5,422 research outputs found
Fluorescence-based approach to estimate the chlorophyll-a concentration of a phytoplankton bloom in Ardley Cove (Antarctica)
A phytoplankton bloom occurred in Ardley Cove, King George Island in January 2016, during which maximum chlorophyll-a reached 9.87 mg/m3. Records show that blooms have previously not occurred in this area prior to 2010 and the average chlorophyll-a concentration between 1991 and 2009 was less than 2 mg/m3. Given the lack of in situ measurements and the poor performance of satellite algorithms in the Southern Ocean and Antarctic waters, we validate and assess several chlorophyll-a algorithms and apply an improved baseline fluorescence approach to examine this bloom event. In situ water properties including in vivo fluorescence, water leaving radiance, and solar irradiance were collected to evaluate satellite algorithms and characterize chlorophyll-a concentration, as well as dominant phytoplankton groups. The results validated the nFLH fluorescence baseline approach, resulting in a good agreement at this high latitude, high chlorophyll-a region with correlation at 59.46%. The dominant phytoplankton group within the bloom was micro-phytoplankton, occupying 79.58% of the total phytoplankton community. Increasing sea ice coverage and sea ice concentration are likely responsible for increasing phytoplankton blooms in the recent decade. Given the profound influence of climate change on sea-ice and phytoplankton dynamics in the region, it is imperative to develop accurate methods of estimating the spatial distribution and concentrations of the increasing occurrence of bloom events
Resonance Raman scattering in bulk 2H-MX2 (M=Mo, W; X=S, Se) and monolayer MoS2
published_or_final_versio
Preferred growth direction of III-V nanowires on differently oriented Si substrates
One of the nanowire (NW) characteristics is its preferred elongation direction. Here, we investigated the impact of Si substrate crystal orientation on the growth direction of GaAs NWs. We first studied the self-catalyzed GaAs NW growth on Si (111) and Si (001) substrates. SEM observations show GaAs NWs on Si (001) are grown along four directions without preference on one or some of them. This non-preferential NW growth on Si (001) is morphologically in contrast to the extensively reported vertical preferred GaAs NW growth on Si (111) substrates. We propose a model based on the initial condition of an ideal Ga droplet formation on Si substrates and the surface free energy calculation which takes into account the dangling bond surface density for different facets. This model provides further understanding of the different preferences in the growth of GaAs NWs along selected directions depending on the Si substrate orientation. To verify the prevalence of the model, NWs were grown on Si (311) substrates. The results are in good agreement with the three-dimensional mapping of surface free energy by our model. This general model can also be applied to predictions of NW preferred growth directions by the vapor-liquid-solid growth mode on other group IV and III–V substrates
Residual stress measurement in thin films using the semi-destructive ring-core drilling method using Focused Ion Beam
In the present study, residual stress evaluation in thin films was achieved using a semi-destructive trench-cutting method. Focused Ion Beam (FIB) was employed to introduce the strain relief by ring-core milling, i.e. creating a trench around an "island". Either SEM or FIB imaging can be used to record sequences of images for strain change evaluation by Digital Image Correlation (DIC) analysis of micrographs. A regular array of shallow holes was drilled on a thin overlayer of Pt (∼100nm) deposited on to the film prior to patterning and trenching, in order to reduce the damage introduced by the ion beam during imaging and to assist the DIC strain evaluation by adding traceable markers. Finite Element (FE) simulation was also carried out to predict the curves for strain relief as a function of milling depth, and compared with the experimental measurements, which show good agreement with each other. An empirical mathematical description of the curves was proposed that allows efficient residual stress evaluation in thin solid films. © 2011 Published by Elsevier Ltd
Lysosomal acidification dysfunction in microglia: an emerging pathogenic mechanism of neuroinflammation and neurodegeneration
Microglia are the resident innate immune cells in the brain with a major role in orchestrating immune responses. They also provide a frontline of host defense in the central nervous system (CNS) through their active phagocytic capability. Being a professional phagocyte, microglia participate in phagocytic and autophagic clearance of cellular waste and debris as well as toxic protein aggregates, which relies on optimal lysosomal acidification and function. Defective microglial lysosomal acidification leads to impaired phagocytic and autophagic functions which result in the perpetuation of neuroinflammation and progression of neurodegeneration. Reacidification of impaired lysosomes in microglia has been shown to reverse neurodegenerative pathology in Alzheimer's disease. In this review, we summarize key factors and mechanisms contributing to lysosomal acidification impairment and the associated phagocytic and autophagic dysfunction in microglia, and how these defects contribute to neuroinflammation and neurodegeneration. We further discuss techniques to monitor lysosomal pH and therapeutic agents that can reacidify impaired lysosomes in microglia under disease conditions. Finally, we propose future directions to investigate the role of microglial lysosomal acidification in lysosome-mitochondria crosstalk and in neuron-glia interaction for more comprehensive understanding of its broader CNS physiological and pathological implications
Making Gestural Interaction Accessible to Visually Impaired People
