Determination of spatial and temporal water relations in single leaves and canopies based on thermographic measurements

Land plants are exposed to an exceptional challenge, because they need to take up CO2 from a dry atmosphere, which induces water loss while taking up CO2. As the leaf water content (LWC) is a result of an equilibrium between water uptake and water loss through transpiration, it is an important parameter in the overall plant-water relation. Water loss, and thus LWC, is mainly affected by transpiration. Water vapor released through the stomata is restricted by the leaf boundary layer, a thin air layer surrounding the leaf and acting as a heat barrier. Convection that is the heat transfer from the leaf surface into the ambient air via air movement further influences the boundary layer. The relationships between LWC, transpiration, convection, and the leaf boundary layer, are summarized in the leaf energy balance model that relates all these parameters to the leaf temperature (TL). Therefore, thermography, non-invasive and spatial TL measurements is a convenient method to derive information about the plant-water relations. A commonly used parameter is the crop water stress index (CWSI), which empirically relates passively measured TL to rates of transpiration. This work introduces the active thermography where TL is manipulated and transiently increased by exposure to short heat pulses. Resulting cooling curves are measured and quantified with the time constant (t). In theory, t is the product of the leaf heat capacity per unit area, which is proportional to LWC, and the inverse of the leaf heat transfer coefficient (hleaf). hleaf describes the boundary layer conductance to heat and is the sum of heat transfer coefficients for transpiration, convection, and thermal radiation. Using spring barley (Hordeum vulgare) and common bean (Physeolus vulgaris), it was extensively tested whether and under which conditions t can be used to derive LWC. Finally, the active thermography was transferred from the laboratory into the green-house were the applicability to detect mild drought stress on the canopy scale was tested. Under well-defined ambient conditions, t of dark-adapted leaves was proportional to LWC for the both plants species and under varying wind-speed. These relationships were used to map t of whole leaves, providing distribution maps of LWC. A comparison of active thermography measurements with active temperature measurements within a leaf gas exchange cuvette revealed an environment-depending relationship between t and hleaf. While t in the well-ventilated gas exchange cuvette is mainly driven by changes in LWC, t of leaves exposed to wind-free conditions was related to both, LWC and hleaf. Forward modelling of hleaf, showed a strong impact of hleaf on t, which significanTLy differed with wind and illumination. Thus, the relative contribution of the single heat transfer coefficients for the single components (transpiration, convection, and thermal radiation) greatly depended on the environmental conditions. While the heat transfer coefficient for thermal radiation was nearly negligible, the heat transfer for convection and transpiration showed a much stronger impact on hleaf, respectively. Irrespectively of wind-speed and illumination, the heat transfer coefficient for convection contributed most to the overall hleaf. In the dark, the heat transfer coefficient for transpiration had no impact on hleaf. Upon illumination the impact was significant, but became less important with increasing wind-speed. Finally, the active thermography approach was transferred from the laboratory into the green-house, where the applicability for mild drought stress detection on the canopy scale was tested. Four different barley varieties were exposed to a deficit irrigation (DI) treatment. Diurnal and seasonal dynamics of t were observed and compared to the commonly used CWSI. While the CWSI mainly responded to changes in hleaf, t responded to changes in LWC and hleaf. Consideration of both parameters in parallel revealed an overall insight in the plant-water relations, which could be related to canopy water loss rates and biomass production. Therefore, combining t and the CWSI in one single index would be advantageous. A first attempt to develop such an index is made, and the empirical dehydration index is suggested as basis for future development. This work demonstrates, that the active thermography is a valuable tool for a wide range of applications in plant science. The measurement of t can be used to detect dynamics in the plant-water relations, when regarding the environmental conditions properly. Additionally, t may be used in modelling studies, e.g. to model leaf and canopy heat transfer processes or to model water-relations of plants with respect to hydrological cycles of whole ecosystems

