Assessing and Analyzing Bat Activity with Acoustic Monitoring: Challenges and Interpretations

Acoustic monitoring is a powerful technique for learning about the ecology of bats, but understanding sources of variation in the data collected is important for unbiased interpretation. The objectives of this dissertation were to investigate sources of variation in acoustic monitoring and make recommendations for acoustic survey design and analysis. I addressed this goal in three ways: i) variation resulting from differences in bat detectors, ii) methods for objective identification of peak activity, and iii) the use of stationary transects to address within-site spatial variation. First, I compared variation of detection of echolocation calls among commonly available bat detectors and found significant differences in distance and angle of detection. Consequently, this source of variation should be taken into account when comparing datasets obtained with different systems. Furthermore, choice of detector should be taken into account when designing new studies. Second, I investigated two statistical methods for identifying peaks in activity, percentile thresholds and space-time scan statistic (SaTScan). Acoustic monitoring provides a relative measure of activity levels and is rarely evaluated based on objective criteria, so describing bat activity as “high” or “low” is useful only in context of the studies in question. Percentile thresholds allow for peaks to be identified relative to a larger distribution of activity levels. SaTScan identifies peaks in space and time that are significantly higher than the background expectation of the dataset. Both methods are valuable tools for replicable and objective identification of peak activity that can be applied at various temporal and spatial scales. Third, I examine how within-site spatial variation can impact estimates of bat activity. I used a stationary transect of bat detectors to i) assess variation in patterns of activity at each detector, ii) test whether spatial or temporal factors were more important for explaining variation in activity, iii) explore what sampling effort in space and time is required for species-specific activity levels. The picture of activity differs significantly within a site depending on detector placement so it is important to use multiple detectors simultaneously to collect accurate estimates of activity

Supporting service change in palliative care: a framework approach

Copyright 2014 Mark Allen Healthcare. editorially accepted manuscript version of the paper reproduced here with permission from the publisher.Background Palliative care services are increasingly identifying areas for improvement, then trying to create appropriate changes in response. Nurses in particular are often expected to take leading roles in Quality Improvement (QI) but are not necessarily trained or supported in these processes. Methods. A framework approach to change was developed to guide services through a change cycle, and delivered via workshops by representatives of three national projects in Australia. Participants were predominantly nurses (80%) with the majority over the age of 50 (62.4%). The workshops and the framework were positively evaluated with participants feeling confident in a number of QI related activities following workshop training. Discussion. Recognising and addressing problems in clinical practice and service delivery is an important way for nurses to ensure quality care for patients, however, they need support in developing the skills and knowledge that are essential to successful QI activities

Association of SNPs in LCP1 and CTIF with hearing in 11 year old children:findings from the Avon Longitudinal Study of Parents and Children (ALSPAC) birth cohort and the G-EAR consortium

BACKGROUND: The genetic basis of hearing loss in humans is relatively poorly understood. In recent years, experimental approaches including laboratory studies of early onset hearing loss in inbred mouse strains, or proteomic analyses of hair cells or hair bundles, have suggested new candidate molecules involved in hearing function. However, the relevance of these genes/gene products to hearing function in humans remains unknown. We investigated whether single nucleotide polymorphisms (SNPs) in the human orthologues of genes of interest arising from the above-mentioned studies correlate with hearing function in children. METHODS: 577 SNPs from 13 genes were each analysed by linear regression against averaged high (3, 4 and 8 kHz) or low frequency (0.5, 1 and 2 kHz) audiometry data from 4970 children in the Avon Longitudinal Study of Parents and Children (ALSPAC) birth-cohort at age eleven years. Genes found to contain SNPs with low p-values were then investigated in 3417 adults in the G-EAR study of hearing. RESULTS: Genotypic data were available in ALSPAC for a total of 577 SNPs from 13 genes of interest. Two SNPs approached sample-wide significance (pre-specified at p = 0.00014): rs12959910 in CBP80/20-dependent translation initiation factor (CTIF) for averaged high frequency hearing (p = 0.00079, β = 0.61 dB per minor allele); and rs10492452 in L-plastin (LCP1) for averaged low frequency hearing (p = 0.00056, β = 0.45 dB). For low frequencies, rs9567638 in LCP1 also enhanced hearing in females (p = 0.0011, β = -1.76 dB; males p = 0.23, β = 0.61 dB, likelihood-ratio test p = 0.006). SNPs in LCP1 and CTIF were then examined against low and high frequency hearing data for adults in G-EAR. Although the ALSPAC results were not replicated, a SNP in LCP1, rs17601960, is in strong LD with rs9967638, and was associated with enhanced low frequency hearing in adult females in G-EAR (p = 0.00084). CONCLUSIONS: There was evidence to suggest that multiple SNPs in CTIF may contribute a small detrimental effect to hearing, and that a sex-specific locus in LCP1 is protective of hearing. No individual SNPs reached sample-wide significance in both ALSPAC and G-EAR. This is the first report of a possible association between LCP1 and hearing function

