina Fabiola; ArgentinaFil: Sasia, Laura Verónica. Universidad Católica de Córdoba. Clínica Universitaria Reina Fabiola; ArgentinaFil: Robredo, Paula. Universidad Católica de Córdoba. Clínica Universitaria Reina Fabiola; ArgentinaFil: Ponzio, Marina. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; ArgentinaFil: Cruz, Álvaro. Universidad Federal de Bahía; Brasi

Heat stress induces ferroptosis in a photosynthetic prokaryote

Ferroptosis is an oxidative iron-dependent form of cell death recently described in eukaryotic organisms like animals, plants and parasites. Here we report that a similar process takes place in the cyanobacterium Synechocystis sp. PCC 6803 in response to heat stress. After a heat shock, Synechocystis cells undergo a cell death pathway that can be suppressed by canonical ferroptosis inhibitors or by external addition of calcium, glutathione or ascorbic acid. Moreover, as described for eukaryotic cells ferroptosis, this pathway is characterized by an early depletion of antioxidants, and by lipid peroxidation. As in general prokaryotes membranes contain poorly oxidizable saturated or monounsaturated lipid molecules, it was thought that they were not susceptible to ferroptosis. Interestingly, cyanobacteria contain thylakoid membranes that are enriched in polyunsaturated-fatty-acid-containing phospholipids, which might explain their sensitivity to ferroptosis. These results indicate that all of the hallmarks described for eukaryotic ferroptosis are conserved in photosynthetic prokaryotes and suggest that ferroptosis might be an ancient cell death program.Instituto de Fisiología Vegeta