Are reintroductions an effective way of mitigating against plant extinctions? CEE review 07-008 (SR32)

Re-introductions are considered by some conservation practitioners to be a controversial management option for mitigating threatened plant declines. The use of translocations (including re-introductions) has been criticised for the lack of monitoring and central recording, inappropriateness of the action due to genetic considerations, a lack of knowledge of the demography of the donor populations and inadequate information on the habitat requirements of the species. Despite these arguably justified criticisms, re-introductions are growing in use as practitioners see no other option for meeting management plan targets. Re-introductions have been proposed as options for overcoming habitat loss, habitat fragmentation and reproductive isolation. An extension of this increasingly interventionist approach, often termed assisted colonisation, is being considered as a potential method for preventing extinctions due to climatic shifts too rapid to allow corresponding species‟ distribution changes. This review evaluates the effectiveness of re-introductions as a conservation tool by using the available evidence to determine in what context plant translocations have improved the status of threatened species

Activity of a nitric oxide-generating wound treatment system against wound pathogen biofilms

This work was supported by Edixomed Ltd

Longitudinal Analysis of the Gill microbiomes of Atlantic Salmon from four Scottish farms reveals dynamics in bacterial richness and seasonal trends in diversity.

Atlantic Salmon aquaculture in Scotland is a major industry being both Scotland, and the UK’s largest food export. Gill disease, in particular Complex Gill Disease, is a significant challenge of salmon production. It is increasingly understood that the microbiome can influence host health and immunity. Therefore, the objective of the study is to identify and characterise the gill microbiome from stocking to harvest from four sites in Scotland 2018-2020. At each site, mucosal gill swabs were collected fortnightly (sites A &amp; C) or monthly (sites B &amp; G) from eight fish in two pens (n=623 fish). Gill samples underwent 16S rRNA Illumina MiSeq amplicon library preparation and analysis to characterise changes in the gill mucosal communities. Complex Gill disease was identified in sampled fish from each site (A: 20%, B: 11%, C: 24%, G: 13%).At the four sites we showed species richness (alpha diversity) varied over time ranging from 68 ±SD31 to 777 ±SD152 (average 353 ±SD 158). Interestingly, 1100–1500 degree-days after seawater transfer, a distinct decline in species richness and evenness was observed at three of the four sties (A:410 SD± 134 to 276 SD±86 , B:264 SD±67 to 156 SD±71 , C:356 SD±130 to 228 SD±89). In terms of community composition, 1) while there were similarities between all four sites, the communities were statistically different (R = 0.067, P&lt;0.001) from each farm, indicating that sites contributes to differences seen in the microbiome. Within each farm, a seasonal pattern in the microbiome was seen, with community shifts through winter-spring-summer-autumn (A: R2 = 0.11, P&lt;0.001, B: R2 = 0.30, P&lt;0.001, C: R2 = 0.22, P&lt;0.001, G: R2 = 0.11, P&lt;0.001). Proteobacteria dominated the gills (average: 73.6%), with Bacteriodota (average: 18.2%) also highly abundant at all sites. Overall, we have shown changes in the bacterial communities over time and between sites indicating both seasonal and temporal changes in the gill microbiome. Understanding this will help us to better understand the role of the gill microbiome and its role in fish health. <br/

Longitudinal Analysis of the Gill microbiomes of Atlantic Salmon from four Scottish farms reveals dynamics in bacterial richness and seasonal trends in diversity.

Atlantic Salmon aquaculture in Scotland is a major industry being both Scotland, and the UK’s largest food export. Gill disease, in particular Complex Gill Disease, is a significant challenge of salmon production. It is increasingly understood that the microbiome can influence host health and immunity. Therefore, the objective of the study is to identify and characterise the gill microbiome from stocking to harvest from four sites in Scotland 2018-2020. At each site, mucosal gill swabs were collected fortnightly (sites A &amp; C) or monthly (sites B &amp; G) from eight fish in two pens (n=623 fish). Gill samples underwent 16S rRNA Illumina MiSeq amplicon library preparation and analysis to characterise changes in the gill mucosal communities. Complex Gill disease was identified in sampled fish from each site (A: 20%, B: 11%, C: 24%, G: 13%).At the four sites we showed species richness (alpha diversity) varied over time ranging from 68 ±SD31 to 777 ±SD152 (average 353 ±SD 158). Interestingly, 1100–1500 degree-days after seawater transfer, a distinct decline in species richness and evenness was observed at three of the four sties (A:410 SD± 134 to 276 SD±86 , B:264 SD±67 to 156 SD±71 , C:356 SD±130 to 228 SD±89). In terms of community composition, 1) while there were similarities between all four sites, the communities were statistically different (R = 0.067, P&lt;0.001) from each farm, indicating that sites contributes to differences seen in the microbiome. Within each farm, a seasonal pattern in the microbiome was seen, with community shifts through winter-spring-summer-autumn (A: R2 = 0.11, P&lt;0.001, B: R2 = 0.30, P&lt;0.001, C: R2 = 0.22, P&lt;0.001, G: R2 = 0.11, P&lt;0.001). Proteobacteria dominated the gills (average: 73.6%), with Bacteriodota (average: 18.2%) also highly abundant at all sites. Overall, we have shown changes in the bacterial communities over time and between sites indicating both seasonal and temporal changes in the gill microbiome. Understanding this will help us to better understand the role of the gill microbiome and its role in fish health. <br/

3D visualisation tool for improving the resilience to urban and coastal flooding in Torbay, UK

