Seleksi Isolat Bakteri dan Optimasi Konsentrasi Awal Substrat Gliserol dalam Fermentasi Etanol

Tingginya jumlah gliserol sebagai produk dari berbagai industri mensyaratkan adanya upaya pengolahan gliserol menjadi produk lain yang bernilai ekonomis. Pada penelitian ini dilakukan seleksi kemampuan beberapa isolat bakteri dalam mengkonversi gliserol menjadi etanol.Isolat yang diuji berupa isolat bakteri dari rumen sapi dan isolat dari koleksi kultur Laboratorium Mikrobiologi SITH ITB yaitu, Enterobacter aerogenes, Bacillus licheniformis, B. subtilis, B. circulans, dan B. cereus. Hasil penapisan menunjukkan terbentuknya etanol pada medium basal yang mengandung 0,5% (b/v) gliserol yang diinokulasi dengan E. aerogenes, B. licheniformis, dan B. subtilis, sedangkanisolat dari isi rumen sapi menunjukkan hasil yang negatif. Pada kondisi anaerob E. aerogenes menghasilkan kadar etanol tertinggi sebesar 0,033 g/L dengan laju pembentukan etanol rata-rata sebesar 0,006 g/L/hari dan laju pertumbuhan rata-rata sebesar 0,0741/hari. Selanjutnya, kadar etanol tertinggi sebesar 0,036 g/L dicapai pada variasi kadar awal gliserol 1% (b/v) dengan laju pembentukan etanol rata-rata sebesar 0,008 g/L/hari dan laju pertumbuhan rata-rata sebesar 0,111/hari. Pada kondisi optimum (berdasarkan hasil-hasil percobaan sebelumnya) diperoleh etanol dengan kadar tertinggi 0,04 g/L yang setelah dikonfirmasi dengan analisis GC kadar etanol adalah sebesar 0,791 g/L

Isolasi Dan Karakterisasi Bakteri Penghasil Biogum Xanthan Dari Brassica Oleraceae Asal Kepunan Magelang Jawa Tengah

Food additive such as gelling agent and emulsifier is essential ingredient in food processing. Microbial gum such as xanthan gum is a hydrocolloid microbial that could play as gelling agents and emulsifier in food industry. Xanthan gum is produced by a phytopathogenic bacteria, Xanthomonas campestris. This study aims to isolate xanthan gum producing local bacteria of Brassica oleracea L. leaf showing black root symptom. The isolation performed by inoculating leaf an nutrient agar media. Three isolated bacteria were purified and tested their ability in producing gum using YDC broth for 4 days. The test result showed that the isolates were Xh.A, Xh.B, and Xh.C had ability to produce biogum. Profile matching resul t showed that Xh.A, Xh.b, and Xh.C were identified as member of Pseudomonas, Aureobacterium and Xanthomonas genus, respectively

Cost Estimation of Road Traffic Injuries Among Iranian Motorcyclists Using the Willingness to Pay Method

Background: Motorcycle riders are amongst some of the most vulnerable road users. The burden of motorcycles injuries from low and middle income countries is under-reported. Objectives: In this study, the cost of traffic injuries among motorcyclists was calculated using the willingness to pay (WTP) method in Iran in 2013. Patients and Methods: In a cross-sectional study, 143 motorcyclists were randomly selected. The research questionnaire was prepared based on the standard WTP method [stated preference (SP), contingent value (CV) and revealed preference (RP) models] taking into consideration perceived risks, especially those in Iran. Data were collected by a scenario for motorcyclists. The criteria for inclusion in the study consisted of having at least a high school education and being in the age range of 18 - 65 years. The final analysis of the WTP data was performed using the Weibull model. Results: The mean WTP was 888,110 IRR (Iranian Rial) among motorcyclists. The statistical value of life was estimated according to 4694 death cases as 3,146,225,350,943 IRR, which was equivalent to USD 104,874,178 based on the dollar free market rate of 30,000 IRR (purchasing power parity). The cost of injury was 6,903,839,551,000 IRR, equivalent to USD 230,127,985 (based upon 73,325 injured motorcyclists in 2013, a daily traffic volume of 311, and a daily payment of 12,110 IRR for 250 working days). In total, injury and death cases came to 10,050,094,901,943 IRR, equivalent to USD 335,003,163. Willingness to pay had a significant relationship with having experienced an accident, the length of the daily trip (in km), and helmet use (P < 0.001). Conclusions: Willingness to pay can be affected by experiencing an accident, the distance of the daily trip, and helmet use. The cost of traffic injuries among motorcyclists shows that this rate is much higher than the global average. Thus, expenditure should be made on effective initiatives such as the safety of motorcyclists

