A Utilização do Lúdico no Ensino de Química Orgânica: Uma Prática Envolvendo Jogos

Anais do 3° Encontro Nacional de Jogos e Atividades Lúdicas no Ensino de Química, Física e Biologia (Jalequim - Level III) - Núcleo de Desenvolvimento de Pesquisas em Ensino de Química/Ciências da UNILA (NuDDEQ)Um dos desafios dos professores de Química do terceiro ano do Ensino Médio está em tornar o estudo das funções orgânicas mais atrativo e interessante aos alunos. Uma das dificuldades encontradas é a de quebrar o caráter repetitivo que a abordagem desse conteúdo pode conter. Dentro desse contexto, a utilização de atividades lúdicas pode contribuir para o processo de aprendizagem dos alunos e melhorar a participação dos estudantes em sala de aula. Nesse panorama, utilizou-se o aplicativo Quizlet, disponível para celulares e computadores, que se baseia na utilização de flashcards e jogos. Através do aplicativo, buscou-se trabalhar com os alunos a diferenciação de funções e grupos funcionais

Condutibilidade hidráulica do solo saturado sob mata e pastagem em Uruará Transamazônica, Pará.

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The signalling effect of eco-labels in modern coastal tourism

As the demand for environmentally sustainable tourism grows, eco-labels are becoming increasingly popular as a signal of environmental quality. However, the existence of a causal link between awarding a seaside eco-label and the increase in tourism flows is still under discussion in the literature. In this article, we gauge the signalling impact of a specific eco-label, the Blue Flag award, using detailed data on tourism flows to seaside Italian destinations during the period 2008-2012. We adopt a recent econometric modelling strategy - the synthetic control method - in shaping estimation results and testing the sensitivity and robustness of our results. We find that being awarded the Blue Flag increases the flow of domestic tourists for up to three seasons after assignment. However, we find no effect for the flow of international tourists. Investigating the mechanisms driving the results, we find that the award of a Blue Flag only positively affects the flow of domestic tourists when it is used as a driver of organisation, coordination and integrated management of the tourism supply

Improving the downstream processing of interferon alfa-2b using alternative purification platforms based on ionic liquids

Improvements on human life expectancy and the lack of effective therapies has led to an increment of chronic diseases, being the application of biopharmaceuticals an efficient strategy to mitigate this scenario. Among the current available biopharmaceuticals, the role of interferon α-2b (IFNα-2b) should be highlighted, as it has been marketed over 30 years with a considerable impact on the global therapeutic proteins market (Castro et al, Vaccines, 2021). IFN manufacturing requires the use of the recombinant DNA technology, involving two main stages, the upstream and downstream stages. The first includes recombinant protein production in a suitable host microorganism, such as Escherichia coli (Castro et al, Sep. Purif. Technol., 2020), while the second comprises protein recovery, isolation, purification and polishing. Due to the high demands of the pharmaceutical industry for products with high purity and biological activity, the downstream stage is responsible for the majority of the production costs of biopharmaceuticals (50–90%), often including time-consuming and multi-step processes. Therefore, there is an immediate need to develop more efficient, cost-effective, and sustainable protein purification methodologies. In this work, two ionic-liquid-(IL)-based strategies were investigated for the purification of IFNα-2b recombinantly produced from E. coli fermentation broth, namely as adjuvants in aqueous biphasic systems or as chromatographic ligands immobilized in solid materials. Overall, the obtained results demonstrate that by tailoring IL’s chemical structures, improved protein purification processes are obtained and that the secondary structure of proteins is preserved.publishe

Sustainable ionic-liquid-based strategies for the downstream processing of interferon α-2b from Echerichia coli

Over the last decades, society has been facing an increment of chronic diseases due to the higher human life expectancy and the lack of efficient treatments for several pathologies. In this regard, biopharmaceuticals have become one of the most effective clinical treatments for a broad range of diseases, including cancer, metabolic and neurodegenerative disorders [1]. Among biopharmaceuticals, the role of interferons, particularly interferon α-2b (IFNα-2b), should be underlined, as they have been marketed for over 30 years with a considerable impact on the global therapeutic proteins market [2]. Usually based on the recombinant DNA technology, the manufacturing process of biopharmaceuticals encompasses two main stages: the upstream and downstream stages. Typically, the upstream phase includes recombinant protein production processes in a suitable host microorganism, such as Escherichia coli [3], while the general downstream processing of biopharmaceuticals comprises four stages - recovery, isolation, purification and polishing -, which are responsible for the majority of the production costs of biopharmaceuticals (50–90%) [3]. The downstream processing is a time-consuming and multi-step process, for which the development of cost-effective purification processes is mandatory to decrease their costs and environmental impact. In this context, two ionic-liquid-(IL)-based strategies were investigated in this work for the purification of IFNα-2b recombinantly produced from E. coli fermentation broth. ILs have been used as adjuvants in aqueous two-phase systems (ATPS) and applied in supported materials as alternative ligands. The obtained results demonstrate that ILs have a tailoring ability and contribute to the development of more effective and sustainable downstream processes of biopharmaceuticals.publishe

