Mechanistic Insights into the Cholesterol-dependent Binding of Perfringolysin O-based Probes and Cell Membranes

Cholesterol distribution in the cell is maintained by both vesicular and non-vesicular sterol transport. Non-vesicular transport is mediated by the interaction of membrane-embedded cholesterol and water-soluble proteins. Small changes to the lipid composition of the membrane that do not change the total cholesterol content, can significantly affect how cholesterol interacts with other molecules at the surface of the membrane. The cholesterol-dependent cytolysin Perfringolysin O (PFO) constitutes a powerful tool to detect cholesterol in membranes, and the use of PFO-based probes has flourished in recent years. By using a non-lytic PFO derivative, we showed that the sensitivity of the probes for cholesterol can be tuned by modifications introduced directly in the membrane-interacting loops and/or by modifying residues away from the membrane-interacting domain. Through the use of these biosensors on live RAW 264.7 cells, we found that changes in the overall cholesterol content have a limited effect on the average cholesterol accessibility at the surface of the membrane. We showed that these exquisite biosensors report on changes in cholesterol reactivity at the membrane surface independently of the overall cholesterol content in the membrane

Active Sampling-based Binary Verification of Dynamical Systems

Nonlinear, adaptive, or otherwise complex control techniques are increasingly relied upon to ensure the safety of systems operating in uncertain environments. However, the nonlinearity of the resulting closed-loop system complicates verification that the system does in fact satisfy those requirements at all possible operating conditions. While analytical proof-based techniques and finite abstractions can be used to provably verify the closed-loop system's response at different operating conditions, they often produce conservative approximations due to restrictive assumptions and are difficult to construct in many applications. In contrast, popular statistical verification techniques relax the restrictions and instead rely upon simulations to construct statistical or probabilistic guarantees. This work presents a data-driven statistical verification procedure that instead constructs statistical learning models from simulated training data to separate the set of possible perturbations into "safe" and "unsafe" subsets. Binary evaluations of closed-loop system requirement satisfaction at various realizations of the uncertainties are obtained through temporal logic robustness metrics, which are then used to construct predictive models of requirement satisfaction over the full set of possible uncertainties. As the accuracy of these predictive statistical models is inherently coupled to the quality of the training data, an active learning algorithm selects additional sample points in order to maximize the expected change in the data-driven model and thus, indirectly, minimize the prediction error. Various case studies demonstrate the closed-loop verification procedure and highlight improvements in prediction error over both existing analytical and statistical verification techniques.Comment: 23 page

Multimodal imaging of a vascularized idiopathic epiretinal membrane

PURPOSE: To report a case of vascularized idiopathic epiretinal membrane including the multimodal imaging. METHODS: Findings on clinical examination, color fundus photography, spectral-domain optical coherence tomography, optical coherence tomography angiography, and fluorescein angiography. RESULTS: Sixty-three-year-old woman with a previous rhegmatogenous retinal detachment (RRD) who presented with an asymptomatic idiopathic neovascular complex below an epiretinal membrane. It was assessed with fluorescein angiography, OCT and OCT-A. The vascularized ERM was already present previously to the retinal detachment. CONCLUSION: Our case report describes an example of idiopathic epiretinal membrane complicated by neovascularization; we hypothesized hypertension could be one of the factors in this case, as it may lead to decreased retinal perfusion and upregulation in vascular endothelial growth factor (VEGF). Multimodal imaging can help in detecting neovascularized ERM

Microlensing in H1413+117 : disentangling line profile emission and absorption in a broad absorption line quasar

On the basis of 16 years of spectroscopic observations of the four components of the gravitationally lensed broad absorption line (BAL) quasar H1413+117, covering the ultraviolet to visible rest-frame spectral range, we analyze the spectral differences observed in the P Cygni-type line profiles and have used the microlensing effect to derive new clues to the BAL profile formation. We confirm that the spectral differences observed in component D can be attributed to a microlensing effect lasting at least a decade. We show that microlensing magnifies the continuum source in image D, leaving the emission line region essentially unaffected. We interpret the differences seen in the absorption profiles of component D as the result of an emission line superimposed onto a nearly black absorption profile. We also find that the continuum source and a part of the broad emission line region are likely de-magnified in component C, while components A and B are not affected by microlensing. We show that microlensing of the continuum source in component D has a chromatic dependence compatible with the thermal continuum emission of a standard Shakura-Sunyaev accretion disk. Using a simple decomposition method to separate the part of the line profiles affected by microlensing and coming from a compact region from the part unaffected by this effect and coming from a larger region, we disentangle the true absorption line profiles from the true emission line profiles. The extracted emission line profiles appear double-peaked, suggesting that the emission is occulted by a strong absorber, narrower in velocity than the full absorption profile, and emitting little by itself. We propose that the outflow around H1413+117 is constituted by a high-velocity polar flow and a denser, lower velocity disk seen nearly edge-on.Comment: Accepted for publication in Astronomy and Astrophysic

Online Human Activity Recognition using Low-Power Wearable Devices

Human activity recognition~(HAR) has attracted significant research interest due to its applications in health monitoring and patient rehabilitation. Recent research on HAR focuses on using smartphones due to their widespread use. However, this leads to inconvenient use, limited choice of sensors and inefficient use of resources, since smartphones are not designed for HAR. This paper presents the first HAR framework that can perform both online training and inference. The proposed framework starts with a novel technique that generates features using the fast Fourier and discrete wavelet transforms of a textile-based stretch sensor and accelerometer. Using these features, we design an artificial neural network classifier which is trained online using the policy gradient algorithm. Experiments on a low power IoT device (TI-CC2650 MCU) with nine users show 97.7% accuracy in identifying six activities and their transitions with less than 12.5 mW power consumption.Comment: This is in proceedings of ICCAD 2018. The datasets are available at https://github.com/gmbhat/human-activity-recognitio

