A Trypanosoma cruzi monoclonal antibody that recognizes a superficial tubulin-like antigen

A monoclonal antibody (MAB 10), obtained from mice infected with Trypanosoma cruzi, was found to recognize a superficial antigen in living or fixed parasites. It reacted more strongly with T. cruzi than with related parasites such as T. brucei and Leishmania. In immunoblots it recognized a single trypanosoma polypeptide and also brain tubulin, both of which had the same electrophoretic mobility. Further analysis suggested that the α-tubulin subunit contained the epitope recognized by MAB 10. These results suggest that a surface tubulin-like protein is present in T. cruzi.Peer Reviewe

Los valores de las ONG de Desarrollo en relación con su labor de incidencia política

El modelo de trabajo de las ONGD ha ido evolucionando hacia formas de intervención cada vez más integradas en el campo político. Desde este punto de vista, la presente investigación ha buscado en las declaraciones de valores que hacen las organizaciones de la Coordinadora de ONGD - España puntos de contacto con el campo político, analizando qué parte de dichos principios pueden estar influidos por él. Este trabajo permite, además, ver en cuáles de dichos valores hay un mayor consenso entre las organizaciones, pasando a identificar el contenido político que albergan desde la diversidad de puntos de vista que adoptan. Este trabajo permite afirmar que existe una carga política en buena parte de dichos valores, pero que eso no supone una división clara entre las ONGD de la muestra

Low Power Wireless Smoke Alarm System in Home Fires

A novel sensing device for fire detection in domestic environments is presented. The fire detector uses a combination of several sensors that not only detect smoke, but discriminate between different types of smoke. This feature avoids false alarms and warns of different situations. Power consumption is optimized both in terms of hardware and software, providing a high degree of autonomy of almost five years. Data gathered from the device are transmitted through a wireless communication to a base station. The low cost and compact design provides wide application prospects

Analysis of Plasminogen Genetic Variants in Multiple Sclerosis Patients

Dessa Sadovnick, A.; Alcina, Antonio; Fedetz, María; Matesanz, F.; Vilariño-Güell, Carles; Dessa Sadovnick, A. et. al.Multiple sclerosis (MS) is a prevalent neurological disease of complex etiology. Here, we describe the characterization of a multi-incident MS family that nominated a rare missense variant (p.G420D) in plasminogen (PLG) as a putative genetic risk factor for MS. Genotyping of PLG p.G420D (rs139071351) in 2160 MS patients, and 886 controls from Canada, identified 10 additional probands, two sporadic patients and one control with the variant. Segregation in families harboring the rs139071351 variant, identified p.G420D in 26 out of 30 family members diagnosed with MS, 14 unaffected parents, and 12 out of 30 family members not diagnosed with disease. Despite considerably reduced penetrance, linkage analysis supports cosegregation of PLG p.G420D and disease. Genotyping of PLG p.G420D in 14446 patients, and 8797 controls from Canada, France, Spain, Germany, Belgium, and Austria failed to identify significant association with disease (P = 0.117), despite an overall higher prevalence in patients (OR = 1.32; 95% CI = 0.93–1.87). To assess whether additional rare variants have an effect on MS risk, we sequenced PLG in 293 probands, and genotyped all rare variants in cases and controls. This analysis identified nine rare missense variants, and although three of them were exclusively observed in MS patients, segregation does not support pathogenicity. PLG is a plausible biological candidate for MS owing to its involvement in immune system response, blood-brain barrier permeability, and myelin degradation. Moreover, components of its activation cascade have been shown to present increased activity or expression in MS patients compared to controls; further studies are needed to clarify whether PLG is involved in MS susceptibility.We also thank Généthon, L’Association Française contre les Myopathies (AFM), la Fondation pour l’Aide à la Recherche sur la Sclérose en Plaques (ARSEP), and the Biological Resources Centre (BRC) of The French Multiple Sclerosis Genetics Group (CRB-REFGENSEP). This research was undertaken thanks to funding from the Canada Research Chair [950-228408] and Canada Excellence Research Chair programs [214444], Canadian Institutes of Health Research [MOP-137051], Vancouver Coastal Health Research Institute, the Milan & Maureen Ilich Foundation [11-32095000], and the Vancouver Foundation [ADV14-1597]. Replication studies received funding from the program “Investissements d’avenir” ANR-10-IAIHU-06. Fondo de Investigación Sanitaria (FIS)-Instituto de Salud Carlos III (ISCIII)-Fondos Europeos de Desarrollo Regional (FEDER), Unión Europea [grant numbers P12/00555, PI13/01527, PI13/01466 and PI13/0879 to F.M., A.A. and G.I.] and Junta de Andalucía -FEDER [grant number CTS2704 to F.M.]. B.D. is a Clinical Investigator of the Research Foundation Flanders (FWO-Vlaanderen). A.G. and B.D. are supported by the Research Fund KU Leuven (OT/11/087 and CREA/14/023) and the Research Foundation Flanders (G073415N). A.L.T. reports personal fees from Biogen Idec, Chugai, Medimmune, Teva Innovation, and EMD Serono, and grants and personal fees from Genzyme Sanofi and Roche.Peer reviewe

