IMUNOHISTOCHEMTSTRY METHOD TO DETECT C-REACTIVE PROTEIN IN ATHEROMA PLAQUES OF SPRAGUE DAWLEY RATS FED HIGH LIPID RATION

Since Inflammation is believed to have role in pathogenesis of cardiovasculare vents, measurement of markers of inflammation has been proposed as a method to improve the prediction of the risk of these events. C-reactive protein (CRP) a major acute phase protein, has been associated with the presence and severity of atherosclerosis, and has been found to predict acute vasculare vents in prospective studies. The aim of this study was to analyse the relationship between appearance of CRP in aorta and atheroma plaque formation in Sprague Dawley rats after 59 days induction with high fat diet. Ten male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted in l0 single cages for 5 days and given basal diet containing normal fat and water ad libitum. After adaptation, rats were divided in to 2 groups (group 1 and group 2) of 5 each. Group I was fed basal diet containing normal fat(4.5% of fat) and group 2 was fed diet containing high fat (20% of fat). After 59 days, all rats were killed, the heart including aorta were taken out for histophatologic (HE) and immunohistochemistry analyses. The result of this study showed that all rats in Group 1 did not have atheroma plaque and CRP negative, but rats in G"cup 2, all have atheroma plaque (100%) but only 60% CRP positive. From those result it concluded that although all aortas have atheroma plaque, the CRP may not be detected in the plaque.Keywords: atheroma plaques, inflammation, C-reactive protein

Imunohistochemtstry Method to Detect C-reactive Protein in Atheroma Plaques of Sprague Dawley Rats Fed High Lipid Ration

Since Inflammation is believed to have role in pathogenesis of cardiovasculare vents, measurement of markers of inflammation has been proposed as a method to improve the prediction of the risk of these events. C-reactive protein (CRP) a major acute phase protein, has been associated with the presence and severity of atherosclerosis, and has been found to predict acute vasculare vents in prospective studies. The aim of this study was to analyse the relationship between appearance of CRP in aorta and atheroma plaque formation in Sprague Dawley rats after 59 days induction with high fat diet. Ten male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted in l0 single cages for 5 days and given basal diet containing normal fat and water ad libitum. After adaptation, rats were divided in to 2 groups (group 1 and group 2) of 5 each. Group I was fed basal diet containing normal fat(4.5% of fat) and group 2 was fed diet containing high fat (20% of fat). After 59 days, all rats were killed, the heart including aorta were taken out for histophatologic (HE) and immunohistochemistry analyses. The result of this study showed that all rats in Group 1 did not have atheroma plaque and CRP negative, but rats in G"cup 2, all have atheroma plaque (100%) but only 60% CRP positive. From those result it concluded that although all aortas have atheroma plaque, the CRP may not be detected in the plaque

The Effect of High Lipid Diet Lipopolysaccharide and Laparatomy Surgery on the Level of C-reactive Protein Serum in Sprague Dawley Rats Ekd

C-reactive protein (CRP) is a member of the family of proteins known as pentraxins, and originally defined as a substance, observed in the serum or plasma of patient with acute infections, that reacted with the Cpolysaccharide of the pneumococcus. Serum concentrations of CRP increase as part of the inflammatory response to infection or acute injury. The aim of the study was to see the differences between acute and chronicstimuli in the increasing of CRP concentration in serum of Sprague Dawley rat. Twenty male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted for 5 days and given basal diet,containing normal fat(5% of fat) and water ad libitum. Rats were then divided into 4 groups of 5 each. Group 1 was used as control animal. The animals in this group were fed basal diet for 59 days without any othertreatments. Group 2 was animals fed high fat diet containin g20Yo of fat, for 59 days without any other treatments. Group 3 was animals fed basal diet for 59 days and in the day of 60 then injected with LPS from Escherichia coli 0111:B4, 1 mg/kg BW intraperitoneally, and Group 4 was animals fed basal diet for 59 days and in the day of 60 animals then laparatomized. Twenty four hours after all treatments, blood sample were collected from orbitally vein, serum was separated and used to analyze CRP concentration. Statistical analyzes using onew ay analyzeso fvariances howed that there were significance differences among Group 1,2,3 and 4 (p<0.05). Mean of CRP concentrations respectively from the highest to the lowest were: Group 3, Group 4, Group 2 and Group 1. Fromthe result of the study it can be concluded that CRP concentration of acute stimulus was higher than chronic stimulus

THE EFFECT OF HIGH LIPID DIET LIPOPOLYSACCHARIDE AND LAPARATOMY SURGERY ON THE LEVEL OF C-REACTIVE PROTEIN SERUM IN SPRAGUE DAWLEY RATS EKD