Determine Differences in Adolescents Sexual Behaviour Between Students of SMA X and SMA Y Indralaya

Background : For someone life, adolescence has an important meaning to gain personal character that give great contribution for their life in the future. Adolescent sexual behaviour can be manifested in various acts, such as attracted feeling to opposite sex, desire for dating, holding hands each other, desire to hug, kissing, untill intercourse act. This study aims to determine differences in adolescents sexual behaviour between students of SMA X and SMA Y Indralaya in 2010. Method : This research is quantitative research with cross sectional approach. the methode is used by spreading queationnaires to students. Data is collected and processed using SPSS program and performed by univariate and bivariate analysis. Sampels are 100 students of SMA X and 60 students of SMA Y. Result : The results show that based not there are difference which knowledge significant, attitude, guidance ad counseling, and role of peers between students of SMA X and SMA Y. Conclusion : There is difference which is signifikan on parental role between students of SMA X and SMA Y, while adolescent sexual behavior at students of SMA X and SMA Y is same

Effect of fennel (Foeniculum vulgare Mill.) and coriander (Coriandrum sativum L.) on microbial quality and sensory acceptability of frozen paratha

Fennel (Foeniculum vulgare Mill.) and coriander (Coriandrum sativum L.) are known to possess good antimicrobial properties. In the present work, spice-infused frozen parathas were formulated to investigate the effect of fennel and coriander on microbial (aerobic mesophilic bacteria, yeast and mould, and Bacillus cereus) reduction and sensory acceptability of frozen paratha throughout the storage at -18°C. The present work was also aimed at determining the relationship between spice concentrations and storage durations on microbiological quality of the samples. Fennel and coriander seed powder were used at concentrations of 2, 4 and 6% of wheat flour (w/w). The microbiological analysis was performed by total plate count, yeast and mould count, and Bacillus cereus count after 9, 12 and 15 weeks of storage. Sensory evaluation was conducted using hedonic scales at the end of storage durations. Results showed that spice infusion in frozen paratha significantly delayed the growth of aerobic mesophilic bacteria, yeasts and moulds, and Bacillus cereus during storage. The lowest log count was demonstrated by coriander at 6% in total plate count (3.85, 3.90 and 3.91 log10 CFU/g), and yeast and mould count (2.54, 2.59 and 2.60 log10 CFU/g) after 9, 12 and 15 weeks, respectively. Bacillus cereus was not detected throughout the storage durations. Fennel exhibited minimum activity against Bacillus cereus with no significant difference on log count reduction when compared with control. Coriander showed the highest decrease in both total plate count and Bacillus cereus count during the storage duration. Sensory evaluation result indicated that control sample exhibited the highest preference over all attributes when compared with fennel and coriander. Coriander-infused paratha was slightly darker in colour due to high concentration of 6%. Fennel yielded the lowest score in terms of taste among all samples. Fennel and coriander showed no significant difference for sensory acceptability. Overall, all frozen parathas were in good quality after 15 weeks of frozen storage. It can thus be concluded that fennel and coriander can be used as potential natural preservatives to inhibit the growth of microorganisms in paratha during frozen storage. Nevertheless, the optimum spice concentration should be determined to minimise the effects on the sensory attributes

Women's views and postpartum follow-up in the CHIPS Trial (Control of Hypertension in Pregnancy Study).

