Cold atmospheric plasma, a novel approach against bladder cancer, with higher sensitivity for the high-grade cell line

Antitumor therapies based on Cold Atmospheric Plasma (CAP) are an emerging medical field. In this work, we evaluated CAP effects on bladder cancer. Two bladder cancer cell lines were used, HT-1376 (stage III) and TCCSUP (stage IV). Cell proliferation assays were performed evaluating metabolic activity (MTT assay) and protein content (SRB assay). Cell viability, cell cycle, and mitochondrial membrane potential (Δψm) were assessed using flow cytometry. Reactive oxygen and nitrogen species (RONS) and reduced glutathione (GSH) were evaluated by fluorescence. The assays were carried out with different CAP exposure times. For both cell lines, we obtained a significant reduction in metabolic activity and protein content. There was a decrease in cell viability, as well as a cell cycle arrest in S phase. The Δψm was significantly reduced. There was an increase in superoxide and nitric oxide and a decrease in peroxide contents, while GSH content did not change. These results were dependent on the exposure time, with small differences for both cell lines, but overall, they were more pronounced in the TCCSUP cell line. CAP showed to have a promising antitumor effect on bladder cancer, with higher sensitivity for the high-grade cell line.info:eu-repo/semantics/publishedVersio

Open-Air Cold Plasma Device Leads to Selective Tumor Cell Cytotoxicity

The need for effective and safe therapies for cancer is growing as aging is modifying its epidemiology. Cold atmospheric plasma (CAP) has gained attention as a potential anti-tumor therapy. CAP is a gas with enough energy to ionize a significant fraction of its constituent particles, forming equal numbers of positive ions and electrons. Timely-resolved output voltage measurement, emission spectroscopy, and quantification of reactive species (RS) in plasma-activated media (PAM) were performed to characterize the physical and chemical properties of plasma. To assess the cytotoxicity of cold atmospheric plasma in human tumors, different cell lines were cultured, plated, and exposed to CAP, followed by MTT and SRB colorimetric assays 24 h later. Human fibroblasts, phenotypically normal cells, were processed similarly. Plasma cytotoxicity was higher in cells of breast cancer, urinary bladder cancer, osteosarcoma, lung cancer, melanoma, and endometrial cancer. Cytotoxicity was time-dependent and possibly related to the increased production of hydrogen peroxide in the exposed medium. Sixty seconds of CAP exposure renders selective effects, preserving the viability of fibroblast cells. These results point to the importance of conducting further studies of the therapy with plasma

Saneamento e saúde: casos arboviroses associado a falta de tratamento de esgoto na cidade de Serra Grande-PB

Atualmente cerca de 5,3 bilhões pessoas vivem em área de restrição hídrica por, pelo menos, um mês ao ano e por volta de 500 milhões de pessoas estão em áreas onde o consumo de água excedente está diretamente ligado à qualidade da água já que contaminação das fontes pode impedir diferentes tipos de uso. 24 da mesma Resolução, dispõem sobre as condições e padrões de lançamento de efluentes em corpos hídricos, o qual estabelece que qualquer fonte poluidora só poderá ser lançada em corpos de água, seja de forma direta ou indireta, após tratamento devido, o qual dever seguir padrões e condições dispostas nessa Resolução. Umas das proposta para a melhoria no fornecimento de serviço de saneamento básico surge a partir do Estudo de Regionalização da prestação de serviços de saneamento o básico de água e esgotamento sanitário na Paraíba são projetados para atender a lei federal 14.026/2020, permitindo que os países promulguem por meio de leis complementares microrregiões de Saneamento Básico para fornecer Saúde, cuja finalidade é organizar, planejar, executar e operado em conjunto e integrado pelo Estado e os municípios que os compõem . Principal objetivo da Lei mencionada é garantir o acesso a propagação dos serviços de água e esgoto no território nacional, considerando os índices elevados na ausência desses serviços que causa negatividade em setores importante como saúde pública, meio ambiente, educação, econômico e social, sendo assim, um instrumento que efetiva fronteiras e limites com intuito de expandir a eficiência e o impacto das políticas públicas. Dessa forma este trabalho teve como objetivo levantar questões acerca da política Municipal sobre os serviços de saneamento básico na cidade de Serra Grande-PB e os casos de dengue que a população foi acometida. É importante ressaltar que a cidade em questão é um considerado município e pequeno porte com uma população de quase 3 mil habitantes. Para metodologia Em geral, a abordagem adotada para atingir os objetivos da pesquisa caracteriza-se pelo uso de métodos descritivos, pesquisa de campo e revisão bibliográfica em artigos científicos, livros, dissertações, anais de congressos, publicações do Ministério da Saúde e do MCidades, dados do SNIS, sites da Internet e legislações pertinentes. Como resultados pode ser constatado neste estudo, que a cidade de Serra Grande carece de estruturas de saneamento básico e de serviços de coleta, transporte e tratamento de esgoto visando prevenir a propagação de doenças, proteger o meio ambiente e garantir a qualidade de vida

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Relationship between the sprint and vertical jumps' power in young soccer players

Muscle power, speed and acceleration are generally considered important determinants of high performance in sports. Thus, the aim of this study was to evaluate the relationships between 10- and 30-meter sprint power and vertical jump power among young soccer players. The participants of the study were 143 male soccer players from the categories Under-20 (n = 34), Under-17 (n = 43) and Under-15 (n = 66), which performed vertical jump test, 10-meter sprint test and 30-meter sprint test. The results indicated weak to moderate correlations when the absolute values of time and maximal height were compared (r = −.47 to .71). When the values of power obtained from tests were compared, moderate to strong correlations were found (r = .74 to .87). The results suggest that implementing these three tests would be important because they measure different motor skills. Moreover, the correlation between vertical jump power and 30-meter sprint power was surprisingly strong. Nevertheless, further studies are required in order to improve understanding about these power relationships

Abstracts of the International Congress of Research Center in Sports Sciences, Health Sciences & Human Development (2016)

The papers published in this book of abstracts / proceedings were submitted to the Scientific Commission of the International Congress of Research Center in Sports Sciences, Health Sciences & Human Development, held on 11 and 12 November 2016, at the University of Évora, Évora, Portugal, under the topic of Exercise and Health, Sports and Human Development. The content of the abstracts is solely and exclusively of its authors responsibility. The editors and the Scientific Committee of the International Congress of Research Center in Sports Sciences, Health Sciences & Human Development do not assume any responsibility for the opinions and statements expressed by the authors. Partial reproduction of the texts and their use without commercial purposes is allowed, provided the source / reference is duly mentioned