New method to assess manual lymph drainage using lymphoscintigraphy

The aim of this study was to describe a new variation of thetechnique to evaluate lymph drainage utilizing lymphoscintigraphy.A LS scan marks the route of lymphatic vessels andmay be used to assess both manual lymph drainage and lymphdrainage after using some apparatuses. This evaluation may bedynamic, collecting images whilst performing lymph drainage orstatic, with scans before and after the lymph drainage procedure.The aim of this study was to describe a new variation of thetechnique to evaluate lymph drainage utilizing lymphoscintigraphy.A LS scan marks the route of lymphatic vessels andmay be used to assess both manual lymph drainage and lymphdrainage after using some apparatuses. This evaluation may bedynamic, collecting images whilst performing lymph drainage orstatic, with scans before and after the lymph drainage procedure

Central auditory processing in children after traumatic brain injury

Introduction: Traumatic brain injury can impair the central auditory pathways and auditory cortex. Hence, individuals who suffered a traumatic brain injury may be at risk of central auditory processing disorders, which can be identified with behavioral tests that assess central auditory function. Objective: To characterize and compare the performance of children and adolescents with and without a history of traumatic brain injury in behavioral tests that assess central auditory processing. Method: The sample comprised 8- to 18-year-old individuals of both sexes who suffered moderate or severe closed traumatic brain injury 3 to 24 months before their participation in the study and whose hearing thresholds were normal. These individuals were matched for sex and age with other subjects without a history of traumatic brain injury and submitted to behavioral assessment of the central auditory processing with special tests to assess hearing skills (namely, auditory closure, figure-ground, and temporal processing), selected according to their chronological age and response-ability. Results: The study group performed statistically worse than the comparison group in auditory closure, figure-ground in verbal dichotic listening, and temporal ordering. The central auditory processing tests with abnormal results in the comparison group were different from those in the study group. Conclusion: Central auditory processing disorders were identified in all subjects of the study group, especially involving auditory closure and temporal processing skills, in comparison with subjects without a history of traumatic brain injury

Expression analysis of a set of genes related to the ripening of bananas and mangoes

During ripening many important physic-chemical changes contribute to fruit quality, and they are precisely determined by gene expression. Specific genes are essential to normal ripening; however, information on gene expression about the majority of tropical fruit, such as bananas and mangoes is limited. In this way, the present study was undertaken with the objective to provide preliminary access to the changes in expression of some genes potentially relevant to banana and mango ripening. To simultaneously evaluate the changes in gene expression, a small collection of genes related to ethylene biosynthesis, starch mobilization, cell wall disassembly, pigment synthesis and ascorbate metabolism was assembled in nylon membranes and probed with cDNA from unripe and ripe fruit. Some interesting differences were observed between gene expression in bananas and mangoes. In relation to starch metabolism, banana a-amylase was induced during ripening while phosphorylase was more induced in mangoes. Similarly, expression of cell wall-related genes for polygalacturonase and expansin were also different in those fruits. Fructanfructosyltransferase, chalcone synthase, and ascorbate oxidase genes were also induced in ripening mangoes, but not in bananas. Although the number of sequences involved was relatively small, this simple and feasible approach provided interesting preliminary data that can be starting points for more in depth studies.FAPES

ESTÁGIO SUPERVISIONADO: PESQUISA DA PRÁTICA PEDAGÓGICA – GEOGRAFIA

O presente artigo busca discutir algumas indagações acerca da prática pedagógica e a formação inicial de professores, baseando-se na análise e na reflexão da intervenção realizada no campo de Estágio. Essas indagações surgem a partir da necessidade de se refletir sobre a prática em um movimento dialético com a teoria. Torna-se relevante trazer à tona as contribuições do Estágio Supervisionado como a superação  da visão dicotômica entre a teoria e a prática; a valorização da docência como atividade intelectual, crítica e reflexiva; a construção da identidade do professor frente o encontro e o confronto com a realidade escolar. O objetivo deste artigo não é esgotar as discussões sobre o tema, pelo contrário, é abrir um leque para novas discussões a partir da problematização da realidade vivenciada pelos acadêmicos ao longo da prática docente. Para construir o discurso sobre o fazer pedagógico buscou-se por meio da investigação e da pesquisa construir mecanismos para se avaliar e reavaliar a ação docente, ou seja, instrumentos de pesquisa como o questionário aplicado em momentos distintos para a avaliação da prática docente, com o propósito de perceber a construção da identidade do professor ao longo do curso de geografia. Como ponto de partida, foram utilizadas as reflexões sobre a prática pedagógica vivenciada no decorrer dos Estágios Supervisionados do 6º e do 7º período. Essas reflexões possibilitarão novas discussões e problematizações da prática pedagógica

