Synchronous fluorescence and multivariate classification analysis as a screening tool for determining Sudan I dye in culinary spices

Spices are a globally traded commodity which has been found to be adulterated with forbidden Sudan dyes. This work proposes a screening method for determining the adulteration of paprika varieties (mild, hot and smoked) with Sudan I dye, based on constant-wavelength synchronousfluorescence spectros-copy with multivariate classification. Different wavelength-intervals (Dl) were evaluated. Classification models were built with Partial Least Squares-Discriminant Analysis (PLS-DA) at two Sudan I dye con-centration levels (1 and 5 mg/L) and they were tested with samples at a lower level (0.5 mg/L). Classification results were quite satisfactory when a strategy based onfirst-derivative spectra was used for improving classification results. nm was chosen as the optimum wavelength interval giving a 100% of sensitivity and specificity. These results are promising because the risk of assigning adulterated samples as safe to be consumed is highly minimized. The proposed method is feasible, rapid and simple taking advantage of Sudan Ifluorescence phenomena in a direct way.Fil: Di Anibal, Carolina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Rodríguez, María Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Albertengo, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentin

Dietary Fiber and Availability of Nutrients: A Case Study on Yoghurt as a Food Model

Since the roles of dietary fibers in preventing and treating some diseases have been well documented, the addition of purified dietary fibers to foods has become more popular. Diffe rent types of dietary fibers (soluble and insoluble) and different sources (animal, plant, bacterial or fungi fibers) are now incorporated in purified form into foods for their nutritional or for their technological properties (e.g. gelling or thickening agents) resulting in healthy products. However, the intake of dietary fiber might affect the absorption of nutrients. This can be positive in certain pathologies (diabetes mellitus and cardiovascular diseases) or negative when nutritional deficiencies exist. There is a need to study the interactions between nutrients and dietary fiber and its influenc e on their absorption from f ood. Yogurt fortified with chitosan and different types of plants fibers like wheat, bamboo, apple, psyllium and inulin was used as a food model. The availabilities of glucose and calcium in this model were studied by an in vitro gastrointestinal tract simulation. The gastric environment was simulated with HCl (pH 1.0?2. 0). The duodenal environment was simulated with NaHCO3 (pH 6.8?7.2). Glucose was added to yoghurts with each type of fiber. To investigate the interaction of different fibers with iron from yoghurt, a dialysis tubing cellulose membrane was us ed in the digestive model to reproduce the duodenum wall. Ferrous sulfate was added to yoghurts with each type of fiber. Results showed that chitosan decreased glucose and calcium availabilities (17.7 ± 2.1% and 21.0 ± 2.5% depress respectively) and presented the highest iron retention percentages (53.2% at 30 min; 56.8% at 60 min). The effect of chitosan was higher than plants fibers used in this work.Fil: Dello Staffolo, Marina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Bevilacqua, Alicia Eva. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Rodriguez, Maria Susana. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Albertengo, Liliana. Universidad Nacional del Sur. Departamento de Química; Argentin

Synthesis, characterization and evaluation of reactional parameters on substitution degree of N-hexyl-N-methylene phosphonic chitosan

N-methylene phosphonic chitosan (NMPC) is treated with hexyl aldehyde to give an imine, which is easily converted into N-hexyl-N-methylene phosphonic chitosan (HNMPC) under mild conditions. The structure of this new chitosan derivative is characterized by FT- IR, 1H, 13C, 31P, 1H13C–HSQC NMR, SEM and XRD. The influence of reactional parameters on the substitution degree (DS), evidenced that a mol ratio 1.50:1.00 (hexyl aldehyde: free amino groups); a reaction time of 1 h. and 45 °C of temperature afford the best DS. HNMPC molecular weight is 12,768.62 Da. It shows good emulsifying properties giving o/w emulsions with high stability in time. Microscopic observation as well as particle size distribution show an unimodal droplet size distribution with low droplet diameters. Preliminary tests lead us to believe that this new polymer has good film forming properties.Fil: Zuñiga, Adriana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Forte Nerán, Romina Adela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Albertengo, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Rodriguez, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentin

Chitosan Interaction with Iron from Yoghurt Using an In Vitro Digestive Model: Comparative Study with Plant Dietary Fibers

