Análise quantitativa de mastócitos na cicatrização de feridas tratadas com membranas de colágeno contendo própolis vermelha

Mast cells are connective tissue cells responsible for initiating the inflammatory reaction and chronicity of the process, and play an important role in the dynamics of the repair scar. The use of natural or synthetic biological membranes in the repair of extensive dermal wounds, in turn, has been widely discussed in the literature, particularly those based on collagen, due to biocompatibility and interactivity of these materials. Propolis is a natural product that presents anti-inflammatory properties, so that it could be useful to the repair scar. The red variety of this product, however, is still less studied, although there are some reports of a probable healing action. Thus, the objective of this study was to analyze the effect of the combination of collagen membranes to red propolis on the mast cells population during repair scar by second intention in rats. For this purpose, membranes were prepared from collagen extracted from bovine tendon (10-15mm thick) containing hydroalcoholic extract of red propolis to 0.1%. Subsequently, 1cm2 standard-sized wounds were performed in th back of 30 Wistar rats, which were divided into six groups (n=5): G1- untreated animals sacrificed at 7 days; G2 – animals treated with collagen membrane sacrificed at 7 days; G3 – animals treated with collagen membrane containing red propolis sacrificed at 7 days; G4 - untreated animals sacrificed at 14 days; G5 – animals treated with collagen membrane sacrificed at 14 days; and G6 – animals treated with collagen membrane containing red propolis sacrificed at 14 days. The removed specimens were fixed, histologically processed and embedded in paraffin and histological sections were stained with toluidine blue. On the seventh day, the average population of mast cells in total and marginal G1 was significantly lower than in G2 and G3 (P<0.05), but there was no difference between these last two groups. At the fourteenth day, it was not verified any statistically significant difference in the mean of mast cells among the three groups. These data suggest that, in mice, the use of bioactive collagen membranes, containing or not 0.1% hydroalcoholic extracts of red propolis, can reduce the degranulation of mast cells after seven days of healing of wounds, but has no effect on this population of immunocompetent cell in the final stages (14 days) repair.Los mastocitos son células del tejido conectivo responsables por el inicio de la reacción inflamatoria y la cronicidad del proceso y juegan un papel importante en la dinámica de la reparación cicatricial. El uso de membranas biológicas naturales o sintéticas en la reparación de heridas dérmicas extensas, a su vez, ha sido ampliamente discutido en la literatura, especialmente los basados en colágeno, debido a la biocompatibilidad y la interacción del material. El própolis es un producto natural que tiene actividad antiinflamatoria, que puede contribuir a la reparación cicatricial. La variedad roja de este producto, sin embargo, ha sido poco estudiada, y hay algunos informes de una probable acción cicatrizante. De esta manera, el objetivo de este estudio fue analizar el efecto de la combinación de membranas de colágeno y própolis rojo en la población de mastócitos durante la reparación  cicatricial  por segunda intención en ratos. Para este propósito, las membranas se prepararon a partir de colágeno I extraído de tendón de bovino (10-15 m m de espesor) que contiene extracto hidroalcohólico de própolis a 0,1%. Posteriormente, hicieron heridas de 1cm2 sobre el dorso de 30 ratones Wistar , que se dividieron en seis grupos (n = 5): G1 - animales no tratados sacrificados a los 7 días, G2 - animales tratados con membrana de colágeno sacrificados a los 7 días; G3 -  animales tratados con membrana de colágeno contieniendo própolis roja  sacrificados después de 7 días; G4 - los animales no tratados sacrificados a los 14 días; G5 - animales tratados con membrana de colágeno sacrificado a los 14 días; G6 - animales tratados con membrana de colágeno  contieniendo própolis roja  sacrificados en 14 días. Las muestras extraídas se fijaron, se procesaron y se agruparon en parafina, se hicieron cortes histológicos y se tiñeron con azul de toluidina. En el séptimo día, el promedio de la población de mastocitos en G1 total y marginal fue significativamente menor que en el G2 y G3 (p <0,05), pero no hubo diferencias entre los dos últimos. En el día catorce, no hubo diferencias estadísticamente significativas entre el G4, G5 y G6 ni en el promedio total de mastócitos, ni en las dos regiones estudiadas por separado. Se sugiere que, en ratones, la utilización de membranas de colágeno bioactivos, independiente o no de contener própolis rojo en forma de extracto hidroalcohólico 0,1%, puede reducir la degranulación de los mastocitos después de siete días de cicatrización de heridas abiertas, pero no tiene efecto sobre la población de esta célula inmunocompetente en etapas finales (14 días) de reparación.Mastócitos são células do tecido conjuntivo responsáveis pelo início da reação inflamatória e cronificação do processo, e desempenham um papel importante na dinâmica do reparo cicatricial. O uso de membranas biológicas naturais ou sintéticas no reparo de feridas dérmicas extensas, por sua vez, tem sido bastante discutido na literatura, especialmente aquelas à base de colágeno, devido à biocompatibilidade e interatividade desse material. A própolis é um produto natural que apresenta atividade anti-inflamatória, podendo contribuir para a reparação cicatricial. A variedade vermelha desse produto, contudo, ainda é pouco estudada, existindo alguns relatos de uma provável ação cicatrizante. Assim, o objetivo deste trabalho foi analisar o efeito da associação de membranas de colágeno à própolis vermelha sobre a população de mastócitos durante o reparo cicatricial por segunda intenção em ratos. Para tanto, foram preparadas membranas de colágeno I extraído de tendão bovino (10-15mm de espessura) contendo extrato hidroalcóolico de própolis vermelha a 0,1%. Posteriormente, foram realizadas feridas de 1cm2 no dorso de 30 ratos Wistar, que foram distribuídos em seis grupos (n=5): G1 – animais sem tratamento sacrificados em 7 dias; G2 – animais tratados com membrana de colágeno sacrificados em 7 dias; G3 – animais tratados com membrana de colágeno contendo própolis vermelha sacrificados em 7 dias; G4 – animais sem tratamento sacrificados em 14 dias; G5 – animais tratados com membrana de colágeno sacrificados em 14 dias; G6 – animais tratados com membrana de colágeno contendo própolis vermelha sacrificados em 14 dias. Os espécimes removidos foram fixados, processados histologicamente e emblocados em parafina e secções histológicas foram coradas em Azul de toluidina. Ao sétimo dia, a população média de mastócitos total e marginal em G1 mostrou-se significativamente menor que em G2 e G3 (p<0,05), mas não houve diferença entre os dois últimos. Ao décimo quarto dia não foi observada diferença estatisticamente significativa entre o G4, G5 e G6 nem na média total de mastócitos, nem nas duas regiões estudadas separadamente. Sugere-se que, em ratos, o uso de membranas bioativas de colágeno, independentemente de conter ou não própolis vermelha sob a forma de extrato hidroalcoólico a 0,1%, pode reduzir a desgranulação de mastócitos aos sete dias de cicatrização de feridas abertas, mas não influencia a população desta célula imunocompetente nas fases finais (14 dias) do reparo

