Gadjah Mada University

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    The exploration of dominant spoilage bacteria in blue mussels (Mytilus edulis) stored under different modified atmospheres by MALDI-TOF MS in combination with 16S rRNA sequencing

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    Few studies have addressed species-level identification of spoilage bacteria in blue mussels packed under modified atmospheres (MAs). We investigated the effect of MAs and seasons on the tentative species-level of dominant spoilage bacteria in blue mussels. Summer (s) and winter (w) blue mussels were stored at 4 °C in the atmospheres (CO2/O2/N2): A40s (30/40/30), B60s (40/60/0), C60s (0/60/40), A40w (30/40/30), and D75w (25/75/0). In total, 122 culturable isolates were obtained at the final stage of shelf life, when mortality was high (56–100) and total psychrotrophic bacteria counted >7 log CFU g−1. Biochemical properties were analyzed using gram reactions, catalase and oxidase activities, and salt tolerance tests. Culturable isolates were identified through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16 S rRNA gene sequence analysis. Spoilage potential tests were investigated by evaluating protease, lipase, and fermentation activities as well as gas and H2S production. The culturable isolates showed tolerance to varied salt concentrations. Psychromonas arctica, Pseudoalteromonas elyakovii, and Shewanella frigidimarina were dominating in specific MAs. Winter blue mussels resulted in a higher variation of spoilage bacteria, including S. frigidimarina, S. vesiculosa, S. polaris, Micrococcus luteus, Paeniglutamicibacter terrestris sp. nov., and Alteromonas sp. © 2023 Elsevier Lt

    Local Community Readiness to Implement Smart Tourism Destination in Yogyakarta, Indonesia

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    Local economic development for relatively long period of time. Tourism also allows rapid recovery of local economy after COVID-19 outbreak. Amongst different kinds of approaches for tourism development, smart tourism destination (STD) has been widely believed as an excellent approach to rapidly recover tourism sector during post-pandemic. STD concept also allows improvement of adaptive capacity that is critical to anticipate any future disturbances including the unpredicted ones. However, a question remains regarding the readiness of local communities for implementing STD. Therefore, this research aims to address this gap by examining the readiness of managers of community-based tourism in the Special Region of Yogyakarta province, Indonesia. An online survey was conducted to collect data from various community-based tourism destinations located in this region. Data were analyzed using descriptive statistics to measure the overall readiness of community-based tourism to implement STD. For triangulation purpose, interviews were conducted to complement the online survey. © The Author(s), under exclusive license to Springer Nature Switzerland AG. 2024

    Estimating finger joint angles by surface EMG signal using feature extraction and transformer-based deep learning model

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    Human-machine interfaces frequently use electromyography (EMG) signals. Based on previous work, feature extraction has a great deal of influence on the performance of EMG pattern recognition. Furthermore, the Deep Learning method is supposed to increase performance and not depend on feature engineering. However, directly processing raw signals will require a higher computation rate. This study proposed a new method that combines feature extraction and Deep Learning to address those issues while improving performance, reducing architecture size, and producing a more representative output. The proposed architecture employs the Transformer model as the backbone to get the correlation between elements and focus on the important information for estimating the flexion-extension of finger joint angles. This study uses experiment three of the NinaPro (Non-Invasive Adaptive Hand Prosthetics) DB5 dataset. Each experiment produces 16 Surface EMG data streams from two Myo Armbands devices representing 22 finger joint angles as output. This study compares the windowing process, feature extraction, execution time, and results with previous studies. The results show that the proposed model outperforms the previous study, from 0.957 in the previous study to become 0.970 in this study for the R-Square score. This result is obtained using 100 data points for the windowing process and Median Frequency for the best feature extraction method. © 202

    Long-term conversion of upland to paddy increased SOC content and N availability in a sand dune of Japan

