Aspectos fisiológicos para venda e consumo de algumas frutas e hortaliças comercializadas na central de abastecimento do Pará (CEASA/PA) / Physiological aspects for the sale and consumption of some fruits and vegetables sold in the supply center of Pará (CEASA/PA)

 A Central de Abastecimento do Pará (CEASA/PA) é uma área de concentração física de frutas e hortaliças. O objetivo desde trabalho é avaliar os aspectos fisiológicos durante o consumo e comercialização de manga, abacaxi, couve-flor, cheiro-verde. Para esta finalidade foram aplicados dois questionários na CEASA/PA. Os resultados em relação ao perfil do consumidor mostram que o consumo de frutas e hortaliças está abaixo do recomendado pela OMS. O hábito e aparência são fatores determinantes para o consumo e compra das matrizes estudadas, além do menor preço. Quanto à mudança de hábitos alimentares os entrevistados mostram-se aptos às mudanças. Para a caracterização do vendedor e ponto de venda observou-se que o principal motivo para a comercialização de frutas e hortaliças é a experiência. No ponto de venda os entrevistados alegaram que não fazem nenhum tipo de tratamento pós-recepção das frutas e hortaliças. A maioria dos entrevistados vende de 100 a 150 kg/dia e utilizam transporte terceirizado e usam como principal estratégia de venda o bom atendimento e não realizam nenhum tipo de ação referente à melhoria na comercialização, tais como cursos e formação de cooperativas. Diante disso, o estudo apresenta informações importantes que possibilitam conhecer os perfis de consumo e venda desses produtos e os principais problemas enfrentados na comercialização, tornando possível verificar falhas e propor alternativas para aumentar o consumo e agregar valor às frutas e hortaliças

Physical training improves physical activity levels but is associated with amplification of sedentary behavior in older women

Physical activity level (PAL) and sedentary behavior (SB) are independent predictors of mortality. It is unclear how these predictors interact with each other and health variables. Investigate the bidirectional relationship between PAL and SB, and their impact and health variables of women aged 60 to 70 years. One hundred forty-two older adults women (66.3 ± 2.9 years) considered insufficiently active were submitted to 14 weeks of multicomponent training (MT), multicomponent training with flexibility (TMF), or the control group (CG). PAL variables were analyzed by accelerometry and QBMI questionnaire, physical activity (PA) light, moderate, vigorous and CS by accelerometry, 6 min walk (CAM), SBP, BMI, LDL, HDL, uric acid, triglycerides, glucose and cholesterol total. In linear regressions, CS was associated with glucose (B:12.80; CI:9.31/20.50; p < 0.001; R2:0.45), light PA (B:3.10; CI:2, 41/4.76; p < 0.001; R2:0.57), NAF by accelerometer (B:8.21; CI:6.74/10.02; p < 0.001; R2:0.62), vigorous PA (B:794.03; CI:682.11/908.2; p < 0.001; R2:0.70), LDL (B:13.28; CI:7.45/16.75; p < 0.002; R2:0.71) and 6 min walk (B:3.39; CI:2.96/8.75; p < 0.004; R2:0.73). NAF was associated with mild PA (B:0.246; CI:0.130/0.275; p < 0.001; R2:0.624), moderate PA (B:0.763; CI:0.567/0.924; p < 0.001; R2:0.745), glucose (B:−0.437; CI:−0.789/−0.124; p < 0.001; R2:0.782), CAM (B:2.223; CI:1.872/4.985; p < 0.002; R2:0.989) and CS (B:0.253; CI: 0.189/0.512; p < 0.001; R2:1.94). The NAF can enhance CS. Build a new look at how these variables are independent but dependent simultaneously, being able to influence the quality of health when this dependence is denied

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Catálogo Taxonômico da Fauna do Brasil: setting the baseline knowledge on the animal diversity in Brazil