OBJECTIVE: To compare women's views about blood pressure (BP) control in CHIPS (Control of Hypertension In Pregnancy Study) (NCT01192412). DESIGN: Quantitative and qualitative analysis of questionnaire responses. SETTING: International randomised trial (94 sites, 15 countries). POPULATION/SAMPLE: 911 (92.9%) women randomised to 'tight' (target diastolic blood pressure, 85mmHg) or 'less tight' (target diastolic blood pressure, 100mmHg) who completed questionnaires. METHODS: A questionnaire was administered at ∼6-12 weeks postpartum regarding post-discharge morbidity and views about trial participation. Questionnaires were administered by the site co-ordinator, and contact was made by phone, home or clinic visit; rarely, data was collected from medical records. Quantitative analyses were Chi-square or Fisher's exact test for categorical variables, mixed effects multinomial logistic regression to adjust for confounders, and p<0.001 for statistical significance. NVivo software was used for thematic analysis of women's views. MAIN OUTCOME MEASURES: Satisfaction, measured as willingness to have the same treatment in another pregnancy or recommend that treatment to a friend. RESULTS: Among the 533 women in 'tight' (N=265) vs. 'less tight' (N=268) control who provided comments for qualitative analysis, women in 'tight' (vs. 'less tight') control made fewer positive comments about the amount of medication taken (5 vs. 28 women, respectively) and intensity of BP monitoring (7 vs. 17, respectively). However, this did not translate into less willingness to either have the same treatment in another pregnancy (434, 95.8% vs. 423, 92.4%, respectively; p=0.14) or recommend that treatment to a friend (435, 96.0% and 428, 93.4%, respectively; p=0.17). Importantly, although satisfaction remained high among women with an adverse outcome, those in 'tight' control who suffered an adverse outcome (vs. those who did not) were not consistently less satisfied, whereas this was not the case among women in 'less tight' control among whom satisfaction was consistently lower for the CHIPS primary outcome (p<0.001), severe hypertension (p≤0.01), and pre-eclampsia (p<0.001). CONCLUSIONS: Women in 'tight' (vs. 'less tight') control were equally satisfied with their care, and more so in the face of adverse perinatal or maternal outcomes

Third national surveillance of risk factors of non-communicable diseases (SuRFNCD-2007) in Iran: methods and results on prevalence of diabetes, hypertension, obesity, central obesity, and dyslipidemia

<p>Abstract</p> <p>Background</p> <p>The burden of non-communicable diseases is rising globally. This trend seems to be faster in developing countries of the Middle East. In this study, we presented the latest prevalence rates of a number of important non-communicable diseases and their risk factors in the Iranian population.</p> <p>Methods</p> <p>The results of this study are extracted from the third national Surveillance of Risk Factors of Non-Communicable Diseases (SuRFNCD-2007), conducted in 2007. A total of 5,287 Iranian citizens, aged 15–64 years, were included in this survey. Interviewer-administered questionnaires were applied to collect the data of participants including the demographics, diet, physical activity, smoking, history of hypertension, and history of diabetes. Anthropometric characteristics were measured and serum biochemistry profiles were determined on venous blood samples. Diabetes (fasting plasma glucose ≥ 126 mg/dl), hypertension (systolic blood pressure ≥ 140 mmHg, diastolic blood pressure ≥ 90 mmHg, or use of anti-hypertensive drugs), dyslipidemia (hypertriglyceridemia: triglycerides ≥ 150 mg/dl, hypercholesterolemia: total cholesterol ≥ 200 mg/dl), obesity (body mass index ≥ 30 kg/m²), and central obesity (waist circumference ≥ 80 cm in females and ≥ 94 cm in males) were identified and the national prevalence rates were estimated.</p> <p>Results</p> <p>The prevalence of diabetes, hypertension, obesity, and central obesity was 8.7% (95%CI = 7.4–10.2%), 26.6% (95%CI = 24.4–28.9%), 22.3% (95%CI = 20.2–24.5%), and 53.6% (95%CI = 50.4–56.8%), respectively. The prevalence of hypertriglyceridemia and hypercholesterolemia was 36.4% (95%CI = 34.1–38.9%) and 42.9% (95%CI = 40.4–45.4%), respectively. All of the mentioned prevalence rates were higher among females (except hypertriglyceridemia) and urban residents.</p> <p>Conclusion</p> <p>We documented a strikingly high prevalence of a number of chronic non-communicable diseases and their risk factors among Iranian adults. Urgent preventive interventions should be implemented to combat the growing public health problems in Iran.</p

