Museu Virtual da Cidade Velha

Na actual sociedade da informação, a internet tem-se afirmando enquanto ferramenta de comunicação e fonte de informação, tornando importante que as mais variadas instituições marquem presença neste mundo digital online. Neste contexto, os museus têm vindo a adoptar estas ferramentas na sua relação com os públicos, sendo importante o conhecimento da realidade internacional. Assim, o presente trabalho de investigação teve por objectivos a determinação tanto dos conteúdos disponibilizados pelos museus virtuais como das ferramentas disponíveis para o desenvolvimento do Museu Virtual da Cidade Velha. Para tal, foi feita uma análise de alguns museus virtuais existentes que foram importantes para as tomadas de decisão durante o desenvolvimento do Museu da Cidade Velha. No que respeita aos conteúdos disponibilizados nos museus analisados podemos concluir que estes oferecem, sobretudo, informações de apresentação do museu, sobre colecções, exposições físicas e trabalhos de serviços educativos, sendo essa a forma de comunicação privilegiada em termos de internet. Realizou-se ainda, uma fundamentação teórica acerca dos principais conceitos relativos a museu virtual. Também foi feito um estudo sobre as tecnologias de implementação Web nomeadamente o Joomla e o DNN. Das conclusões tiradas com base nas análises, foi feito uma implementação de um protótipo para o Museu Virtual da Cidade Velha que poderá ser acessível através da Web.Universidade de Aveiro Ano 2010 Departamento de Electrónica, Telecomunicações e Informátic

Multi-walled carbon nanotubes as a platform for Immunoglobulin G attachment

Nanomaterials have been extensively used in different applications due to their peculiar characteristics and nanoscale dimensions. Among nanoparticles, carbon-based nanomaterials are becoming highly attractive for biomedical applications such as diagnosis, tissue engineering, drug delivery, and biosensing. The conjugation of carbon-based nanomaterials with antibodies combines the properties of these materials with the specific and selective recognition ability of the antibodies to antigens. The present work proposes a process intensification approach for immunoglobulin G (IgG present in rabbit serum) attachment on multi-walled carbon nanotubes (MWCNTs) in a single step. The effect of several parameters, namely MWCNTs external diameter, rabbit serum concentration, MWCNTs functionalization and pH value, on the IgG attachment yield was evaluated. The dilution of rabbit serum decreased other protein attachment, namely rabbit serum albumin (RSA), while increasing the IgG yield to 100%. The interaction mechanisms between IgG and MWCNTs were evaluated at pH 5.0 to 8.0. The protonation of IgG amino acids indicates that N-term are the most reactive amino acids in the antibody structure. The identification of the N-term reactivity at pH 8.0 allows to indicate a possible orientation of the antibody over the MWCNTs surface, described as “end-on”. Since the amount of RSA attached to MWNT decreased with the increase in serum dilution, the IgG orientation and amine activity was not affected. This orientation demonstrates that the IgG attachment over the surface of the MWCNTs could be an effective strategy to maintain the antigen recognition by the antibody, and to be used for biomedical applications.publishe

Phase diagrams of classical spin fluids: the influence of an external magnetic field on the liquid-gas transition

The influence of an external magnetic field on the liquid-gas phase transition in Ising, XY, and Heisenberg spin fluid models is studied using a modified mean field theory and Gibbs ensemble Monte Carlo simulations. It is demonstrated that the theory is able to reproduce quantitatively all characteristic features of the field dependence of the critical temperature T_c(H) for all the three models. These features include a monotonic decrease of T_c with rising H in the case of the Ising fluid as well as a more complicated nonmonotonic behavior for the XY and Heisenberg models. The nonmonotonicity consists in a decrease of T_c with increasing H at weak external fields, an increase of T_c with rising H in the strong field regime, and the existence of a minimum in T_c(H) at intermediate values of H. Analytical expressions for T_c(H) in the large field limit are presented as well. The magnetic para-ferro phase transition is also considered in simulations and described within the mean field theory.Comment: 14 pages, 12 figures (to be submitted to Phys. Rev. E

Comparing the safety and efficacy of ruxolitinib in patients with Dynamic International Prognostic Scoring System low-, intermediate-1-, intermediate-2-, and high-risk myelofibrosis in JUMP, a Phase 3b, expanded-access study