The limited temporal completeness and taxonomic accuracy of species lists, made available in a traditional manner in scientific publications, has always represented a problem. These lists are invariably limited to a few taxonomic groups and do not represent up-to-date knowledge of all species and classifications. In this context, the Brazilian megadiverse fauna is no exception, and the Catálogo Taxonômico da Fauna do Brasil (CTFB) (http://fauna.jbrj.gov.br/), made public in 2015, represents a database on biodiversity anchored on a list of valid and expertly recognized scientific names of animals in Brazil. The CTFB is updated in near real time by a team of more than 800 specialists. By January 1, 2024, the CTFB compiled 133,691 nominal species, with 125,138 that were considered valid. Most of the valid species were arthropods (82.3%, with more than 102,000 species) and chordates (7.69%, with over 11,000 species). These taxa were followed by a cluster composed of Mollusca (3,567 species), Platyhelminthes (2,292 species), Annelida (1,833 species), and Nematoda (1,447 species). All remaining groups had less than 1,000 species reported in Brazil, with Cnidaria (831 species), Porifera (628 species), Rotifera (606 species), and Bryozoa (520 species) representing those with more than 500 species. Analysis of the CTFB database can facilitate and direct efforts towards the discovery of new species in Brazil, but it is also fundamental in providing the best available list of valid nominal species to users, including those in science, health, conservation efforts, and any initiative involving animals. The importance of the CTFB is evidenced by the elevated number of citations in the scientific literature in diverse areas of biology, law, anthropology, education, forensic science, and veterinary science, among others

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Contribution on the eating habits and new records of Mirothrips arbiter Cavalleri, Souza, Prezoto & Mound, 2013 (Thysanoptera: Phlaeothripidae) in Polistes Latreille, 1802 (Hymenoptera: Vespidae) wasp nests

New host wasps attacked by Mirothrips arbiter (Thysanoptera: Phlaeothripidae) are recorded and their possible feeding on fungi and mites in colonies of Polistes melanosoma and Polistes ferreri is evaluated. No predation events on mites or fungal ingestion were observed, indicating that these thrips only use wasp eggs as food. Thrips do not parasitize wasps’ bodies, they only live at the expense of their colonies, where they find food and shelter. However, as these insects feed on the immature stages of the wasps, it is possible that they cause or accelerate colony decline. Given this, we can say that the ecological relationship between thrips and social wasps is that of social parasitism, that is, thrips are parasites of wasps colonies, they depend directly on them for their survival and cause damage to them. From our findings, the number of host wasp species of this thrips rose to five, all belonging to the Polistinae subfamily

Avaliação da expressão de seis possíveis fatores de virulência de C. pseudotuberculosis em macrófagos murinos

Corynebacterium pseudotuberculosis é uma bacilo Gram-positivo causador da linfadenite caseosa em caprinos e ovinos. Dentre os seus possíveis fatores de virulência estão as proteínas pld (Fosfolipase D), cpp (Serina-protease CP40), nanH (Neuraminidase H), sodC (Superóxido Dismutase C), spaC (Adesina SpaC) e pknG (Proteína cinase G). Objetivo: este estudo buscou verificar, com a técnica qRT-PCR, se existe transcrição destes genes em macrófagos de duas linhagens de camundongos infectados in vitro com uma cepa viruleta C. pseudotuberculosis (CP1002). Paralelamente, também foi estudada a expressão dos genes pld, cpp, nanH, sodC e spaC nestas mesmas células. Método: extraiu-se o RNA total de macrófagos de camundongos C57BL/6 e Balb/c e sintetizou-se o DNA complementar ao RNA extraído. A detecção de RNA mensageiro de PknG, Pld, CP40, NanH, SodC e SpaC foi realizada por PCR quantitativa. Resultados: houve expressão dos seis genes nos macrófagos de camundongos das linhagens Balb/c e C57BL/6 infectados in vitro, nos três intervalos de tempo examinados (1, 12 e 24 horas após a infecção). A intensidade de transcrição dos genes pknG, pld, cpp, nanH, sodC e spaC uma hora após a infecção aparenta ser maior do que nos intervalos posteriores, tanto em macrófagos de Balb/c como em macrófagos de C57BL/6, embora nos primeiros todos os genes mostrem uma intensidade de transcrição supostamente mais acentuada neste intervalo. O gene pknG aparenta uma magnitude de transcrição mais intensa do que os demais, em ambos os tipos de animais. Conclusão: os genes pknG, pld, cpp, nanH, sodC e spaC são transcritos em macrófagos de Balb/c e de C57BL/6, expressando-se aparentemente de modo mais acentuado na primeira hora após a infecção em ambos os tipos de células. Todos os genes parecem exibir transcrição mais intensa em macrófagos dos animais da linhagem Balb/c