C-reactive protein (CRP) is a member of the family of proteins known as pentraxins, and originally defined as a substance, observed in the serum or plasma of patient with acute infections, that reacted with the Cpolysaccharide of the pneumococcus. Serum concentrations of CRP increase as part of the inflammatory response to infection or acute injury. The aim of the study was to see the differences between acute and chronicstimuli in the increasing of CRP concentration in serum of Sprague Dawley rat. Twenty male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted for 5 days and given basal diet,containing normal fat(5% of fat) and water ad libitum. Rats were then divided into 4 groups of 5 each. Group 1 was used as control animal. The animals in this group were fed basal diet for 59 days without any othertreatments. Group 2 was animals fed high fat diet containin g20Yo of fat, for 59 days without any other treatments. Group 3 was animals fed basal diet for 59 days and in the day of 60 then injected with LPS from Escherichia coli 0111:B4, 1 mg/kg BW intraperitoneally, and Group 4 was animals fed basal diet for 59 days and in the day of 60 animals then laparatomized. Twenty four hours after all treatments, blood sample were collected from orbitally vein, serum was separated and used to analyze CRP concentration. Statistical analyzes using onew ay analyzeso fvariances howed that there were significance differences among Group 1,2,3 and 4 (p<0.05). Mean of CRP concentrations respectively from the highest to the lowest were: Group 3, Group 4, Group 2 and Group 1. Fromthe result of the study it can be concluded that CRP concentration of acute stimulus was higher than chronic stimulus.Key words : C-reactivep rotein, lipopolysaccharides, high fat diet, laparatomy

The Analysis of Total Leucocyte, C-Reactive Protein and Fibrinogen Concentrations to Evaluate the Leakage of Enterectomy Result

Enterectomy is an operative method that has very dangerous risk. The purpose of this research was done to see the physiological view after surgery, especially in the total leucocyte, C-reactive protein (CRP) andfibrinogen concentrations. Nine female, healthy local dogs, 10 kgs of body weight were used as experimental study. The dogs were adapted for a week into individual cages and fed commercial dog-food and water adlibitum. In the day of 7, all dogs were fasted 12 hours for anaesthesia preparation. The dogs were then divided into 3 Groups of 3 each. Group I was used as control, it had normal enterectomy surgery. Group II was animals that had enterectomy surgery with one hole leakage. Group III was animals that had enterectomy surgery with two special hole leakages. Enterectomy itself was done on to jejunum with 5 cm of gut should be cut away. Anastomosis method was done using end to end method and interrupted suturing method with chromic cat gut 0/3. The result of the research showed that there is similarly increasing level of leucocyte and CRP in the second day after surgery and decreased gradually until the sixth day, but different type of changes in fibrinogen level. The increasing level of CRP has advantages, such as it can be used as eidker marker of inflammation or infection, or worse condition after enterectomy

The Analysis of Total Leucocyte, C-Reactive Protein and Fibrinogen Concentrations to Evaluate The Leakage of Enterectomy Result

Enterectomy is an operative method that has very dangerous risk. The purpose of this research was done to see the physiological view after surgery, especially in the total leucocyte, C-reactive protein (CRP) andfibrinogen concentrations. Nine female, healthy local dogs, 10 kgs of body weight were used as experimental study. The dogs were adapted for a week into individual cages and fed commercial dog-food and water adlibitum. In the day of 7, all dogs were fasted 12 hours for anaesthesia preparation. The dogs were then divided into 3 Groups of 3 each. Group I was used as control, it had normal enterectomy surgery. Group II was animals that had enterectomy surgery with one hole leakage. Group III was animals that had enterectomy surgery with two special hole leakages. Enterectomy itself was done on to jejunum with 5 cm of gut should be cut away. Anastomosis method was done using end to end method and interrupted suturing method with chromic cat gut 0/3. The result of the research showed that there is similarly increasing level of leucocyte and CRP in the second day after surgery and decreased gradually until the sixth day, but different type of changes in fibrinogen level. The increasing level of CRP has advantages, such as it can be used as eidker marker of inflammation or infection, or worse condition after enterectomy.Keywords : enterectomy, leucocyte, C-reactive protein, fibrinogen, inflammatio

Profil Darah Rusa Totol (Axis axis) Betina Sehat di Pusat Inovasi Agroteknologi (PIAT), Universitas Gadjah Mada

