A giant thin stellar stream in the Coma Galaxy Cluster

The study of dynamically cold stellar streams reveals information about the gravitational potential where they reside and provides important constraints on the properties of dark matter. However, the intrinsic faintness of these streams makes their detection beyond Local environments highly challenging. Here, we report the detection of an extremely faint stellar stream (μg, max = 29.5 mag arcsec-2) with an extraordinarily coherent and thin morphology in the Coma Galaxy Cluster. This Giant Coma Stream spans ∼510 kpc in length and appears as a free-floating structure located at a projected distance of 0.8 Mpc from the center of Coma. We do not identify any potential galaxy remnant or core, and the stream structure appears featureless in our data. We interpret the Giant Coma Stream as being a recently accreted, tidally disrupting passive dwarf. Using the Illustris-TNG50 simulation, we identify a case with similar characteristics, showing that, although rare, these types of streams are predicted to exist in N-CDM. Our work unveils the presence of free-floating, extremely faint and thin stellar streams in galaxy clusters, widening the environmental context in which these objects are found ahead of their promising future application in the study of the properties of dark matter.</p

A giant thin stellar stream in the Coma Galaxy Cluster

The study of dynamically cold stellar streams reveals information about the gravitational potential where they reside and provides important constraints on the properties of dark matter. However, the intrinsic faintness of these streams makes their detection beyond Local environments highly challenging. Here, we report the detection of an extremely faint stellar stream (μg, max = 29.5 mag arcsec-2) with an extraordinarily coherent and thin morphology in the Coma Galaxy Cluster. This Giant Coma Stream spans ∼510 kpc in length and appears as a free-floating structure located at a projected distance of 0.8 Mpc from the center of Coma. We do not identify any potential galaxy remnant or core, and the stream structure appears featureless in our data. We interpret the Giant Coma Stream as being a recently accreted, tidally disrupting passive dwarf. Using the Illustris-TNG50 simulation, we identify a case with similar characteristics, showing that, although rare, these types of streams are predicted to exist in N-CDM. Our work unveils the presence of free-floating, extremely faint and thin stellar streams in galaxy clusters, widening the environmental context in which these objects are found ahead of their promising future application in the study of the properties of dark matter.</p

A giant thin stellar stream in the Coma Galaxy Cluster

The study of dynamically cold stellar streams reveals information about the gravitational potential where they reside and provides important constraints on the properties of dark matter. However, the intrinsic faintness of these streams makes their detection beyond Local environments highly challenging. Here, we report the detection of an extremely faint stellar stream (μg, max = 29.5 mag arcsec-2) with an extraordinarily coherent and thin morphology in the Coma Galaxy Cluster. This Giant Coma Stream spans ∼510 kpc in length and appears as a free-floating structure located at a projected distance of 0.8 Mpc from the center of Coma. We do not identify any potential galaxy remnant or core, and the stream structure appears featureless in our data. We interpret the Giant Coma Stream as being a recently accreted, tidally disrupting passive dwarf. Using the Illustris-TNG50 simulation, we identify a case with similar characteristics, showing that, although rare, these types of streams are predicted to exist in N-CDM. Our work unveils the presence of free-floating, extremely faint and thin stellar streams in galaxy clusters, widening the environmental context in which these objects are found ahead of their promising future application in the study of the properties of dark matter.</p

A giant thin stellar stream in the Coma Galaxy Cluster

The study of dynamically cold stellar streams reveals information about the gravitational potential where they reside and provides important constraints on the properties of dark matter. However, the intrinsic faintness of these streams makes their detection beyond Local environments highly challenging. Here, we report the detection of an extremely faint stellar stream (μg, max = 29.5 mag arcsec-2) with an extraordinarily coherent and thin morphology in the Coma Galaxy Cluster. This Giant Coma Stream spans ∼510 kpc in length and appears as a free-floating structure located at a projected distance of 0.8 Mpc from the center of Coma. We do not identify any potential galaxy remnant or core, and the stream structure appears featureless in our data. We interpret the Giant Coma Stream as being a recently accreted, tidally disrupting passive dwarf. Using the Illustris-TNG50 simulation, we identify a case with similar characteristics, showing that, although rare, these types of streams are predicted to exist in N-CDM. Our work unveils the presence of free-floating, extremely faint and thin stellar streams in galaxy clusters, widening the environmental context in which these objects are found ahead of their promising future application in the study of the properties of dark matter.</p

A giant thin stellar stream in the Coma Galaxy Cluster

The study of dynamically cold stellar streams reveals information about the gravitational potential where they reside and provides important constraints on the properties of dark matter. However, the intrinsic faintness of these streams makes their detection beyond Local environments highly challenging. Here, we report the detection of an extremely faint stellar stream (μg, max = 29.5 mag arcsec-2) with an extraordinarily coherent and thin morphology in the Coma Galaxy Cluster. This Giant Coma Stream spans ∼510 kpc in length and appears as a free-floating structure located at a projected distance of 0.8 Mpc from the center of Coma. We do not identify any potential galaxy remnant or core, and the stream structure appears featureless in our data. We interpret the Giant Coma Stream as being a recently accreted, tidally disrupting passive dwarf. Using the Illustris-TNG50 simulation, we identify a case with similar characteristics, showing that, although rare, these types of streams are predicted to exist in N-CDM. Our work unveils the presence of free-floating, extremely faint and thin stellar streams in galaxy clusters, widening the environmental context in which these objects are found ahead of their promising future application in the study of the properties of dark matter.</p

Transglutaminase 6: a protein associated with central nervous system development and motor function.

