Genetic relationship of wild einkorn based on geographical distribution in Anatolia and Thrace using AFLP markers

Triticum monococcum L. ssp boeoticum Boiss., is the wild progenitor of domesticated einkorn. High throughput AFLP genetic analysis showed that the domestication of einkorn started in the northern part of the Fertile Crescent, near the Karacadag Mountains, Southeastern Turkey [1]. This study assesses the genetic distribution and the diversity of wild einkorn throughout Turkey, using total of 59 accessions from 22 locations in four different geographical regions. In our study, the four selective combinations of AFLP markers (E+ACC/M+ACT, E+ACC/M+ATA, E+ACT/M+ATA, and E+ATC/M+AAG) resulted in 161 AFLP marker loci. Phylogenetic trees for individual accessions and populations based on geographical regions were obtained using 'PopGen-32' population genetic analysis software. East and Southeast samples were genetically closest to each other among the samples from other regions. The samples from West, Northwest, and Central Anatolia were clustered together

Identification of differentially expressed proteins in wheat after benzothiadiazole treatment

The systemic acquired resistance (SAR) mechanism is stimulated by biological and chemical agents in response to pathogen infection as a part of the innate immunity response system of plants. The externally applied synthetic chemical, benzo-(1,2,3)-thiadiazole-7-carbothioic acid-S-methyl ester (BTH), is also known to induce a SAR response in plants. Studies identifying genes induced or suppressed by BTH are limited. Only few genes responding to BTH are determined. The focus of this preliminary study is to identify the gene products affected by BTH in wheat. A 2D-polyacrylamide gel electrophoresis (PAGE) analysis was carried out with BTH treated Triticum aestivum cv. Gerek-79 and mock treated samples. Following 2D-PAGE image analysis, the selected differentially expressed protein spots were identified by nanoLC-ESI-MS/MS. Among the 26 protein spots distinguished, five were found to be increased upon BTH treatment, another set of seven spots were absent in the control sample.Thus, they were apparent only in the gel of the BTH treated sample (+BTH), whereas five protein spots disappeared in the gel of the BTH treated plants (-BTH). Up-reg-ulation of some proteins such as OEE2 (oxygen evolving enhancer protein) and COR (cold-responsive) LEA (late em-bryogenesis abundant)/RAB (responsive to abscisic acid, ABA) and down-regulation of some proteins such as RuBisCo LSU (large subunit), fructose 1, 6-biphosphate aldolase (AldP), methyl binding domain protein 6 (MBD6), and 3-isopropyl-malate dehydrogenase are shown for the first time in BTH treatment of wheat. © Eugen Ulmer KG, Stuttgart

The effect of nebivolol on P wave duration and dispersion in patients with Behçet's disease; a prospective single-arm controlled study [Behçet hastali{dotless}gi{dotless}nda nebivololün P dalga süresi ve P dalga dispersiyonuna etkisi; prospektif, tek grup kontrollü çali{dotless}şma]

PubMed ID: 23996803Objective: Behçet's disease (BD) is a chronic multi-system disease presenting with recurrent oral and genital ulceration, and relapsing uveitis. Left ventricular diastolic dysfunction, ventricular arrhythmia and sudden cardiac death have been documented in BD. P wave dispersion (PD) has been reported to be associated with inhomogeneous and discontinuous propagation of sinus impulses. PD has been reported to be longer in patients with BD. Nebivolol, besides its selective beta1-blocking activity, causes an endothelium dependent vasodilatation through nitric oxide release. In this study, we searched for the effects of nebivolol on P wave duration and dispersion in patients with BD. Methods: This study was designed as prospective single-arm controlled study. We prospectively studied 35 Behçet's patients who were diagnosed according to the International Study Group criteria. Patients received 5 mg nebivolol per day for 3 months. The patients were evaluated with 12-leads electrocardiography at baseline and after for 3-month therapy. The difference between maximum and minimum P wave durations was defined as PD. The paired samples t test, Wilcoxon test were used for statistical analysis. Results: A significant decrease was observed in PD after therapy period (62.85±21.62 vs. 44.28±18.03 msec, p=0.001). No adverse effects were observed in treatment period. Conclusion: BD is associated with prolonged P wave duration and dispersion. We have shown for the first time that nebivolol causes a significant decrease in maximum P wave duration and PD in patients with BD. However, further comprehensive studies are needed to determine the long-term effects of nebivolol. © 2013 by AVES Yayi{dotless}nci{dotless}li{dotless}k Ltd