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    Land use change driven by anthropogenic activities has greatly affected terrestrial C and N cycling; however, it remains unclear how dynamics of soil organic carbon (SOC) and nitrogen (N) availability respond to long-term land use change in sand dunes. Here, we investigated the changes in SOC, total nitrogen (TN), and their aerobic and anaerobic decomposition or mineralization in a vinyl film paddy, compared with dry land use and management changes (LUMCs) (original upland, greenhouse, and agricultural forest (tree area)) in Shonai sand dunes, northeast Japan. The vinyl film paddy had been singly cultivated for 54 years. Soil samples were collected from five soil depths (0�10, 10�20, 20�30, 30�40, 40�50 cm) to compare the effects of depth and LUMC among the four treatments. The C decomposition (Dec-C) was assessed as the aerobic CO2 production and anaerobic methane and CO2 productions. Meanwhile, N mineralization (Min-N) was determined as aerobic NH4+ and NO3� production and anaerobic NH4+ production. The results showed that the vinyl film paddy increased SOC and TN contents, Dec-C and Min-N than upland and greenhouses at 0�30 cm depth but lower levels than the tree area at all depths. The arithmetic mean for all depths showed the aerobic Dec-C in the paddy (216.0 mg kg�1) was higher than the corresponding anaerobic Dec-C (192.8 mg kg�1). Conversely, the aerobic Min-N (35.9 mg kg�1) was lower than the anaerobic Min-N (75.7 mg kg�1). There were significantly positive correlations among SOC, TN, aerobic and anaerobic Dec-C and Min-N, whereas the paddy had steeper slope than other three LUMCs. Conclusively, our results indicated that long-term conversion of upland to vinyl film paddy increased SOC and TN accumulation and N availability (Min-N) in Shonai sand dunes, which can be applied as an effective agricultural practice to improve fertility of coastal sandy soils. © 2023 Elsevier B.V

    Related party lending and rural bank risk: Evidence during the Covid-19 period

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    This study aims to investigate bank lending behaviors during the Covid-19 period and further to examine the effect of related party lending (RPL) on the rural bank risk. We posit there is significant change on the lending growth and related party lending proportion during this period. We utilize 730 rural banks in Indonesia using panel data approach from 2019 to 2022. Utilizing panel data estimations to test the impact of RPL on risk and to further investigate its interaction with the COVID-19 using system GMM, we document that RPL is negatively associated with rural bank risk proxied by loan loss provision as ex-ante credit risk and positively associated with Z-Score as bank default risk. Moreover, the COVID-19 weaken the relationship between related party lending and rural bank risk. These results provide new insight into understanding risk management implementation for minimizing these risks. We also adopt several proxies and a split sample analysis to check for the robustness. Finally, we seek for lesson learned from the crisis and propose some implication for bank and relevant authorities. © 2023 Elsevier B.V

    Identification of early flowering mutant gene in Phalaenopsis amabilis (L.) Blume for sgRNA construction in CRISPR/Cas9 genome editing system

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    Phalaenopsis amabilis (L.) Blume commonly called Moth Orchid (Orchidaceae) is a natural orchid species designated as the National Flower of Indonesia for its beautiful flower shape and long-lasting flowering period. Basically, P. amabilis has a long vegetative phase that cause late flowering, about 2 to 3 years for flowering, hence a method to shorten vegetative period is desired. The latest technological approach that can be used to accelerate flowering of P. amabilis is the CRISPR/Cas9 genome editing method to inactivate the GAI (Gibberellic Acid Insensitive) gene as a mutant gene that can accelerate the regulation of FLOWERING TIME (FT) genes flowering biosynthesis pathway. The approach that needs to be taken is to silence the GAI gene with a knockout system which begins with identifying and characterizing the GAI target gene in the P. amabilis which will be used as a single guide RNA. CRISPR/Cas9 mediated knockout efficiency is highly dependent on the properties of the sgRNA used. SgRNA consists of a target sequence, determining its specificity performance. We executed phylogenetic clustering for the PaGAI protein with closely related orchid species such as Dendrobium capra, Dendrobium cultivars and Cymbidium sinensis. SWISS-Model as tool webserver for protein structure homology modeling. Results show that P. amabilis has a specific domain with the occurrence of point mutations in the two conservative domains. Therefore, a single guide RNA reconstruction needs to be implemented. © 2024, Instituto Internacional de Ecologia. All rights reserved

    Denying the accusation of plagiarism: power relations at play in dictating plagiarism as academic misconduct

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    In academia, plagiarism is considered detrimental to the advancement of sciences, and the plagiarists can be charged with sanctions. However, the plagiarism cases involving three rectors of universities in Indonesia stand out, as they could defend their stand for not committing academic misconduct despite evidence found. By analyzing the three rectors' cases, the present study aims to answer how power relations take a role in plagiarism discourse in Indonesia, particularly in determining what is considered academic misconduct and what is not. By employing critical discourse analysis, we found that when the accusation of plagiarism appears during rectorial elections, the accused could equivocate that the accusation was meant to undermine them as a political opponent. When the accused plagiarists win the election, they have more power to deny and tackle the accusations of plagiarism. The findings indicate that plagiarism issues can be politicized, in which by those in power it can be used as a tool to undermine their political opponents, whereas the accused plagiarists can claim that the actual problem is personal and not about plagiarism. It is also shown that in the real context, whether something is called plagiarism or not is subject to interpretation by those in power

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