The EMBL Nucleotide Sequence Database

The EMBL Nucleotide Sequence Database (http://www.ebi.ac.uk/embl), maintained at the European Bioinformatics Institute (EBI) near Cambridge, UK, is a comprehensive collection of nucleotide sequences and annotation from available public sources. The database is part of an international collaboration with DDBJ (Japan) and GenBank (USA). Data are exchanged daily between the collaborating institutes to achieve swift synchrony. Webin is the preferred tool for individual submissions of nucleotide sequences, including Third Party Annotation (TPA) and alignments. Automated procedures are provided for submissions from large-scale sequencing projects and data from the European Patent Office. New and updated data records are distributed daily and the whole EMBL Nucleotide Sequence Database is released four times a year. Access to the sequence data is provided via ftp and several WWW interfaces. With the web-based Sequence Retrieval System (SRS) it is also possible to link nucleotide data to other specialist molecular biology databases maintained at the EBI. Other tools are available for sequence similarity searching (e.g. FASTA and BLAST). Changes over the past year include the removal of the sequence length limit, the launch of the EMBLCDSs dataset, extension of the Sequence Version Archive functionality and the revision of quality rules for TPA data

EMBL Nucleotide Sequence Database: developments in 2005

The EMBL Nucleotide Sequence Database () at the EMBL European Bioinformatics Institute, UK, offers a comprehensive set of publicly available nucleotide sequence and annotation, freely accessible to all. Maintained in collaboration with partners DDBJ and GenBank, coverage includes whole genome sequencing project data, directly submitted sequence, sequence recorded in support of patent applications and much more. The database continues to offer submission tools, data retrieval facilities and user support. In 2005, the volume of data offered has continued to grow exponentially. In addition to the newly presented data, the database encompasses a range of new data types generated by novel technologies, offers enhanced presentation and searchability of the data and has greater integration with other data resources offered at the EBI and elsewhere. In stride with these developing data types, the database has continued to develop submission and retrieval tools to maximise the information content of submitted data and to offer the simplest possible submission routes for data producers. New developments, the submission process, data retrieval and access to support are presented in this paper, along with links to sources of further information

Impact de la correction chirurgicale à l'âge adulte du canal atrioventriculaire partiel

JUSTIFICATION DU TRAVAIL: le canal atrioventriculaire partiel (CAVP) est une cardiopathie congénitale prise en charge le plus souvent chez l'enfant, certains patients ne seront symptomatiquement qu'à l'âge adulte. Il existe peu de données sur la correction chirurgicale du CAVP à l'âge adulte. Le but de cette étude est d'évaluer l'impact de cette correction à l'âge adulte. METHODES: 9patients non opérés et 22 opérés, de plus de 15 ans et 3 mois ont été inclus. Les données de l'évaluation clinique, écho-cardiogrphique, rythmologique ont été recensés lors de la première et la dernière consultation dont la filière de soins de cardiologie congénitale des Bouches-du-Rhône. Les données ont été récoltées grâce à une fiche standardisée. RESULTATS: L'âge moyen au diagnostic était de 34 +- 16,6 ans. Il existait une insuffisance de la valve auriculoventriculaire gauche (IVAVG) plus sévère (p=0,012), uine dilatation plus marquée des cavités droites, chez les patients opérés par la suite (p=0,01). Lors de la chirurgie la commissure septale a systématiquement été suturée. Aucun décès péri-opératoire n'a été relevé et aucune complication majeure de la chirurgie ne s'est produite. Les résultats à llong terme sont excellents , sans décès, avec aucune ré-intervention pour IVAVG. La correction chirurgicale améliore les symptômes, diminue l'importance de l'IVAVG (p=0,027) et le niveau de pression artérielle pulmonaire (PAP) (p<0,05). Cependant, chez les patients opérés, la survenue ou la persistance d'une ASV, est associée à un âge plus tardif lors de la chirurgie (p=0,028), à un niveau de PAP plus élevées (p=0,049) et à une IVAVG plus importante lors de la dernière consultation (p=0,027). CONCLUSION: La correction chirurgicale systématique du canal atrioventriculaire partiel à l'âge adulte est indiquée et cela avec une morbi-mortalité péri-opératoire faible. L'âge idéal de cette chirurgie reste indéterminée mis les bons résultats de celle-ci, associés à la diminution des ASV avec la précocité de cette chirurgie incite à la proposer de façon élective avant quarante ans, ceci d'autant que les résultats à long terme sont excellents.AIX-MARSEILLE2-BU Méd/Odontol. (130552103) / SudocSudocFranceF

