Me destino

Treballs Finals de Grau de Belles Arts. Facultat de Belles Arts. Universitat de Barcelona, Curs: 2017, Tutor: Aramendía, Manuel[spa] El proyecto “Me destino” es una performance que trata el concepto del destino como trayecto para llegar a un fin. Este fin pensado como ‘lugar prometido’. Vacío. En esta acción se habla de adaptación, de lugar, de recorrido, de dirección y de promesa. Creer y dudar. El elemento principal son las sillas, éstas se presentan como lugares. Juega un papel muy importante la luz, que recorta espacios y crea caminos. Aparece la repetición como movimiento del “no ser” y el sonido de voz y percusión para crear ambientes.[eng] The project “Me destino” is a performance that deals with the concept of destiny as a journey to reach a final destination. This destination is thought of as the ‘promised place’. Void. This work talks about adaptation, place, path, direction and promise. To believe and to doubt. The main element are the chairs, presented as places. Light plays an important role, outlining spaces and creating pathways. Repetition appears as the movement of “not being”, and the sound of voice and percussion to build environment

Cambios en el espectro etiológico de la fiebre de duración intermedia en el sur de Europa

Falta palabras claveLa Fiebre de Duración Intermedia (FDI) es un motivo de consulta frecuente en Andalucía, de pronóstico generalmente favorable y cuya etiología no se diagnostica en una proporción importante de casos utilizando métodos convencionales. Objetivos: Describir las características epidemiológicas, etiológicas, clínicas y pronósticas de la FDI en nuestra Comunidad y evaluar la rentabilidad de otros métodos no convencionales para mejorar el diagnóstico etiológico. Métodos: Estudio prospectivo multicéntrico de pacientes adultos consecutivos con FFDI según criterios preestablecidos atendidos en nueve hospitales andaluces durante 3 años (01/11/2006-01/11/2009).Evaluación protocolizada que incluía anamnesis, exploración física, pruebas complementarias básicas, 2 determinaciones serológicas seriadas (Brucella melitensis, Coxiella burnetti, Rickettsias typhi y conorii, CMV y VEB), ARN-VIH, hemocultivos con fiebre y extracción de muestras sanguíneas para técnicas microbiológicas adicionales para casos sin diagnóstico o con diagnóstico dudoso, así como otras exploraciones individualizadas según criterios clínicos. Recogida estandarizada de datos y análisis descriptivo. Resultados: Se incluyeron 333 pacientes (mediana 35 años, rango 15-79); 66,7% varones, 57,2% de ámbito urbano), mayoritariamente (92%) procedentes de las urgencias hospitalarias. La media de duración de la fiebre antes de la primera evaluación fue de 15 días (7-24) y el 45% habían recibido antimicrobianos previos. Los hallazgos analíticos más frecuentes fueron elevación de transaminasas (GPT 61%, GOT 44%, GGT 64%), monocitosis (27%) y linfopenia (27%). En 214 pacientes (64,2%) se alcanzó un diagnóstico etiológico: 172 (52,7%) infecciones sistémicas (60 CMV; 48 fiebre Q; 28 VEB; 8 brucelosis; 8 tifus murinos: 6 primoinfecciones por VIH; 5 fiebres botonosas); 15 (4,6%) infecciones localizadas y 21 (6,7%) procesos no infecciosos. En los 119 restantes (35,7%) no se llegó a un diagnóstico etiológico. 50 pacientes (15%) requirieron ingreso hospitalario y 3 fallecieron. Las infecciones por CMV y VEB se asociaron significativamente con cifras de LDH >500 UI/L; VSG 4000/ul, mientras que valores de linfocitos < 1500/ul y PCR < 20 mg/dl fueron más frecuentes en el grupo de etiologías tratables con doxiciclina. En 208 pacientes se completó estudio centralizado adicional, permitiendo diagnosticar 25 de 106 (23,5%) sin diagnóstico previo. En total se obtuvieron mediante serologías adicionales 18 casos de Bartonella henselae, 3 casos de Anaplasmaspp., 3 Rickettsia felis, y 2rickettsiosis exantemática no filiada (SFGR). Entre el grupo de virus, 6 pacientes se diagnosticaron de virus herpes humano 6 (VHH6). El 81% de los diagnósticos de viriasis se confirmaron mediante estudios adicionales. Conclusiones: Las infecciones sistémicas continúan siendo la principal causa del FDI en nuestro medio, con un predominio de las infecciones víricas (CMV, VEB) y de la fiebre Q, un descenso de las otras zoonosis clásicas. Determinados parámetros analíticos permitirían diferenciar entre los grandes grupos etiológicos. La aplicación de técnicas microbiológicas no habituales podría reducir la proporción de casos que quedan sin diagnóstico etiológico tras un estudio convencional (35,8%)

Impact of transient down-regulation of DREAM in human embryonic stem cell pluripotency: The role of DREAM in the maintenance of hESCs