Application of the Quick Tool Exchange (QTE) System in Building Maintenance

Quick Tool Exchange (QTE) acts to reduce losses and failures during the production process and throughput time, contributing to rapid response to market changes. The object of the work is a private school of the Municipality of Porto Velho, capital of Rondônia. The general objective is to study the building maintenance of a private school in the Municipality of Porto Velho / RO, focusing on QTE technology. Therefore, the specific objectives were to characterize the building maintenance in the study (1); compare the operational processes applied in the face of QTE concepts (2); and propose innovation for the operational improvement of building maintenance in operation (3). The building maintenance has a clear importance, since its compliance contributes to the preservation of life and health of users of any construction. Both the preventive and the corrective have been receiving the importance due to it, especially the preventive one, since this is always cheaper in comparison to the corrective one, since besides the damages caused to the physical structure of the building, there can exist to the users present in the occurrence of the fact. The methodological procedures consisted in carrying out a bibliographic survey followed by a case study. The results demonstrated measures approved and not approved by the QTE, suggesting simple innovations, but with significant consequences to the improvement of service rendering, minimizing costs in the area of building maintenance. This study is of interest to businessmen in general, especially the public or private educational sector, and those involved with the area of Production Administration

An AAL collaborative system: the AAL4ALL and a mobile assistant case study

"15th IFIP WG 5.5 Working Conference on Virtual Enterprises, PRO-VE 2014, Amsterdam, The Netherlands, October 6-8, 2014"The areas of Ambient Assisted Living (AAL) and Intelligent Systems (IS) are in full development, but there are still some issues to be resolved. One issue is the myriad of user oriented solutions that are rarely built to interact or integrate with other systems available in the market. In this paper we present the AAL4ALL project and the UserAccess implementation, showing a novel approach towards virtual organizations, interoperability and certification. The aim of this project is to provide a collaborative network of services and devices that connect every user and product from other developers, building a heterogeneous ecosystem. Thus establishing an environment for collaborative care systems, which may be available to the users in from of safety services, comfort services and healthcare services.Project "AAL4ALL", co-financed by the European Community Fund FEDER, through COMPETE - Programa Operacional Factores de Competitividade (POFC). Foundation for Science and Technology (FCT), Lisbon, Portugal, through Project PEst-C/CTM/LA0025/2013 and the project PEst-OE/EEI/UI0752/2014. Project CAMCoF - Context-aware Multimodal Communication Framework fund-ed by ERDF -European Regional Development Fund through the COMPETE Pro-gramme (operational programme for competitiveness) and by National Funds through the FCT - Fundação para a Ciência e a Tecnologia (Portuguese Foundation for Science and Technology) within project FCOMP-01-0124-FEDER-028980

Estabelecimento de população da praga psilídeo-dos-citros, Diaphorina citri (hemiptera: psyllidae) e de seu parasitoide exótico importado Diaphorencyrtus aligarhensis (hymenoptera: encyrtidae) em gaiolas de criação em condições de quarentena.

A citricultura nacional está ameaçada por mais uma praga exótica, o psilídeo-dos?citros, Diaphorina citri (Hemiptera: Psyllidae), vetora da doença Huanglongbing (HLB) causada por bactérias de floema, Candidatus Liberibacter spp. Este trabalho objetivou a manutenção da população da praga D. citri para a produção posterior em larga escala do parasitoide exótico importado Diaphorencyrtus aligarhensis (Hymenoptera: Encyrtidae) no país para o controle biológico da praga. A metodologia de criação da praga e do parasitóide foi realizada em gaiolas de criação em mudas de murta (Murraya paniculata), e mantidas em salas de criação climatizadas. O resultado da produção de D. citri a partir de dezembro/2016 foi de 600 adultos para abril/2017 de 1648 indivíduos sob condições controladas de laboratório. A produção do parasitóide importado D. aligarhensis em gaiola de criação infestada por D. citri foi a partir de dezembro/2016 de 218 adultos para abril/2017 de 768 indivíduos, ou seja, a população aumentou se comparada à população inicial. Brazilian citriculture is being threatened by one more exotic pest, the citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae), vector of Huanglongbing disease (HLB) cause by the bacteria Candidatus Liberibacter spp. This work focused on the maintenance of the D. citri pest population for a later mass production of the imported exotic parasitoid Diaphorencytrus aligarhensis (Hymenoptera: Encyrtidae) in the country for the biological control of D. citri. The rearing methodology of the pest and of the parasitoid used rearing cages with plantlets of Murraya paniculata placed in acclimatized rearing rooms. The result of the production of D. citri, under laboratory controlled conditions for December 2016 was 600 adults and to april 2017 of 1648 individuals. The production of the imported parasitoid D. aligarhensis in infested cages for december 2016 was 218 adults, and for april of the same year was 768 individuals, i.e. population increased when compared to the initial population

Entanglement of two-mode Gaussian states: characterization and experimental production and manipulation

A powerful theoretical structure has emerged in recent years on the characterization and quantification of entanglement in continuous-variable systems. After reviewing this framework, we will illustrate it with an original set-up based on a type-II OPO with adjustable mode coupling. Experimental results allow a direct verification of many theoretical predictions and provide a sharp insight into the general properties of two-mode Gaussian states and entanglement resource manipulation