Learning and Recognizing Archeological Features from LiDAR Data

We present a remote sensing pipeline that processes LiDAR (Light Detection And Ranging) data through machine & deep learning for the application of archeological feature detection on big geo-spatial data platforms such as e.g. IBM PAIRS Geoscope. Today, archeologists get overwhelmed by the task of visually surveying huge amounts of (raw) LiDAR data in order to identify areas of interest for inspection on the ground. We showcase a software system pipeline that results in significant savings in terms of expert productivity while missing only a small fraction of the artifacts. Our work employs artificial neural networks in conjunction with an efficient spatial segmentation procedure based on domain knowledge. Data processing is constraint by a limited amount of training labels and noisy LiDAR signals due to vegetation cover and decay of ancient structures. We aim at identifying geo-spatial areas with archeological artifacts in a supervised fashion allowing the domain expert to flexibly tune parameters based on her needs

Establishment of a patient-derived orthotopic osteosarcoma mouse model

Background: Osteosarcoma (OS) is the most common pediatric primary malignant bone tumor. As the prognosis for patients following standard treatment did not improve for almost three decades, functional preclinical models that closely reflect important clinical cancer characteristics are urgently needed to develop and evaluate new treatment strategies. The objective of this study was to establish an orthotopic xenotransplanted mouse model using patient-derived tumor tissue. Methods: Fresh tumor tissue from an adolescent female patient with osteosarcoma after relapse was surgically xenografted into the right tibia of 6 immunodeficient BALB/c Nu/Nu mice as well as cultured into medium. Tumor growth was serially assessed by palpation and with magnetic resonance imaging (MRI). In parallel, a primary cell line of the same tumor was established. Histology and high-resolution array-based comparative genomic hybridization (aCGH) were used to investigate both phenotypic and genotypic characteristics of different passages of human xenografts and the cell line compared to the tissue of origin. Results: A primary OS cell line and a primary patient-derived orthotopic xenotranplanted mouse model were established. MRI analyses and histopathology demonstrated an identical architecture in the primary tumor and in the xenografts. Array-CGH analyses of the cell line and all xenografts showed highly comparable patterns of genomic progression. So far, three further primary patient-derived orthotopic xenotranplanted mouse models could be established. Conclusion: We report the first orthotopic OS mouse model generated by transplantation of tumor fragments directly harvested from the patient. This model represents the morphologic and genomic identity of the primary tumor and provides a preclinical platform to evaluate new treatment strategies in OS

Mutations in POLE and survival of colorectal cancer patients – link to disease stage and treatment

Recent molecular profiling studies reported a new class of ultramutated colorectal cancers (CRCs), which are caused by exonuclease domain mutations (EDMs) in DNA polymerase ϵ (POLE). Data on the clinical implications of these findings as to whether these mutations define a unique CRC entity with distinct clinical outcome are lacking. We performed Sanger sequencing of the POLE exonuclease domain in 431 well-characterized patients with microsatellite stable (MSS) CRCs of a population-based patient cohort. Mutation data were analyzed for associations with major epidemiological, clinical, genetic, and pathological parameters including overall survival (OS) and disease-specific survival (DSS). In 373 of 431 MSS CRC, all exons of the exonuclease domain were analyzable. Fifty-four mutations were identified in 46 of these samples (12.3%). Besides already reported EDMs, we detected many new mutations in exons 13 and 14 (corresponding to amino acids 410–491) as well as in exon 9 and exon 11 (corresponding to aa 268–303 and aa 341–369). However, we did not see any significant associations of EDMs with clinicopathological parameters, including sex, age, tumor location and tumor stage, CIMP, KRAS, and BRAF mutations. While with a median follow-up time of 5.0 years, survival analysis of the whole cohort revealed nonsignificantly different adjusted hazard ratios (HRs) of 1.35 (95% CI: 0.82–2.25) and 1.44 (0.81–2.58) for OS and DSS indicating slightly impaired survival of patients with EDMs, subgroup analysis for patients with stage III/IV disease receiving chemotherapy revealed a statistically significantly increased adjusted HR (1.87; 95%CI: 1.02–3.44). In conclusion, POLE EDMs do not appear to define an entirely new clinically distinct disease entity in CRC but may have prognostic or predictive implications in CRC subgroups, whose significance remains to be investigated in future studies