Opportunities and threats to mandatory law of child restraint usage in Iran

Background and Aim: About one-third of Iranian children mortality is caused by injuries from which 36% occur due to road traffic injuries. Using child restraint embedded in vehicles can reduce road traffic fatalities by 71% for neonates and 54% for children. Based on its effectiveness in reduction of fatality and prevention of injury severity, child restraint usage mandatory law is a priority. Therefore, this study was conducted to assess opportunities and threats to mandatory law of child restraint usage in Iran.  Materials and Methods: Initially, a mixed methods research is carried out by a phenomenological qualitative study, a discussion session by traffic injuries’ stakeholders was performed to assess &amp; discuss the opportunities and threats to mandatory law of child restraint usage in Iran, by brain storming method to find the themes in the related topic. A structured questionnaire is later prepared and completed by the stakeholders in the area of road traffic injuries. Assigned scores of 0-100 were considered for each response and analysis of results was performed according to target themes &amp; the total score of the filled questionnaires.Results: Overall, 28 stakeholders participated in the study. According to the stakeholders, traffic police department obtained the highest score of 90 (from 0-100) as an organization to establish the mandatory law of child restraint usage, and acquired the score of 100 for future enforcement and monitoring. As threats and obstacle to the mandatory law of child restraint usage, lack of television and media campaigns and child restraint law and legislation, obtained the highest scores of 85 &amp; 70 respectively. And family sensitivity to their children's health, officials’ support and national facilities for broadcasting, and community awareness to use child restraints had the highest scores among existing opportunities and facilities in the country, by scores of 83, 69 and 68 respectively.Conclusion: Due to sensitivity of the family about their children’s health &amp; safety, and officials’ support to safety establishment through media campaigns, implementation and applicability of child restraint usage laws and legislations, and subsequent enforcement and monitoring seem practical.  ReferencesIsna.ir/fa, 13th May 2012.National Center for Statistics and Analysis. 2003, www.nhtsa.dot.gov.Global status report on road safety: time for action. Geneva, World Health Organization, 2009. (www.who.int/violence_injury_prevention/road_safety_status  date of access 12 September 2012.Jacobs G, Aaron­Thomas A, Astrop A. Estimating global road fatalities. London: Transport Research Laboratory, (TRL Report 445), First Published 2000, ISSN 0968-4107. Nantulya VM, Reich MR. The neglected epidemic: road traffic injuries in developing countries. BMJ 2002; 324:1139.Ameratunga S, Hijar M, Norton R. Road traffic injuries: confronting disparities to address a global health problem. Lancet 2004; 367:1533-1540.Kopits E, Cropper M. Traffic fatalities and economic growth. Policy Research Working Paper No. 3035. Washington, DC: World Bank; 2003. Available at: http://www.ntl.bts.gov/Lib/24000/24400/24490/25935_wps3 035.pdf. Data of access June 2012.Montazeri A. Road-traffic-related mortality in Iran: A descriptive study. Public Health 2004; 118: 110- 3.Soori H, Masoudinegad M R. Azari R M. Analysis of opportunities and legal obstacle in control of road traffic injury