Tumores de células granulares na língua: relato de 2 casos

ResumoO tumor de células granulares é uma neoplasia benigna incomum com forte predileção pela cavidade oral, com um maior número de casos afetando a região da língua. Apresenta-se geralmente como um nódulo solitário, assintomático, de base séssil, com crescimento lento (meses ou anos) e coloração variável. Apesar da sua etiologia incerta, há estudos que apontam para uma origem neural para a lesão. O presente artigo tem como objetivo apresentar 2 casos clínicos cuja queixa principal era um nódulo submucoso no dorso da língua, com evolução lenta e superfície epitelial hígida. Em ambos os casos, o diagnóstico definitivo foi realizado após a análise histológica e imuno-histoquímica e o tratamento realizado através de excisão cirúrgica conservadora. Também são discutidos os aspetos clínicos, histológicos e imuno-histoquímicos do tumor de células granulares.AbstractThe granular cell tumor is an uncommon benign neoplasm with a strong predilection for the oral cavity, with a greater number of cases affecting the tongue. It typically presents as a solitary nodule, asymptomatic, sessile base, with slow growth (months or years) and variable color. Despite its uncertain etiology, studies suggest a neural origin for the lesion. The aim of this article is to present two clinical cases whose main complaint was a submucosal nodules in the dorsum of the tongue, with slow evolution and healthy surface. In both cases, definitive diagnosis was made after histological and immunohistochemical analysis and the treatment was made with conservative surgical excision. It is also discussed clinical, histological and immunohistochemical features of granular cell tumor