Little is knownabout the functions of downstreamregulatory element antagonist modulator (DREAM) inembryonic stem cells (ESCs). However, DREAM interacts with cAMP response element-binding protein (CREB) in a Ca2+-dependent manner, preventing CREB binding protein (CBP) recruitment. Furthermore, CREB and CBP are involved in maintaining ESC self-renewal and pluripotency. However, a previous knockout study revealed the protective function of DREAMdepletion in brain aging degeneration and that aging is accompanied by a progressive decline in stem cells (SCs) function. Interestingly, we found that DREAM is expressed in different cell types, including human ESCs (hESCs), human adipose-derived stromal cells (hASCs), human bone marrow-derived stromal cells (hBMSCs), and human newborn foreskin fibroblasts (hFFs), and that transitory inhibition of DREAMin hESCs reduces their pluripotency, increasing differentiation.We stipulate that these changes are partly mediated by increased CREB transcriptional activity. Overall, our data indicates that DREAMacts in the regulation of hESC pluripotency and could be a target to promote or prevent differentiation in embryonic cells.Junta de Andalucía, Consejería de Innovación Ciencia y Empresa, FEDER CTS-6505; INP-2011- 1615-900000; P10-CVI-6095Instituto de Salud Carlos III, FEDER RD12/0019/0028; PI10/00964; PI14/0101

Functional Estimation of the Random Rate of a Cox Process

The intensity of a doubly stochastic Poisson process (DSPP) is also a stochastic process whose integral is the mean process of the DSPP. From a set of sample paths of the Cox process we propose a numerical method, preserving the monotone character of the mean, to estimate the intensity on the basis of the functional PCA. A validation of the estimation method is presented by means of a simulation as well as a comparison with an alternative estimation method.Projects MTM2007-63793 of Dirección General de Investigación, Ministerio de Ciencia, P06-FQM-01470 from Consejería de Innovación, Ciencia y Empresa de la Junta de Andalucía and MTM2007-66791 of Plan Nacional I+D, Ministerio de Ciencia y Tecnología jointly by the FEDER and grant FQM-307 of Conserjería de Innovación de la Junta de Andalucía, all of them in Spai

miR-7 Modulates hESC Differentiation into Insulin-Producing Beta-like Cells and Contributes to Cell Maturation

Human pluripotent stem cells retain the extraordinary capacity to differentiate into pancreatic beta cells. For this particular lineage, more effort is still required to stress the importance of developing an efficient, reproducible, easy, and cost-effective differentiation protocol to obtain more mature, homogeneous, and functional insulin-secreting cells. In addition, microRNAs (miRNAs) have emerged as a class of small non-coding RNAs that regulate many cellular processes, including pancreatic differentiation. Some miRNAs are known to be preferentially expressed in islets. Of note, miR-375 and miR-7 are two of the most abundant pancreatic miRNAs, and they are necessary for proper pancreatic islet development. Here we provide new insight into specific miRNAs involved in pancreatic differentiation. We found that miR-7 is differentially expressed during the differentiation of human embryonic stem cells (hESCs) into a beta cell-like phenotype and that its modulation plays an important role in generating mature pancreatic beta cells. This strategy may be exploited to optimize the potential for in vitro differentiation of hESCs into insulin-producing beta-like cells for use in preclinical studies and future clinical applications as well as the prospective uses of miRNAs to improve this process.Spanish Ministry of Economy and Competitiveness BFU2016-74932-C2 BFU2013-45564-C2FEDER Funds PI-0272-2017Andalusian Regional Ministry of Health PI-0272-2017European Cooperation in Science and Technology BM1305Spanish Ministry of Economy, Industry and Competitiveness CD16/00118Spanish Institute of Health Carlos III PI16/00259 PI17/02104 RD16/0011/0034 CD16/0011

Notificaciones electrónicas tributarias: Estatuto jurídico y su problemática

En los últimos años, el régimen de las notificaciones administrativas ha experimentado un gran avance en nuestro país. Impulsado por la Unión Europea, el legislador español consagró en la Ley 39/2015 el principio de preferencia de medios electrónicos en la notificación, imponiendo en algunos casos la notificación electrónica para determinados grupos y procedimientos. Uno de los ámbitos más afectados por el avance de la administración electrónica es el ámbito tributario. Por ello, nuestro trabajo se centrará en estudiar la notificación electrónica en este ámbito concreto, la cual ha supuesto indudables ventajas, pero también ha provocado que se planteen una serie de problemas tanto para la administración pública como para el ciudadano a raíz de la nueva normativa.In recent years, the system of administrative notifications has made great strides in our country. Encouraged by the European Union, the Spanish legislator enshrined in Law 39/2015 the principle of preference of electronic means in notification, imposing in some cases electronic notification for certain groups and procedures. One of the areas most affected by the progress of e-Government is taxation. Therefore, our work will focus on studying electronic reporting in this specific area, which has been of undoubted benefit, but it has also caused a number of problems for both the public administration and the citizien as a result of the new regulations

Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder

From 106 human blastocyts donate for research after in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for monogenetic disorder, 3 human embryonic stem cells (hESCs) HVR1, HVR2 and HVR3 were successfully derived. HVR1 was assumed to be genetically normal, HVR2 carrying Becker muscular dystrophy and HVR3 Hemophilia B. Despite the translocation t(9;15)(q34.3;q14) detected in HVR2, all the 3 cell lines were characterised in vitro and in vivo as normal hESCs lines and were registered in the Spanish Stem Cell Bank.Junta de Andalucía FEDER TCMR0021/06, PI246-2008Instituto de Salud Carlos III (FEDER) RD12/0019/0028, RD012/0036/0017, PI10/00964, PI11/02923, PI14/0101

LA INFLUENCIA DE LA DIVERSIFICACIÓN Y EXPERIENCIA INTERNACIONAL EN LA ESTRATEGIA MEDIOAMBIENTAL PROACTIVA DE LAS EMPRESAS

RESUMENEste trabajo tiene como objetivo analizar la influencia que la internacionalización puede tener sobre la adopción de una estrategia medioambiental más proactiva por parte de las empresas. Para conseguir este objetivo realizamos 103 entrevistas personales a los directores generales de empresas exportadoras españolas pertenecientes al sector de la alimentación. Nuestros resultados muestran que un alto grado de diversificación internacional favorece el desarrollo de una estrategia medioambiental más proactiva. Además, la experiencia internacional de las empresas contribuye también a la adquisición de capacidades medioambientales valiosas. Por tanto, la internacionalización permite a las empresas desarrollar una estrategia más avanzada en gestión medioambiental. Finalmente, recogemos una serie de implicaciones de carácter práctico para directivos y reguladores públicos.ABSTRACTWe analyze the influence of the firms' internationalization process of firms on the adoption of a proactive environmental strategy. Using direct interviews with the CEOs of 103 export Spanish firms from the food industry, we show that a high degree of international diversification encourages these firms to take advantage of different environmental competitive advantages from the different regions where they operate. In addition, firms' international experience contributes to acquiring valuable environmental capabilities. Thus, our results support that internationalization leads firms to develop an advanced posture to environmental issues and adopt a proactive environmental strategy. Our paper discusses implications for managers and policy-makers

Modelling the mean of a doubly stochastic Poisson process by functional data analysis

A new procedure for estimating the mean process of a doubly stochastic Poisson process is introduced. The proposed estimation is based on monotone piecewise cubic interpolation of the sample paths of the mean. In order to estimate the continuous time structure of the mean process functional principal component analysis is applied to its trajectories previously adapted to their functional form. A validation of the estimation method is presented by means of some simulations.Projects MTM2004-05992 of Dirección General de Investigación, and MTM2004-04230 of Plan Nacional de I+D+I, Ministerio de Ciencia y Tecnología jointly by the FEDE

Distribution and seasonal variability in the benthic eukaryotic community of Río Tinto (SW, Spain), an acidic, high metal extreme environment

Author Posting. © The Author(s), 2007. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Systematic and Applied Microbiology 30 (2007): 531-546, doi:10.1016/j.syapm.2007.05.003.The eukaryotic community of Río Tinto (SW, Spain) was surveyed fall, winter, and spring through the combined use of traditional microscopy and molecular approaches including Denaturing Gradient Gel Electrophoresis (DGGE) and sequence analysis of 18S rRNA gene fragments. We compared eukaryotic assemblages of surface sediment biofilms collected in January, May and September 2002 from 13 sampling stations along the river. Physicochemical data revealed extremely acidic conditions (pH ranged from 0.9 to 2.5) with high concentrations of heavy metals including up to 20 g l-1 Fe, 317 mg l-1 Zn, 47 mg l-1 As, 42 mg l-1 Cd, and 4 mg l-1 Ni. In total, 20 taxa were identified, including members of the Bacillariophyta, Chlorophyta, and Euglenophyta phyla as well as ciliates, cercomonads, amoebae, stramenopiles, fungi, heliozoan and rotifers. In general, total cell abundances were highest in fall and spring decreasing drastically in winter and the sampling stations with the most extreme conditions showed the lowest number of cells as well as the lowest diversity. Species diversity does not vary much during the year. Only the filamentous algae showed a dramatic seasonal change almost disappearing in winter and reaching the highest biomass during the summer. PCA showed a high inverse correlation between pH and most of the heavy metals analyzed as well as Dunaliella sp., while Chlamydomonas sp. is directly related to pH during May and September. Three heavy metals (Zn, Cu and Ni) remained separate from the rest and showed an inverse correlation with most of the species analyzed except for Dunaliella sp.A.A was supported by the Spanish Ministry of Education and Science through the Ramón y Cajal program. This work has been supported by grant CGL2005-05470/BOS and grants to the Centro de Astrobiología at the Instituto National de Técnica Aeroespacial