Efficacy of CMX001 as a Prophylactic and Presymptomatic Antiviral Agent in New Zealand White Rabbits Infected with Rabbitpox Virus, a Model for Orthopoxvirus Infections of Humans

CMX001, a lipophilic nucleotide analog formed by covalently linking 3-(hexdecyloxy)propan-1-ol to cidofovir (CDV), is being developed as a treatment for smallpox. CMX001 has dramatically increased potency versus CDV against all dsDNA viruses and, in contrast to CDV, is orally available and has shown no evidence of nephrotoxicity in healthy volunteers or severely ill transplant patients to date. Although smallpox has been eliminated from the environment, treatments are urgently being sought due to the risk of smallpox being used as a bioterrorism agent and for monkeypox virus, a zoonotic disease of Africa, and adverse reactions to smallpox virus vaccinations. In the absence of human cases of smallpox, new treatments must be tested for efficacy in animal models. Here we first review and discuss the rabbitpox virus (RPV) infection of New Zealand White rabbits as a model for smallpox to test the efficacy of CMX001 as a prophylactic and early disease antiviral. Our results should also be applicable to monkeypox virus infections and for treatment of adverse reactions to smallpox vaccination

Brown Treesnake Mortality After Aerial Application of Toxic Baits

Quantitative evaluation of control tools for managing invasive species is necessary to assess overall effectiveness and individual variation in treatment susceptibility. Invasive brown treesnakes (Boiga irregularis) on Guam have caused severe ecological and economic effects, pose a risk of accidental introduction to other islands, and are the greatest impediment to the reestablishment of extirpated native fauna. An aerial delivery system for rodent‐based toxic baits can reduce brown treesnake abundance and heterogeneity among individuals may influence bait attraction or toxicant susceptibility. Previous baiting trials have either been simulated aerial treatments or relied on slightly different bait capsule compositions and the results of aerial delivery of toxic baits under operational conditions may not be directly comparable. We monitored 30 radio‐tagged adult snakes (990–1,265 mm snout‐vent length) during an aerial baiting operation in a 55‐ha area using transmitters equipped with accelerometers and receivers programed to display a status code indicating mortality if a snake failed to move for \u3e24 hours. We used known‐fate models to estimate mortality and evaluate a priori hypotheses explaining differences in mortality based on size, sex, and treatment effects. Eleven radio‐tagged snakes died in the aerial baiting treatment period (0.37, 95% CI=0.21–0.55) and no individuals (0.00, 95% CI=0.00–0.04) died during the non‐treatment period. Our data provide strong evidence for an additive size‐based treatment effect on mortality, with smaller adults (0.59, 95% CI=0.35–0.80) exhibiting higher mortality than larger snakes (0.14, 95% CI=0.02–0.37) but did not support a sex effect on mortality. The high mortality of snakes during the treatment period indicates that aerial baiting can reduce brown treesnake abundance, but further refinement or use in combination with other removal tools may be necessary to overcome size‐based differences in susceptibility and achieve eradication. © 2021 The Authors. The Journal of Wildlife Management published by Wiley Periodicals LLC on behalf of The Wildlife Society

Bacterial genomics reveal the complex epidemiology of an emerging pathogen in Arctic and boreal ungulates

Northern ecosystems are currently experiencing unprecedented ecological change, largely driven by a rapidly changing climate. Pathogen range expansion, and emergence and altered patterns of infectious disease, are increasingly reported in wildlife at high latitudes. Understanding the causes and consequences of shifting pathogen diversity and host-pathogen interactions in these ecosystems is important for wildlife conservation, and for indigenous populations that depend on wildlife. Among the key questions are whether disease events are associated with endemic or recently introduced pathogens, and whether emerging strains are spreading throughout the region. In this study, we used a phylogenomic approach to address these questions of pathogen endemicity and spread for Erysipelothrix rhusiopathiae, an opportunistic multi-host bacterial pathogen associated with recent mortalities in arctic and boreal ungulate populations in North America. We isolated E. rhusiopathiae from carcasses associated with large-scale die-offs of muskoxen in the Canadian Arctic Archipelago, and from contemporaneous mortality events and/or population declines among muskoxen in northwestern Alaska and caribou and moose in western Canada. Bacterial genomic diversity differed markedly among these locations; minimal divergence was present among isolates from muskoxen in the Canadian Arctic, while in caribou and moose populations, strains from highly divergent clades were isolated from the same location, or even from within a single carcass. These results indicate that mortalities among northern ungulates are not associated with a single emerging strain of E. rhusiopathiae, and that alternate hypotheses need to be explored. Our study illustrates the value and limitations of bacterial genomic data for discriminating between ecological hypotheses of disease emergence, and highlights the importance of studying emerging pathogens within the broader context of environmental and host factors

Dataset associated with "Seeking congruity for agentic women: a longitudinal examination of college women's persistence in STEM"

