340 research outputs found
Anonymous and EST-based microsatellite DNA markers that transfer broadly across the fig genus (Ficus, Moraceae)
• Premise of the study: We developed a set of microsatellite markers for broad utility across the species-rich pantropical tree genus Ficus (fig trees). The markers were developed to study population structure, hybridization, and gene flow in neotropical species.
• Methods and Results: We developed seven novel primer sets from expressed sequence tag (EST) libraries of F. citrifolia and F. popenoei (subgen. Urostigma sect. Americana) and optimized five previously developed anonymous loci for cross-species amplification. The markers were successfully tested on four species from the basal subgenus Pharmacosycea sect. Pharmaco- sycea (F. insipida, F. maxima, F. tonduzii, and F. yoponensis) and seven species of the derived subgenus Urostigma (F. citrifolia, F. colubrinae, F. costaricana, F. nymphaeifolia, F. obtusifolia, F. pertusa, and F. popenoei). The 12 markers amplified consis- tently and displayed polymorphism in all the species.
• Conclusions: This set of microsatellite markers is transferable across the phylogenetic breadth of Ficus, and should therefore be useful for studies of population structure and gene flow in approximately 750 fig species worldwide.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/92471/1/Heer2012.pdf8
Electric Field Controlled Magnetic Anisotropy in a Single Molecule
We have measured quantum transport through an individual Fe
single-molecule magnet embedded in a three-terminal device geometry. The
characteristic zero-field splittings of adjacent charge states and their
magnetic field evolution are observed in inelastic tunneling spectroscopy. We
demonstrate that the molecule retains its magnetic properties, and moreover,
that the magnetic anisotropy is significantly enhanced by reversible electron
addition / subtraction controlled with the gate voltage. Single-molecule
magnetism can thus be electrically controlled
Isolation and characterization of few-layer black phosphorus
Isolation and characterization of mechanically exfoliated black phosphorus
flakes with a thickness down to two single-layers is presented. A modification
of the mechanical exfoliation method, which provides higher yield of atomically
thin flakes than conventional mechanical exfoliation, has been developed. We
present general guidelines to determine the number of layers using optical
microscopy, Raman spectroscopy and transmission electron microscopy in a fast
and reliable way. Moreover, we demonstrate that the exfoliated flakes are
highly crystalline and that they are stable even in free-standing form through
Raman spectroscopy and transmission electron microscopy measurements. A strong
thickness dependence of the band structure is found by density functional
theory calculations. The exciton binding energy, within an effective mass
approximation, is also calculated for different number of layers. Our
computational results for the optical gap are consistent with preliminary
photoluminescence results on thin flakes. Finally, we study the environmental
stability of black phosphorus flakes finding that the flakes are very
hydrophilic and that long term exposure to air moisture etches black phosphorus
away. Nonetheless, we demonstrate that the aging of the flakes is slow enough
to allow fabrication of field-effect transistors with strong ambipolar
behavior. Density functional theory calculations also give us insight into the
water-induced changes of the structural and electronic properties of black
phosphorus.Comment: 11 main figures, 7 supporting figure
The Roots of Diversity: Below Ground Species Richness and Rooting Distributions in a Tropical Forest Revealed by DNA Barcodes and Inverse Modeling
F. Andrew Jones is with the Smithsonian Tropical Research Institute, David L. Erickson is with the Smithsonian Institution, Moises A. Bernal is with the Smithsonian Tropical Research Institute and UT Austin, Eldredge Bermingham is with the Smithsonian Tropical Research Institute, W. John Kress is with the Smithsonian Institution, Edward Allen Herre is with the Smithsonian Tropical Research Institute, Helene C. Muller-Landau is with the Smithsonian Tropical Research Institute, Benjamin L. Turner is with the Smithsonian Tropical Research Institute.Background -- Plants interact with each other, nutrients, and microbial communities in soils through extensive root networks. Understanding these below ground interactions has been difficult in natural systems, particularly those with high plant species diversity where morphological identification of fine roots is difficult. We combine DNA-based root identification with a DNA barcode database and above ground stem locations in a floristically diverse lowland tropical wet forest on Barro Colorado Island, Panama, where all trees and lianas >1 cm diameter have been mapped to investigate richness patterns below ground and model rooting distributions. Methodology/Principal Findings -- DNA barcode loci, particularly the cpDNA locus trnH-psba, can be used to identify fine and small coarse roots to species. We recovered 33 species of roots from 117 fragments sequenced from 12 soil cores. Despite limited sampling, we recovered a high proportion of the known species in the focal hectare, representing approximately 14% of the measured woody plant richness. This high value is emphasized by the fact that we would need to sample on average 13 m2 at the seedling layer and 45 m2 for woody plants >1 cm diameter to obtain the same number of species above ground. Results from inverse models parameterized with the locations and sizes of adults and the species identifications of roots and sampling locations indicates a high potential for distal underground interactions among plants. Conclusions -- DNA barcoding techniques coupled with modeling approaches should be broadly applicable to studying root distributions in any mapped vegetation plot. We discuss the implications of our results and outline how second-generation sequencing technology and environmental sampling can be combined to increase our understanding of how root distributions influence the potential for plant interactions in natural ecosystems.FAJ acknowledges the support of a Tupper postdoctoral fellowship in tropical biology and the National Science Foundation (DEB 0453665). Funding was provided by the Smithsonian Institution Global Earth Observatory, the Smithsonian Tropical Research Institute/Center for Tropical Forest Sciences endowment fund, and the Smithsonian Tropical Research Institute/Frank Levinson fund. We would like to thank Autoridad Nacional del Ambiente and the Smithsonian Tropical Research Institute for processing research permits. We thank S. Hubbell and R. Condit for access to plot data, S. Schnitzer for liana census data (NSF DEB 0613666), and L. Comita and S. Hubbell for access to seedling data (NSF DEB 0075102 and DEB 0823728). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Marine Scienc
Electron-hole symmetry in a semiconducting carbon nanotube quantum dot
Optical and electronic phenomena in solids arise from the behaviour of
electrons and holes (unoccupied states in a filled electron sea). Electron-hole
symmetry can often be invoked as a simplifying description, which states that
electrons with energy above the Fermi sea behave the same as holes below the
Fermi energy. In semiconductors, however, electron-hole symmetry is generally
absent since the energy band structure of the conduction band differs from the
valence band. Here we report on measurements of the discrete, quantized-energy
spectrum of electrons and holes in a semiconducting carbon nanotube. Through a
gate, an individual nanotube is filled controllably with a precise number of
either electrons or holes, starting from one. The discrete excitation spectrum
for a nanotube with N holes is strikingly similar to the corresponding spectrum
for N electrons. This observation of near perfect electron-hole symmetry
demonstrates for the first time that a semiconducting nanotube can be free of
charged impurities, even in the limit of few-electrons or holes. We furthermore
find an anomalously small Zeeman spin splitting and an excitation spectrum
indicating strong electron-electron interactions.Comment: 12 pages, 4 figure
Host affinity of endophytic fungi and the potential for reciprocal interactions involving host secondary chemistry
PREMISE: Interactions between fungal endophytes and their host plants present useful systems for identifying important factors affecting assembly of host-associated microbiomes. Here we investigated the role of secondary chemistry in mediating host affinity of asymptomatic foliar endophytic fungi using Psychotria spp. and Theobroma cacao (cacao) as hosts. METHODS: First, we surveyed endophytic communities in Psychotria species in a natural common garden using culture-based methods. Then we compared differences in endophytic community composition with differences in foliar secondary chemistry in the same host species, determined by liquid chromatography–tandem mass spectrometry. Finally, we tested how inoculation with live and heat-killed endophytes affected the cacao chemical profile. RESULTS: Despite sharing a common environment and source pool for endophyte spores, different Psychotria host species harbored strikingly different endophytic communities that reflected intrinsic differences in their leaf chemical profiles. In T. cacao, inoculation with live and heat-killed endophytes produced distinct cacao chemical profiles not found in uninoculated plants or pure fungal cultures, suggesting that endophytes, like pathogens, induce changes in secondary chemical profiles of their host plant. CONCLUSIONS: Collectively our results suggest at least two potential processes: (1) Plant secondary chemistry influences assembly and composition of fungal endophytic communities, and (2) host colonization by endophytes subsequently induces changes in the host chemical landscape. We propose a series of testable predictions based on the possibility that reciprocal chemical interactions are a general property of plant–endophyte interactionsPREMISE: Interactions between fungal endophytes and their host plants present useful systems for identifying important factors affecting assembly of host-associated microbiomes. Here we investigated the role of secondary chemistry in mediating host affinity of asymptomatic foliar endophytic fungi using Psychotria spp. and Theobroma cacao (cacao) as hosts. METHODS: First, we surveyed endophytic communities in Psychotria species in a natural common garden using culture-based methods. Then we compared differences in endophytic community composition with differences in foliar secondary chemistry in the same host species, determined by liquid chromatography–tandem mass spectrometry. Finally, we tested how inoculation with live and heat-killed endophytes affected the cacao chemical profile. RESULTS: Despite sharing a common environment and source pool for endophyte spores, different Psychotria host species harbored strikingly different endophytic communities that reflected intrinsic differences in their leaf chemical profiles. In T. cacao, inoculation with live and heat-killed endophytes produced distinct cacao chemical profiles not found in uninoculated plants or pure fungal cultures, suggesting that endophytes, like pathogens, induce changes in secondary chemical profiles of their host plant. CONCLUSIONS: Collectively our results suggest at least two potential processes: (1) Plant secondary chemistry influences assembly and composition of fungal endophytic communities, and (2) host colonization by endophytes subsequently induces changes in the host chemical landscape. We propose a series of testable predictions based on the possibility that reciprocal chemical interactions are a general property of plant–endophyte interaction
Mechanical properties of freely suspended atomically thin dielectric layers of mica
We have studied the elastic deformation of freely suspended atomically thin
sheets of muscovite mica, a widely used electrical insulator in its bulk form.
