Overexpression of SAMDC1 gene in Arabidopsis thaliana increases expression of defense-related genes as well as resistance to Pseudomonas syringae and Hyaloperonospora arabidopsidis

It has been previously described that elevation of endogenous spermine levels in Arabidopsis could be achieved by transgenic overexpression of S-Adenosylmethionine decarboxylase (SAMDC) or Spermine synthase (SPMS). In both cases, spermine accumulation had an impact on the plant transcriptome, with up-regulation of a set of genes enriched in functional categories involved in defense-related processes against both biotic and abiotic stresses. In this work, the response of SAMDC1-overexpressing plants against bacterial and oomycete pathogens has been tested. The expression of several pathogen defense-related genes was induced in these plants as well as in wild type plants exposed to an exogenous supply of spermine. SAMDC1-overexpressing plants showed an increased tolerance to infection by Pseudomonas syringae and by Hyaloperonospora arabidopsidis. Both results add more evidence to the hypothesis that spermine plays a key role in plant resistance to biotic stress

GenIsisWeb : l'eina per a publicar a Internet les bases de dades CDS/ISIS

Lâobjectiu de la ressenya és presentar GenIsisWeb, un programa que assisteix en la publicació de bases de dades a Internet. Després dâuna breu descripció tècnica i partint dâuna base de dades prèviament creada amb CDS/ISIS, es mostra el procés complet per a la seva publicació al Web. | The objective of this review is to introduce GenIsisWeb, a program that assists in publishing databases on the Internet. Following a brief technical description, the entire procedure for the web-based publishing a database (based on one previously created using CDS/ISIS) is shown

GenIsisWeb : la herramienta para publicar en Internet las bases de datos CDS/ISIS

El objetivo de la reseña es presentar GenIsisWeb, un programa que asiste en la publicación de bases de datos en Internet. Tras una breve descripción técnica y partiendo de una base de datos previamente creada con CDS/ISIS, se muestra el proceso completo para su publicación en el Web. | The objective of this review is to introduce GenIsisWeb, a program that assists in publishing databases on the Internet. Following a brief technical description, the entire procedure for the web-based publishing a database (based on one previously created using CDS/ISIS) is shown

Reproducibility of the International Endometrial Analysis Group color score for assigning the amount of flow within the endometrium using stored 3-dimensional volumes

Objectives - To estimate intraobserver and interobserver reproducibility for assigning an International Endometrial Tumor Analysis (IETA) group color score for endometrial vascularization on color Doppler imaging. Methods - Sixty-eight endometrial 3-dimensional volumes from endometrial color Doppler assessments of women with different endometrial disorders were evaluated by 8 different examiners (4 skilled examiners and 4 obstetric and gynecologic trainees). One skilled examiner who did not participate in the assessments selected the 68 volumes from a database to select a balanced number of each IETA score. Each examiner evaluated the 68 endometrial volumes to assign the IETA color score (1, absence of vascularization; 2, low vascularization; 3, moderate vascularization; or 4, abundant vascularization) using tomographic ultrasound imaging. The analysis was repeated 4 weeks later, and interobserver and intraobserver reproducibility was analyzed by calculating the weighted j index. The second of the measurements made by each observer was used to estimate interobserver reproducibility. Results - The intraobserver reproducibility was very good for all examiners, with a weighted j index ranging from 0.84 to 0.91. The interobserver reproducibility was good or very good for all estimated comparisons, with a weighted j index ranging from 0.77 to 0.96, regardless of experience level. Conclusions - The reproducibility of assigning the IETA color score for assessing endometrial vascularization using 3-dimensional volumes is good or very good regardless of the experience of the examiner