Ruxolitinib, a potent Janus kinase 1/2 inhibitor, has demonstrated durable improvements in patients with myelofibrosis. In this analysis of the Phase 3b JUMP study, which included patients aged =18 years with a diagnosis of primary or secondary myelofibrosis, we assessed the safety and efficacy of ruxolitinib in patients stratified by Dynamic International Prognostic Scoring System (DIPSS) risk categories. Baseline characteristic data were available to assess DIPSS status for 1844 of the 2233 enrolled patients; 60, 835, 755, and 194 in the low-, intermediate (Int)-1-, Int-2-, and high-risk groups, respectively. Ruxolitinib was generally well tolerated across all risk groups, with an adverse-event (AE) profile consistent with previous reports. The most common hematologic AEs were thrombocytopenia and anemia, with highest rates of Grade =3 events in high-risk patients. Approximately, 73% of patients experienced =50% reductions in palpable spleen length at any point in the =24-month treatment period, with highest rates in lower-risk categories (low, 82.1%; Int-1, 79.3%; Int-2, 67.1%; high risk, 61.6%). Median time to spleen length reduction was 5.1 weeks and was shortest in lower-risk patients. Across measures, 40%–57% of patients showed clinically meaningful symptom improvements, which were observed from 4 weeks after treatment initiation and maintained throughout the study. Overall survival (OS) was 92% at Week 72 and 75% at Week 240 (4.6 years). Median OS was longer for Int-2-risk than high-risk patients (253.6 vs. 147.3 weeks), but not evaluable in low-/Int-1-risk patients. By Week 240, progression-free survival (PFS) and leukemia-free survival (LFS) rates were higher in lower-risk patients (PFS: low, 90%; Int-1, 82%; Int-2, 46%; high risk, 15%; LFS: low, 92%; Int-1, 86%; Int-2, 58%; high risk, 19%). Clinical benefit was seen across risk groups, with more rapid improvements in lower risk patients. Overall, this analysis indicates that ruxolitinib benefits lower-risk DIPSS patients in addition to higher risk

Viability of dietary substitution of live microalgae with dry Ulva rigida in broodstock conditioning of the Pacific oyster (Crassostrea gigas)

The current study evaluated the microalgae replacement by dry macroalgae (Ulva rigida) in the reproductive success and biochemical composition of the Pacific oyster (Crassostrea gigas) during broodstock conditioning. Five nutritional regimes were tested: 100% macroalgae (diet 1), 50% macroalgae+50% microalgae (diet 2), 25% macroalgae+75% microalgae (diet 3) and 100% microalgae (diet 4). An unfed group was used as a negative control. The microalgae blend was composed of 33% Isochrysis galbana and 67% diatoms (75% Skeletonema costatum+25% Chaetoceros calcitrans). Gonadal maturation was reflected in the physiological condition of the individuals. All treatments, except diet 1, showed an increase in condition index and were fully matured at the end of the trial, with the best physiological condition observed in oysters fed diet 3 and diet 4. Protein and total lipid content increased during the conditioning period, whereas glycogen content decreased. Oysters conditioned with diet 3 had higher protein and total lipid content and lower glycogen content than the other treatments. In addition, diet 3 showed the highest percentage of viable veliger larvae. The current study demonstrated that it is possible to replace 25% of microalgae with macroalgae in the broodstock conditioning, minimizing the operative cost in bivalve hatcheries. © 2018. Published by The Company of BiologistsThis work was supported by Project INNOVMAR-Innovation and Sustainability in the Management and Exploitation of Marine Resources [NORTE-01-0145-FEDER-000035] within the line ‘INSEAFOOD, Innovation and valorization of seafood products’, funded by the Northern Regional Operational Programme (NORTE2020) through the European Regional Development Fund. Postdoctoral fellowships were granted to authors T.G.T. [SFRH/BPD/89360/2012] and to author A.C.G. [ref. SFRH/ BPD/72777/2010], by Fundaçaõ para a Ciência e a Tecnologia (Portugal), under the auspices of ESF and Portuguese funds. Z.E.M. is grateful to the project [UID/QUI/ 50006/2013-POCI/01/0145/FEDER/007265] with support from Fundaçaõ para a Ciência e a Tecnologia/MEC through national funds and co-financed by European Regional Development Fund, under the Partnership Agreement PT2020. This work was partially supported by POCI-01-0145-FEDER-006939 [Laboratory for Process Engineering, Environment, Biotechnology and Energy—UID/EQU/00511/2013], funded by the European Regional Development Fund, through COMPETE2020— Programa Operacional Competitividade e Internacionalizaçaõ and by national funds via Fundaçaõ para a Ciência e a Tecnologia, through project NORTE-01-0145-FEDER-000005—LEPABE-2-ECO-INNOVATION, supported by NORTE 2020, under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund

Feeding strategies and energy to protein ratio on tambaqui performance and physiology

The objective of this work was to evaluate the effect of feed deprivation and refeeding with diets containing different energy to protein ratios (E/P) on the performance and physiology of juvenile tambaqui (Colossoma macropomum). A 4x2 factorial arrangement with three replicates was used, with four E/P ratios (11.5, 10.5, 9.5, and 8.5 kcal g-1 digestible energy per protein) and two feeding regimens (with and without deprivation), during 60 days. Fish from the food-deprived group were fasted for 14 days and refed from the fifteenth to the sixtieth day, whereas the remaining fish were fed for 60 days. At the end of the experimental period, weight of fish subjected to food deprivation was lower than that of those continuously fed; however, this condition did not influence the physiological parameters analyzed. Tambaqui fed 11.5 kcal g-1 achieved lower final weight than those fed with the other diets, in both regimens. Among the physiological parameters, only plasma protein presented significant increase in fish fed 8.5 kcal g-1, in both feeding regimens, probably due to the higher dietary protein concentration. These results indicate that fish show a partial compensatory growth, and that 10.5 kcal g-1 can be recommended for the diet of juvenile tambaqui