Transglutaminases (TG) form a family of enzymes that catalyse various post-translational modifications of glutamine residues in proteins and peptides including intra- and intermolecular isopeptide bond formation, esterification and deamidation. We have characterized a novel member of the mammalian TG family, TG6, which is expressed in a human carcinoma cell line with neuronal characteristics and in mouse brain. Besides full-length protein, alternative splicing results in a short variant lacking the second β-barrel domain in man and a variant with truncated β-sandwich domain in mouse. Biochemical data show that TG6 is allosterically regulated by Ca(2+) and guanine nucleotides. Molecular modelling indicates that TG6 could have Ca(2+) and GDP-binding sites related to those of TG3 and TG2, respectively. Localization of mRNA and protein in the mouse identified abundant expression of TG6 in the central nervous system. Analysis of its temporal and spatial pattern of induction in mouse development indicates an association with neurogenesis. Neuronal expression of TG6 was confirmed by double-labelling of mouse forebrain cells with cell type-specific markers. Induction of differentiation in mouse Neuro 2a cells with NGF or dibutyryl cAMP is associated with an upregulation of TG6 expression. Familial ataxia has recently been linked to mutations in the TGM6 gene. Autoantibodies to TG6 were identified in immune-mediated ataxia in patients with gluten sensitivity. These findings suggest a critical role for TG6 in cortical and cerebellar neurons

Transglutaminase 2 Undergoes a Large Conformational Change upon Activation

Human transglutaminase 2 (TG2), a member of a large family of enzymes that catalyze protein crosslinking, plays an important role in the extracellular matrix biology of many tissues and is implicated in the gluten-induced pathogenesis of celiac sprue. Although vertebrate transglutaminases have been studied extensively, thus far all structurally characterized members of this family have been crystallized in conformations with inaccessible active sites. We have trapped human TG2 in complex with an inhibitor that mimics inflammatory gluten peptide substrates and have solved, at 2-Å resolution, its x-ray crystal structure. The inhibitor stabilizes TG2 in an extended conformation that is dramatically different from earlier transglutaminase structures. The active site is exposed, revealing that catalysis takes place in a tunnel, bridged by two tryptophan residues that separate acyl-donor from acyl-acceptor and stabilize the tetrahedral reaction intermediates. Site-directed mutagenesis was used to investigate the acyl-acceptor side of the tunnel, yielding mutants with a marked increase in preference for hydrolysis over transamidation. By providing the ability to visualize this activated conformer, our results create a foundation for understanding the catalytic as well as the non-catalytic roles of TG2 in biology, and for dissecting the process by which the autoantibody response to TG2 is induced in celiac sprue patients

Interplay between transglutaminases and heparan sulphate in progressive renal scarring

Transglutaminase-2 (TG2) is a new anti-fibrotic target for chronic kidney disease, for its role in altering the extracellular homeostatic balance leading to excessive build-up of matrix in kidney. However, there is no confirmation that TG2 is the only transglutaminase involved, neither there are strategies to control its action specifically over that of the conserved family-members. In this study, we have profiled transglutaminase isozymes in the rat subtotal nephrectomy (SNx) model of progressive renal scarring. All transglutaminases increased post-SNx peaking at loss of renal function but TG2 was the predominant enzyme. Upon SNx, extracellular TG2 deposited in the tubulointerstitium and peri-glomerulus via binding to heparan sulphate (HS) chains of proteoglycans and co-associated with syndecan-4. Extracellular TG2 was sufficient to activate transforming growth factor-β1 in tubular epithelial cells, and this process occurred in a HS-dependent way, in keeping with TG2-affinity for HS. Analysis of heparin binding of the main transglutaminases revealed that although the interaction between TG1 and HS is strong, the conformational heparin binding site of TG2 is not conserved, suggesting that TG2 has a unique interaction with HS within the family. Our data provides a rationale for a novel anti-fibrotic strategy specifically targeting the conformation-dependent TG2-epitope interacting with HS

Epidermal Transglutaminase (TGase 3) Is Required for Proper Hair Development, but Not the Formation of the Epidermal Barrier

Transglutaminases (TGase), a family of cross-linking enzymes present in most cell types, are important in events as diverse as cell-signaling and matrix stabilization. Transglutaminase 1 is crucial in developing the epidermal barrier, however the skin also contains other family members, in particular TGase 3. This isoform is highly expressed in the cornified layer, where it is believed to stabilize the epidermis and its reduction is implicated in psoriasis. To understand the importance of TGase 3 in vivo we have generated and analyzed mice lacking this protein. Surprisingly, these animals display no obvious defect in skin development, no overt changes in barrier function or ability to heal wounds. In contrast, hair lacking TGase 3 is thinner, has major alterations in the cuticle cells and hair protein cross-linking is markedly decreased. Apparently, while TGase 3 is of unique functional importance in hair, in the epidermis loss of TGase 3 can be compensated for by other family members

Transglutaminase activation in neurodegenerative diseases

The following review examines the role of calcium in promoting the in vitro and in vivo activation of transglutaminases in neurodegenerative disorders. Diseases such as Alzheimer's disease, Parkinson's disease and Huntington's disease exhibit increased transglutaminase activity and rises in intracellular calcium concentrations, which may be related. The aberrant activation of transglutaminase by calcium is thought to give rise to a variety of pathological moieties in these diseases, and the inhibition has been shown to have therapeutic benefit in animal and cellular models of neurodegeneration. Given the potential clinical relevance of transglutaminase inhibitors, we have also reviewed the recent development of such compounds