The Role and Significance of Taṣawwuf in Modern-Day Crisis

The present manuscript aims to assess the important features of Taṣawwuf and its position in the contemporary world. Moreover, this study will help to understand the paramount role of Taṣawwuf in pulling out humanity from these crises and its importance in espousing the society in terms of socio-religious and spiritual development. The suggested methodology for this manuscript will be exploratory, descriptive, and historical to explore the contemporary importance of Taṣawwuf and its main characteristics. This study reveals the continuing significance of Taṣawwuf in modern times, where, religious extremism, violence, human value crises, social disharmony, and an imbalance between spirituality and mundane concerns of humanity are some issues that are confronting human beings in the contemporary world. Hence this study found that the legitimacy of Taṣawwuf in modern society still exists.Contribution: This article makes a new discovery regarding the continued existence of Taṣawwuf and its role in maintaining peace, harmony, and religious tolerance in contemporary society and also reveals the role of Taṣawwuf in the spiritual development of human beings and maintaining a balance between the spirituality and mundane ends

The Role and Significance of Taṣawwuf in Modern-Day Crisis

The present manuscript aims to assess the important features of Taṣawwuf and its position in the contemporary world. Moreover, this study will help to understand the paramount role of Taṣawwuf in pulling out humanity from these crises and its importance in espousing the society in terms of socio-religious and spiritual development. The suggested methodology for this manuscript will be exploratory, descriptive, and historical to explore the contemporary importance of Taṣawwuf and its main characteristics. This study reveals the continuing significance of Taṣawwuf in modern times, where, religious extremism, violence, human value crises, social disharmony, and an imbalance between spirituality and mundane concerns of humanity are some issues that are confronting human beings in the contemporary world. Hence this study found that the legitimacy of Taṣawwuf in modern society still exists.Contribution: This article makes a new discovery regarding the continued existence of Taṣawwuf and its role in maintaining peace, harmony, and religious tolerance in contemporary society and also reveals the role of Taṣawwuf in the spiritual development of human beings and maintaining a balance between the spirituality and mundane ends

Determinants of academic stress and stress-related selfmedication practice among undergraduate male pharmacy and medical students of a tertiary educational institution in Saudi Arabia

Purpose: To identify factors that promote academic stress and stress-related self-medication practice among undergraduate male students of pharmacy and medical colleges at Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia. Methods: A cross-sectional survey was conducted among undergraduate students of pharmacy and medical colleges of the university. The study used Perceived Stress Scale (PSS) to document academic stress. The responses of the students were analyzed using SPSS version 22. Results: As many as 51.6 % of students’ perceived moderate stress. The majority of students (55.9 %) highlighted examination as a stressor followed by course load (43.2 %) and cGPA (40.4 %). Prevalence of self-medication was 31.58 and 29.20 % among pharmacy and medical students, respectively. Most of the students consumed caffeine (63.8 %) and nicotine (17.8 %) as a drug. Students blamed heavy course load (23.9 %), followed by assignment load (23 %) and examination (21.1 %) for indulging in self-medication. Conclusion: Academic stress in undergraduate students in health disciplines is perceived to be high by the students. Examinations, course load and lack of time for leisure are major determinants of stress. Caffeine and nicotine are most frequently used by a majority of the students for self-medication. Keywords: Stress, Self-medication, Stressor, Caffeine, Nicotine, Student

Kinetics & Mechanism of Base-catalysed Hydrolysis of Formamide-Effect of Alkalinity on the Reactive Tetrahedral Addition Intermediates

961-96

Quantification of rutin in rats brain by UHPLC/ESI-Q-TOF-MS/MS after intranasal administration of rutin loaded chitosan nanoparticles