Human Borna disease virus 1 (BoDV-1) encephalitis cases in the north and east of Germany

In 2021, three encephalitis cases due to the Borna disease virus 1 (BoDV-1) were diagnosed in the north and east of Germany. The patients were from the states of Thuringia, Saxony-Anhalt, and Lower Saxony. All were residents of known endemic areas for animal Borna disease but without prior diagnosed human cases. Except for one recently detected case in the state of Brandenburg, all >30 notified cases had occurred in, or were linked to, the southern state of Bavaria. Of the three detected cases described here, two infections were acute, while one infection was diagnosed retrospectively from archived brain autopsy tissue samples. One of the acute cases survived, but is permanently disabled. The cases were diagnosed by various techniques (serology, molecular assays, and immunohistology) following a validated testing scheme and adhering to a proposed case definition. Two cases were classified as confirmed BoDV-1 encephalitis, while one case was a probable infection with positive serology and typical brain magnetic resonance imaging, but without molecular confirmation. Of the three cases, one full virus genome sequence could be recovered. Our report highlights the need for awareness of a BoDV-1 etiology in cryptic encephalitis cases in all areas with known animal Borna disease endemicity in Europe, including virus-endemic regions in Austria, Liechtenstein, and Switzerland. BoDV-1 should be actively tested for in acute encephalitis cases with residence or rural exposure history in known Borna disease-endemic areas.Peer Reviewe

Distinct Mutational Profile of Lynch Syndrome Colorectal Cancers Diagnosed under Regular Colonoscopy Surveillance

Regular colonoscopy even with short intervals does not prevent all colorectal cancers (CRC) in Lynch syndrome (LS). In the present study, we asked whether cancers detected under regular colonoscopy surveillance (incident cancers) are phenotypically different from cancers detected at first colonoscopy (prevalent cancers). We analyzed clinical, histological, immunological and mutational characteristics, including panel sequencing and high-throughput coding microsatellite (cMS) analysis, in 28 incident and 67 prevalent LS CRCs (n total = 95). Incident cancers presented with lower UICC and T stage compared to prevalent cancers (p < 0.0005). The majority of incident cancers (21/28) were detected after previous colonoscopy without any pathological findings. On the molecular level, incident cancers presented with a significantly lower KRAS codon 12/13 (1/23, 4.3% vs. 11/21, 52%; p = 0.0005) and pathogenic TP53 mutation frequency (0/17, 0% vs. 7/21, 33.3%; p = 0.0108,) compared to prevalent cancers; 10/17 (58.8%) incident cancers harbored one or more truncating APC mutations, all showing mutational signatures of mismatch repair (MMR) deficiency. The proportion of MMR deficiency-related mutational events was significantly higher in incident compared to prevalent CRC (p = 0.018). In conclusion, our study identifies a set of features indicative of biological differences between incident and prevalent cancers in LS, which should further be monitored in prospective LS screening studies to guide towards optimized prevention protocols.Peer reviewe

HIV pre-exposure prophylaxis was associated with no impact on sexually transmitted infection prevalence in a high-prevalence population of predominantly men who have sex with men, Germany, 2018 to 2019

