11 research outputs found
Oral lesions in HIV infected individuals from RibeirĂŁo Preto, Brazil
Objectives: The aim of this study was to diagnosis oral lesions related to HIV infection in individuals followed in the General Hospital of the School of Medicine of RibeirĂŁo Preto, University of SĂŁo Paulo, Brazil. The presence of oral lesions was correlated with gender, age, smoking habit, levels of CD4 lymphocytes, HIV load, time of HIV seropositivity, AIDS condition, use of removable dental prosthesis, and use of HAART. Materials and Methods: 340 HIV infected individuals were selected for this study, all participants of the study were examined by only one practiced dentist which performed anamnesis, peribuccal and oral examination. Results: Oral lesions were observed in 113 of 340 (33.2%) HIV infected individuals. These oral lesions included: oral candidiasis (17.7%) of pseudomembranous (10.8%) and of erythematous types (6.9%), angular cheilitis (13.9%), hairy leukoplakia (11.8%), and oral ulcers (2.1%). Oral candidiasis lesions were more frequently observed in women (p.033). Smoking addict participants presented a high frequency of tongue hairy leukoplakia (p.038) and a reduced frequency of oral ulcers (p.018). Hairy leukoplakia and pseudomembranous candidiasis were inversely correlated to CD4+L levels and directly correlated with HIV load, behaving as immune depression markers. Hairy leukoplakia and pseudomembranous candidiasis also showed an inverse correlation with HAART use (p<.0001). Patients using mobile dental prosthesis presented a high frequency of erythematous candidiasis (p.003). Conclusion: The inverse correlation with CD4+L level and the direct correlation with HIV load suggest that oral lesions could be used as alternative clinical markers for poor immune condition in HIV infected individuals
Oral Candida spp carriage and periodontal diseases in HIV-infected patients in RibeirĂŁo Preto, Brazil
The majority of HIV-infected patients develop Candida spp-associated clinical oral lesions. Studies have shown that asymptomatic oral colonization of Candida spp may lead to oral lesions or become a source of disseminated infections. The aim of this study was to verify the effects of periodontal conditions on Candida spp prevalence and Candida spp carriage in the oral cavity of HIV-infected patients compared to non-infected patients. Twenty-five patients not infected with HIV and 48 HIV-infected patients were classified according to periodontal conditions as being periodontal healthy or with periodontal disease. Candida spp carriage and classification were performed in oral rinse samples. Viral load and CD4+ T lymphocyte (CD4+L) counts were performed in blood samples from HIV-infected patients. No differences in Candida spp prevalence related to HIV status or periodontal condition were detected. However, Candida spp carriage was increased in periodontally affected HIV-infected patients when compared to periodontally healthy HIV-infected patients (p= 0.04). Periodontally healthy HIV-infected patients presented Candida spp carriage in similar levels as healthy or periodontally affected non-HIV-infected patients. Candida spp carriage was correlated with CD4+L counting in HIV-infected patients. We concluded that periodontal disease is associated with increased Candida spp carriage in HIV-infected patients and may be a predisposing factor to clinical manifestations of candidiasis
Oral health management of 97 patients living with HIV/AIDS in RibeirĂŁo Preto, SĂŁo Paulo, Brazil