Those interested in using these data are encouraged to contact Dr. Paul Hernandez ([email protected]) and Dr. Emily Fischer ([email protected]) for more information. Data Contacts: Paul R. Hernandez (primary), [email protected], 979-464-9229 Emily V. Fischer, [email protected], 970-491-8587.Survey data were collected via the online Qualtrics survey system twice yearly in the fall and spring semesters from fall 2015 through spring 2019. This repository contains the data file associated with all surveys utilized in the analyses presented in this research article.Format of Data Files: Data files are in .csv format. Files can be opened by most software (e.g., Notepad, WordPad, Excel) – anything that can read a comma delimited ASCII text file. Here, the file name is "Dataset." In addition, a codebook accompanies the data file. The codebook contains the variable names, variable labels, and value labels for all variables contained within the "Dataset.csv" file.An abundance of literature has examined barriers to women's equal representation in science, technology, engineering, and math (STEM) fields, with many studies showing that STEM fields are not perceived to afford communal goals, a key component of women's interest in future careers. Using Goal Congruity Theory as a framework, we tested the longitudinal impact of perceptions of STEM career goal affordances, communal and agentic goals, and their congruity on persistence in science from the second through fourth years of college among women in STEM majors. We found that women's intent to persist in science were highest in fall of their second year, that persistence intentions exhibited a sharp decline, and that eventually leveled off by their fourth year of college. This pattern was moderated by perceptions of agentic affordances in STEM, such that women with higher perceptions of agentic affordances experienced smaller declines. Similar to prior research, we found that higher perceptions of communal goal affordances in STEM consistently predicted higher persistence intentions. Finally, we found an agentic goal-affordance congruity interaction, such that higher perceptions of agentic affordances in STEM predicted higher persistence intentions; however, the positive relationship was stronger for women with higher agentic goals. We conclude that because STEM fields are stereotyped as affording agentic goals, women who identify interest in a STEM major during their first year of college may be drawn to these fields for this reason, and may benefit from perceptions that STEM affords both communal and agentic goals.Funding for this work was provided by the National Science Foundation through grant number DUE- 1431795, DUE-1431823, and DUE-1460229

Role modeling is a viable retention strategy for undergraduate women in the geosciences

Gender diversity leads to better science; however, a number of science, technology, engineering, and mathematics (STEM) disciplines, including many geoscience subdisciplines, show a persistent gender gap. PROmoting Geo- science Research, Education, and SuccesS (PROGRESS) is a theory-driven role modeling and mentoring program aimed at supporting undergraduate women interested in geoscience-related degree and career pathways. This study is unique because it is being conducted in a long-term applied setting, rather than as a laboratory exercise. We compare female STEM majors in PROGRESS to a matched control group (N = 380) using a longitudinal prospec- tive multisite quasi-experimental design. College women in PROGRESS par- ticipated in a mentoring and role-modeling weekend workshop with follow- up support, while women in the control group participated in neither the workshop nor the follow-up support. PROGRESS members identified more female STEM career role models than controls (60% versus 42%, respectively), suggesting that deliberate interventions can develop the networks of under- graduate women. Undergraduate women that participate in PROGRESS have higher rates of persistence in geoscience-related majors (95% versus 73%), although the rates of switching into a geoscience-related major did not differ across groups. More strikingly, we also find that the persistence of undergrad- uate women in geoscience-related majors is related to the number of female STEM career role models they identify, as their odds of persisting approxi- mately doubles for each role model they identify. We conclude that our ability to retain undergraduate women in the geosciences will depend, in part, on helping them to identify same-gender career role models. Further, the suc- cess of PROGRESS points to steps universities and departments can take to sustain their students’ interest and persistence, such as hosting interactive panels with diverse female scientists to promote the attainability and social relevance of geoscience careers

Combined mTOR and MEK inhibition is an effective therapy in a novel mouse model for angiosarcoma

Angiosarcoma is an aggressive malignancy of vascular origin that occurs de novo or in the context of previous cancer therapy. Despite multi-modal aggressive treatment including surgical resection, chemotherapy, and radiation, five-year overall survival remains poor at 35%. Due to its rarity, little is known about its molecular pathology and clinical trials have been extremely difficult to conduct. Development of animal models for rare diseases like angiosarcoma is critical to improve our understanding of tumorigenesis and to test novel treatment regimens. A genetically engineered mouse model for angiosarcoma was generated by conditional deletion of Trp53, Pten, and Ptpn12 in endothelial cells. Tumors arising from these mice recapitulate the histology and molecular pathology of the human disease including hyperactivation of the PI3K/mTOR and MAPK signaling pathways. Treatment of tumor-bearing mice with mTOR or MEK inhibitors effectively inactivated signaling and resulted in reduced proliferation and elevated apoptosis leading to tumor regression. The effect of treatment on tumor growth was transient and proliferation was restored after a period of dormancy. However, combined inhibition of mTOR and MEK resulted in profound tumor regression which was sustained for the duration of treatment. These results suggest that angiosarcoma may be effectively treated by this drug combination