Introduction: Despite increased use of pre-exposure prophylaxis (PrEP) in Germany, HIV infection rates are not declining and little is known about how this prevention method affects the prevalence of sexually transmitted infections (STI) among men who have sex with men (MSM). Aim: We studied, in a large multicentre cohort, STI point prevalence, co-infection rates, anatomical location and influence of PrEP. Methods: The BRAHMS study was a prospective cohort study conducted at 10 sites in seven major German cities that enrolled MSM reporting increased sexual risk behaviour. At screening visits, MSM were tested for Mycoplasma genitalium (MG), Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT) and Treponema pallidum (TP), and given a behavioural questionnaire. With binomial regression, we estimated prevalence ratios (PR) and 95% confidence intervals (CI) for the association of PrEP and STI. Results: We screened 1,043 MSM in 2018 and 2019, with 53.0% currently using PrEP. At screening, 370 participants (35.5%) had an STI. The most common pathogen was MG in 198 (19.0%) participants, followed by CT (n = 133; 12.8%), NG (n = 105; 10.1%) and TP (n = 37; 3.5%). Among the 370 participants with at least one STI, 14.6% (n = 54) reported STI-related symptoms. Infection prevalence was highest at anorectal site (13.4% MG, 6.5% NG, 10.2% CT). PrEP use was not statistically significant in adjusted models for STI (PR: 1.10; 95% CI: 0.91–1.32), NG/CT, only NG or only CT. Conclusions: Prevalence of asymptomatic STI was high, and PrEP use did not influence STI prevalence in MSM eligible for PrEP according to national guidelines.Peer Reviewe

Fluid preinjection for microwave ablation in an ex vivo bovine liver model assessed with volumetry in an open MRI system

PURPOSEWe aimed to detect possible differences in microwave ablation (MWA) volumes after different fluid preinjections using magnetic resonance imaging (MRI).MATERIALS AND METHODSMWA volumes were created in 50 cuboid ex vivo bovine liver specimens (five series: control [no injection], 10 mL water, 10 mL 0.9% NaCl, 10 mL 6% NaCl, and 10 mL 12% NaCl preinjections; n=10 for each series). The operating frequency (915 megahertz), ablation time (7 min), and energy supply (45 watts) were constant. Following MWA, two MR sequences were acquired, and MR volumetry was performed for each sequence.RESULTSFor both sequences, fluid preinjection did not lead to significant differences in MWA ablation volumes compared to the respective control group (sequence 1: mean MWA volumes ranged from 7.0±1.2 mm [water] to 7.8±1.3 mm [12% NaCl] vs. 7.3±2.1 mm in the control group; sequence 2: mean MWA volumes ranged from 4.9±1.4 mm [12% NaCl] to 5.5±1.9 mm [0.9% NaCl] vs. 4.7±1.6 mm in the control group). The ablation volumes visualized with the two sequences differed significantly in general (P < 0.001) and between the respective groups (control, P ≤ 0.001; water, P < 0.001; 0.9% NaCl, P < 0.001; 6% NaCl, P ≤ 0.001; 12% NaCl, P < 0.001). The volumes determined with sequence 1 were closer to the expected ablation volume of 8 mL compared to those determined with sequence 2.CONCLUSIONFor the fluid qualities and concentrations assessed, there is no evidence that fluid preinjection results in larger coagulation volumes after MWA. Because ablation volumes determined by MRI vary with the sequence used, interventionalists should gain experience in how to interpret postinterventional imaging findings (with the MR scanner, sequences, and parameters used) to accurately estimate the outcome of the interventions they perform

Deep drilling reveals massive shifts in evolutionary dynamics after formation of ancient ecosystem

The scarcity of high-resolution empirical data directly tracking diversity over time limits our understanding of speciation and extinction dynamics and the drivers of rate changes. Here, we analyze a continuous species-level fossil record of endemic diatoms from ancient Lake Ohrid, along with environmental and climate indicator time series since lake formation 1.36 million years (Ma) ago. We show that speciation and extinction rates nearly simultaneously decreased in the environmentally dynamic phase after ecosystem formation and stabilized after deep-water conditions established in Lake Ohrid. As the lake deepens, we also see a switch in the macroevolutionary trade-off, resulting in a transition from a volatile assemblage of short-lived endemic species to a stable community of long-lived species. Our results emphasize the importance of the interplay between environmental/climate change, ecosystem stability, and environmental limits to diversity for diversification processes. The study also provides a new understanding of evolutionary dynamics in long-lived ecosystems