Estudo in vitro da capacidade da lactoferrina e de seu produto de clivagem (G-12-I), presentes nas secreçÔes orais e genitais, de modular a produção de CCL20 pelas células do epitélio endocervical : envolvimento com a transmissão sexual do HIV
Dendritic cells, among them Langerhans cells, are recognized as presenters of Human Immunodeficiency Virus (HIV) to CD4 + T lymphocytes. The recruitment of Langerhans cells in the vaginal mucosa is modulated by Chemokine CC motif ligand 20 (CCL20) produced by epithelial cells of the female genital mucosa. The aim of this study was to evaluated the capacity of seminal plasma, saliva and vaginal secretions to induce the production of CCL20 by monostratified endocervical epithelium cells culture (HEC-1A), also, the relation of this production with the presence of lactoferrin and its cleavage products (G- 12-I) in seminal plasma and saliva. Cultures of HEC-1A cells were stimulated with seminal plasma, saliva and vaginal secretions of HIV-seronegative and HIV-seropositive participants, and the CCL20 production measured by enzyme linked immunosorbent assay (ELISA) in its cellular supernatant. ELISA for Lf was performed on all samples, and the peptide cleavage Lf (G-12-I) observed in the samples which more and less induced the CCL20 secretion on western blot. We observed that the seminal plasma of seropositive participants were responsible for a higher stimulation of CCL20 production by HEC-1A cells, when compared to the seminal plasma of HIV-seronegative participants. Lf concentration in seminal plasma was positively related to the CCL20 production by HEC-1A cells, the higher production of CCL20 was also more associated with Lf peptide cleavage Lf (G-12-I), on western blot. Saliva stimulated CCL20 production by HEC-1A cells, and such stimulation was not correlated to Lf concentration. In conclusion, the presence of Lf in seminal plasma positively related to the production of CCL20 by HEC-1A cells, and saliva may induce the production of CCL20 in the female genital mucosaLes cellules dendritiques prĂ©sentes dans les muqueuses comme les cellules de Langerhans, sont capables de prĂ©senter le Virus d'ImmunodĂ©ficience Humaine (VIH) aux lymphocytes T CD4+, aboutissant Ă l'infection par le VIH lors de contacts hĂ©tĂ©rosexuels non protĂ©gĂ©s. Le recrutement des cellules de Langerhans dans la muqueuse vaginale est assurĂ© par la chimiokine Quimiocin C-C motif ligant 20 (CCL20) produite par les cellules Ă©pithĂ©liales de la muqueuse. L'objectif de ce travail a Ă©tĂ© dâĂ©tudier la capacitĂ© du plasma sĂ©minal, de la salive et de la sĂ©crĂ©tion vaginale Ă stimuler la production de CCL20 par les cellules de lâĂ©pithĂ©lium endocervical (cellules HEC-1A), et de corrĂ©ler cette production avec la prĂ©sence de la lactoferrine (LF) et de ses produits de clivage dans ces diffĂ©rents fluides biologiques. Des cellules HEC-1A ont Ă©tĂ© cultivĂ©es avec les plasmas sĂ©minaux, les salives et les sĂ©crĂ©tions vaginales de patients sĂ©ronĂ©gatifs et sĂ©ropositifs pour le VIH, et la production de CCL20 a Ă©tĂ© mesurĂ©e dans les surnageants cellulaires en ELISA. Le dosage de la Lf par ELISA a Ă©tĂ© rĂ©alisĂ© sur tous les Ă©chantillons. La prĂ©sence dâun petide de clivage de la Lf (G-12-I) a Ă©tĂ© analysĂ©e en western blot. Les rĂ©sultats obtenus montrent que les plasmas sĂ©minaux des patients sĂ©ropositifs ont une plus grande capacitĂ© Ă stimuler la sĂ©crĂ©tion de CCL20 que les patients sĂ©ronĂ©gatifs pour le VIH. La concentration de la Lf dans le plasma sĂ©minal est corrĂ©lĂ©e Ă la stimulation de cette sĂ©crĂ©tion par les cellules HEC-1A. Il semble que les Ă©chantillons ayant la plus grande capacitĂ© Ă stimuler la sĂ©crĂ©tion de CCL20 possĂšdent une plus forte concentration