In vivo and in vitro expression of five genes involved in Corynebacterium pseudotuberculosis virulence

Abstract Caseous lymphadenitis (LC) is a chronic contagious disease caused by Corynebacterium pseudotuberculosis, which mainly affects goats and sheep. Vaccination is an effective but not yet well-established method, partly due to a lack of knowledge surrounding the most effective immunoprotective components. The present study aimed to quantify and compare the in vivo expression of genes pld (phospholipase D), cpp (CP40), nanH (neuraminidase H), sodC (superoxide dismutase C) and spaC (adhesin) using qRT-PCR, with the respective expression in vitro. Caseous material of abscesses removed from five animals was cultured, with colonies suggestive of C. pseudotuberculosis identified. RNA extraction was performed on these samples, as well as on the respective pellets derived from liquid cultures brain heart infusion. After evaluating RNA integrity, complementary DNA was synthesized, followed by the relative quantification each of the genes of interest. Mean mRNA expression of the five genes found in abscesses and in cultures differed significantly, with respective values of: nanH 811.50 ± 198.27 and 359.35 ± 75.45 (p = 0.009); cpp 856.31 ± 385.11 and 154.54 ± 94.34 (p = 0.0039); plD 922.70 ± 450.73 and 212.41 ± 153.10 (p = 0.016); sodC 1,293.53 ± 564.75 and 223.63 ± 145.58 (p = 0.016); spaC 1,157.10 ± 525.13 and 214.26 ± 125.70 (p = 0,016). Expression was observed to be 6–8 times higher in abscesses than in cultures, Indicative that is a genetic expression of the in vitro bacterium exists, yet in vivo has a greater magnitude corroborating to one of these virulence factors in the pathogenesis of LC

MOESM1 of In vivo and in vitro expression of five genes involved in Corynebacterium pseudotuberculosis virulence

Additional file 1. Linear, angular coefficients, regression equations and formulas for calculating the quantification factors in Table 1

Acute kidney injury and renal replacement therapy in critically ill COVID-19 patients: Risk factors and outcomes: A single-center experience in Brazil

Background: Critically ill patients with COVID-19 may develop multiple organ dysfunction syndrome, including acute kidney injury (AKI). We report the incidence, risk factors, associations, and outcomes of AKI and renal replacement therapy (RRT) in critically ill COVID-19 patients. Methods: We performed a retrospective cohort study of adult patients with COVID-19 diagnosis admitted to the intensive care unit (ICU) between March 2020 and May 2020. Multivariable logistic regression analysis was applied to identify risk factors for the development of AKI and use of RRT. The primary outcome was 60-day mortality after ICU admission. Results: 101 (50.2%) patients developed AKI (72% on the first day of invasive mechanical ventilation [IMV]), and thirty-four (17%) required RRT. Risk factors for AKI included higher baseline Cr (OR 2.50 [1.33-4.69], p = 0.005), diuretic use (OR 4.14 [1.27-13.49], p = 0.019), and IMV (OR 7.60 [1.37-42.05], p = 0.020). A higher C-reactive protein level was an additional risk factor for RRT (OR 2.12 [1.16-4.33], p = 0.023). Overall 60-day mortality was 14.4% {23.8% (n = 24) in the AKI group versus 5% (n = 5) in the non-AKI group (HR 2.79 [1.04-7.49], p = 0.040); and 35.3% (n = 12) in the RRT group versus 10.2% (n = 17) in the non-RRT group, respectively (HR 2.21 [1.01-4.85], p = 0.047)}. Conclusions: AKI was common among critically ill COVID-19 patients and occurred early in association with IMV. One in 6 AKI patients received RRT and 1 in 3 patients treated with RRT died in hospital. These findings provide important prognostic information for clinicians caring for these patients