International audienceAs touch screens become widely spread, making them more accessible to visually impaired people is an important task. Touch displays possess a poor accessibility for visually impaired people. One possibility to make them more accessible without sight is through gestural interaction. Yet, there are still few studies on using gestural interaction for visually impaired people. In this paper we present a comprehensive summary of existing projects investigating accessible gestural interaction. We also highlight the limits of current approaches and propose future working directions. Then, we present the design of an interactive map prototype that includes both a raised-line map overlay and gestural interaction for accessing different types of information (e.g., opening hours, distances). Preliminary results of our project show that basic gestural interaction techniques can be successfully used in interactive maps for visually impaired people
Circular Single-Stranded Synthetic DNA Delivery Vectors for MicroRNA
Single-stranded (ss) circular oligodeoxynucleotides were previously found to undergo rolling circle transcription (RCT) by phage and bacterial RNA polymerases (RNAPs) into tandemly repetitive RNA multimers. Here, we redesign them to encode minimal primary miRNA mimics, with the long term aim of intracellular transcription followed by RNA processing and maturation via endogenous pathways. We describe an improved method for circularizing ss synthetic DNA for RCT by using a recently described thermostable RNA ligase, which does not require a splint oligonucleotide to juxtapose the ligating ends. In vitro transcription of four templates demonstrates that the secondary structure inherent in miRNA-encoding vectors does not impair their RCT by RNAPs previously shown to carry out RCT. A typical primary-miRNA rolling circle transcript was accurately processed by a human Drosha immunoprecipitate, indicating that if human RNAPs prove to be capable of RCT, the resulting transcripts should enter the endogenous miRNA processing pathway in human cells. Circular oligonucleotides are therefore candidate vectors for small RNA delivery in human cells, which express RNAPs related to those tested here
Hot Streaks in Artistic, Cultural, and Scientific Careers
The hot streak, loosely defined as winning begets more winnings, highlights a
specific period during which an individual's performance is substantially
higher than her typical performance. While widely debated in sports, gambling,
and financial markets over the past several decades, little is known if hot
streaks apply to individual careers. Here, building on rich literature on
lifecycle of creativity, we collected large-scale career histories of
individual artists, movie directors and scientists, tracing the artworks,
movies, and scientific publications they produced. We find that, across all
three domains, hit works within a career show a high degree of temporal
regularity, each career being characterized by bursts of high-impact works
occurring in sequence. We demonstrate that these observations can be explained
by a simple hot-streak model we developed, allowing us to probe quantitatively
the hot streak phenomenon governing individual careers, which we find to be
remarkably universal across diverse domains we analyzed: The hot streaks are
ubiquitous yet unique across different careers. While the vast majority of
individuals have at least one hot streak, hot streaks are most likely to occur
only once. The hot streak emerges randomly within an individual's sequence of
works, is temporally localized, and is unassociated with any detectable change
in productivity. We show that, since works produced during hot streaks garner
significantly more impact, the uncovered hot streaks fundamentally drives the
collective impact of an individual, ignoring which leads us to systematically
over- or under-estimate the future impact of a career. These results not only
deepen our quantitative understanding of patterns governing individual
ingenuity and success, they may also have implications for decisions and
policies involving predicting and nurturing individuals with lasting impact
Direct observation of spin-polarised bulk bands in an inversion-symmetric semiconductor
Methods to generate spin-polarised electronic states in non-magnetic solids
are strongly desired to enable all-electrical manipulation of electron spins
for new quantum devices. This is generally accepted to require breaking global
structural inversion symmetry. In contrast, here we present direct evidence
from spin- and angle-resolved photoemission spectroscopy for a strong spin
polarisation of bulk states in the centrosymmetric transition-metal
dichalcogenide WSe. We show how this arises due to a lack of inversion
symmetry in constituent structural units of the bulk crystal where the
electronic states are localised, leading to enormous spin splittings up to
eV, with a spin texture that is strongly modulated in both real and
momentum space. As well as providing the first experimental evidence for a
recently-predicted `hidden' spin polarisation in inversion-symmetric materials,
our study sheds new light on a putative spin-valley coupling in
transition-metal dichalcogenides, of key importance for using these compounds
in proposed valleytronic devices.Comment: 6 pages, 4 figure
Optoelectronics with electrically tunable PN diodes in a monolayer dichalcogenide
One of the most fundamental devices for electronics and optoelectronics is
the PN junction, which provides the functional element of diodes, bipolar
transistors, photodetectors, LEDs, and solar cells, among many other devices.
In conventional PN junctions, the adjacent p- and n-type regions of a
semiconductor are formed by chemical doping. Materials with ambipolar
conductance, however, allow for PN junctions to be configured and modified by
electrostatic gating. This electrical control enables a single device to have
multiple functionalities. Here we report ambipolar monolayer WSe2 devices in
which two local gates are used to define a PN junction exclusively within the
sheet of WSe2. With these electrically tunable PN junctions, we demonstrate
both PN and NP diodes with ideality factors better than 2. Under excitation
with light, the diodes show photodetection responsivity of 210 mA/W and
photovoltaic power generation with a peak external quantum efficiency of 0.2%,
promising numbers for a nearly transparent monolayer sheet in a lateral device
geometry. Finally, we demonstrate a light-emitting diode based on monolayer
WSe2. These devices provide a fundamental building block for ubiquitous,
ultra-thin, flexible, and nearly transparent optoelectronic and electronic
applications based on ambipolar dichalcogenide materials.Comment: 14 pages, 4 figure
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