Muscular and tendon degeneration after achilles rupture: new insights into future repair strategies

Achilles tendon rupture is a frequent injury with an increasing incidence. After clinical surgical repair, aimed at suturing the tendon stumps back into their original position, the repaired Achilles tendon is often plastically deformed and mechanically less strong than the pre-injured tissue, with muscle fatty degeneration contributing to function loss. Despite clinical outcomes, pre-clinical research has mainly focused on tendon structural repair, with a lack of knowledge regarding injury progression from tendon to muscle and its consequences on muscle degenerative/regenerative processes and function. Here, we characterize the morphological changes in the tendon, the myotendinous junction and muscle belly in a mouse model of Achilles tendon complete rupture, finding cellular and fatty infiltration, fibrotic tissue accumulation, muscle stem cell decline and collagen fiber disorganization. We use novel imaging technologies to accurately relate structural alterations in tendon fibers to pathological changes, which further explain the loss of muscle mechanical function after tendon rupture. The treatment of tendon injuries remains a challenge for orthopedics. Thus, the main goal of this study is to bridge the gap between clinicians'' knowledge and research to address the underlying pathophysiology of ruptured Achilles tendon and its consequences in the gastrocnemius. Such studies are necessary if current practices in regenerative medicine for Achilles tendon ruptures are to be improved

The Cell Tracking Challenge: 10 years of objective benchmarking

The Cell Tracking Challenge is an ongoing benchmarking initiative that has become a reference in cell segmentation and tracking algorithm development. Here, we present a signifcant number of improvements introduced in the challenge since our 2017 report. These include the creation of a new segmentation-only benchmark, the enrichment of the dataset repository with new datasets that increase its diversity and complexity, and the creation of a silver standard reference corpus based on the most competitive results, which will be of particular interest for data-hungry deep learning-based strategies. Furthermore, we present the up-to-date cell segmentation and tracking leaderboards, an in-depth analysis of the relationship between the performance of the state-of-the-art methods and the properties of the datasets and annotations, and two novel, insightful studies about the generalizability and the reusability of top-performing methods. These studies provide critical practical conclusions for both developers and users of traditional and machine learning-based cell segmentation and tracking algorithms.Web of Science2071020101

Comunicación intraprofesional durante el cambio de turno a pie de cama. Percepciones del paciente

Introduction. More than 65% of the sentinel errors that take place in hospitals are related to communication issues. Among the safety measures promoted by OMS in order to reduce errors, there is the development of strategies that improve communication at shift change, such as including patients and relatives as an active part of the process. Nowadays, bedside communication at shift change (BCSC) is considered to be the Gold Standard for the transfer of responsibility, and it has been described that it improves the safety of the process and increases patient’s satisfaction. The aim of this research study is to explore patients‘ perceptions regarding BCSC. Methodology. Descriptive study carried out at the special hospitalization area of a tertiary care university hospital. Patients‘ perception was evaluated through a questionnaire developed after a literature review. Results. Three main issues arise from the analysis of the open-ended questions of the questionnaire: Patient’s Feelings and Robustness and Limitations of the process Discussion and conclusions. Patients perceive BCSC as being positive. They also report that conducting the process at the bedside may increase their safety, since it improves patient-centered care and the quality of the information transmitted and facilitates communication. However, the use of professional jargon may hinder the participation in the process.Introducción. Más de un 65% de los errores centinela que ocurren en los hospitales, están relacionados con problemas de comunicación. Entre las medidas de seguridad que promueve la OMS para reducir errores, se encuentra el desarrollo de estrategias que mejoren la comunicación en el cambio de turno, como el incluir a pacientes y familiares como parte activa del proceso. Hoy en día, la comunicación en el cambio de turno a pie de cama (CCTPC) se considera el Gold Standar para la transferencia de responsabilidad y se ha descrito que mejora la seguridad del proceso y aumenta la satisfacción del paciente. El objetivo de este estudio de investigación es explorar la percepción de los pacientes en relación a la CCTPC. Metodología. Estudio descriptivo llevado a cabo en el área de hospitalización especial de un hospital universitario de nivel terciario. Se evaluó la percepción de los pacientes a través de un cuestionario elaborado tras una revisión de la literatura. Resultados. Del análisis de las preguntas abiertas del cuestionario surgen 3 temas principales: Sentimientos del paciente, Fortalezas y Limitaciones del proceso. Discusión y conclusiones. Los pacientes perciben que la CCTPC es positiva, además refieren que llevar a cabo el proceso a pie de cama puede aumentar su seguridad, ya que se mejora la atención centrada en el paciente, la calidad de la información transmitida y se facilita la comunicación. Sin embargo, el uso de jerga profesional puede dificultar la participación en el proceso

On the Fertilizer Three Element Treatments of Rice Crops Sampled for Leaf Analysis by Field

Rice crops field experiment grown on fertilizer three element treatments was carried out in 1952-54. at Fukigami, Takigun, Hyogo Pref. The effect of N element was most remarkable from 1st year in total yield, and that of P was not significant until 3rd year. K effect was not significant in 1st year, but gradually became significant since 2nd year. Details of growth and yield results are shown in the tables