en petide de clivage de la Lf (G-12-I), bien que ce rĂ©sultat, obtenu en western blot. La salive est Ă©galement capable de stimuler de la production de CCL20 par les cellules HEC-1A, mais cette stimulation n'est pas corrĂ©lĂ©e avec sa concentration en Lf. Nous concluons que la sĂ©crĂ©tion de CCL20 par lâĂ©pithĂ©lium gĂ©nital fĂ©minin induite par le plasma sĂ©minal est corrĂ©lĂ©e Ă la prĂ©sence de la Lf dans ce fluide. Bien que les mĂ©canismes puissent ĂȘtre diffĂ©rents, la salive peut Ă©galement induire la production de CCL20 par les cellules Ă©pithĂ©liales de la muqueuse gĂ©nitale fĂ©minineAs cĂ©lulas dentrĂticas, dentre elas as cĂ©lulas de Langerhans, sĂŁo capazes de apresentar o vĂrus da imunodeficiĂȘncia humana (HIV) aos linfĂłcitos T CD4+, cujo processo culmina com a infecção por esse vĂrus. O recrutamento das cĂ©lulas de Langerhans na mucosa vaginal Ă© modulada pela Quimiocin C-C motif ligant 20 (CCL20), produzida pelas cĂ©lulas epiteliais da mucosa genital feminina. O objetivo desse trabalho foi estudar a capacidade do plasma seminal, da saliva e do conteĂșdo vaginal de induzir a produção de CCL20 pela cultura de cĂ©lulas do epitĂ©lio monoestratificado endocervical (HEC-1A), relacionando tal produção com a presença da lactoferrina (Lf) e de seu produto de clivagem (G-12-I) nestes fluidos. Culturas de cĂ©lulas HEC-1A foram estimuladas com plasmas seminais, salivas e conteĂșdos vaginais de participantes soronegativos e soropositivos para o HIV, visando avaliar a produção de CCL20, a qual foi mensurada utilizando-se o Enzyme Linked Immunosorbent Assay (ELISA). A dosagem de Lf (ELISA) foi realizada em todas as amostras estudadas e a presença do peptĂdeo de clivagem da Lf (G-12-I) observada nas amostras que mais e que menos induziram a secreção de CCL20 pelas cĂ©lulas HEC-1A por Western blot. Verificou-se que os plasmas seminais de participantes soropositivos foram responsĂĄveis por maior estimulação da produção de CCL20 pelas cĂ©lulas HEC-1A, quando comparados aos plasmas seminais de participantes soronegativos para o HIV. A concentração de Lf no plasma seminal associou-se Ă indução na produção de CCL20 pelas cĂ©lulas HEC-1A, assim como as amostras que mais induziram a produção de CCL20 apresentaram presenças mais evidentes do peptĂdeo de clivagem da Lf (G-12-I). A saliva foi responsĂĄvel pela estimulação na produção de CCL20 pelas cĂ©lulas HEC-1A, no entanto, tal estimulação nĂŁo se associou Ă concentração de Lf salivar. ConcluĂmos que a presença de Lf no plasma seminal esteve correlacionada Ă produção de CCL20 pelas cĂ©lulas HEC-1A, e que a saliva pode induzir a produção da CCL20 pelas cĂ©lulas epiteliais endocervicai
Ătude in vitro de la capacitĂ© de la lactoferrine et son produit de clivage (G-12-I), prĂ©sent dans les sĂ©crĂ©tions orales et gĂ©nitales, de moduler la production de CCL20 par les cellules Ă©pithĂ©liales endocervicales : implication dans la transmission sexuelle du VIH
Les cellules dendritiques prĂ©sentes dans les muqueuses comme les cellules de Langerhans, sont capables de prĂ©senter le Virus d'ImmunodĂ©ficience Humaine (VIH) aux lymphocytes T CD4+, aboutissant Ă l'infection par le VIH lors de contacts hĂ©tĂ©rosexuels non protĂ©gĂ©s. Le recrutement des cellules de Langerhans dans la muqueuse vaginale est assurĂ© par la chimiokine Quimiocin C-C motif ligant 20 (CCL20) produite par les cellules Ă©pithĂ©liales de la muqueuse. L'objectif de ce travail a Ă©tĂ© dâĂ©tudier la capacitĂ© du plasma sĂ©minal, de la salive et de la sĂ©crĂ©tion vaginale Ă stimuler la production de CCL20 par les cellules de lâĂ©pithĂ©lium endocervical (cellules HEC-1A), et de corrĂ©ler