NÍVEIS DE ESTRESSE EM TAXISTAS

Este estudo teve como objetivo verificar os níveis de estresse em motoristas de táxi em Aracaju/SE, utilizando o Inventário de Sintomas de Estresse de Lipp (ISEL). Foram entrevistados 72 motoristas de táxi os quais 48,6% apresentaram estresse e 51,4% não apresentaram estresse. A idade média bem como o tempo de serviço na presença ou não do quadro de estresse foi semelhante (p>0,05). Sintomas físicos associados ao estresse são os problemas de memória e a tensão muscular. O ato de pensar constantemente em um só assunto mostrou associação significativa com os sinais de estresse (

Diagnosis, management, and follow-up of extensive dermoid cyst of the submental region

Dermoid cyst (DC) is a cystic lesion of developmental origin and uncertain etiology that rarely affects the floor of the mouth. We report a case of a large lesion found in the submental and submandibular region in a 25-year-old male patient. Computed tomography revealed extensive hypodense lesion in the submental and submandibular space without peripheral enhancement. The microscopical analysis showed a cystic cavity lined by orthokeratinized stratified squamous epithelium. The cystic capsule was composed of dense fibrous connective tissue containing cutaneous attachments, such as sebaceous and sweat glands. The diagnosis of DC was made. The differential diagnosis of expansive sublingual lesions can be clinically challenging due to the similarity with several lesions frequently observed in this region. Herein, we describe a case of extensive DC arising in the floor of the mouth, presenting clinical, imaging, and microscopical features

Plasma cell cheilitis: the diagnosis of a disorder mimicking lip cancer

Plasma cell cheilitis (PCC) is an inflammatory disorder of unknown etiology that affects the lip. It is characterized histologically by a dense infiltrate of plasma cells with a variety of clinical features. The response to different therapeutic modalities is controversial, especially regarding the effectiveness of corticosteroids. We present a case of a 56-year-old Caucasian man with a painful ulcerated and crusted area in the lower lip, resembling a squamous cell carcinoma or actinic cheilitis. Topical corticosteroid was used for one week, which resulted in partial regression and motivated a biopsy. The histological examination provided the diagnosis of PCC. The patient has been disease-free for six months. We also provide a discussion on the criteria of differential diagnosis and management of this rare condition. See ERRATUM&nbsp

Trait anxiety affects the orofacial nociceptive response in rats

The aims of the present study were to assess the influence of: a) trait anxiety on orofacial pain; and b) orofacial pain on state anxiety. Forty-four rats were initially exposed to the free-exploratory paradigm for the evaluation of their anxiety profiles. In accordance to the parameter "Percentage of time in the novel side", the animals were considered as presenting high or low levels of trait anxiety when presenting values below the 1st quartile, or above the 3rd quartile, respectively. A week later, formalin-1.5% was injected into the upper lip of each animal. The behavioural nociceptive response, characterized by increased orofacial rubbing (OR), was quantified for 30 minutes, as follows: Total time OR (0-30 minutes: total pain), 1st phase OR (0-6 minutes: neurogenic pain), and 2nd phase OR (12-30 minutes: inflammatory pain). Immediately after this test, but still under the effect of formalin, the rats were submitted to the Elevated Plus-maze test (EPM). The results showed that the high trait anxiety individuals presented higher frequency of OR than the low trait anxiety ones, except during the neurogenic pain period. However, no correlation was found between OR frequency and levels of state anxiety presented on the EPM. In conclusion, the animals presenting higher anxiety profiles were the most susceptible to orofacial pain, nevertheless, orofacial pain did not influence state anxiety

Generalized hereditary gingival fibromatosis in a child: clinical, histopathological and therapeutic aspects

Hereditary gingival fibromatosis (HGF) is a rare genetic condition characterized by slow and progressive gingival enlargement. The gingival overgrowth often delays teeth eruption and may cause serious functional and aesthetic problems. We reported a case of a 10-year-old female child presenting a generalized gingival enlargement covering almost all the maxillary and mandibular teeth and resulted in problems for swallowing, speaking, and poor aesthetics. An incisional biopsy was performed and revealed a hypocellular and hypovascular dense collagenous tissue covered by squamous epithelium exhibiting acanthosis and elongated rete ridges. The diagnosis was HGF. The treatment instituted was an association of gingivectomy with a rigorous program of oral hygiene and follow-up. Herein, we describe a rare non-syndromic case of generalized HGF, including clinical and microscopical features, as well as highlighting the importance of correct diagnosis of this genetic condition