Little is knownabout the functions of downstreamregulatory element antagonist modulator (DREAM) inembryonic
stem cells (ESCs). However, DREAM interacts with cAMP response element-binding protein (CREB) in a
Ca2+-dependent manner, preventing CREB binding protein (CBP) recruitment. Furthermore, CREB and CBP are
involved in maintaining ESC self-renewal and pluripotency. However, a previous knockout study revealed the
protective function of DREAMdepletion in brain aging degeneration and that aging is accompanied by a progressive
decline in stem cells (SCs) function. Interestingly, we found that DREAM is expressed in different cell types,
including human ESCs (hESCs), human adipose-derived stromal cells (hASCs), human bone marrow-derived
stromal cells (hBMSCs), and human newborn foreskin fibroblasts (hFFs), and that transitory inhibition of
DREAMin hESCs reduces their pluripotency, increasing differentiation.We stipulate that these changes are partly
mediated by increased CREB transcriptional activity. Overall, our data indicates that DREAMacts in the regulation
of hESC pluripotency and could be a target to promote or prevent differentiation in embryonic cells.Junta de Andalucía, Consejería de Innovación Ciencia y Empresa, FEDER CTS-6505; INP-2011- 1615-900000; P10-CVI-6095Instituto de Salud Carlos III, FEDER RD12/0019/0028; PI10/00964; PI14/0101