Black Silicon with high density and high aspect ratio nanowhiskers

Physical properties of black Silicon (b-Si) formed on Si wafers by reactive ion etching in chlorine plasma are reported in an attempt to clarify the formation mechanism and the origin of the observed optical and electrical phenomena which are promising for a variety of applications. The b-Si consisting of high density and high aspect ratio sub-micron length whiskers or pillars with tip diameters of well under 3 nm exhibits strong photoluminescence (PL) both in visible and infrared, which are interpreted in conjunction with defects, confinement effects and near band-edge emission. Structural analysis indicate that the whiskers are all crystalline and encapsulated by a thin Si oxide layer. Infrared vibrational spectrum of Si-O-Si bondings in terms of transverse-optic (TO) and longitudinal-optic (LO) phonons indicates that disorder induced LO-TO optical mode coupling can be an effective tool in assessing structural quality of the b-Si. The same phonons are likely coupled to electrons in visible region PL transitions. Field emission properties of these nanoscopic features are demonstrated indicating the influence of the tip shape on the emission. Overall properties are discussed in terms of surface morphology of the nano whiskers

Quantum simulation of thermodynamics in an integrated quantum photonic processor

One of the core questions of quantum physics is how to reconcile the unitary evolution of quantum states, which is information-preserving and time-reversible, with evolution following the second law of thermodynamics, which, in general, is neither. The resolution to this paradox is to recognize that global unitary evolution of a multi-partite quantum state causes the state of local subsystems to evolve towards maximum-entropy states. In this work, we experimentally demonstrate this effect in linear quantum optics by simultaneously showing the convergence of local quantum states to a generalized Gibbs ensemble constituting a maximum-entropy state under precisely controlled conditions, while introducing an efficient certification method to demonstrate that the state retains global purity. Our quantum states are manipulated by a programmable integrated quantum photonic processor, which simulates arbitrary non-interacting Hamiltonians, demonstrating the universality of this phenomenon. Our results show the potential of photonic devices for quantum simulations involving non-Gaussian states

Sobre el estado actual de la observación de estrellas de referencia de hemisferio sur (SRS) y las estrellas brillantes (BS) con el círculo meridiano repsold del observatorio de Cerro Calan

Las observaciones del programa de estrellas de referencia del hemisferio sur (SRS) y estrellas brillantes (BS) se comenzaron en el círculo meridiano Repsoid del Observatorio de Cerro Calán a fines de 1962. Este trabajo esta siendo realizado por astrónomos chilenos y soviéticos. Previamente a las observaciones, se hizo una investigación del instrumento. Hasta el 22 de mayo del presente año se habían efectuado 20.276 observaciones. La estimación del error medio de una observación es de: α (RA) = 0.025 sec δ; δ (Dec) = 0.45". Los cálculos de las observaciones se estén realizando actualmente, parte de ellos mediante computadores electrónicos. Para las observaciones en declinación, las lecturas de los círculos se registran fotográficamente y estos registros fotográficos se medirán mediante una máquina electrónica de medir que actualmente se construye en Pulkovo. Los primeros resultados de las reducciones en A.R. de las series fundamentales, muestran que el sistema adoptado en el catálogo FK4 requiere una corrección en la zona de -40ᵒ a -90ᵒ que podría alcanzar hasta 0.05 s.Asociación Argentina de Astronomí

Sobre el estado actual de la observación de estrellas de referencia de hemisferio sur (SRS) y las estrellas brillantes (BS) con el círculo meridiano repsold del observatorio de Cerro Calan

Las observaciones del programa de estrellas de referencia del hemisferio sur (SRS) y estrellas brillantes (BS) se comenzaron en el círculo meridiano Repsoid del Observatorio de Cerro Calán a fines de 1962. Este trabajo esta siendo realizado por astrónomos chilenos y soviéticos. Previamente a las observaciones, se hizo una investigación del instrumento. Hasta el 22 de mayo del presente año se habían efectuado 20.276 observaciones. La estimación del error medio de una observación es de: α (RA) = 0.025 sec δ; δ (Dec) = 0.45". Los cálculos de las observaciones se estén realizando actualmente, parte de ellos mediante computadores electrónicos. Para las observaciones en declinación, las lecturas de los círculos se registran fotográficamente y estos registros fotográficos se medirán mediante una máquina electrónica de medir que actualmente se construye en Pulkovo. Los primeros resultados de las reducciones en A.R. de las series fundamentales, muestran que el sistema adoptado en el catálogo FK4 requiere una corrección en la zona de -40ᵒ a -90ᵒ que podría alcanzar hasta 0.05 s.Asociación Argentina de Astronomí

Control of the chirality and polarity of magnetic vortices in triangular nanodots

Magnetic vortex dynamics in lithographically prepared nanodots is currently a subject of intensive research, particularly after recent demonstration that the vortex polarity can be controlled by in-plane magnetic field. This has stimulated the proposals of non-volatile vortex magnetic random access memories. In this work, we demonstrate that triangular nanodots offer a real alternative where vortex chirality, in addition to polarity, can be controlled. In the static regime, we show that vortex chirality can be tailored by applying in-plane magnetic field, which is experimentally imaged by means of Variable-Field Magnetic Force Microscopy. In addition, the polarity can be also controlled by applying a suitable out-of-plane magnetic field component. The experiment and simulations show that to control the vortex polarity, the out-of-plane field component, in this particular case, should be higher than the in-plane nucleation field. Micromagnetic simulations in the dynamical regime show that the magnetic vortex polarity can be changed with short-duration magnetic field pulses, while longer pulses change the vortex chirality.Comment: 18 pages, 11 figure