The objective of this work was to investigate the interaction of chitosan with iron from yoghurt by an in vitro gastrointestinal tract model. Taking into account that chitosan is a polysaccharide included in fiber definition by Codex Alimentarius; chitosan behavior was studied and compared with different plant fiber (wheat, bamboo, apple, psyllium and inulin) behaviors, in the same in vitro conditions. Ferrous sulfate was added to yoghurts with each type of fiber. The gastric environment was simulated with HCl (pH 1.0–2.0). The duodenal environment was simulated with NaHCO3 (pH 6.8–7.2) and a dialysis tubing cellulose membrane. Results showed that chitosan had the highest iron retention percentages (53.2% at 30 min; 56.8% at 60 min) interacting in a more pronounced manner with iron than the plant fibers used in this work.Facultad de Ciencias Exacta

Assessment of the effectiveness of chitooligosaccharide from the Argentine sea resources to control Fusarium eumartii

El presente trabajo de investigación se refiere al estudio de la acción biológica del quitooligosacárido como fitosanitario de origen natural. Se analizó la acción protectora del oligómero en plántulas de tomate (Solanum lycopersicum cultivar Platense) inoculadas con el hongo patógeno Fusarium solani f sp eumartii (F. eumartii). Como modelo experimental se seleccionó un genotipo de tomate de interés hortícola en la provincia de Buenos Aires y un aislamiento fúngico de la misma región. Nuestros resultados demostraron que las plántulas de tomate pretratadas con quitoologosacárido fueron protegidas contra la infección por F. eumartii y registraron una disminución significativa en el área de la lesión fúngica detectada en los cotiledones. Además, se demostraron propiedades antimicrobianas del quitooligosacárido a través de la reducción del crecimiento micelial de F. eumartii y la germinación de esporas. La pérdida de viabilidad de las esporas ejercida por la acción del quitooligosacárido reveló su efecto fungicida. Asimismo, se comprobó el efecto del quitooligosacárido como inductor de la respuesta de defensa en las plantas de tomate. En las plantas tratadas con quitooligosacárido 0,025 % (m/v) durante 6 horas, se midió un aumento de aproximadamente 40 % en la abundancia de quitinasa, proteína marcadora de la respuesta de defensa inducida contra estrés fúngico. Finalmente, proponemos que el quitooligosacárido representa un compuesto natural que reúne actividades biológicas promisorias para su aplicación como agente antimicrobiano natural en el campo hortícola.Our specific aim was to study the properties ofchitooligosaccharide as a natural agent. The protectiveaction of chitooligosaccharide against the pathogenicfungus Fusarium solani f sp eumartii (F. eumartii) inseedlings of tomato (Solanum lycopersicum cultivarPlatense) was analyzed. We selected a tomato genotypeof horticultural interest in the Province of BuenosAires and a fungal isolated from the same region.Our results showed that tomato seedlings pretreatedwith chitooligosaccharide were protected againstinfection by F. eumartii, resulting in a significantdecrease in the area of the fungal lesion detected incotyledons. Moreover, antimicrobial properties by thechitooligosaccharide were demonstrated through areduction of the mycelial growth and spore germination.The loss of spore viability exerted by the action ofchitooligosaccharide revealed its fungicidal effect.Furthermore, the effect of the chitooligosaccharideas elicitor of the plant defense response was alsoproved. When plants were treated with 0.025% (m/v)chitooligosaccharide during 6 hours, an increase ofabout 40% in the abundance of chitinase as a defensemarker protein was measured. Finally, we proposethat the chitooligosaccharide represents a naturalcompound that brings promising biological activitiesfor its use as a natural antimicrobial agent in thehorticultural field.Fil: Mansilla, Andrea Yamila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Tonón, Claudia Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Albertengo, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; ArgentinaFil: Rodriguez, María Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; ArgentinaFil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentin

FAKTOR-FAKTOR YANG MEMPENGARUHI PERILAKU IBU DENGAN PEMBERIAN MP-ASI PADA ANAK USIA 6-24 BULAN DI WILAYAH KERJA PUSKESMAS KOPELMA DARUSSALAM BANDA ACEH