cette production avec la prĂ©sence de la lactoferrine (LF) et de ses produits de clivage dans ces diffĂ©rents fluides biologiques. Des cellules HEC-1A ont Ă©tĂ© cultivĂ©es avec les plasmas sĂ©minaux, les salives et les sĂ©crĂ©tions vaginales de patients sĂ©ronĂ©gatifs et sĂ©ropositifs pour le VIH, et la production de CCL20 a Ă©tĂ© mesurĂ©e dans les surnageants cellulaires en ELISA. Le dosage de la Lf par ELISA a Ă©tĂ© rĂ©alisĂ© sur tous les Ă©chantillons. La prĂ©sence dâun petide de clivage de la Lf (G-12-I) a Ă©tĂ© analysĂ©e en western blot. Les rĂ©sultats obtenus montrent que les plasmas sĂ©minaux des patients sĂ©ropositifs ont une plus grande capacitĂ© Ă stimuler la sĂ©crĂ©tion de CCL20 que les patients sĂ©ronĂ©gatifs pour le VIH. La concentration de la Lf dans le plasma sĂ©minal est corrĂ©lĂ©e Ă la stimulation de cette sĂ©crĂ©tion par les cellules HEC-1A. Il semble que les Ă©chantillons ayant la plus grande capacitĂ© Ă stimuler la sĂ©crĂ©tion de CCL20 possĂšdent une plus forte concentration en petide de clivage de la Lf (G-12-I), bien que ce rĂ©sultat, obtenu en western blot. La salive est Ă©galement capable de stimuler de la production de CCL20 par les cellules HEC-1A, mais cette stimulation n'est pas corrĂ©lĂ©e avec sa concentration en Lf. Nous concluons que la sĂ©crĂ©tion de CCL20 par lâĂ©pithĂ©lium gĂ©nital fĂ©minin induite par le plasma sĂ©minal est corrĂ©lĂ©e Ă la prĂ©sence de la Lf dans ce fluide. Bien que les mĂ©canismes puissent ĂȘtre diffĂ©rents, la salive peut Ă©galement induire la production de CCL20 par les cellules Ă©pithĂ©liales de la muqueuse gĂ©nitale fĂ©minineDendritic cells, among them Langerhans cells, are recognized as presenters of Human Immunodeficiency Virus (HIV) to CD4 + T lymphocytes. The recruitment of Langerhans cells in the vaginal mucosa is modulated by Chemokine CC motif ligand 20 (CCL20) produced by epithelial cells of the female genital mucosa. The aim of this study was to evaluated the capacity of seminal plasma, saliva and vaginal secretions to induce the production of CCL20 by monostratified endocervical epithelium cells culture (HEC-1A), also, the relation of this production with the presence of lactoferrin and its cleavage products (G- 12-I) in seminal plasma and saliva. Cultures of HEC-1A cells were stimulated with seminal plasma, saliva and vaginal secretions of HIV-seronegative and HIV-seropositive participants, and the CCL20 production measured by enzyme linked immunosorbent assay (ELISA) in its cellular supernatant. ELISA for Lf was performed on all samples, and the peptide cleavage Lf (G-12-I) observed in the samples which more and less induced the CCL20 secretion on western blot. We observed that the seminal plasma of seropositive participants were responsible for a higher stimulation of CCL20 production by HEC-1A cells, when compared to the seminal plasma of HIV-seronegative participants. Lf concentration in seminal plasma was positively related to the CCL20 production by HEC-1A cells, the higher production of CCL20 was also more associated with Lf peptide cleavage Lf (G-12-I), on western blot. Saliva stimulated CCL20 production by HEC-1A cells, and such stimulation was not correlated to Lf concentration. In conclusion, the presence of Lf in seminal plasma positively related to the production of CCL20 by HEC-1A cells, and saliva may induce the production of CCL20 in the female genital mucosaAs cĂ©lulas dentrĂticas, dentre elas as cĂ©lulas de Langerhans, sĂŁo capazes de apresentar o vĂrus da imunodeficiĂȘncia humana (HIV) aos linfĂłcitos T CD4+, cujo processo culmina com a infecção por esse vĂrus. O recrutamento das cĂ©lulas