A Fast and Generic Method to Identify Parameters in Complex and Embedded Geophysical Models: The Example of Turbulent Mixing in the Ocean

International audienceGeophysical systems are generally described by nonlinear mathematical models. These models often involve partial differential equations like the Navier-Stokes equations, and embed sub-models describing various processes, such as sub-grid turbulent mixing or biological processes (e.g., growth and interactions of living organisms). The resulting models are costly to develop and to run, bringing together scientists from different disciplines. Moreover, the resulting model predictions remain sensitive to various forms of uncertainty

Turbulent parameters identification in the marine surface layer

International audienc

Identification of eddy viscosity profile in the marine surface layer in transient wind conditions

International audienc

Exercise stress CMR reveals reduced aortic distensibility and impaired right-ventricular adaptation to exercise in patients with repaired tetralogy of Fallot.

BackgroundThe aim of our study was to evaluate the feasibility of exercise cardiac magnetic resonance (CMR) in patients with repaired tetralogy of Fallot (RTOF) and to assess right and left ventricular adaptation and aortic wall response to exercise in comparison with volunteers.Methods11 RTOF and 11 volunteers underwent prospective CMR at rest and during exercise. A supine bicycle ergometer was employed to reach twice the resting heart rate during continuous exercise, blood pressure and heart rate were recorded. Bi-ventricular parameters and aortic stiffness were assessed using accelerated cine sequences and flow-encoding CMR. A t-test was used to compare values between groups. A Mann Whitney test was used to compare values within groups.ResultsIn RTOF both ventricles showed an impaired contractile reserve (RVEF rest 36.2±8.3%, +1.3±3.9% increase after exercise; LVEF rest 53.8±6.1%, +5.7±6.4% increase after exercise) compared to volunteers (RVEF rest 50.5±5.0%, +10.4±7.1% increase after exercise, p = 0.039; LVEF rest 61.9±3.1%, +12.2±4.7% increase after exercise, p = 0.014). RTOF showed a reduced distensibility of the ascending aorta during exercise compared to volunteers (RTOF: 3.4±1.9 10-3.mmHg-1 vs volunteers: 5.1±1.4 10-3.mmHg-1; p = 0.027). Ascending aorta distensibility was correlated to cardiac work in the volunteers but not in RTOF.ConclusionRTOF showed an impaired contractile reserve for both ventricles. The exercise unmasked a reduced distensibility of the ascending aorta in RTOF, which may be an early sign of increased aortic rigidity

Right ventricular outflow tract prestenting with AndraStent XXL before percutaneous pulmonary valve implantation

BACKGROUND: The indications for percutaneous pulmonary valve implantation (PPVI) have been extended to include large dysfunctional right ventricular outflow tracts (RVOTs). Prestenting of the RVOT is commonly performed before PPVI in order to ensure a stable landing zone. The AndraStent XXL (AndraMed GmbH, Reutlingen, Germany), a cobalt-chromium stent with semi-open cell design, has unique mechanical properties in this indication but is no longer available in France. AIMS: To assess the efficiency of AndraStent XXL before PPVI. METHODS: In this retrospective multicentre cohort study, 86 AndraStents XXL were implanted in 77 patients in 6 centres. RESULTS: PPVI was indicated mainly for pulmonary regurgitation (75.3%) in native or patched RVOT (88.3%). The stents were manually mounted on balloon catheters and delivered through sheaths using a conventional femoral approach. PPVI was performed successfully in 97.4% of patients after successful prestenting, generally during the same procedure (77.9%). There were no deaths associated with stent implantation, and four patients experienced five complications, mainly stent embolization, including one requiring surgery. Neither stent fracture nor dysfunction were observed in any patient during a mean follow-up of 19.2±8.7months. Stent analysis showed an excellent maximal stent expansion (97.1%) regardless of balloon size. A 22.3%±3.4 stent shortening with a 30mm balloon was observed. CONCLUSIONS: Implantation of large cobalt-chromium AndraStent XXL stents is efficient for prestenting before PPVI.status: publishe