viKEMENTRIAN RISET, TEKNOLOGI DAN PENDIDIKAN TINGGIUNIVERSITAS SYIAH KUALAFAKULTAS KEPERAWATANSKRIPSI25 Juli 2016xvi halaman + VI bab + 79 Halaman + 14 Tabel + 1 Skema + 16 LampiranNURUL RACHMAH1207101020003FAKTOR-FAKTOR YANG MEMPENGARUHI PERILAKU IBU DENGANPEMBERIAN MP-ASI PADA ANAK USIA 6-24 BULAN DI WILAYAH KERJAPUSKESMAS KOPELMA DARUSSALAM BANDA ACEHABSTRAKMakanan Pendamping ASI merupakan makanan atau minuman bergizipendamping ASI yang mengandung karbohidrat, protein, lemak, vitamin danmineral. MP-ASI diberikan pada anak usia 6-24 bulan, tapi pada kenyataannyatetap saja banyak ibu yang memberikan MP-ASI secara tidak tepat, sepertimemberikan makanan pendamping ASI dini (sebelum usia 6 bulan), pemberianmakanan padat yang tidak sesuai dengan usia anak, dan memberikan MP-ASIdengan porsi yang terlalu berlebihan. Tujuan penelitian ini untuk mengetahuifaktor-faktor yang mempengaruhi perilaku ibu dengan pemberian MP-ASI padaanak (6-24 bulan) di wilayah kerja Puskesmas Kopelma Darussalam Banda Aceh.Penelitian ini menggunakan desain deskriptif korelatif. Sampel dalam penelitianini sebanyak 91 orang ibu yang dipilih dengan teknik proportional sampling.Waktu pengumpulan data dilakukan pada 23 Juni-3 Juli 2016, menggunakankuesioner dengan cara wawancara terpimpin, kemudian dianalisa menggunakanChi Square dengan nilai alpha=0,05. Hasil uji statistik diperoleh faktorpredisposisi (p-value=0,005), faktor pendukung (p-value=0,036) dan faktorpendorong (p-value= 0,001) yang menunjukkan hubungan dengan pemberian MPASI.Kesimpulan penelitian ini menunjukkan ada hubungan faktor-faktor yangmempengaruhi perilaku ibu (p-value=0,002) dengan pemberian MP-ASI padaanak usia (6-24 bulan) di wilayah kerja Puskesmas Kopelma Darussalam BandaAceh. Disarankan kepada tenaga kesehatan agar meningkatkan peran dalampenyuluhan tentang pentingnya pemberian MP-ASI, baik dalam programPosyandu, home visit, dan program-program kesehatan lainnya.Kata Kunci : Faktor perilaku, MP-ASI, ibu, anakDaftar Bacaan : 22 Buku, 6 jurnal, 8 situs internet, 2 Skripsi (2001-2016

Chitosan as Source for Pesticide Formulations

Late blight and wilt caused by the oomycete, Phytophthora infestans, and the fungus, Fusarium solani f. sp. eumartii, respectively, are severe diseases in Solanaceae crops worldwide. Although traditional approaches to control plant diseases have mainly relied on toxic chemical compounds, current studies are focused to identify more sustainable options. Finding alternatives, a low molecular weight chitosan (LMWCh) obtained from biomass of Argentine Sea’s crustaceans was assayed. In an attempt to characterize the action of LMWCh alone or in combination with the synthetic fungicide Mancozeb, the antimicrobial properties of LMWCh were assayed. In a side-by-side comparison with the SYTOX Green nucleic acid stain and the nitric oxide–specific probe, diaminofluorescein-FM diacetate (DAF-FM DA), yielded a similar tendency, revealing LMWCh-mediated cell death. The efficacy of LMWCh, Mancozeb, and the mixture LMWCh–Mancozeb was in turn tested. A synergistic effect in the reduction of F. eumartii spore germination was measured in the presence of subinhibitory dosis of 0.025 mg ml−1 LMWCh and 0.008 mg ml−1 Mancozeb. This mixture was efficient to increase the effectiveness of the single treatments in protecting against biotic stress judged by a drastic reduction of lesion area in P. infestans–inoculated tissues and activation of the potato defense responses

Chitosan Interaction with Iron from Yoghurt Using an In Vitro Digestive Model: Comparative Study with Plant Dietary Fibers

The objective of this work was to investigate the interaction of chitosan with iron from yoghurt by an in vitro gastrointestinal tract model. Taking into account that chitosan is a polysaccharide included in fiber definition by Codex Alimentarius; chitosan behavior was studied and compared with different plant fiber (wheat, bamboo, apple, psyllium and inulin) behaviors, in the same in vitro conditions. Ferrous sulfate was added to yoghurts with each type of fiber. The gastric environment was simulated with HCl (pH 1.0–2.0). The duodenal environment was simulated with NaHCO3 (pH 6.8–7.2) and a dialysis tubing cellulose membrane. Results showed that chitosan had the highest iron retention percentages (53.2% at 30 min; 56.8% at 60 min) interacting in a more pronounced manner with iron than the plant fibers used in this work.Facultad de Ciencias Exacta

Educación cooperativa : experiencias escolares significativas

Fil: Ferreyra, Horacio Ademar. Universidad Católica de Córdoba. Facultad de Educación; Argentin

Educación cooperativa: experiencias escolares significativas

Fil: Ferreyra, Horacio Ademar. Universidad Católica de Córdoba. Facultad de Educación; Argentin