O processo de expansão urbana e seus impactos na Estação Ecológica de Ribeirão Preto, SP

Although the normative instruments of urban planning include the environmental impact assessment, there is a loosening of these laws due to public and private interests that have purposes contrary to environmental policies. In the municipality of Ribeirão Preto, coffee and sugarcane monocultures have contributed to the fragmentation of native vegetation, reducing it to 6% of the municipal area (4,200 ha). The Ecological Station of Ribeirão Preto (ESRP) is the larger native remnant (155 ha) and suffers pressures for being located in the urban environment. This work investigated the urban pressure in the Buffer Zone (BZ) and its relation to the changes in the  Master Plan (MP) and complementary laws.  The land cover maps  (2010 and 2017) were also analyzed in order to quantify the conversion of native forests to anthropic uses, showing an intense urban expansion in the BZ over the period analyzed. On the other hand, municipal urban environmental legislation has evolved seeing that the previous MP (dated from 1995) approached the environment in a very specific way, which was modified in the 2018 law, which attempts to integrate the urban zoning to the buffer zone in order to make land use compatible with the ESRP Management Plan.Embora os instrumentos normativos de planejamento urbano incluam a avaliação de impacto ambiental, há uma flexibilização dessas leis devido a interesses públicos e privados que têm objetivos contrários às políticas ambientais. No município de Ribeirão Preto, a produção de café e cana-de-açúcar tem contribuído para a fragmentação da vegetação nativa, reduzida a 6% da área municipal (4.200 ha). A Estação Ecológica de Ribeirão Preto (EERP) é o maior remanescente nativo (155 ha) e sofre pressões por estar localizada no meio urbano. Este trabalho investigou a pressão urbana na Zona de Amortecimento (ZA) e sua relação com as mudanças no Plano Diretor (PD) e leis complementares. Os mapas de cobertura da terra (2010 e 2017) também foram analisados com o objetivo de quantificar a conversão de florestas nativas para usos antrópicos, mostrando uma intensa expansão urbana na ZA no período analisado. Por outro lado, a legislação urbanística-ambiental municipal evoluiu, visto que a MP anterior (datada de 1995) abordou o meio ambiente de maneira específica mas foi modificada na lei de 2018, que tenta integrar o zoneamento urbano à zona de amortecimento  a fim de tornar o uso do solo compatível com o Plano de Manejo da EERP

Electrospray Ionization Mass Spectrometry Fingerprinting of Extracts of the Leaves of Arrabidaea chica

Arrabidaea chica (crajiru) is an important Amazonian plant. Its extracts are used as red pigments, antimicrobial agents and astringents. Three different varieties of this species are cultivated in the Amazon region. In this work, direct infusions of A. chica extracts from these three varieties were analyzed via electrospray ionization mass spectrometry (ESI(+)-MS) fingerprinting. Derived data from the spectra were classified by using a multivariate method (PLS-DA, partial least squares-discriminant analysis). The direct method that is herein presented relies on extraction of dry, powdered leaves with acidified methanol/water solution with no further sample preparation. The resulting supernatants were analyzed by direct infusion ESI(+)-MS, which provides characteristic fingerprints of the sample composition. 3-Deoxyanthocyanidins are important substances in A. chica, their ions were used as markers in the PLS-DA data treatment. PLS-DA was able to differentiate the three varieties. ESI(+)-MS fingerprinting works as a simple and fast method to differentiate varieties of A. chica.2334090Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Electrospray ionization mass spectrometry fingerprinting of extracts of the leaves of arrabidaea chica