This is the final version of the article. Available from Elsevier via the DOI in this record.Torbay, located in South West England, UK, is one of the Case Studies on the EU-funded project EU-CIRCLE, which is aimed at enhancing resilience of Critical Infrastructures (CI) to natural hazards. The region includes three urban centres (Torquay, Paignton and Brixham) and hosts more than 3 million tourists every year that contribute over £450 million to local economy. However, flooding, including coastal, fluvial and pluvial, has been a major threat to the area with more than 15 major incidents occurring since 1999. Rising sea levels, combined with increasing rainfall intensity, linked to climate change, are expected to exacerbate the problem. Better adaptation strategies are needed to safeguard CIs and services while improving resilience to climate hazards. EU-CIRCLE partners are engaged in a review of the existing capacity of flood defenses and the drainage systems in Torbay. To enhance the risk communication with the stakeholders, we adopted a high performance flood model to analyse the flood risk to Cis under a wide range of scenarios. The results are integrated into an innovative 3D visualization tool, showing the progress over time of any flood scenario in the region, via a fully interactive interface allowing stakeholders to better understand flood impacts to CIs.The work presented in this paper was partially funded by the ongoing EC H2020 EU-CIRCLE (GA 653824) and the FP7 PEARL (Preparing for Extreme And Rare events in coastaL regions, GA 603663) projects

Perturbation solution for one-dimensional flow to a constant-pressure boundary in a stress-sensitive reservoir

Most analyses of fluid flow in porous media are conducted under the assumption that the permeability is constant. In some “stress-sensitive” rock formations, however, the variation of permeability with pore fluid pressure is sufficiently large that it needs to be accounted for in the analysis. Accounting for the variation of permeability with pore pressure renders the pressure diffusion equation nonlinear and not amenable to exact analytical solutions. In this paper, the regular perturbation approach is used to develop an approximate solution to the problem of flow to a linear constant-pressure boundary, in a formation whose permeability varies exponentially with pore pressure. The perturbation parameter αD is defined to be the natural logarithm of the ratio of the initial permeability to the permeability at the outflow boundary. The zeroth-order and first-order perturbation solutions are computed, from which the flux at the outflow boundary is found. An effective permeability is then determined such that, when inserted into the analytical solution for the mathematically linear problem, it yields a flux that is exact to at least first order in αD. When compared to numerical solutions of the problem, the result has 5% accuracy out to values of αD of about 2—a much larger range of accuracy than is usually achieved in similar problems. Finally, an explanation is given of why the change of variables proposed by Kikani and Pedrosa, which leads to highly accurate zeroth-order perturbation solutions in radial flow problems, does not yield an accurate result for one-dimensional flow

Decitabine impact on the endocytosis regulator RhoA, the folate carriers RFC1 and FOLR1, and the glucose transporter GLUT4 in human tumors.

BackgroundIn 31 solid tumor patients treated with the demethylating agent decitabine, we performed tumor biopsies before and after the first cycle of decitabine and used immunohistochemistry (IHC) to assess whether decitabine increased expression of various membrane transporters. Resistance to chemotherapy may arise due to promoter methylation/downregulation of expression of transporters required for drug uptake, and decitabine can reverse resistance in vitro. The endocytosis regulator RhoA, the folate carriers FOLR1 and RFC1, and the glucose transporter GLUT4 were assessed.ResultsPre-decitabine RhoA was higher in patients who had received their last therapy &gt;3&nbsp;months previously than in patients with more recent prior therapy (P = 0.02), and varied inversely with global DNA methylation as assessed by LINE1 methylation (r = -0.58, P = 0.006). Tumor RhoA scores increased with decitabine (P = 0.03), and RFC1 also increased in patients with pre-decitabine scores ≤150 (P = 0.004). Change in LINE1 methylation with decitabine did not correlate significantly with change in IHC scores for any transporter assessed. We also assessed methylation of the RFC1 gene (alias SLC19A1). SLC19A1 methylation correlated with tumor LINE1 methylation (r = 0.45, P = 0.02). There was a small (statistically insignificant) decrease in SLC19A1 methylation with decitabine, and there was a trend towards change in SLC19A1 methylation with decitabine correlating with change in LINE1 methylation (r = 0.47, P &lt;0.15). While SLC19A1 methylation did not correlate with RFC1 scores, there was a trend towards an inverse correlation between change in SLC19A1 methylation and change in RFC1 expression (r = -0.45, P = 0.19).ConclusionsIn conclusion, after decitabine administration, there was increased expression of some (but not other) transporters that may play a role in chemotherapy uptake. Larger patient numbers will be needed to define the extent to which this increased expression is associated with changes in DNA methylation

Case study of the cascading effects on critical infrastructure in Torbay coastal/pluvial flooding with climate change and 3D visualisation

This is the final version. Available on open access from IWA Publishing via the DOI in this record.Critical infrastructures (CIs) are commonly designed, built and maintained based on rigorous standards in order to withstand the climate and weather-related pressures. However, shifts in climate characteristics may result in increases of the magnitude and frequency of potential risks, or expose specific CI to new or increased risks not previously considered. As vital components of the normal functioning of modern societies, their resilience encompasses the operational elements, their structural integrity and the capacity to maximise business output under climate stressors. In this work, we apply an integrated and participatory methodological approach to assess the risk and enhance the resilience of interconnected CIs to urban flooding under climate change. The proposed methodology has been applied to an extended case study in Torbay to extend previous works (Gibson et al. 2018), which seeks to protect coastal communities from future through using the proposed methodology to justify future investment in coastal defences, as a part of the validation of EU-CIRCLE projects developed methodologies.Natural Environment Research Council (NERC)UK Royal Academy of EngineeringEuropean UnionEngineering and Physical Sciences Research Council (EPSRC