Using an atomic force microscope, we carried out bending test experiments to
determine the Young's modulus and the initial pre-tension of mica nanosheets
with thicknesses ranging from 14 layers down to just one bilayer. We found that
their Young's modulus is high (190 GPa), in agreement with the bulk value,
which indicates that the exfoliation procedure employed to fabricate these
nanolayers does not introduce a noticeable amount of defects. Additionally,
ultrathin mica shows low pre-strain and can withstand reversible deformations
up to tens of nanometers without breaking. The low pre-tension and high Young's
modulus and breaking force found in these ultrathin mica layers demonstrates
their prospective use as a complement for graphene in applications requiring
flexible insulating materials or as reinforcement in nanocomposites.Comment: 9 pages, 5 figures, selected as cover of Nano Research, Volume 5,
Number 8 (2012
Molecular diversity of arbuscular mycorrhizal fungi and patterns of host association over time and space in a tropical forest
We have used molecular techniques to investigate the diversity and distribution of the arbuscular mycorrhizal (AM) fungi colonizing tree seedling roots in the tropical forest on Barro Colorado Island (BCI), Republic of Panama. In the first year, we sampled newly emergent seedlings of the understory treelet Faramea occidentalis and the canopy emergent Tetragastris panamensis, from mixed seedling carpets at each of two sites. The following year we sampled surviving seedlings from these cohorts. The roots of 48 plants were analysed using AM fungal-specific primers to amplify and clone partial small subunit (SSU) ribosomal RNA gene sequences. Over 1300 clones were screened for random fragment length polymorphism (RFLP) variation and 7% of these were sequenced. Compared with AM fungal communities sampled from temperate habitats using the same method, the overall diversity was high, with a total of 30 AM fungal types identified. Seventeen of these types have not been recorded previously, with the remainder being similar to types reported from temperate habitats. The tropical mycorrhizal population showed significant spatial heterogeneity and nonrandom associations with the different hosts. Moreover there was a strong shift in the mycorrhizal communities over time. AM fungal types that were dominant in the newly germinated seedlings were almost entirely replaced by previously rare types in the surviving seedlings the following year. The high diversity and huge variation detected across time points, sites and hosts, implies that the AM fungal types are ecologically distinct and thus may have the potential to influence recruitment and host composition in tropical forests
Superconducting and Quantum-Effect Devices
Contains reports on six research projects and a list of publications.National Science Foundation Grant DMR 94-02020National Science Foundation Fellowship MIP 88-58764U.S. Air Force - Office of Scientific Research Grant F30602-96-1-0059 Rome LaboratoryDefense Advanced Research Projects Agency/Consortium for Superconducting Electronics Contract MDA 972-90-C-002
Prolonged QTc Interval and Risk of Sudden Cardiac Death in a Population of Older Adults
ObjectivesThis study sought to investigate whether prolongation of the heart rate-corrected QT (QTc) interval is a risk factor for sudden cardiac death in the general population.BackgroundIn developed countries, sudden cardiac death is a major cause of cardiovascular mortality. Prolongation of the QTc interval has been associated with ventricular arrhythmias, but in most population-based studies no consistent association was found between QTc prolongation and total or cardiovascular mortality. Only very few of these studies specifically addressed sudden cardiac death.MethodsThis study was conducted as part of the Rotterdam Study, a prospective population-based cohort study that comprises 3,105 men and 4,878 women aged 55 years and older. The QTc interval on the electrocardiogram was determined during the baseline visit (1990 to 1993) and the first follow-up examination (1993 to 1995). The association between a prolonged QTc interval and sudden cardiac death was estimated using Cox proportional hazards analysis.ResultsDuring an average follow-up period of 6.7 years (standard deviation, 2.3 years) 125 patients died of sudden cardiac death. An abnormally prolonged QTc interval (>450 ms in men, >470 ms in women) was associated with a three-fold increased risk of sudden cardiac death (hazard ratio, 2.5; 95% confidence interval, 1.3 to 4.7), after adjustment for age, gender, body mass index, hypertension, cholesterol/high-density lipoprotein ratio, diabetes mellitus, myocardial infarction, heart failure, and heart rate. In patients with an age below the median of 68 years, the corresponding relative risk was 8.0 (95% confidence interval 2.1 to 31.3).ConclusionsAbnormal QTc prolongation on the electrocardiogram should be viewed as an independent risk factor for sudden cardiac death
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