Determining Signalling Nodes for Apoptosis by a Genetic High-Throughput Screen

With the ever-increasing information emerging from the various sequencing and gene annotation projects, there is an urgent need to elucidate the cellular functions of the newly discovered genes. The genetically regulated cell suicide of apoptosis is especially suitable for such endeavours as it is governed by a vast number of factors.We have set up a high-throughput screen in 96-well microtiter plates for genes that induce apoptosis upon their individual transfection into human cells. Upon screening approximately 100,000 cDNA clones we determined 74 genes that initiate this cellular suicide programme. A thorough bioinformatics analysis of these genes revealed that 91% are novel apoptosis regulators. Careful sequence analysis and functional annotation showed that the apoptosis factors exhibit a distinct functional distribution that distinguishes the cell death process from other signalling pathways. While only a minority of classic signal transducers were determined, a substantial number of the genes fall into the transporter- and enzyme-category. The apoptosis factors are distributed throughout all cellular organelles and many signalling circuits, but one distinct signalling pathway connects at least some of the isolated genes. Comparisons with microarray data suggest that several genes are dysregulated in specific types of cancers and degenerative diseases.Many unknown genes for cell death were revealed through our screen, supporting the enormous complexity of cell death regulation. Our results will serve as a repository for other researchers working with genomics data related to apoptosis or for those seeking to reveal novel signalling pathways for cell suicide

The societal context of professional practice: Examining the impact of politics and economics on journalistic role performance across 37 countries

The impact of socio-political variables on journalism is an ongoing concern of comparative research on media systems and professional cultures. However, they have rarely been studied systematically across diverse cases, particularly outside Western democracies, and existing studies that compare western and non-western contexts have mainly focused on journalistic role conceptions rather than actual journalistic practice. Using journalistic role performance as a theoretical and methodological framework, this paper overcomes these shortcomings through a content analysis of 148,474 news stories from 365 print, online, TV, and radio outlets in 37 countries. We consider two fundamental system-level variables—liberal democracy and market orientation—testing a series of hypotheses concerning their influence on the interventionist, watchdog, loyal-facilitator, service, infotainment, and civic roles in the news globally. Findings confirm the widely asserted hypothesis that liberal democracy is associated with the performance of public-service oriented roles. Claims that market orientation reinforces critical and civic-oriented journalism show more mixed results and give some support to the argument that there are forms of “market authoritarianism” associated with loyalist journalism. The findings also show that the interventionist and infotainment roles are not significantly associated with the standard measures of political and economic structure, suggesting the need for more research on their varying forms across societies and the kinds of system-level factors that might explain them

Journalistic Role Performance in Times of COVID

This paper examines journalistic role performance in coverage of the COVID-19 pandemic, based on a content analysis of newspaper, television, radio and online news in 37 countries. We test a set of hypotheses derived from two perspectives on the role of journalism in health crises. Mediatization theories assume that news media tend to sensationalize or to politicize health crises. A contrasting perspective holds that journalists shift toward more deferential and cooperative stances toward health and political authorities in a health crisis, attempting to mobilize the public to act according to the best science. Hypotheses derived from these perspectives are tested using the standard measures of journalistic roles developed by the Journalistic Role Performance Project. Results show that the deference/cooperation/consensus perspective is better supported, with media moving away from the Watchdog and Infotainment, and toward performance of the Service and Civic roles. We also explore differences in the pattern by country

Multisystem Inflammatory Syndrome in Children: Survey of Protocols for Early Hospital Evaluation and Management.

ObjectiveTo describe the similarities and differences in the evaluation and treatment of multisystem inflammatory syndrome in children (MIS-C) at hospitals in the US.Study designWe conducted a cross-sectional survey from June 16 to July 16, 2020, of US children's hospitals regarding protocols for management of patients with MIS-C. Elements included characteristics of the hospital, clinical definition of MIS-C, evaluation, treatment, and follow-up. We summarized key findings and compared results from centers in which >5 patients had been treated vs those in which ≤5 patients had been treated.ResultsIn all, 40 centers of varying size and experience with MIS-C participated in this protocol survey. Overall, 21 of 40 centers required only 1 day of fever for MIS-C to be considered. In the evaluation of patients, there was often a tiered approach. Intravenous immunoglobulin was the most widely recommended medication to treat MIS-C (98% of centers). Corticosteroids were listed in 93% of protocols primarily for moderate or severe cases. Aspirin was commonly recommended for mild cases, whereas heparin or low molecular weight heparin were to be used primarily in severe cases. In severe cases, anakinra and vasopressors frequently were recommended; 39 of 40 centers recommended follow-up with cardiology. There were similar findings between centers in which >5 patients vs ≤5 patients had been managed. Supplemental materials containing hospital protocols are provided.ConclusionsThere are many similarities yet key differences between hospital protocols for MIS-C. These findings can help healthcare providers learn from others regarding options for managing MIS-C