Center for Agrotechnology Innovation, Gadjah Mada University (PIAT), one of the place for development and breeding ground of spotted deer in Yogyakarta, Indonesia. The study of blood profiles was intended to allow veterinarian to understand the normal profile of the spotted deer. Fifteen healthy spotted deer belonging to PIAT were used as experimental animals. The deer were drawn its blood through the jugular vein without the use of anaesthesia. Blood was then accommodated in an EDTA tube, centrifuged at 2500 RPM and analyzed using a Mindray BC-2800 haematology analyzer machine. Based on the results of the study of the blood it were known that  Haemoglobine (Hb) was: 11.5 ± 1.703 g/dl, Red blood cells (RBC) was: 9.3 ± 3.580 106/ml, Packed cell volume (PCV) was: 30.8 ± 6.035 %, Mean corpuscular Volume (MCV) was: 36.8 ± 11.102 fl, Mean corpuscular haemoglobine (MCH) was: 15.0 ± 7.313 pg, Mean corpuscular haemoglobine concentration (MCHC) was: 40.0 ± 14.657 g/dl, White blood cells (WBC) was: 6.4 ± 3.096 103/ml , Neutrophils was: 43.4 ± 21.646, % Basophils was: 0.2 ± 0.168 %, eosinophils was: 0.4 ± 0.447 %, lymphocytes was: 53.4 ± 21.546 % and monocytes was: 2.6 ± 2.394 %. From all of the datas it indicated that blood profiles of PIAT’s spotted deers are different from similar spotted deer blood profiles in India

Analisis Leukosit Total, C-Reactive Protein (CRP) dan Fibrinogen untuk Evaluasi Kebocoran Basil Operasi Enterektomi

Enterectomy is an operative method that has very dangerous risk. The purpose of this research was done to see the physiological view after surgery, especially in the total leucocyte, C-reactive protein (CRP) and fibrinogen concentrations. Nine female, healthy local dogs, 10 kgs of body weight were used as experimental study. The dogs were adapted for a week into individuaf cages and fed commercial dog-food and water ad libitum. In the day of 7, all dogs were fasted 12 hours for anaesthesia preparation. The dogs were then divided into 3 Groups of 3 each. Group I was used as control, it had normal enterectomy surgery. Group II was animals that had enterectomy surgery with one hole leakage. Group III was animals that had enterectomy surgery with two special hole leakages. Enterectomy itself was done on to jejunum with 5 cm of gut should be cut away. Anastomosis method was done using end to end method and interrupted suturing method with chromic cat gut 3. The result of the research showed that there is similarly increasing level ofleucocyte and CRP in the second day after surgery and decreased gradually until the sixth day, but different type of changes in fibrinogen level. The increasing level of CRP has advantages, such as it can be used as eidker marker of inflammation or infection, or worse condition after enterectomy

Expression of Bone Morphogenetic Protein-2 ForMeasuring Effectiveness of Freeze Dried Bovine Bone Xenografts (FDBBX) Biomaterials as Bone ConnectorMaterial

Osteogenic activity in normal fracture healing will start early at around 1 week after the repair , but not necessarily occur in fracture healing with the aid of grafts. Demineralized freeze dried bovine bone xenograft (DFDBBX) is a bone graft material made from cows that are widely produced recently to assist the process of bone grafting in humans, but has not been used on animals. It does not know how it works in helping to repair the bone. T wenty male SD rats weighing 150 g, were divided into 2 groups of 10 rats each. Group I was the control group, the rats were fractured and reconnected immediately using intramedullar pin fixation, Group II was a group of rats that were fractured and reconnected immediately using pin fixation intramedular plus graft material of freeze dried bovine bone xenograft. Fracture of the left femur was made in diaphysis part of bone. Evaluation of joints performed regularly at 1 week , 2 weeks, 4 weeks, 6 weeks and 8 weeks post surgery respectively by selecting at random, each group of 2 rats. Rats at each observation were collected two head / group, rats were then killed, the bone parts to be joined was taken and stored in 10% of formalin for histopathologic examination. Some pieces of the bones were used for the analysis of bone morphogenetic protein 2 (BMP-2 ) by immunohistochemical method. The results were then analyzed descriptively to see the changes directly on the X-rays and tissue. Overall results of this study showed that the graft material of DFDBBX were osteocondusive rather than osteoinductive properties. Nevertheless, the biomaterial is useful for orthopedic purposes primarily of handling cases with partial fracture of bone tissue which is lost. Due to the nature osteocondusive of DFDBBX will make cells to grow and to unify the separate bone fragments.Keywords : fracture, osteogenic activity , demineralized freeze dried bovine bone xenograft, bone morphogenetic protein-2, immunohistochemical analysi