Violencia de género contra las mujeres: una complicidad silenciada

Gender-based violence is a current problem that has been visible, for a few years, through the media, but which continues to be relegated to a minor social issue, when compared with other forms of violence, and that In any case, it responds to an attitude of guilt and responsibility of abusers, and even of the victims themselves. However, the data we find, with regional variations, are alarming and permeate all societies. Starting from the idea that there is a relationship between gender violence and inequality, this work analyzes the social architecture from which this violence is constructed, legitimates and perpetuates To carry out this objective we use a methodology that covers the bibliographic analysis, and data from different statistical sources and articles. The result suggests that there is a scenario of underrepresentation of women at a social, economic and institutional level that leads to an undervaluation of women, and that leads to a silent acceptance of violence against women, at all levels, which allows us affirm that the violence is structural, surpasses pure physical or verbal aggression in the family context, and that, consequently, there is an institutional complicity.La violencia de género constituye un problema de actualidad que viene siendo visibilizado, desde hace unos años, a través de los medios de comunicación, pero que continúa relegado a una cuestión social menor, si se compara con otro tipo de formas de violencia, y que en cualquier caso responde a una actitud de culpabilidad y responsabilidad de maltratadores, e incluso de las propias víctimas. Sin embargo, los datos que encontramos, con variaciones regionales, son alarmantes y permean todas las sociedades. Partiendo de la idea de que existe relación entre violencia de género y desigualdad, este trabajo analiza la arquitectura social a partir de la cual se construye, legitima y perpetúa esa violencia. Para llevar a cabo este objetivo utilizamos una metodología que abarca el análisis bibliográfico, y datos procedentes de distintas fuentes estadísticas y artículos. El resultado sugiere que existe un escenario de subrepresentación social, económica e institucional de la mujer, que lleva a una subvaloración de la misma, y que da lugar a una aceptación silenciosa de la violencia contra ella, en todos los niveles, lo que nos permite afirmar que la violencia es estructural, sobrepasa la pura agresión física o verbal en el contexto familiar, y que, en consecuencia, existe una complicidad institucional.&nbsp

Intratubular penetration ability in the canal perimeter using HiFlow bioceramic sealer with warm obturation techniques and single cone

The aim of this paper was to evaluate the intratubular penetration percentage in the perimeter of the canals of the calcium silicate-based sealer HiFlow, using three warm obturation techniques, continuous wave (CW) and vertical condensation (VC) with tw

Intratubular penetration capacity of HiFlow bioceramic sealer used with warm obturation techniques and single cone: A confocal laser scanning microscopic study

Objective The aim of this paper was to evaluate the intratubular penetration area of a bioceramic sealer, using continuous wave (CW), vertical condensation (VC) with two different types of gutta-percha (conventional (NG) and bioceramic-coated (BG)) and single cone (SC) technique with BG gutta-percha, in different root thirds. Methods A total of 150 mature single-root human teeth (including incisors, canines and premolars) were prepared and randomly divided into five groups (n = 30). Teeth were filled using a bioceramic sealer (TotalFill BC Sealer HiFlow ®) and two different types of gutta-percha, with CW and VC techniques, the teeth in the control group were filled with SC technique and BG gutta-percha. The teeth were sectioned and evaluated as one-third portions in each case under a confocal laser microscope. The penetration area measurements were carried out with the Autocad ® programme. Data was analyzed using the one-factor ANOVA test (p < 0.05) and Post Hoc Test (p < 0.05). Results The ANOVA Test showed significant differences in the penetration areas of the five obturation techniques (P < 0.05). The Post Hoc Test exhibited significant differences in multiple comparisons (P < 0.05). There was more dentinal tubule penetration in the coronal third than in the apical third in all techniques. Conclusions The intratubular penetration of the bioceramic sealer was influenced by the obturation techniques tested, but not by the different gutta-percha tested. There was more penetration of sealer in the warm obturation techniques than the SC, regardless of the type of gutta-percha used.Odontologí

Influence of the LILRA3 Deletion on Multiple Sclerosis Risk : Original Data and Meta-Analysis

Altres ajuts: Junta de Andalucía (JA)- Fondos Europeos de Desarrollo Regional (FEDER) (grant number CTS2704 to FM).Multiple sclerosis (MS) is a neurodegenerative, autoimmune disease of the central nervous system. Genome-wide association studies (GWAS) have identified over hundred polymorphisms with modest individual effects in MS susceptibility and they have confirmed the main individual effect of the Major Histocompatibility Complex. Additional risk loci with immunologically relevant genes were found significantly overrepresented. Nonetheless, it is accepted that most of the genetic architecture underlying susceptibility to the disease remains to be defined. Candidate association studies of the leukocyte immunoglobulin-like receptor LILRA3 gene in MS have been repeatedly reported with inconsistent results. In an attempt to shed some light on these controversial findings, a combined analysis was performed including the previously published datasets and three newly genotyped cohorts. Both wild-type and deleted LILRA3 alleles were discriminated in a single-tube PCR amplification and the resulting products were visualized by their different electrophoretic mobilities. Overall, this meta-analysis involved 3200 MS patients and 3069 matched healthy controls and it did not evidence significant association of the LILRA3 deletion [carriers of LILRA3 deletion: p = 0.25, OR (95% CI) = 1.07 (0.95-1.19)], even after stratification by gender and the HLA-DRB1*15 : 01 risk allele