Rutin (RT), an antioxidant drug, has been utilized to treat cerebral ischemia hence a sensitive quantification method for estimation of RT in brain homogenate is necessary to develop. This study aims to prepare RT loaded Chitosan Nanoparticles (RT-CS-NPs) develop and validate ultra-high performance liquid chromatography-electrospray ionization-synapt mass spectrometric method Synapt Mass Spectrometry (Synapt MS) (UHPLC/ESI-QTOF-MS/MS) for quantification of RT in brain homogenate from Wistar rat. The process of chromatographic separation was carried out on Waters ACQUITY UPLC™ with the components of separation in detail as; column: BEH C-18 with dimension as 2.1 mm×100 mm and particle size 1.7 µm, mobile phase: acetonitrile (85 % v/v/v): 2 mM ammonium formate (15 % v/v/v): formic acid (0.1 % v/v/v) and flow rate: 0.25 mL/min. Liquid-liquid extraction method (LLE), in mixture, i.e. ethyl acetate:acetonitrile, was considered to optimize the recovery of analyte from the brain homogenate of Wistar rat. Over a total run time of 5 minutes, the elution time for RT and internal standard (IS), i.e. Tolbutamide, observed was 2.67 and 2.82 min respectively whereas the transition observed for RT and IS was at m/z 611.1023/303.1071 and 271.1263/155.1073, respectively. Results, regarding various processes and parameters studied for RT as summarized, established a linear dynamic range over a concentration range of 1.00 ng/mL - 1000.0 ng/mL with r2; 0.9991±0.0010. Accuracy for intra and inter-assay in terms of % CV revealed a range of 0.45- 2.11 whereas lower limit of detection (LOD) and quantitation (LOQ) observed was 0.09 ng/mL and 0.142 ng/mL, respectively. The analyte stability as well as method specificity and accuracy, i.e. recovery > 86 %, supports the idea for application of current developed method in order to quantify and evaluate the RT-loaded-CS-NPs for RT determination in brain homogenate after intranasal drug delivery

Modifiable and non-modifiable risk factors in ischemic stroke: a meta-analysis

Background: Stroke is a heterogeneous, multifactorial disease regulated by modifiable and non-modifiable risk factors like total cholesterol, triglycerides, high density lipid-cholesterol (HDL-C), low density lipid-cholesterol (LDL-C) and glucose and non-modifiable factors like age. Evaluation of these risk factors for ischemic stroke may contribute to more effective prevention. Methods: This meta-analysis investigated the data up to 2018 with respect to age, total cholesterol, triglycerides, high density lipid-cholesterol (HDL-C), low density lipid-cholesterol (LDL-C) and glucose levels in the Ischemic stroke patients and controls. Data from each eligible study was extracted such as year of publication, first author name, ethnicity/country, number of ischemic stroke patients and healthy controls and studies were subjected to heterogeneity analysis. Results: Meta-analysis of the modifiable and non-modifiable risk factors like total cholesterol, HDL-C and LDL-C glucose, triglycerides levels and age were not having significantly associated with ischemic stroke (p>0.05). However meta-analysis of triglyceride yielded significant association ischemic stroke with overall 95% CI (-0.62- (-0.09) and P= 0.007 and glucose also showed significant association with ischemic stroke with 95% CI (-1.08- (-0.14), P=0.01. Studies were subjected to heterogeneity which revealed significant heterogeneity with (P<0.00) and I2>96%. Conclusion: Meta-analysis revealed that levels of triglyceride and glucose are the predisposing factors for ischemic stroke in the Asian population. DOI: https://dx.doi.org/10.4314/ahs.v19i2.36 Cite as: Cui Q, Naikoo NA. Modifiable and non-modifiable risk factors in Ischemic stroke: meta-analysis. Afri Health Sci.2019;19(2): 2121-2129. https://dx.doi.org/10.4314/ahs.v19i2.3

HEPATOPROTECTIVE EFFECTS OF PUNICA GRANATUM FRUIT AGAINST D-GALACTOSAMINE INDUCED HEPATOTOXICITY IN RATS: IN VITRO AND IN VIVO STUDIES

Objective: Hepatoprotective activity of Punica granatum Linn. (Family: Punicaceae) was evaluated by in vitro and in vivo model. HepG2 cell lines were used for in vitro study and D-Galactosamine (D-GalN) induced hepatic damage model for in vivo evaluation.Methods: Hepatoprotective potential was assessed by measuring serum level of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase, total bilirubin, and albumin. Enzymatic antioxidant parameters like TBARs (thiobarbituric acid reactive substances), GSH (glutathione), SOD (superoxide dismutase) and CAT (catalase) also evaluated in this study. Results: Rats treated with D-GalN showed a significant increase in serum levels of SGOT (serum glutamate oxaloacetate transaminase), SGPT (serum glutamate pyruvate transaminase), ALP (alkaline phosphatase), bilirubin and TBARs, reflecting liver damage. The in vitro study indicates a beneficial effect of aqueous extract of P. granatum in comparison with methanolic extract, on D-GalN induced toxicity to HepG2 cells. On the basis of in vitro study, aqueous extract was selected for in vivo evaluation. The aqueous extract of P. granatum significantly reduced the elevated serum biomarkers, indicating the recovery of hepatocellular injury. It was also observed that D-GalN induced a significant decrease in GSH, SOD, CAT, protein, and albumin level were increased on the treatment of the rats with aqueous extract of P. granatum. The findings were also confirmed by histopathological studies. Periportal area and extensive hepatocyte damage and haemorrhage are seen in D-GalN treated group. The portal triad with less degree of inflammatory cell infiltration around the bile duct is seen in P. granatum (500 mg/kg)+(D-GalN) treated group.Conclusion: Results of this study revealed that P. granatum fruit could afford a significant protection for the alleviation of hepatic toxicity. Possible mechanism may involve its action against oxidative stress.Â

