Antarctic sea ice elevation from satellite radar altimetry

In situ measurements of sea ice thickness from ship and upward-looking sonar are used to assess the potential for satellite radar altimetry to provide information on Antarctic sea ice thickness. A climatology of satellite ice elevation estimates is compared to an Antarctic sea ice thickness climatology made from the Antarctic Sea Ice Processes and Climate ( ASPeCt) data set. In addition monthly, regional, satellite ice elevation estimates are compared to ULS ice draft data. The results show reasonable spatial agreement between the satellite and in-situ data, and show regional signals of change in ice elevation in line with that which would be expected. The results show some promise for providing information on Antarctic ice thickness from radar altimetry missions such as CryoSat. However, further studies into snow and ice density and the radar penetration into the Antarctic snow cover are required

Laforin, a Dual Specificity Phosphatase Involved in Lafora Disease, Is Present Mainly as Monomeric Form with Full Phosphatase Activity

Lafora Disease (LD) is a fatal neurodegenerative epileptic disorder that presents as a neurological deterioration with the accumulation of insoluble, intracellular, hyperphosphorylated carbohydrates called Lafora bodies (LBs). LD is caused by mutations in either the gene encoding laforin or malin. Laforin contains a dual specificity phosphatase domain and a carbohydrate-binding module, and is a member of the recently described family of glucan phosphatases. In the current study, we investigated the functional and physiological relevance of laforin dimerization. We purified recombinant human laforin and subjected the monomer and dimer fractions to denaturing gel electrophoresis, mass spectrometry, phosphatase assays, protein-protein interaction assays, and glucan binding assays. Our results demonstrate that laforin prevalently exists as a monomer with a small dimer fraction both in vitro and in vivo. Of mechanistic importance, laforin monomer and dimer possess equal phosphatase activity, and they both associate with malin and bind glucans to a similar extent. However, we found differences between the two states' ability to interact simultaneously with malin and carbohydrates. Furthermore, we tested other members of the glucan phosphatase family. Cumulatively, our data suggest that laforin monomer is the dominant form of the protein and that it contains phosphatase activity

Counting whales in a challenging, changing environment

Funding: Marie Curie International Incoming Fellowship within the 7th European Community Framework Programme (proposal Nu 253407 (call reference: FP7- PEOPLE-2009-IIF).Estimating abundance of Antarctic minke whales is central to the International Whaling Commission's conservation and management work and understanding impacts of climate change on polar marine ecosystems. Detecting abundance trends is problematic, in part because minke whales are frequently sighted within Antarctic sea ice where navigational safety concerns prevent ships from surveying. Using icebreaker-supported helicopters, we conducted aerial surveys across a gradient of ice conditions to estimate minke whale density in the Weddell Sea. The surveys revealed substantial numbers of whales inside the sea ice. The Antarctic summer sea ice is undergoing rapid regional change in annual extent, distribution, and length of ice-covered season. These trends, along with substantial interannual variability in ice conditions, affect the proportion of whales available to be counted by traditional shipboard surveys. The strong association between whales and the dynamic, changing sea ice requires reexamination of the power to detect trends in whale abundance or predict ecosystem responses to climate change.Publisher PDFPeer reviewe

The great ice mystery

Changes in the extent and thickness of sea ice could alter ocean circulation and so disrupt the climate. Jon Copley considers one of the big unknowns in the global warming debate

Thick and deformed Antarctic sea ice mapped with autonomous underwater vehicles

Satellites have documented trends in Antarctic sea-ice extent and its variability for decades, but estimating sea-ice thickness in the Antarctic from remote sensing data remains challenging. In situ observations needed for validation of remote sensing data and sea-ice models are limited; most have been restricted to a few point measurements on selected ice floes, or to visual shipboard estimates. Here we present three-dimensional (3D) floe-scale maps of sea-ice draft for ten floes, compiled from two springtime expeditions by an autonomous underwater vehicle to the near-coastal regions of the Weddell, Bellingshausen, and Wilkes Land sectors of Antarctica. Mean drafts range from 1.4 to 5.5 m, with maxima up to 16 m. We also find that, on average, 76% of the ice volume is deformed ice. Our surveys indicate that the floes are much thicker and more deformed than reported by most drilling and ship-based measurements of Antarctic sea ice. We suggest that thick ice in the near-coastal and interior pack may be under-represented in existing in situ assessments of Antarctic sea ice and hence, on average, Antarctic sea ice may be thicker than previously thought

A role for the tyrosine kinase ACK1 in neurotrophin signaling and neuronal extension and branching.

Neurotrophins are involved in many crucial cellular functions, including neurite outgrowth, synapse formation, and plasticity. Although these events have long been known, the molecular determinants underlying neuritogenesis have not been fully characterized. Ack1 (activated Cdc42-associated tyrosine kinase) is a non-receptor tyrosine kinase that is highly expressed in the brain. Here, we demonstrate that Ack1 is a molecular constituent of neurotrophin signaling cascades in neurons and PC12 cells. We report that Ack1 interacts with Trk receptors and becomes tyrosine phosphorylated and its kinase activity is increased in response to neurotrophins. Moreover, our data indicate that Ack1 acts upstream of the Akt and MAPK pathways. We show that Ack1 overexpression induces neuritic outgrowth and promotes branching in neurotrophin-treated neuronal cells, whereas the expression of Ack1 dominant negatives or short-hairpin RNAs counteract neurotrophin-stimulated differentiation. Our results identify Ack1 as a novel regulator of neurotrophin-mediated events in primary neurons and in PC12 cells

Wingless secretion promotes and requires retromer-dependent cycling of Wntless

Wnt ligands are lipid-modified, secreted glycoproteins that control multiple steps during embryogenesis and adult-tissue homeostasis. Little is known about the mechanisms underlying Wnt secretion. Recently, Wntless (Wls/Evi/Srt) was identified as a conserved multi-pass transmembrane protein whose function seems to be dedicated to promoting the release of Wnts. Here, we describe Wls accumulation in the Golgi apparatus of Wnt/Wingless (Wg)-producing cells in Drosophila, and show that this localization is essential for Wg secretion. Moreover, Wls localization and levels critically depend on retromer, a conserved protein complex that mediates endosome-to-Golgi protein trafficking in yeast. In the absence of the retromer components Dvps35 or Dvps26, but in presence of Wg, Wls is degraded and Wg secretion impaired. Our results indicate that Wg, clathrin-mediated endocytosis and retromer sustain a Wls traffic loop from the Golgi to the plasma membrane and back to the Golgi, thereby enabling Wls to direct Wnt secretion