An Integrated Genetic Linkage Map of the Soybean Genome

A number of molecular genetic maps of the soybean [Glycine max (L.) Merr.] have been developed over the past 10 yr. These maps are primarily based on restriction fragment length polymorphism (RFLP) markers. Parental surveys have shown that most RFLP loci have only two known alleles. However, because the soybean is an ancient polyploid, RFLP probes typically hybridize and map to more than one position in the genome. Thus, the polymorphic potential of an RFLP probe is primarily a function of the frequency of the two alleles at each locus the probe detects. In contrast, simple sequence repeat (SSR) markers are single locus markers with multiple alleles. The polymorphic potential of an SSR marker is dependent on the number of alleles and their frequencies. Single locus markers provide an unam- biguous means of defining linkage group homology across mapping populations. The objective of the work reported here was to develop and map a large set of SSR markers. A total of 606 SSR loci were mapped in one or more of three populations: the USDA/Iowa State G. max x G. soja F 2 population, the Univ. of Utah Minsoy x Noir 1 recombinant inbred population, and the Univ. of Nebraska Clark x Harosoy F2 population. Each SSR mapped to a single locus in the genome, with a map order that was essentially identical in all three populations. Many SSR loci were segregating in two or all three populations. Thus, it was relatively simple to align the 201 linkage groups derived from each of the three populations into a consensus set of 20 homologous linkage groups presumed to correspond to the 20 pairs of soybean chromosomes. On the basis of in situ segregation or linkage reports in the literature all but one of the classical linkage groups can now be assigned to a corresponding molecular linkage group

The Effect of Education on the Early Diagnosis of Breast and Cervix Cancer on the Women’s Attitudes and Behaviors Regarding Participating in Screening Programs

The objective of this paper was to define the effect of education on the early diagnosis of breast and cervix cancer on the women's attitudes and behaviors regarding participating in Cancer Early Diagnosis, Screening and Training Centers-CEDSTC screening programs. This semi-experimental study was completed with 342 women. The data were collected with forms “Champion’s Health Belief Model Scale Breast Cancer-HBMSBC” and “Health Belief Model Scale for Cervical Cancer and the Pap Smear Test-HBMSCCPST.” When the women’s health beliefs before and after 6 months of the education about the early diagnosis of breast and cervical cancers are considered, it is seen that the HBMSBC subscales health motivation, breast self-examination (BSE), and evasion to mammography (MMG) decreased and BSE self-efficacy and MMG benefit attitudes increased and HBMSCCPST subscales pap smear benefit attitudes increased and evasion to pap smear attitude decreased (p < 0.05). Six months after the education, 28.4% of the women had undergone MMG, 69.9% had performed BSE, and 33.6% had undergone a pap smear test. Education regarding early diagnosis of breast and cervix cancer was found to have positive effects on the health behaviors of the women related to BSE, MMG, and pap smear tests. The women require professional education program for increasing their attitudes and behaviors for CEDSTC screening programs. We suggest regularly providing education to increase participation in early screening programs. © 2017, American Association for Cancer Education.Firat University Scientific Research Projects Management UnitThe authors are grateful to all the participants in the study. We offer our heartfelt thanks to the Provincial Public Health Director of that period who gave support and an official vehicle and staff while visiting the villages and towns of Ni?de Province center when conducting this study. We want to state that we are thankful to all midwives, nurses, and officials working at Ni?de CEDSTC who supported the implementation of our project for their help and to Ni?de University Z?beyde Han?m School of Health Department of Nursing and Midwifery students who helped collect the data. This study was presented as an oral presentation at the First International Congress on Women and Child Health and Training on 14?15 April 2016 in Kocaeli, Turkey. This study was presented as oral presentation at First International Congress on Women and Child Health and Training, 14?15 April 2016, Kocaeli, Turkey The authors declare that they have no conflicts of interest. The author(s) disclosed receipt of the following financial support for the research and/or authorship of this article. This research was supported by Ni?de University Scientific Research Project (Project no: SSB2012/01)