Big data and a smarter university: a literature review

Using big data in a university is ‘the thing’ today since almost all information today is digitalized. Big data ensures better data processing, analysis and sharing, thus increasing productivity and researchers would have a great time doing their researches. A smart university is a university which uses technologies which could increase the productivity of knowledge management. Changing into a smart university is definitely a smart move because it is important to be competitive in a world full of formidable competitors. Research has proven that smart universities such as Caltech University and Northwestern University which are among the top universities in the world has also endeavored into the big data arena

The SARS Coronavirus 3a Protein Causes Endoplasmic Reticulum Stress and Induces Ligand-Independent Downregulation of the Type 1 Interferon Receptor

The Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) is reported to cause apoptosis of infected cells and several of its proteins including the 3a accessory protein, are pro-apoptotic. Since the 3a protein localizes to the endoplasmic reticulum (ER)-Golgi compartment, its role in causing ER stress was investigated in transiently transfected cells. Cells expressing the 3a proteins showed ER stress based on activation of genes for the ER chaperones GRP78 and GRP94. Since ER stress can cause differential modulation of the unfolded protein response (UPR), which includes the inositol-requiring enzyme 1 (IRE-1), activating transcription factor 6 (ATF6) and PKR-like ER kinase (PERK) pathways, these were individually tested in 3a-expressing cells. Only the PERK pathway was found to be activated in 3a-expressing cells based on (1) increased phosphorylation of eukaryotic initiation factor 2 alpha (eIF2α) and inhibitory effects of a dominant-negative form of eIF2α on GRP78 promoter activity, (2) increased translation of activating transcription factor 4 (ATF4) mRNA, and (3) ATF4-dependent activation of the C/EBP homologous protein (CHOP) gene promoter. Activation of PERK affects innate immunity by suppression of type 1 interferon (IFN) signaling. The 3a protein was found to induce serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and to increase IFNAR1 ubiquitination. Confocal microscopic analysis showed increased translocation of IFNAR1 into the lysosomal compartment and flow cytometry showed reduced levels of IFNAR1 in 3a-expressing cells. These results provide further mechanistic details of the pro-apoptotic effects of the SARS-CoV 3a protein, and suggest a potential role for it in attenuating interferon responses and innate immunity

Differential Effects of Natural Grazing and Feedlot Feeding on Yak Fecal Microbiota

Variation in food and diet shapes the diversity of the gut microbiota of ruminants. The present study investigated the microbial diversity in the fecal microbiota of yaks reared under natural grazing and feedlot system. A total of 48 domestic yaks with an average age of 7.5 years were selected from two different grazing habitats: one group grazed on natural pasture (grazing yaks—GY) while the other group was fed fodder and concentrate (feedlot yaks—FY). Crude protein, non-fiber carbohydrate, hemicelluloses, and digestible dry matter contents of natural pastures were higher than those in the feedlot. The lower insoluble fiber contents were found in grazing land. The 16S rRNA gene sequencing revealed 675 and 348 unique operational taxonomic units (OTUs) in the GY and FY, respectively, in addition to 1,778 common OTUs. Overall, a total of 9,891 OTUs were identified as a whole, of which 6,160 OTUs were from GY and 3,731 were from FY. Shannon index analysis revealed a higher bacterial diversity in GY than FY. At the phylum level, Firmicutes were dominant bacterial taxa in both groups. The relative abundance of Firmicutes in GY (56% ± 0.05) was higher than in FY (41% ± 0.08). At the family level, GY had a significantly higher abundance of Ruminococcaceae (p < 0.001) and Rikenellaceae (p < 0.001) than FY, but FY had a significantly higher abundance of Prevotellaceae than GY (p < 0.001). At the genus level, abundances of Faecalibacterium, Alloprevotella, and Succinivibrio were higher in FY than in GY. This study presents novel information on fecal bacterial composition and diversity in yaks reared under two different production systems