de Langerhans na mucosa vaginal Ă© modulada pela Quimiocin C-C motif ligant 20 (CCL20), produzida pelas cĂ©lulas epiteliais da mucosa genital feminina. O objetivo desse trabalho foi estudar a capacidade do plasma seminal, da saliva e do conteĂșdo vaginal de induzir a produção de CCL20 pela cultura de cĂ©lulas do epitĂ©lio monoestratificado endocervical (HEC-1A), relacionando tal produção com a presença da lactoferrina (Lf) e de seu produto de clivagem (G-12-I) nestes fluidos. Culturas de cĂ©lulas HEC-1A foram estimuladas com plasmas seminais, salivas e conteĂșdos vaginais de participantes soronegativos e soropositivos para o HIV, visando avaliar a produção de CCL20, a qual foi mensurada utilizando-se o Enzyme Linked Immunosorbent Assay (ELISA). A dosagem de Lf (ELISA) foi realizada em todas as amostras estudadas e a presença do peptĂdeo de clivagem da Lf (G-12-I) observada nas amostras que mais e que menos induziram a secreção de CCL20 pelas cĂ©lulas HEC-1A por Western blot. Verificou-se que os plasmas seminais de participantes soropositivos foram responsĂĄveis por maior estimulação da produção de CCL20 pelas cĂ©lulas HEC-1A, quando comparados aos plasmas seminais de participantes soronegativos para o HIV. A concentração de Lf no plasma seminal associou-se Ă indução na produção de CCL20 pelas cĂ©lulas HEC-1A, assim como as amostras que mais induziram a produção de CCL20 apresentaram presenças mais evidentes do peptĂdeo de clivagem da Lf (G-12-I). A saliva foi responsĂĄvel pela estimulação na produção de CCL20 pelas cĂ©lulas HEC-1A, no entanto, tal estimulação nĂŁo se associou Ă concentração de Lf salivar. ConcluĂmos que a presença de Lf no plasma seminal esteve correlacionada Ă produção de CCL20 pelas cĂ©lulas HEC-1A, e que a saliva pode induzir a produção da CCL20 pelas cĂ©lulas epiteliais endocervicai
Oral health management of 97 patients living with HIV/AIDS in RibeirĂŁo Preto, SĂŁo Paulo, Brazil
Considering the changes antiretroviral therapy (ART) has brought to the treatment of HIV infection, the current clinical and laboratory profiles of HIV/AIDS individuals referred to oral health centers are crucially important in instructing dentists about the oral health management of these patients. The aim of the present study was to determine the clinical and laboratory profiles of HIV-infected individuals referred to a clinic for patients with special needs between 2005 and 2012 by retrospectively analyzing their dental records. A total of 97 records of HIV patients referred to the School of Dentistry of RibeirĂŁo Preto, Universidade de SĂŁo Paulo - USP, were analyzed. The Mann-Whitney test was used to determine the associations between mean CD4+ counts, mean viral load, and the presence of HIV-related oral lesions (HIV-OL). Most of the patients were male, and their mean age was 38.3 years. Eighty-nine (92%) patients were on regular ART, 77 (79.4%) had a CD4+ count higher than 200 cells/mm3, and 63 (64.9%) had an undetectable viral load. Twenty patients (20.6%) presented with some HIV-OL, including pseudomembranous and/or erythematous candidiasis and angular cheilitis, which were correlated with a low CD4+ count and with an undetectable viral load (p < 0.05). Among the branches of dentistry, periodontics, followed by surgery and restorative dentistry, was the most sought-after specialty, and no intercurrent events were observed during the dental treatment. It may be concluded that there are no restrictions on the dental treatment of patients on regular ART, It is important, though, that the treatment be based on local characteristics and on the prevention of oral diseases.</p