in Iran. Final report. Safety Promotion and Injury Prevention research center of Shahid Beheshti University of Medical Sciences, 2008.Kahane, C. An Evaluation of Child Passenger Safety: The Effectiveness and Benefits of Safety Seats, Washington, DC: National Highway Traffic Safety Administration, U.S. Department of Transportation1986.World report on child injury prevention, World Health Organization 2008. Global Status Report on Road Safety. www.who.int/entity/violence safety./state of road_safety_en.pdf , access28 August.Phyllis F. AGRAN, PAuL F. WEHRL E. Injury Reduction by Mandatory Child Passenger Safety Laws. AJPH 1985; 75(2): 129.Najafi H. Research methodology in educational science and psychology, Tehran, Ahsan, 2007, first edition, page 62.Zaza S, Sleet D A, Thompson R S, Sosin DM , Bolen J C. Reviews of evidence regarding interventions to increase use of child safety seats. Am J Prev Med 2001; 21(4 Suppl), 31-47.Desapriya E B, Iwase N, Pike I, Brussoni M, Papsdorf M. Child motor vehicle occupant and pedestrian casualties before and after enactment of child restraint seats legislation in Japan. Inj Control Saf Promot 2004; 11(4): 225-230.Staunton C, Davidson S, Kegler S, DawsonL, Powell K, Dellinger A. Critical gaps in child passenger safety practices, surveillance, and legislation: Georgia, 2001. Pediatrics 2005; 115(2): 372-379.Cameron L, Segedin E, Nuthall G, Thompson J. Safe restraint of the child passenger. J  Paediatr Child Health 2006; 42(12): 752-757.Bingham CR, Eby DW, Hockanson HM, Greenspan AI. Factors influencing the use of booster seats: a state-wide survey of parents. Accid Anal Prev. 2006; 38(5):1028-1037.Ehiri J, King W, Ejere H, Mouzon P. Effects of Interventions to Increase Use of Booster Seats in Motor Vehicles for 4-8 Year Olds. Washington, DC: AAA Foundation for Traffic Safety, 2006.GunnVL, Phillippi R M, Cooper WO. Improvement in Booster Seat Use in Tennessee. Pediatrics 2007; 119: 131-136.Winston FK, Kallan MJ, Elliott M R, Xie D, Durbin D R. Effect of Booster Seat Laws on Appropriate Restraint Use by Children 4 to 7 Years Old Involved in Crashes. Archives of Pediatric Adolescent Medicine 2007; 161:270-275.NHTSA. Preliminary Data Indicate That Booster Seat Laws Increase Child Safety Seat Use.Traffic Safety Facts. Traffic Tech, 331. Washington, DC: National Highway Traffic Safety Administration, 2007.Robertson L. Automobile seat belt use in selected countries, states and provinces with and without laws requiring belt use. Accid Anal and Prev 1978; 10:5-10.Stulginskas J V, Pless B. Effects of a seat belt law on child restraint use. Am J Dis Child 1983; 137:582-585. Wagenaar A C, Webster D W.  Preventing Injuries to Children Through Compulsory Automobile Safety Seat Use, Pediatrics 1986; 78 (4) : 662-672.Decina LE, Lococo KH, Ashburn W, William B, Rose H J. Identifying Strategies to Improve the Effectiveness of Booster Seat Laws, Draft Final Report, May 2008, www.nhtsa.dot.gov.Connell P M M. An evaluation of the Virginia 2002, Child passenger safety law: determining if the law reduced motor vehicle crash injuries and fatality. Virginia Common wealth University Richmond April, 2009.Seat-belts and child restraints: a road safety manual for decision-makers and practitioners London, FIA Foundation for the Automobile and Society, 2009.Istre G R, Stowe M, McCoy M A, Moore B, Culica D, Womack K N, Anderson R J. Anna B. Preventing unintentional injuries in Indigenous children and youth in Canada .Paediatr Child Health 2012; 17(7):393.