Comparative Karyotype Analysis of Slugs of the Genus Arion (Gastropoda, Pulmonata, Arionidae)

Comparative Karyotype Analysis of Slugs of the Genus Arion (Gastropoda, Pulmonata, Arionidae). Harbar, A. V., Kadlubovska, N. S., Harbar, D. A. — Karyotypes of four species of the genus Arion were investigated, namely, A. distinctus (2n = 48m + 8sm = 56; FN = 112), A. lusitanicus s. l. (2n = 4 4m + 6 sm + 2st = 52; FN = 104), A. fuscus (n = 26, 2n = 52, FN = 104) and A. fasciatus (n = 29, 2n = 58, FN = 116). Th e karyotype of A. lusitanicus s. l. was identical to those of A. fuscus, A. ater and A. rufus. Th e karyotype of A. fasciatus in the haploid number of chromosomes is identical to another close species — A. circumscriptus (n = 29) from the United Kingdom. Th e identical number of chromosomes in species of the subgenus Arion (A. lusitanicus s. l., A. ater, A. rufus) and species of the subgenus Mesarion (A. fuscus) (n = 26) may be a reason for their merging

THE CONTENT OF PROSTANOIDS AND CYCLOOXYGENASES IN COLON TISSUE IN EXPERIMENTAL ULCERATIVE COLITIS

Introduction. The article examines changes in the content of prostaglandins and cyclooxygenases (COX) in colon tissue in ulcerative colitis induced by 2,4-dinitrobenzene sulfonic acid (DNBS) in a 50% ethanol solution. Based on the obtained results, the authors conclude that changes in the content of the studied parameters, except PGI2, are due to ethanol effect, not DNBS. Both COX isozymes are expressed in normal colon and reduced in ulcerative colitis. The aim. To study the prostanoids (PGE2, PGI2, PGF2α, TBX2 and 8-iso-PGF2α) and COX-1 and -2 contents in colon tissue in experimental ulcerative colitis. Materials and methods. The determination of prostanoids and cyclooxygenases contents in colon tissue by enzyme immunosorbent assay was carried out on three groups of sexually mature laboratory rats of both sexes of the WAG population (1st control group – intrarectal injection of saline; 2nd control group – injection of 50% ethanol; experimental group – injection of DNBS in 50% ethanol). Results. PGE2 and PGI2 contents in colon tissue of experimental group rats were statistically significantly higher compared 1st and 2nd control groups. The content of PGE2 was also increased in 2nd control group versus 1st control one. The increasing PGI2 in 2nd control group versus 1st control was not significant. TBX2 and PGF2α contents in experimental and 2nd control groups were significantly lower compared 1st control. 8-iso-PGF2α (non-enzymatically derived prostanoid) level in experimental group rats was significantly higher compared both controls. 8-iso-PGF2α content in 2nd control group was significantly higher compared 1st one. The content of both COX isoforms in colon tissue in experimental group and 2nd control group rats was significantly lower compared to 1st control group. Conclusions. Both isoforms of COX are expressed in control group colon indicating COX-2 involvement in supporting physiological functions of normal colon tissue. All studied indicators changes, except PGI2, are due to ethanol, not DNBS. Both 50% ethanol and DNBS in 50% ethanol stimulate lipid peroxidation, confirmed by significant increase in 8-iso-PGF2α content. PGE2 and PGF2α contents changes against the background of reduced levels of COX-1 and COX-2 in experimental ulcerative colitis are most likely an adaptive response aimed at maintaining colon homeostasis. PGI2 content changes are due to DNBS, and not to ethanol

Evaluation of the application of not traumatic linking and learning techniques in two races of horses

Existe un conjunto de prácticas que permiten la familiarización del potrillo con el entorno, además de facilitar la detección y el diagnóstico de enfermedades, malformaciones y/o lesiones posparto (Gónzalez del Pino et al, 2009). Se conocen como Técnicas de Vinculación y Aprendizaje no Traumáticas (TVANT) y son realizadas mediante un contacto diario, tanto con el potrillo como con su madre, implicando una revisión habitual a cada individuo desde el día 7 al 14 desde el nacimiento. Nuestra experiencia en raza Criolla ha mostrado que las TVANT producen resultados satisfactorios (Abbiati et al, 2016). El objetivo de este trabajo fue comparar la duración del proceso para las razas Criolla y Silla Argentino en las maniobras de las TVANT.Facultad de Ciencias Veterinaria

The Living Museum is an ethno farm of gray Ukrainian cattle as a model of the location of rural green tourism facilities

The article is devoted to the development of ecological tourism in the agrarian territories of Ukraine, in particular, the creation of an organizational model of a living museum-ethno-farm, exhibits of which are productive gray cattle, whose herds a hundred years ago formed the basis of the economy disappearing. As objects of the tourist business, animals of the gray Ukrainian breed are beautiful to tourists: they have a beautiful appearance that is immediately memorable, have a calm, non-aggressive temperament, and have an excellent ability to get used to pastoral work. Today there are about a thousand animals of the aboriginal gray Ukrainian breed (in two state farms of Ukraine); this breed is recognized as a national heritage and has unique genetic and phenotypic qualities. The proposed model of a living open-air museum includes structural elements that allow creative use of local conditions of the modern Ukrainian village for the location of the ethno-farm and provide a vector of solutions to acute socio-cultural problems that will promote sustainable rural development: stop depopulation, expand to the history of their land, etc. The development of living museums-ethno-farms will reveal and use in the process of creative rethinking of folk traditions accumulated in previous generations invaluable qualities, namely: respect for rural labor and knowledge of the benefits of environmental management in environmental and social challenges

The peculiarities of foreign and native use application in the professional speech of a manager

Гарбар, І. В. Особливості вживання іншомовних та питомих слів у фаховому мовлені менеджера / І. В. Гарбар, А. І. Гарбар, Л. І. Петрович // Гуманіт. вісн. НУК. – Миколаїв : НУК, 2014. – Вип. 7. – С. 27–30.У статті проаналізовано особливості вживання іншомовних та питомих слів у фаховому мовленні менеджера, доцільність заміни іншомовних слів українськими відповідникамиThe article analyses the peculiarities of foreign and native words use in the professional speech of a manager, practicability of foreign words substitution with its Ukrainian equivalents