Arrabidaea chica (crajiru) is an important Amazonian plant. Its extracts are used as red pigments, antimicrobial agents and astringents. Three different varieties of this species are cultivated in the Amazon region. In this work, direct infusions of A. chica extracts from these three varieties were analyzed via electrospray ionization mass spectrometry (ESI(+)-MS) fingerprinting. Derived data from the spectra were classified by using a multivariate method (PLS-DA, partial least squares-discriminant analysis). The direct method that is herein presented relies on extraction of dry, powdered leaves with acidified methanol/water solution with no further sample preparation. The resulting supernatants were analyzed by direct infusion ESI(+)-MS, which provides characteristic fingerprints of the sample composition. 3-Deoxyanthocyanidins are important substances in A. chica, their ions were used as markers in the PLS-DA data treatment. PLS-DA was able to differentiate the three varieties. ESI(+)-MS fingerprinting works as a simple and fast method to differentiate varieties of A. chica233409414COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informaçãoSem informaçãoArrabidaea chica (crajiru) é uma importante planta da Amazônia. Seus extratos são usados como pigmento, agentes antimicrobianos e adstringentes. Três variedades diferentes desta espécie são cultivadas na região Amazônica. Neste trabalho, infusões diretas de extratos de A. chica destas três variedade foram analisadas por impressão digital via espectrometria de massas com ionização por eletrospray (ESI(+)-MS). Dados derivados destes espectros foram classificados utilizando um método de análise multivariada (PLS-DA, análise discriminante com calibração multivariada por mínimos quadrados parciais). O método direto aqui apresentado baseia-se na extração de folhas secas e em pó com uma solução de metanol/água acidificada sem manipulação adicional da amostra. A solução sobrenadante foi analisada através de infusão direta por ESI(+)-MS, o que resultou em perfis de composição para cada variedade. 3-Desoxiantocianidinas são importantes substâncias presentes na A. chica, seus íons foram usados como marcadores no tratamento de dados utilizando PLS-DA, o que possibilitou a separação das três variedades. Impressão digital por ESI(+)-MS funciona como um método simples e rápido para diferenciação de variedades de A. chic

The Bacterial and Fungal Gut Microbiota of the Greater Wax Moth, Galleria mellonella L. Consuming Polyethylene and Polystyrene

Plastic production has been increasing exponentially in the last 60 years, but plastic disposal is out of control, resulting in the pollution of all ecosystems on Earth. Finding alternative environmentally sustainable choices, such as biodegradation by insects and their associated gut microbiota, is crucial, however we have only begun to characterize these ecosystems. Some bacteria and one fungus have been previously identified in the gut of Greater Wax Moth larvae (Galleria mellonella L., Lepidoptera, Pyralidae) located mainly in the Northern hemisphere. The aim of this study was to describe changes in the gut microbiota associated with the consumption of polyethylene and polystyrene by the Greater Wax Moth in Argentina, considering both bacteria and fungi. Larvae were fed polyethylene, polystyrene and beeswax as control for 7 days. Next generation sequencing revealed changes in the bacterial gut microbiome of the wax moth larvae at the phyla and genus levels, with an increase in two Pseudomonas strains. The fungal communities showed no differences in composition between diets, only changing in relative abundance. This is the first report of both bacterial and fungal communities associated with a plastivore insect. The results are promising and call for more studies concerning a potential multi-kingdom synergy in the plastic biodegradation process.Fil: Ruiz Barrionuevo, Juliana María. Universidad Nacional de Tucumán. Instituto de Ecología Regional. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Ecología Regional; ArgentinaFil: Vilanova Cuevas, Brayan. Universidad de Puerto Rico; Puerto RicoFil: Alvarez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Martín, Eduardo. Universidad Nacional de Tucumán; Argentina. Fundación Miguel Lillo; ArgentinaFil: Malizia, Agustina. Universidad Nacional de Tucumán. Instituto de Ecología Regional. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Ecología Regional; ArgentinaFil: Galindo Cardona, Alberto. Fundación Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: de Cristobal, Ricardo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Occhionero, M. Angelica. Universidad Nacional de Tucumán; ArgentinaFil: Chalup, Adriana Elizabeth. Universidad Nacional de Tucumán; Argentina. Fundación Miguel Lillo; ArgentinaFil: Monmany, Ana Carolina. Universidad Nacional de Tucumán. Instituto de Ecología Regional. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Ecología Regional; ArgentinaFil: Godoy Vitorino, Filipa. Universidad de Puerto Rico; Puerto Ric