Building the future with on demand 3D printing

The current shorts in single use (SU) supply chains show how dependent both industry and academia are from only a few vendors worldwide. This is severely hindering fundamental research and process development for the pandemic response. With 3D printing technology we can manufacture SU equipment on demand and on site. In this study we investigated different commercially available low-cost materials and their compatibility for cell culture. We identified poly lactic acid (PLA) as perfect candidate for 3D printed parts for cell culture applications. The worldwide supply chain issues for SU shaking flasks and reactors gave us the incentive to develop 3D printed counterparts to maintain our HEK293 cell culture. The shake flasks were designed in Autodesk inventor 3D CAD. The materials tested represent the market of different 3D printing technologies and materials, ranging from UV-polymerizing resin printers to thermoplastic printers. We included different manufacturers, plant derived and water washable resins as well as medical Class IIa resins. Whereas resin printed shaking flasks needed washing, curing and sterilization using isopropyl alcohol, the thermoplastic flasks were directly autoclaved. The different materials were tested with HEK293 cells under standard conditions. Cell growth and viability were monitored daily. Please click Additional Files below to see the full abstract

3D printing technology: Supply chain independent single-use plastic ware and bioreactors for cell culture

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High cell density culture for VLP production in latent virus-free insect cell line

Continuous virus-like particle (VLP) production in insect cells with the baculo virus expression system faces numerous problems: The presence of defective interfering particles (DIPs) hinders continuous infection, cell growth is slower than virus replication, cell retention membranes and hollow fibres retain VLPs. High cell densities (HCD) can nevertheless enable VLP process intensification in small reactor vessels. We were able to achieve a HCD with the Tnms42 insect cell line in both shaking flasks with pseudo perfusion and in the bioreactor utilizing an alternating tangential flow (ATF) filtration. Compared to some commercially available insect cell lines, this cell line has no persistent adventitious viral infection and is equally well suited to produce HA-GAG VLPs as HighFiveTM cells. With daily medium exchange the exponential growth phase could be elongated and the total cell concentration (TCC) increased by a factor of two in the shaking flask (SF) and four in the bioreactor (BR) to ~40x106 cells/mL in comparison to reference batch processes (~9 Figure 1). Such HCD can be used to optimize and investigate cell concentration at infection (CCI), multiplicity of infection (MOI) and cell state at infection to reach higher volumetric VLP titers. Please click Download on the upper right corner to see the full abstract

Ein Standard für die Berichterstattung von Social Entrepreneurs

Social Entrepreneurs oder Sozialunternehmer gehen gesellschaftliche Probleme an und zielen nicht auf die Maximierung des ökonomischen Gewinns. Um die Attraktivität solcher Unternehmungen für Investoren zu steigern, ist eine standardisierte Berichterstattung sinnvoll. Ein solcher Standard muss auf die Besonderheiten von Social Entrepreneurs abgestimmt sein

COSMOS-Europe : a European network of cosmic-ray neutron soil moisture sensors

We thank TERENO (Terrestrial Environmental Observatories), funded by the Helmholtz-Gemeinschaft for the financing and maintenance of CRNS stations. We acknowledge financial support by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) of the research unit FOR 2694 Cosmic Sense (grant no. 357874777) and by the German Federal Ministry of Education of the Research BioökonomieREVIER, Digitales Geosystem – Rheinisches Revier project (grant no. 031B0918A). COSMOS-UK has been supported financially by the UK’s Natural Environment Research Council (grant no. NE/R016429/1). The Olocau experimental watershed is partially supported by the Spanish Ministry of Science and Innovation through the research project TETISCHANGE (grant no. RTI2018-093717-BI00). The Calderona experimental site is partially supported by the Spanish Ministry of Science and Innovation through the research projects CEHYRFO-MED (grant no. CGL2017-86839- C3-2-R) and SILVADAPT.NET (grant no. RED2018-102719-T) and the LIFE project RESILIENT FORESTS (grant no. LIFE17 CCA/ES/000063). The University of Bristol’s Sheepdrove sites have been supported by the UK’s Natural Environment Research Council through a number of projects (grant nos. NE/M003086/1, NE/R004897/1, and NE/T005645/1) and by the International Atomic Energy Agency of the United Nations (grant no. CRP D12014). Acknowledgements. We thank Peter Strauss and Gerhab Rab from the Institute for Land and Water Management Research, Federal Agency for Water Management Austria, Petzenkirchen, Austria. We thank Trenton Franz from the School of Natural Resources, University of Nebraska–Lincoln, Lincoln, NE, United States. We also thank Carmen Zengerle, Mandy Kasner, Felix Pohl, and Solveig Landmark, UFZ Leipzig, for supporting field calibration, lab analysis, and data processing. We furthermore thank Daniel Dolfus, Marius Schmidt, Ansgar Weuthen, and Bernd Schilling, Forschungszentrum Jülich, Germany. The COSMOS-UK project team is thanked for making its data available to COSMOS-Europe. Luca Stevanato is thanked for the technical details about the Finapp sensor. The stations at Cunnersdorf, Lindenberg, and Harzgerode have been supported by Falk Böttcher, Frank Beyrich, and Petra Fude, German Weather Service (DWD). The Zerbst site has been supported by Getec Green Energy GmbH and Jörg Kachelmann (Meteologix AG). The CESBIO sites have been supported by the CNES TOSCA program. The ERA5-Land data are provided by ECMWF (Muñoz Sabater, 2021). The Jena dataset was retrieved at the site of The Jena Experiment, operated by DFG research unit FOR 1451.Peer reviewedPublisher PD

Quantitative Forschungsarbeit über die Zusammenhänge von Arbeitsengagement, Burnout und allgemeiner Lebenszufriedenheit

Lena Achleitner, B. Sc.Zusammenfassung in englischer SpracheAlpen Adria Universität Klagenfurt, Masterarbeit, 2016(VLID)241469